scholarly journals Antiproliferative effects of isoprenoids from Sarcophyton glaucum on breast cancer MCF-7 cells

2017 ◽  
Vol 16 (3) ◽  
pp. 501 ◽  
Author(s):  
Mohamed A Ghandourah ◽  
Walied M Alarif ◽  
Ahmed Abdel-Lateff ◽  
Khalid O Al-Footy ◽  
Mohamed Halid ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antiproliferative Effects ◽  
Sarcophyton Glaucum ◽  
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Novel dodecyl-containing azido and glucuronamide-based nucleosides exhibiting anticancer potential

2019 ◽  
Vol 91 (7) ◽  
pp. 1085-1105 ◽  
Author(s):  
Nuno M. Xavier ◽  
Rita Goncalves-Pereira ◽  
Radek Jorda ◽  
Denisa Hendrychová ◽  
M. Conceição Oliveira
Keyword(s):  
Breast Cancer ◽  
Myeloid Leukemia ◽  
K562 Cells ◽  
Apoptosis Induction ◽  
Immunoblotting Analysis ◽  
Single Digit ◽  
Standard Drug ◽  
Antiproliferative Effects ◽  
Associated Proteins ◽  
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Abstract The synthesis and anticancer evaluation of new series of nucleosides constructed on 5/6-azidoglycosyl or glucuronamide moieties and containing an O- or an N-dodecyl chain, respectively, are disclosed. Based on our previous results, their structures were planned to preclude them to act via a similar metabolic pathway than that of clinically used nucleoside antimetabolites, against which cancer cells frequently acquire resistance. Xylo and gluco-configured 5/6-azido-1,2-di-O-acetyl furanosyl and pyranosyl donors containing a 3-O-dodecyl group were synthesized from diacetone-d-glucose and were subsequently coupled with silylated uracil or 2-acetamido-6-chloropurine. N-Dodecyl glucuronamide-based nucleosides were accessed from acetonide-protected glucofuranurono-6,3-lactone, which was converted in few steps into O-benzylated 1,2-di-O-acetyl furanuronamide or pyranuronamide derivatives to undergo further N-glycosylation. Both types of nucleosides demonstrated notorious antiproliferative effects in chronic myeloid leukemia (K562) and in breast cancer (MCF-7) cells. The most potent molecules were a 6ʹ-azidoglucopyranosyl N7-linked purine nucleoside and glucofuranuronamide derivatives comprising N1-linked uracil and N7-linked purine units with activities in the single-digit micromolar order of concentration against both cell lines. Their GI50 values in MCF-7 cells were similar or ca. 3-fold lower than that of the standard drug 5-fluorouracil. Cell cycle studies and immunoblotting analysis of apoptosis-associated proteins in treated K562 cells indicated that the antiproliferative effect of the most effective nucleosides is based on apoptosis induction.

scholarly journals Analogs of GnRH-I and GnRH-II inhibit epidermal growth factor-induced signal transduction and resensitize resistant human breast cancer cells to 4OH-tamoxifen

2005 ◽  
Vol 153 (4) ◽  
pp. 613-625 ◽  
Author(s):  
Andreas R Günthert ◽  
Carsten Gründker ◽  
Agnes Olota ◽  
Julia Läsche ◽  
Nicola Eicke ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Antiproliferative Effects ◽  
Epidermal Growth ◽  
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About 50–64% of human breast cancers express receptors for GnRH-I. Direct antiproliferative effects of analogs of GnRH-I on human breast cancer cell lines have been shown. They are at least in part mediated by antagonizing growth promoting effects of estradiol, epidermal growth factor (EGF) or insulin-like growth factor. Recently, expression of a putative receptor for GnRH-II in human tissues was demonstrated. Antiproliferative effects of GnRH-II in human endometrial and ovarian cancer cells were shown not to be mediated through the GnRH-I receptor. Now we demonstrate direct anti-proliferative effects of the GnRH-I analog Triptorelin and the GnRH-II analog [d-Lys6]GnRH-II in MCF-7 and T47D human breast cancer cells expressing GnRH-I receptors and putative GnRH-II receptors. Pretreatment with Triptorelin or [d-Lys6]GnRH-II blocked EGF-induced autophosphoryla-tion of EGF receptor and activation of mitogen-activated protein kinase (extracellular-signal-regulated kinase 1/2 (ERK1/2)) in these cells. In sublines of MCF-7 and T47D cells, which were developed to be resistant to 4OH-tamoxifen, HER-2/p185 was overexpressed. Pretreatment of these cell lines with Triptorelin or [d-Lys6]GnRH-II completely abolished resistance to 4OH-tamoxifen, assessed by 4OH-tamoxifen-induced apoptosis. Analogs of GnRH-I and GnRH-II counteract EGF-dependent signal transduction in human breast cancer cells with expression of receptors for GnRH-I and GnRH-II. Through this mechanism, they probably reverse acquired resistance to 4OH-tamoxifen mediated through overexpression or activation of receptors of the c-erbB family.

scholarly journals Anastatica hierochuntica (L.) methanolic and aqueous extracts exert antiproliferative effects through the induction of apoptosis in MCF-7 breast cancer cells

