Superior Performance of Magnetic Nanoparticles for Entrapment and Fixation of Bovine Serum Albumin In-Vitro

BACKGROUND: In recent years, extensive studies have been performed on magnetite nanoparticles (MNPs) and their applications, which have shown the current project to be one of the major applications by laboratory results.METHODS: The nanoparticles synthesized in this project were deposited by the co-precipitation method, which structure was identified by analyzers such as SEM, FT-IR, and EDX. The aim of this project is the adsorption and fixation of biomolecule (BSA (bovine serum albumin) protein on the surface of magnetic nanoparticles.RESULTS: The adsorption results by electrophoresis and spectrophotometric analyzers showed an absorption rate above 55% ie; 55% of the protein is fixed on the MNPs nanoparticles. This absorption is due to the high level of functionality of magnetic nanoparticles for adsorption of protein. The results of the EDX analysis also show the possible electrostatic bonding between the nanoparticles and the protein, this is derived from –OH with –NH2 groups of the nanobiocompound (MNPs /protein). After bonding, the two are easily separated.CONCLUSION: In this project, the Fe3O4 nanoparticles was synthesized and identified by SEM, FT-IR, and EDX analyzers and finally reacted with the BSA protein (for the absorption of protein on MNPs) under experimental conditions at a standard temperature of 25° C. The results showed that about 55% of the protein was fixed on magnetic nanoparticles.

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Exploring the interaction of l-cysteine capped CuS nanoparticles with bovine serum albumin (BSA): a spectroscopic study

RSC Advances ◽

10.1039/c6ra03583c ◽

2016 ◽

Vol 6 (63) ◽

pp. 58288-58295 ◽

Cited By ~ 32

Author(s):

S. Prasanth ◽

D. Rithesh Raj ◽

T. V. Vineeshkumar ◽

Riju K. Thomas ◽

C. Sudarsanakumar

Keyword(s):

Particle Size ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Copper Sulfide ◽

Average Particle Size ◽

Precipitation Method ◽

Average Particle ◽

Spectroscopic Techniques ◽

Co Precipitation

l-Cysteine capped copper sulfide nanoparticles with an average particle size of 6 nm were synthesized using a chemical co-precipitation method and their interactions with bovine serum albumin were explored using various spectroscopic techniques.

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A Precipitin-like Reaction of the Hemolymph of the Lobster Homarus americanus

Journal of the Fisheries Research Board of Canada ◽

10.1139/f69-127 ◽

1969 ◽

Vol 26 (5) ◽

pp. 1392-1397 ◽

Cited By ~ 10

Author(s):

James E. Stewart ◽

Diane M. Foley

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Serum Proteins ◽

Homarus Americanus ◽

Test Period ◽

Fluorescent Material

The levels of fluorescent material in the hemolymph of lobsters injected with serum proteins from lobster hemolymph labelled with fluorescein remained relatively constant over a 6-day test period; the levels in lobsters injected with bovine serum albumin labelled with fluorescein declined rapidly. A precipitin-like reaction was observed when lobster hemolymph serum was titrated with bovine serum albumin in vitro.

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Preparation and in vitro photodynamic activities of novel axially substituted silicon (IV) phthalocyanines and their bovine serum albumin conjugates

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2006.01.075 ◽

2006 ◽

Vol 16 (9) ◽

pp. 2450-2453 ◽

Cited By ~ 42

Author(s):

Xiong-Jie Jiang ◽

Jian-Dong Huang ◽

Yu-Jiao Zhu ◽

Fen-Xiang Tang ◽

Dennis K.P. Ng ◽

...

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum

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Amuchina Gel Xgerm hand rub in vitro virucidal activity against SARS-CoV-2

Future Microbiology ◽

10.2217/fmb-2021-0128 ◽

2021 ◽

Author(s):

Alessandra Capezzone de Joannon ◽

Angela Testa ◽

Natalie Falsetto ◽

Michela Procaccini ◽

Lorella Ragni

Keyword(s):

Detection Limit ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Virucidal Activity ◽

European Standard ◽

Enveloped Viruses ◽

Prevention Measure ◽

Study Product

Aim: Ethanol is highly effective at inactivating enveloped viruses, including SARS-CoV-2. The aim of this study is to evaluate the virucidal activity of Amuchina Gel Xgerm (74% ethanol) against SARS-CoV-2, according to the European Standard EN14476:2013+A2:2019. Materials & methods: Virucidal activity of the study product was evaluated against SARS-CoV-2 strain USAWA1/2020 in suspension, in the presence of 0.3 g/l of bovine serum albumin. Results: The log10 reduction of SARS-CoV-2 in the presence of bovine serum albumin was ≥4.11 ± 0.12 after 30 s of exposure to the study product (80% dilution). Cytotoxicity was observed in the 100 dilution, affecting the detection limit by 1 log10. Conclusion: Virucidal activity against SARS-CoV-2 supports the effectiveness of this alcohol-based formulation as a prevention measure for COVID-19 illness.

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Evaluación del método dilución neutralización aplicado a un desinfectante según la Norma Técnica Colombiana 5473 de 2007

Universitas Scientiarum ◽

10.11144/javeriana.sc17-1.aotd ◽

2012 ◽

Vol 17 (1) ◽

pp. 72

Author(s):

Janeth Arias-Palacios ◽

Libardo Hernandez-Esquivel ◽

Juan Carlos Marín-Díaz ◽

Natalia Navarro-Peña ◽

Natalia Santos-Arévalo

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Key Words ◽

Bactericidal Activity ◽

Bovine Serum ◽

Enterococcus Hirae ◽

Experimental Conditions ◽

Logarithmic Reduction

Objective. Evaluate the dilution-neutralization method proposed in the Colombian Technical Norm 5473/07, by using a gel, alcoholbased disinfectant. Materials and methods. This study was done using Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 6538, and Enterococcus hirae ATCC 10541 as the assay microorganisms. The study was carried out at 20±1°C as obligatory temperature and additionally at 36±1°C. Four contact times between microorganisms and the disinfectant were evaluated (0, 2, 5 and 10 minutes). The assay was done both under clean conditions (0.3 g/L of bovine serum albumin), and unclean conditions (3 g/L of bovine serum albumin and 3g/L of sheep erythrocytes). Results. The implementation of this method produced precise results in all of the six repetitions used during the assay. The obtained results demonstrated a logarithmic reduction higher than five, demonstrating the bactericidal activity exerted by the disinfectant on the control microorganisms. The established experimental conditions and methodology did not affect negatively the growth of any of the strains of microorganisms. Similarly, the neutralizing used did not inhibit the development of the microorganisms of the assay. Conclusions. The method was verified by means of the fulfillment of the limits set by the rule. Our results suggest that the method evaluated by means of the implementation of the protocol established in the Colombian Technical Norm 5473/07, allows evaluating the effectiveness of a disinfectant under selected and controlled experimental conditions. Key words: disinfection, clean conditions, unclean conditions, dilution-neutralization method, logarithmic reduction.

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