EFFECT OF LOW DOSE GAMMA IRRADIATION ON GROWTH AND AFLATOXIN PRODUCTION BY ASPERGILLUS PARASITICUS1

1974 ◽  
Vol 37 (8) ◽  
pp. 430-434 ◽  
Author(s):  
L. B. Bullerman ◽  
T. E. Hartung
Keyword(s):  
Standard Deviation ◽  
Low Dose ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
The Other ◽  
Subsequent Growth ◽  
Irradiation Level ◽  
Yeast Extract Sucrose ◽  
Stimulation Of

Spores and growing vegetative mycelia of Aspergillus parasiticus strains NRRL 2999 and NRRL 3000 were irradiated at 100 and 200 Krad, and the effects on growth and aflatoxin production in yeast-extract sucrose (YES) broth were measured. Irradiation of growing mycelia reduced subsequent growth in YES broth by a greater amount than irradiation of spores. Irradiation of spores at 100 Krad resulted in more B1 and G1 production by strain NRRL 2999 than the non-irradiated control, however, strain NRRL 3000 produced less aflatoxins B1 and G1 after irradiation at 100 Krad than its non-irradiated control. Spores of both strains irradiated at 200 Krad produced less aflatoxins B1 and G1 than non-irradiated controls. Irradiation of growing vegetative mycelia of both strains at 100 and 200 Krad resulted in a definite decline in both aflatoxins B1 and G1 in subsequent cultures at each irradiation level. Apparent stimulation of production of both B1 and G1 occurred after irradiation of spores of strain NRRL 2999 at 100 Krad. However, the variation of the values as determined by the standard deviation was such that one would conclude that no differences existed among means. The apparent stimulation was slight and of much less magnitude than that which has been reported by other investigators using A. flavus. No stimulation of toxin production was observed with the other strain when grown from irradiated spores or with either strain when vegetative mycelia were irradiated.

Growth and Aflatoxin Production by Aspergillus parasiticusNRRL 2999 as Affected by the Fungicide Iprodione1

1993 ◽  
Vol 56 (8) ◽  
pp. 718-721 ◽  
Author(s):  
AGUSTIN A. ARINO ◽  
LLOYD B. BULLERMAN
Keyword(s):  
Yeast Extract ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Mold Growth ◽  
Yeast Extract Sucrose ◽  
Different Levels ◽  
Mycelial Development

Spores of Aspergillus parasiticus strain NRRL 2999 were inoculated into yeast extract sucrose broth containing different levels of iprodione (0, 1, 3, 5, 10, 15, and 20 (μg/ml) and incubated at 25°C for 4, 7, 10, 14, and 21 d. Iprodione inhibited mold growth and subsequent toxin production, beginning at the 5 μg/g level up to 7 d of incubation. Results showed that as the iprodione level increased, more time was required by the organism to initiate mycelial development. At any given time, the lower the iprodione level, the more dry mycelial weight and aflatoxin production (B1, B2, G1, and G2) were observed.

Effects of Nutrients and Inhibitors in Olives on Aflatoxigenic Molds1

1987 ◽  
Vol 50 (11) ◽  
pp. 959-963 ◽  
Author(s):  
ABDELMAJID MAHJOUB ◽  
LLOYD B. BULLERMAN
Keyword(s):  
Amino Acids ◽  
Yeast Extract ◽  
Aflatoxin B1 ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Aqueous Extracts ◽  
Poor Growth ◽  
Extensive Growth ◽  
Yeast Extract Sucrose

Growth and aflatoxin production by Aspergillus parasiticus NRRL 2999 and Aspergillus flavus NRRL 6555 were studied on fresh olives, fresh olives supplemented with nutrients, and fresh olives treated with heat, lye, and freezing temperatures. Studies were also done on yeast extract sucrose agar (YESA) either mixed with chopped fresh olives or made with aqueous extracts of fresh and treated olives. Samples were incubated at 25°C for 7 d. Olive paste supplemented with zinc and sucrose supported little growth and no aflatoxin B1 production. Amino acids, yeast extract, and a combination of zinc, carbohydrate, and amino acids exhibited extensive growth and moderate amounts of aflatoxin. Fresh and frozen olive pastes supported poor growth and no aflatoxin production. Heat- and lye-treated olives supported extensive growth and little aflatoxin production. Heavy growth and moderate amounts of aflatoxin B1 were supported by YES A mixed with olive pastes. YES A made with aqueous extracts of olives supported extensive growth and moderate toxin production, except on YES A made with extract from frozen olives which exhibited poor growth and low toxin amounts. A. flavus grew similarly to A. parasiticus but was unable to produce any aflatoxin except on heat- and lye-treated olives, where traces were detected. Olives are a poor substrate for mold development and may contain inhibiting substances against growth and aflatoxin production.