2020 ◽  
Vol 28 (8) ◽  
pp. 985-993
Author(s):  
Saranya Rameshbabu ◽  
Safia A. Messaoudi ◽  
Zeyad Ibrahim Alehaideb ◽  
Mohammed Syed Ali ◽  
Anuradha Venktraman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Aqueous Extracts ◽  
Antiproliferative Effects ◽  
Induction Of Apoptosis ◽  
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Apoptotic and antiproliferative effects of Stigmast-5-en-3-ol from Dendronephthya gigantea on human leukemia HL-60 and human breast cancer MCF-7 cells

2018 ◽  
Vol 52 ◽  
pp. 297-305 ◽  
Author(s):  
I.P. Shanura Fernando ◽  
K.K. Asanka Sanjeewa ◽  
Yong-Seok Ann ◽  
Chang-ik Ko ◽  
Seung-Hong Lee ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Human Leukemia ◽  
Human Breast ◽  
Antiproliferative Effects ◽  
Dendronephthya Gigantea ◽  
Mcf 7

scholarly journals Comparison of Anticancer Activity of Dorycnium pentaphyllum Extract on MCF-7 and MCF-12A Cell Line: Correlation with Invasion and Adhesion

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 671
Author(s):  
Gözde Koygun ◽  
Emine Arslan ◽  
Gökhan Zengin ◽  
Giustino Orlando ◽  
Claudio Ferrante
Keyword(s):  
Breast Cancer ◽  
Lipid Peroxidation ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Bioinformatics Analysis ◽  
Biological Activities ◽  
Xtt Assay ◽  
Cytotoxic Effects ◽  
Antiproliferative Effects ◽  
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Dorycnium pentaphyllum subsp. haussknechtii is an important medicinal plant in several countries, including Turkey. This study aimed to evaluate the cytotoxicity of a crude extract of D. pentaphyllum subsp. haussknechtii against different breast cell lines to determine invasion, adhesion, and lipid peroxidation. The cytotoxic effects on MCF-7 breast cancer and MCF-12A as the immortalized cell line were examined by the XTT assay. Invasion and adhesion studies were performed according to the manufacturer’s kit procedure to IC50 values for 48 h. Lipid peroxidation was measured in the MCF-7 cell. A bioinformatics analysis was conducted to unravel the mechanism of action underlying antiproliferative effects, as well. According to XTT results, the tested extract showed a time- and a concentration-dependent cytotoxic effect. The most effective concentration was 100.5 µg/mL (48 h), which was selected for biological activities, such as apoptotic activity, invasion, adhesion, and lipid peroxidation assays. The extract caused tumoral cell death, and it did not have a cytotoxic effect on healthy human breast cells. Duplication times and measurement of CI analyses of cells were performed using the real-time cell analysis system xCELLigence. Finally, the bioinformatics analysis indicated the prominent role of quercetin as an extract component exerting a key role in the observed antiproliferative effects. This was supported by the micromolar/submicromolar affinity of quercetin towards proto-oncogene serine/threonine–protein kinase (PIM-1) and hematopoietic cell kinase (HCK), both involved in breast cancer. Altogether, our findings proposed that the extraction of the plant can be an effective strategy to isolate biomolecules with promising cytotoxic effects against breast cancer cells.

Antiproliferative effects of pomegranate extract in MCF-7 breast cancer cells are associated with reduced DNA repair gene expression and induction of double strand breaks

2013 ◽  
Vol 53 (6) ◽  
pp. 458-470 ◽  
Author(s):  
Amit B. Shirode ◽  
Prasad Kovvuru ◽  
Sridar V. Chittur ◽  
Susanne M. Henning ◽  
David Heber ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Dna Repair ◽  
Breast Cancer Cells ◽  
Double Strand Breaks ◽  
Repair Gene ◽  
Antiproliferative Effects ◽  
Strand Breaks ◽  
Dna Repair Gene ◽  
Mcf 7

Combination therapy of calcitriol inhibits the proliferation of breast cancer cells: new concept of nonclassical function of calcitriol

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Khuzama A. Aljunidee ◽  
Sanaa K. Bardaweel
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Breast Cancer Cells ◽  
Induction Effect ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antiproliferative Effects ◽  
Anticancer Effects ◽  
Apoptotic Induction ◽  
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Abstract Objectives To evaluate the anticancer effects of calcitriol and cholecalciferol against different cell lines of breast cancer in monotherapy settings and in combination with raloxifene. Methods The antiproliferative, anti-migratory, and apoptotic induction effects were assessed by MTT, wound healing, and flow cytometry assays, respectively. Results Calcitriol and cholecalciferol exhibited antiproliferative effects against T47D, MCF-7, and MDA-MB-231 in a time and concentration-dependent manner. The IC50 values of calcitriol were in the range of 0.05–0.25 μM while that for cholecalciferol were in the range of 3–100 μM. Furthermore, the results showed that calcitriol and cholecalciferol exhibited anti-migratory effects on MDA-MB-231, an apoptotic induction effect on MCF-7 cells, and a synergistic effect when combined with raloxifene. Conclusions Calcitriol and cholecalciferol exhibited anticancer effects and may be used as chemosensitizers.

miR-27a-mediated antiproliferative effects of metformin on the breast cancer cell line MCF-7

2016 ◽  
Author(s):  
Wei Zhao ◽  
Xiaohui Zhang ◽  
Jia Liu ◽  
Bei Sun ◽  
Hua Tang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Antiproliferative Effects ◽  
Mcf 7
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