Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

2021 ◽  
pp. 86-95
Author(s):  
Baiq Maylinda Gemantari ◽  
Fitra Romadhonsyah ◽  
Arief Nurrochmad ◽  
Subagus Wahyuono ◽  
Puji Astuti
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Cytotoxic Activities ◽  
Dpph Assay ◽  
Nitrobenzyl Alcohol ◽  
Layer Chromatography ◽  
Coleus Amboinicus

Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R*,6S*,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.

scholarly journals Isolation and antimicrobial effects of endophytic fungi from Edgeworthia chrysantha

2015 ◽  
Vol 10 (3) ◽  
pp. 529 ◽  
Author(s):  
Huawei Zhang ◽  
Chuanfeng Ruan ◽  
Xuelian Bai
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Fungal Endophytes ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Human Pathogens ◽  
Antimicrobial Effects ◽  
Staphyloccocus Aureus

<p>Ten fungal strains isolated from <em>Edgeworthia chrysantha</em>, one of traditional medicinal plants in China, were evaluated their antimicrobial activities against three human pathogens, <em>Escherichia coli, Staphyloccocus aureus and Candida albicans</em>, and two phytopathogens, <em>Rhizoctonia cerealis</em> and <em>Colletotrichum gloeosporioides</em>. The results indicated that most ethyl acetate extracts of fermentation broth of these fungal endophytes had stronger antimicrobial activities than their fermentation broth. Among these endophytic strains, both fermentation broth and the ethyl acetate extract of strain D showed the strongest inhibitory effects on all pathogens. Strains 5-19 and BZ also exhibited potent antibacterial activities. However, other strains had weak or no antimicrobial effect. This was the first report on the isolation and antimicrobial effects of endophytic fungi from <em>E. chrysantha</em>.   </p><p> </p>

scholarly journals Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 379 ◽  
Author(s):  
Dhurva Prasad Gauchan ◽  
Pratistha Kandel ◽  
Astha Tuladhar ◽  
Ashesh Acharya ◽  
Upendra Kadel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Diffusion Method ◽  
Dry Extract ◽  
Brine Shrimp Lethality Assay ◽  
Fungal Extracts ◽  
Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

scholarly journals New Fluvirucinins C1 and C2 Produced by a Marine Derived Actinomycete

2017 ◽  
Vol 12 (5) ◽  
pp. 1934578X1701200
Author(s):  
Margarida Costa ◽  
Paz Zúñiga ◽  
Ana M Peñalver ◽  
Margrét Thorsteinsdottir ◽  
Marta Pérez ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Marine Sponge ◽  
Fermentation Broth ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
New Type ◽  
The Common

Two new fluvirucin aglycones, named fluvirucinins C1 and C2 (1-2), have been isolated from the ethyl acetate mycelial cake extract of the fermentation broth of a marine sponge-associated actinomycete. Fluvirucinins C1 (1) and C2 (2) represent a new type of 14-membered macrolactam aglycon, structurally related with the common aglycon of the known fluvirucins. Their structures were elucidated on the basis of 1D and 2D NMR analyses, as well as HRESIMS experiments. The antimicrobial and cytotoxic activities of compounds 1 and 2 have been evaluated, but no significant activities found for fluvirucinins C1 and C2.

scholarly journals Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 379
Author(s):  
Dhurva Prasad Gauchan ◽  
Pratistha Kandel ◽  
Astha Tuladhar ◽  
Ashesh Acharya ◽  
Upendra Kadel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Diffusion Method ◽  
Dry Extract ◽  
Brine Shrimp Lethality Assay ◽  
Fungal Extracts ◽  
Taxus Wallichiana

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata, Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively. Conclusions: The crude fungal extracts obtained from endophytes: A. alternata, C. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

scholarly journals Bioactive Compounds from Aspergillus Terreus MP15, an Endophytic Fungus Isolated from Swietenia Macrophylla Leaf

2017 ◽  
Vol 4 (2) ◽  
pp. 107-116
Author(s):  
Olivia Chan Jade Yin ◽  
Darah Ibrahim ◽  
Chong Chai Lee
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Aspergillus Terreus ◽  
Ethyl Acetate Extract ◽  
Bioactive Metabolites ◽  
Partial Purification ◽  
Swietenia Macrophylla ◽  
Gram Positive

Endophytes are important sources for the discovery of bioactive compounds. They are known as potential producers of novel compounds with antimicrobial activity. In this study, it aimed to study the bioactive metabolites present in the ethyl acetate extract from the endophytic fungus Aspergillus terreus MP15 of Swietenia macrophylla leaf. The ethyl acetate extract showed promising antibacterial activity against Gram-positive foodborne bacteria (Bacillus cereus, Bacillus spizizenii, Bacillus subtilis and Staphylococcus aureus) in disc diffusion assay. The extract was then subjected to thin layer chromatography (TLC) using dichloromethane: methanol (9:1) as a mobile solvent system and eleven spots with diverse polarities were obtained. The TLC chromatogram with bioactive spot was localized with bioautography assay and the result showed that the yellow spot with Rf value of 0.523 exhibited promising antibacterial activity against the four Gram-positive bacteria tested. Upon partial purification of the yellow bioactive fraction using column chromatography, the fraction 2a exhibited promising antibacterial activity. The GC-MS analysis of the yellow fraction 2a resulted in the detection of a major compound, di-n-octyl phthalate with 80% matching factor. Therefore, this compound may largely contribute to the antibacterial activity of the fraction and has potential to be food preservative and as a colorant. These results indicate that endophytic fungi isolated from medicinal plants could be a potential source for bioactive compounds.

