scholarly journals Therapeutic efficacy of Lactobacillus acidophilus against bacterial isolates from burn wounds

2010 ◽  
pp. 586-591 ◽  
Author(s):  
Mohammed Jebur
Keyword(s):  
Lactobacillus Acidophilus ◽  
Therapeutic Efficacy ◽  
Bacterial Isolates ◽  
Burn Wounds

Changes in bacterial isolates from burn wounds and their antibiograms: A 20-year study (1986–2005)

Burns ◽  
2009 ◽  
Vol 35 (4) ◽  
pp. 553-560 ◽  
Author(s):  
Merlin Guggenheim ◽  
Reinhard Zbinden ◽  
Alexander E. Handschin ◽  
Andreas Gohritz ◽  
Mehmet A. Altintas ◽  
...  
Keyword(s):  
Bacterial Isolates ◽  
Burn Wounds

Lactobacillus Acidophilus Supplementation Exerts A Synergistic Effect on Tacrolimus Efficacy by Restoring Th17/Treg Imbalance in Lupus-Prone Mice via the SIGNR3 Pathway

2020 ◽  
Author(s):  
Da Som Kim ◽  
Youngjae Park ◽  
Jeong-Won Choi ◽  
Sung-Hwan Park ◽  
MI-LA CHO ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Combination Therapy ◽  
Combination Treatment ◽  
Lupus Erythematosus ◽  
Lactobacillus Acidophilus ◽  
Therapeutic Efficacy ◽  
T Cell Subsets ◽  
Systemic Autoimmune Disease

Abstract Background: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterised by tissue-binding autoantibodies and immune complexes. Tacrolimus (Tac), also known as FK506, is an immunosuppressant used in the treatment of lupus; however, it induces T-cell imbalances. Lactobacillus acidophilus (LA) is reported to have therapeutic efficacy in immune-mediated diseases via T-cell regulation. This study investigated whether a combination therapy of LA and Tac improves the therapeutic efficacy of Tac by ameliorating T-cell imbalance in an animal model of SLE. Eight-week-old MRL/Mp-Faslpr (MRL/lpr) mice were orally administered with 5 mg/kg of Tac and/or 50 mg/kg of LA daily for 8 weeks. Caecal microbiota compositions, serum autoantibodies levels, the degree of proteinuria, histological changes in the kidney, and populations of various T-cell subsets in the spleen were analysed. Results: MRL/lpr mice presented with significant gut microbiota imbalances, which were subsequently recovered by the combination treatment of Tac and LA. Double negative T-cells, a pathogenic subset of T-cells, in the peripheral blood and spleens of MRL/lpr mice were significantly decreased by the combination therapy. The combination treatment also reduced serum levels of anti-double-stranded DNA antibodies and immunoglobulin G2a, and renal pathology scores were markedly alleviated. The combination therapy induced Treg cells and decreased Th17 cells both in vitro and in vivo. In vitro treatment with LA induced the production of indoleamine-2,3-dioxygenase, programmed death-ligand 1, and inerleukin-10, which was partially mediated by the induction of the specific intracellular adhesion molecule-3 grabbing non-integrin homolog-related 3 (SIGNR3) receptor. Conclusions: The present findings indicate that LA augments the therapeutic effect of Tac and restores Th17/Treg balance in a murine model of lupus. Accordingly, the combination treatment of Tac with LA could be a promising therapeutic candidate for lupus.

scholarly journals Lactobacillus acidophilus Supplementation Exerts a Synergistic Effect on Tacrolimus Efficacy by Modulating Th17/Treg Balance in Lupus-Prone Mice via the SIGNR3 Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Da Som Kim ◽  
Youngjae Park ◽  
Jeong-Won Choi ◽  
Sung-Hwan Park ◽  
Mi-La Cho ◽  
...  
Keyword(s):  
T Cell ◽  
Combination Therapy ◽  
Combination Treatment ◽  
Lactobacillus Acidophilus ◽  
Therapeutic Efficacy ◽  
Cell Subset ◽  
Treg Cells ◽  
T Cell Subsets ◽  
T Cell Subset

