Verocytotoxigenic Escherichia coli O157 and Non- O157 Serogroups in Human Faecal Samples in Northeast Nigeria

2011 ◽  
Vol 18 (2) ◽  
Author(s):  
AE Moses ◽  
BE Bassey ◽  
AN Umoh
Keyword(s):  
Escherichia Coli ◽  
Faecal Samples ◽  
Northeast Nigeria

Prevalence and risk-factor analysis of Shiga toxigenic Escherichia coli in faecal samples of organically and conventionally farmed dairy cattle

2005 ◽  
Vol 109 (1-2) ◽  
pp. 37-45 ◽  
Author(s):  
Peter Kuhnert ◽  
Christoph R. Dubosson ◽  
Markus Roesch ◽  
Esther Homfeld ◽  
Marcus G. Doherr ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Factor Analysis ◽  
Risk Factor ◽  
Dairy Cattle ◽  
Risk Factor Analysis ◽  
Faecal Samples

scholarly journals A novel report of colistin-resistant Escherichia coli carrying mcr-1 gene from animal and human feacal samples in Nigeria

2018 ◽  
Vol 1 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Olugbenga A. Olowe ◽  
Rita A. Olowe ◽  
Adeolu S. Oluremi ◽  
Olusolabomi J. Adefioye
Keyword(s):  
Escherichia Coli ◽  
Animal Husbandry ◽  
Multiple Drug ◽  
Colistin Resistance ◽  
Pcr Assay ◽  
Southwestern Nigeria ◽  
E Coli ◽  
Multiple Drug Resistant ◽  
Microbiological Methods ◽  
Faecal Samples

Background: The mobilized colistin resistance (m cr)-1 gene confers transferable colistin resistance. Reports of mcr-1-positive Escherichia coli (MCRPE) have attracted substantial attention. However, in Nigeria, there is no report of mcr-1 gene resistance. Since colistin is a last resort for multiple drug-resistant isolates, this study therefore report the prevalence of mcr-1 gene among E. coli isolated from human and animal sources. Methods: Out of a total of 280 samples collected from animal and hum an faecal samples from selected farms in Oyo and Osun States, Southwestern Nigeria between July 2015 and June 2016, 60 E. coli were identified using standard microbiological methods. The mcr-1 gene was detected in the isolates by conventional PCR assay. Results: The m cr-1 gene was low and not statistically significant (p≥0.05). It was detected in 5 (8.3%) of 60 E. coli isolates (4= animals; 1= human) Conclusion: This study is the first report of mcr -1 gene from E. coli from human and animal sources in Nigeria. This calls for urgent caution in the use of colistin in animal husbandry.

scholarly journals Sampling considerations for herd-level measurement of faecal Escherichia coli antimicrobial resistance in finisher pigs

1999 ◽  
Vol 122 (3) ◽  
pp. 485-496 ◽  
Author(s):  
R. H. DUNLOP ◽  
S. A. McEWEN ◽  
A. H. MEEK ◽  
R. M. FRIENDSHIP ◽  
W. D. BLACK ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Membrane Filter ◽  
Filter Method ◽  
Animal Testing ◽  
E Coli ◽  
Level Measurement ◽  
Faecal Samples ◽  
Membrane Filter Method ◽  
Estimate Prevalence

The objective of this study was to determine the most efficient means of sampling faeces of finisher pigs for accurate and precise farm-level estimates of antimicrobial resistance among faecal Escherichia coli. Resistance to tetracycline and gentamicin of 8250 isolates of E. coli from 55 finisher pigs on one farm was measured with a hydrophobic grid membrane filter method. The between-pig, within-pen component of variance in resistance was large (97·5%), while between-pen, within-room and between-room components were small (2·5% and 0%, respectively). Using these resistance data, the abilities of two sampling strategies to estimate prevalence were modelled with a Monte Carlo ‘bootstrap’ procedure. Compositing faecal samples from several pigs before testing produced unbiased and precise estimates of prevalence and is simpler technically than individual animal testing.