Effects of Potassium Sorbate on Growth and Aflatoxin Production by Aspergillus parasiticus and Aspergillus flavus1

1983 ◽  
Vol 46 (11) ◽  
pp. 940-942 ◽  
Author(s):  
LLOYD B. BULLERMAN
Keyword(s):  
Aspergillus Flavus ◽  
Yeast Extract ◽  
Aflatoxin B1 ◽  
Spore Germination ◽  
Mycelial Growth ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Potassium Sorbate ◽  
Mycelial Mass ◽  
Yeast Extract Sucrose

Growth and aflatoxin production by selected strains of Aspergillus parasiticus and Aspergillus flavus in the presence of potassium sorbate at 12°C were studied. Potassium sorbate at 0.05, 0.10 and 0.15% delayed or prevented spore germination and initiation of growth, and slowed growth of these organisms in yeast-extract sucrose broth at 12°C. Increasing concentrations of sorbate caused more variation in the amount of total mycelial growth and generally resulted in a decrease in total mycelial mass. Potassium sorbate also greatly reduced or prevented production of aflatoxin B1 by A. parasiticus and A. flavus for up to 70 d at 12°C. At 0.10 and 0.15% of sorbate, aflatoxin production was essentially eliminated. A 0.05% sorbate, aflatoxin production was greatly decreased in A. flavus over the control, but only slightly decreased in A. parasiticus.

Effect of ochratoxin A on aflatoxin production by Aspergillus parasiticus

1991 ◽  
Vol 69 (1) ◽  
pp. 16-17 ◽  
Author(s):  
M. Serafini ◽  
S. Foddai ◽  
S. Pieretti ◽  
L. Tomassini ◽  
M. Nicoletti
Keyword(s):  
Key Words ◽  
Ochratoxin A ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
High Concentrations ◽  
Key Words Aspergillus ◽  
Stimulation Of

The effect of ochratoxin A on growth and aflatoxin production by Aspergillus parasiticus was investigated. High concentrations had a significant effect, causing stimulation of aflatoxin production. Key words: Aspergillus parasiticus, aflatoxin production, ochratoxin A.

scholarly journals Antifungal Activity and Aflatoxin Degradation of Bifidobacterium Bifidum and Lactobacillus Fermentum Against Toxigenic Aspergillus Parasiticus

2016 ◽  
Vol 10 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Roshanak Daie Ghazvini ◽  
Ebrahim Kouhsari ◽  
Ensieh Zibafar ◽  
Seyed Jamal Hashemi ◽  
Abolfazl Amini ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Hplc Method ◽  
Bifidobacterium Bifidum ◽  
Lactobacillus Fermentum ◽  
Inhibition Rate ◽  
Plate Method ◽  
Mycelial Mass ◽  
Yeast Extract Sucrose

Food and feedstuff contamination with aflatoxins (AFTs) is a serious health problem for humans and animals, especially in developing countries. The present study evaluated antifungal activities of two lactic acid bacteria (LAB) against growth and aflatoxin production of toxigenic Aspergillus parasiticus. The mycelial growth inhibition rate of A. parasiticus PTCC 5286 was investigated in the presence of Bifidobacterium bifidum PTCC 1644 and Lactobacillus fermentum PTCC 1744 by the pour plate method. After seven days incubation in yeast extract sucrose broth at 30°C, the mycelial mass was weighed after drying. The inhibitory activity of LAB metabolites against aflatoxin production by A. parasiticus was evaluated using HPLC method. B. bifidum and L. fermentum significantly reduced aflatoxin production and growth rate of A. parasiticus in comparison with the controls (p≤0.05). LAB reduced total aflatoxins and B1, B2, G1 and G2 fractions by more than 99%. Moreover, LAB metabolites reduced the level of standard AFB1, B2, G1 and G2 from 88.8% to 99.8% (p≤0.05). Based on these findings, B. bifidum and L. fermentum are recommended as suitable biocontrol agents against the growth and aflatoxin production by aflatoxigenic Aspergillus species.

Growth, Sporulation and Aflatoxin Production by Aspergillus parasiticus on Strained Baby Foods

1980 ◽  
Vol 43 (6) ◽  
pp. 428-430 ◽  
Author(s):  
G. C. LLEWELLYN ◽  
N. E. DUCKHARDT ◽  
M. F. FISHER ◽  
T. EADIE ◽  
C. E. O'REAR
Keyword(s):  
Aspergillus Parasiticus ◽  
Total Yield ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Green Beans ◽  
Total Aflatoxin ◽  
Series Of Experiments ◽  
The Mean ◽  
Lower Temperature ◽  
Sporulation Rate

The potential for aflatoxin production by Aspergillus parasiticus on strained baby food was evaluated. Four puréed foods were inoculated with the mold and cultured at 15 and 26 C in two series of experiments. The aflatoxigenic mold produced mycelia and sporulated at both temperatures. The foods ranked in mean total yield of aflatoxin (μg/g of substrate) in the following order: peas > squash > green beans > pears. The ranking held consistent for both temperatures. Aflatoxins B1 and G1 were produced in higher percentages than B2 and G2 in each food at both temperatures. At 26 C, total aflatoxin produced ranged from 8 to 71 μg/g of substrate, and at 15 C, the mean for the four foods was from 3 to 50 μg/g of substrate. Temperature and substrate were the primary variables which contributed to sporulation rate, toxin production and toxin ratios. Peas and squash should be considered primary and highly supportive substrates for aflatoxin production if conditions should arise for spores to contaminate the products either during or after processing. Absolute prevention of aflatoxigenic spore contamination in these foods studied is essential. An occasional testing of these foods for aflatoxin seems warranted. A lower temperature during aflatoxin formation decreased the total toxin formed, but did not prohibit aflatoxin occurrence. A lower temperature also tended to divert the type of toxin produced from B1 to the less dangerous G1 and G2. Aflatoxin would appear to be a problem in these foods only under rare and unusual circumstances in relation to processing and consumer usage. If such aflatoxigenic spore contamination should occur, the levels produced would be significant.