Study on Limonium aureum Endophytic Fungi and its Antibacterial Activity

2013 ◽  
Vol 634-638 ◽  
pp. 1071-1075 ◽  
Author(s):  
Xiao Feng Liu ◽  
Jiao Ling Qian ◽  
Peng Lin ◽  
Hong Yan Xue ◽  
Lin Yang
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Filter Paper ◽  
Fermentation Broth ◽  
Test Bacterium ◽  
General Method

Objective To research antibacterial activity of Limonium aureum endophytic fungi. Methods The endophytic fungi of Limonium aureum root was isolated by general method. After incubation, fermentation broth and biomass were extracted by ethyl acetate, n-bothanol and ethanol. The bactefiostatic test was experimented with six different bacterium．The method used was filter paper slice tests. Results 21 endophytes were obtained. After bactefiostatic testing, there were 31 samples with antibaceterial activity against at least one test bacterium he MIC of extraction from E101 and E201 against test bacteria was 0.25mg/mL.

Evaluation of In Vitro Antibacterial Activity of Endophytic Fungus from Anemone tomentosa

2014 ◽  
Vol 881-883 ◽  
pp. 484-487 ◽  
Author(s):  
Lin Yang ◽  
Jing Long ◽  
Wei Juan Li ◽  
Ai Mei Yang ◽  
Zhong Duo Yang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Filter Paper ◽  
Endophytic Fungus ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Paper Method

In this study, 23 endophytic fungus were isolated from Anemone tomentosa. The antibacterial activity of the endophytic fungi were tested and analyzed by filter paper method. The results showed that the endophytic fungi from Anemone tomentosa had broad antibiogram and strong inhibited Gram-positive bacteria and Gram-negative bacteria, especially the mycelia extract of root-1, the ethyl acetate and the n-BuOH extracts of root-8. So the endophytic fungi from Anemone tomentosa could be an important resource for developing antimicrobial products.

scholarly journals Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
Fadhillah Fadhillah ◽  
ELFITA ◽  
MUHARNI ◽  
HENI YOHANDINI ◽  
HARY WIDJAJANTI
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Chemical Compound ◽  
The Body ◽  
Dpph Method ◽  
Number Of Microorganisms ◽  
Free Radical Activity ◽  
Swietenia Mahagoni

Abstract. Fadhillah, Elfita, Muharni, Yohandini H, Widjajanti. 2019. Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks. Biodiversitas 20: 2645-2650. Swietenia mahagoni L. Jacq is a medicinal plant that widely used as an antimicrobial, anti-inflammatory, antimutagenic, anticancer, antitumor, and antidiabetic. Several bioactive compounds have been reported to be associated with this plant that supports its treatment activities. Flavonoid content of the S. mahagoni has antioxidant activity. Therefore the plant is widely used to treat degenerative diseases caused by free radical activity in the body. Scientists have proven that the medicinal plants have a symbiotic relationship with the number of microorganisms that can produce bioactive compounds. One of the symbiotic microorganisms is endophytic fungi believed to be associated with every plant on earth. In this paper, we report a secondary metabolite compound from endophytic fungi isolated from S. mahagoni. The endophytic fungi (DMG1-DMG4) were isolated from leaf stalks of S. mahagoni. Antioxidant activity of endophytic fungi extracts was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The chemical compound from the active extract was isolated by the chromatographic method, and the structure was elucidated by spectroscopic methods including UV, IR, NMR 1D (1H-NMR and 13C-NMR), NMR 2D (HMQC and HMBC). Antioxidant activity test showed the endophytic fungus (DMG3) has the highest activity. The chemical compound from DMG3 was identified as 5-hydroxy-2-oxo-2H-piran-4-yl) methyl acetate). The phylogenetic method used to molecular analysis of DMG3 confirmed the fungus as Cladosporium tenuissimum.

scholarly journals Averrhoa bilimbi: A Prospective Source of Bioactive Compounds against Antimicrobial and Cytotoxic Activities

2020 ◽  
Vol 33 (1) ◽  
pp. 179-184
Author(s):  
Md. Abdul Mannan ◽  
Liton Chandra ◽  
A.B.M. Nazmul Islam ◽  
Md. Saddam Hossain ◽  
Md. Kudrat-E-Zahan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Bioactive Compounds ◽  
Brine Shrimp ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Test Methods ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Scavenging Activity

Averrhoa bilimbi fruit has been studied to find out a possible source of bioactive compounds against antimicrobial and cytotoxic activity. To achieve this goal, total phenolic content and total flavonoid content, antioxidant, antimicrobial and cytotoxic activities of five different fractionates such as methanolic crude, petroleum ether, chloroform, ethyl acetate and water fraction of A. bilimbi were investigated by UV-vis spectrophotometry, disc diffusion and brine shrimp lethality test methods simultaneously. Other phytochemicals viz. carbohydrate, tannins, polyphenols, glycosides, etc. were also screened out by conventional test methods concurrently. Total phenolic contents and total flavonoid contents were found to be ranging from 3.807-262.129 mg GAE g–1 and 67.848-1159.061 mg CE g–1, respectively, in all the analyzed fractions. DPPH free radical scavenging activity revealed distinctive IC50 value of 5.63 and 10.26 μg mL–1 in the chloroform and ethyl acetate extractives. Fractionates of petroleum ether and methanolic crude also presented strong cytotoxic potential with the LD50 values of 8.89 and 20.3 μg mL–1 in the brine shrimp lethality bioassay test. Petroleum ether and ethyl acetate fractions exhibited good antimicrobial activity with the zone of inhibition against Bacillus cereus (11 mm and 14 mm) and Escherichia coli (7 mm and 9 mm). Finally, it has been recommended that A. bilmbi fruit might be an effective source of bioactive compounds against antimicrobial and cytotoxic activity as well as antioxidant with potent radical scavenging activity.

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