ObjectiveTacrolimus (Tac) is an immunosuppressant used in the treatment of systemic lupus erythematosus (SLE); however, it induces T cell subset imbalances by reducing regulatory T (Treg) cells. Lactobacillus acidophilus (LA) is reported to have therapeutic efficacy in immune-mediated diseases via T cell regulation.MethodsThis study investigated whether a combination therapy of LA and Tac improves the therapeutic efficacy of Tac by modulating T cell subset populations in an animal model of SLE. Eight-week-old MRL/lpr mice were orally administered with 5 mg/kg of Tac and/or 50 mg/kg of LA daily for 8 weeks. Cecal microbiota compositions, serum autoantibodies levels, the degree of proteinuria, histological changes in the kidney, and populations of various T cell subsets in the spleen were analyzed.ResultsMice presented with significant gut dysbiosis, which were subsequently recovered by the combination treatment of Tac and LA. Double negative T cells in the peripheral blood and spleens of MRL/lpr mice were significantly decreased by the combination therapy. The combination treatment reduced serum levels of anti-dsDNA antibodies and Immunoglobulin G2a, and renal pathology scores were also markedly alleviated. The combination therapy induced Treg cells and decreased T helper 17 (Th17) cells both in vitro and in vivo. In vitro treatment with LA induced the production of indoleamine-2,3-dioxygenase, programmed death-ligand 1, and interleukin-10 via the specific intracellular adhesion molecule-3 grabbing non-integrin homolog-related 3 receptor signals.ConclusionThe present findings indicate that LA augments the therapeutic effect of Tac and modulates Th17/Treg balance in a murine model of SLE.

Evodiamine has therapeutic efficacy in ulcerative colitis by increasing Lactobacillus acidophilus levels and acetate production

2020 ◽  
Vol 159 ◽  
pp. 104978
Author(s):  
Meng-Xia Wang ◽  
Li Lin ◽  
Yong-Da Chen ◽  
Yu-Ping Zhong ◽  
Yi-Xuan Lin ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Lactobacillus Acidophilus ◽  
Therapeutic Efficacy ◽  
Acetate Production

Murine immunomodulation of IL-10 and IL-12 induced by new isolates from avian type 2 Lactobacillus acidophilus

2007 ◽  
Vol 53 (8) ◽  
pp. 944-956 ◽  
Author(s):  
Christian Bleau ◽  
Roland Savard ◽  
Lucie Lamontagne
Keyword(s):  
Lactobacillus Acidophilus ◽  
Mapk Pathway ◽  
Lactobacillus Rhamnosus ◽  
Peritoneal Macrophages ◽  
Lymphoid Cells ◽  
Mitogen Activated Protein Kinase ◽  
Bacterial Isolates ◽  
Necrosis Factor Alpha ◽  
Mitogen Activated Protein

Several bacterial and immunogenic factors are involved in the host response to probiotic strains of Lactobacillus . Here, we report the isolation of new intestinal lactobacilli from chicken, with different immunomodulating properties on lymphoid cells from SJL and C57BL/6 mice. Analysis of biochemical markers in the Lactobacillus acidophilus CBA4P, CBA3P, and TPA3P isolates reveal that these bacterial isolates belong to the type 2 prototype, although they differ from each other. The effect of conditioned media (CM) from SJL- and C57BL/6-derived peritoneal macrophages incubated with the 3 sonicated bacterial isolates from chicken, as well as with Lactobacillus rhamnosus 9595, Escherichia coli lipopolysaccharide, or Staphylococcus aureus peptidoglycan were compared. Our results show that the CM of macrophages from C57BL/6 and SJL mice treated with the CBA4P isolate stimulated syngeneic splenic lymphocytes at a level similar to the one induced with CM from peptidoglycan-stimulated macrophages. In contrast, the CM from TPA3P- and CBA3P-treated macrophages promoted low or no stimulation of lymphoid cells. Incubation of splenic cells with CM from macrophages treated with L. rhamnosus or TPA3P led to a relative decrease in the percentages of splenic CD4+ T cells, whereas the relative percentages of B cells increased. The CBA4P and CBA3P isolates induced higher levels of gamma interferon when compared with the TPA3P isolate. The effects of the lactobacilli isolates differed according to the mouse strain used but correlated with the production of macrophagic tumor necrosis factor alpha and interleukins 6, 10, and 12 and with the modulation of the p38 mitogen-activated protein kinase (MAPK). Taken together, these results indicate that the immunomodulating properties of the new L. acidophilus isolates depend on their capacity to induce production of interleukins 10 and 12 by macrophages, which is under genetic control and depends on the p38 MAPK pathway.