scholarly journals Sorbitol-fermenting Shiga toxin-producing Escherichia coli O157: indications for an animal reservoir

2005 ◽  
Vol 134 (4) ◽  
pp. 719-723 ◽  
Author(s):  
D. ORTH ◽  
K. GRIF ◽  
M. P. DIERICH ◽  
R. WÜRZNER
Keyword(s):  
Escherichia Coli ◽  
Clinical Diagnosis ◽  
Shiga Toxin ◽  
Haemolytic Uraemic Syndrome ◽  
Stool Specimen ◽  
Farm Animals ◽  
Molecular Characteristics ◽  
Escherichia Coli O157 ◽  
Animal Reservoir ◽  
Faecal Samples

This study investigates a sorbitol-fermenting enterohaemorrhagic Escherichia coli (SF EHEC) O157 infection in a farmer's family in the Austrian province of Salzburg. The investigation commenced after a 10-month-old boy was admitted to hospital with the clinical diagnosis of a haemolytic–uraemic syndrome (HUS) and his stool specimen grew SF EHEC O157:H−. In a subsequent environmental survey, a stool specimen of the 2-year-old brother and faecal samples of two cattle from the family's farm were also found to be positive for SF EHEC O157:H−. All four isolates had indistinguishable phenotypic and molecular characteristics and were identical to the first strain detected in Bavaria in 1988. Despite identical isolates being demonstrated in Bavaria after 1988, and until this report, increased surveillance in neighbouring Austria had not found this organism. We propose that the strain may have recently spread from Bavaria to Austria. Although SF EHEC O157:H− strains are still rare, they may represent a considerable health threat as they can spread from farm animals to humans and between humans.

Evaluation of a PCR detection method for Escherichia coli O157:H7/H- bovine faecal samples

2006 ◽  
Vol 42 (4) ◽  
pp. 386-391 ◽  
Author(s):  
D. Al-Ajmi ◽  
J. Padmanabha ◽  
S.E. Denman ◽  
R.A. Gilbert ◽  
R.A.M. Al Jassim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Detection Method ◽  
Pcr Detection ◽  
Escherichia Coli O157 ◽  
Faecal Samples

scholarly journals First isolation of Escherichia coli O157:H7 from faecal and milk specimens from Anatolian water buffaloes (Bubalus bubalus) in Turkey

2008 ◽  
Vol 79 (4) ◽  
Author(s):  
E. Seker ◽  
H. Yardimci
Keyword(s):  
Escherichia Coli ◽  
Raw Milk ◽  
Escherichia Coli O157 ◽  
Water Buffaloes ◽  
E Coli ◽  
Milk Samples ◽  
Cultural Methods ◽  
Faecal Samples

Three hundred rectal faecal samples and 213 raw milk samples obtained from the tanks and containers were examined using standard cultural methods. Escherichia coli O157:H7 was isolated from 11 (3.7 %) of 300 faecal samples and 3 (1.4 %) of 213 raw milk samples. It was determined that 8 (73 %) of E. coli O157:H7 strains isolated from faecal samples originated from water buffaloes younger than 2 years of age and 3 (27 %) from 2-year-old and older water buffaloes. This is the 1st isolation of Escherichia coli O157:H7 from faecal and milk samples of water buffaloes in Turkey.

Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of broilers

2009 ◽  
Vol 138 (3-4) ◽  
pp. 339-344 ◽  
Author(s):  
Daniela Costa ◽  
Laura Vinué ◽  
Patricia Poeta ◽  
Ana Cláudia Coelho ◽  
Manuela Matos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Faecal Samples

scholarly journals Detection of enteroaggregative Escherichia coli in faecal samples from patients in the community with diarrhoea

2006 ◽  
Vol 55 (11) ◽  
pp. 1493-1497 ◽  
Author(s):  
Claire Jenkins ◽  
Mathias Tembo ◽  
Henrik Chart ◽  
Tom Cheasty ◽  
Geraldine A. Willshaw ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Faecal Samples
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