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PENGHASIL INHIBITOR ENZIM HMG-KoA REDUKTASE DARI BEKASAM SEBAGAI AGEN PEREDUKSI KOLESTEROL

2015 ◽  
Vol 35 (03) ◽  
pp. 309 ◽  
Author(s):  
Rinto Rinto ◽  
Ratih Dewanti ◽  
Sedarnawati Yasni ◽  
Maggy Thenawidjaja Suhartono
Keyword(s):  
Lactobacillus Acidophilus ◽  
Enzyme Inhibitor ◽  
Cholesterol Biosynthesis ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Hmg Coa Reductase ◽  
Identification Of Bacteria ◽  
Coa Reductase ◽  
Inhibition Ability ◽  
Selection Of

The purpose of this research was to obtain statins producer bacteria as a HMG-CoA reductase (HMGR) enzyme inhibitor to reduced cholesterol biosynthesis. Stages of this research were the isolation of compactin and lovastatin resistant bacteria, statin production, analysis of culture extracts to inhibition of HMG-CoA reductase and identification of bacteria. The results showed that the 20 isolates of compactin and lovastatin resistant bacteria, there are 5 bacterial isolates produced statins. They were L3.3.4; C3.4.2; C3.3.5; C3.4.4 and L3.3.3; with the statins content were 9.491;1.536; 0.065; 0.060; and 0.040 ppm. Selection of the 5 bacterial isolates resulted 2 bacteria which had inhibition ability to HMGR enzyme activity. They were Lactobacillus acidophilus and Lactobacillus delbruckii sp. delbruckii with inhibitory ability were 66.67% and 58.33%, respectively.Keywords: L. acidophilus, L. delbrucki, HMGR inhibitor, cholesterol, bekasamABSTRAKPenelitian ini bertujuan memperoleh bakteri penghasil statin sebagai inhibitor enzim HMG-KoA reduktase (HMGR), penghambat sintesis kolesterol. Tahapan penelitian yang dilakukan adalah isolasi bakteri yang resisten terhadap compactin dan lovastatin, produksi statin, uji penghambatan ekstrak dari kultur bakteri terhadap HMG-KoA reduktasedan identifikasi bakteri. Hasil penelitian menunjukan bahwa dari 20 isolat bakteri yang resisten terhadap compactin maupun lovastatin, terdapat 5 isolat bakteri yang potensial menghasilkan statin, yaitu isolat L3.3.4; C3.4.2; C3.3.5; C3.4.4 dan L3.3.3; dengan kandungan statin berturut-turut adalah 9.491; 1,536; 0,065, 0,060, dan 0,040 ppm. Seleksiterhadap 5 isolat menghasilkan 2 bakteri yang mempunyai kemampuan penghambatan terhadap aktivitas enzim ¸Â•ÝWtu Lactobacillus acidophilus dan Lactobacillus delbruckii sp. delbruckii dengan kemampuan penghambatan  berturut-turut adalah 66,67% dan 58,33%.Kata kunci: L. acidophilus, L. delbrucki, inhibitor HMGR, kolesterol, bekasam 

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