Production of Aflatoxins B and G on Solid and Broth Culture Media

1977 ◽  
Vol 40 (12) ◽  
pp. 828-830 ◽  
Author(s):  
A. J. DELUCCA ◽  
R. Y. MAYNE ◽  
A. O. FRANZ ◽  
R. L. ORY
Keyword(s):  
Mycelial Growth ◽  
Aspergillus Parasiticus ◽  
Culture Media ◽  
Aflatoxin Production ◽  
The Other ◽  
Broth Culture ◽  
Solid Media

Mycelial growth and production of aflatoxins by Aspergillus parasiticus NRRL A-16,462, on whole peanuts, undelintered cottonseed, and shredded wheat was compared to growth and aflatoxin production on 10% broths of the same substrates. The greatest amount of toxins was produced on shredded wheat as a whole substrate. The least amount was produced in shredded wheat broth medium. Intermediate levels of toxin were produced on cottonseed in both types of media. More aflatoxin was produced on peanut broth than on whole peanuts. Mycelial growth on whole shredded wheat was greater than growth on the other solid media though it had the lowest amount of growth of the three broth media. Among whole substrates, growth on fuzzy cottonseed was least but mycelial growth on cottonseed broth was second of the three broth media. Growth on whole peanuts was as great as on shredded wheat; on peanut broth, it was the greatest of the three broth media. On whole substrates, the ratio of aflatoxins B:G produced was highest on shredded wheat and lowest on peanuts. In broth culture the B:G ratio was largest on the cottonseed and lowest on shredded wheat.

Aflatoxin Production by Aspergillus parasiticus and Its Stability During the Manufacture of Korean Soy Paste (Doenjang) and Soy Sauce (Kanjang) by Traditional Method

1988 ◽  
Vol 51 (12) ◽  
pp. 938-944 ◽  
Author(s):  
KUN-YOUNG PARK ◽  
KYU-BOK LEE ◽  
LLOYD B. BULLERMAN
Keyword(s):  
Bacillus Subtilis ◽  
Mixed Culture ◽  
Traditional Method ◽  
Degradation Rate ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Soy Sauce ◽  
Natural Conditions ◽  
Soybean Cake

Aflatoxin (AF) production and its stability on meju (crushed Korean soybean cake) by a pure culture of Aspergillus parasiticus and a mixed culture of A. parasiticus. A. oryzae and Bacillus subtilis (fermentation 1) during the manufacture by traditional methods of Korean soy paste (doenjang) and soy sauce (kanjang) were studied. There was a difference in the amount of aflatoxin production on different varieties of soybeans, but the pattern of toxin production was similar. During fermentation, more total aflatoxins were produced under the mixed culture condition. Aflatoxin G1 (AFG1) production was highly stimulated though it degraded quickly, whereas aflatoxin B1 (AFB1) synthesis was low. The exposure of the meju to sunlight during fermentation had no effect in reducing aflatoxin synthesis. When the meju fermented under natural conditions (fermentation 2) with a contamination by A. parasiticus, high levels of aflatoxins were still produced. After a month of ripening of the fermented meju with charcoal in brine, more AFG1 than AFB1 was degraded in both the pure and mixed culture samples (degradation %; B1:2–69%, G1:31–84%). When the meju was ripened in water, most of the aflatoxins were degraded (B1:95–99%, G1:100%) in a month, accompanied by a significant increase in pH (p<0.05). A greater amount of aflatoxins (96–100%) was detected in the meju when it was ripened in brine, however, lower amounts (25–85%) of aflatoxins remained in meju ripened in water. During three months of ripening in brine, 83–98% of AFB1 and 98–100% of AFG1 were degraded in fermentation 1, but the degradation rate was slower (B1 :63%, G1:98%) following fermentation 2. The total levels of aflatoxins remaining were significantly (p<0.05) reduced when charcoal was added to the mixture.

scholarly journals Interaction Between Certain Moulds and Aflatoxin B1 Producer Aspergillus Flavus NRRL 3251

2007 ◽  
Vol 58 (4) ◽  
pp. 429-434 ◽  
Author(s):  
Zdenka Cvetnić ◽  
Stjepan Pepeljnjak
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Airborne Fungi ◽  
Biotic Interaction ◽  
Detectable Amount ◽  
Single Culture ◽  
Aflatoxin B ◽  
Yeast Extract Sucrose

Interaction Between Certain Moulds and Aflatoxin B1ProducerAspergillus FlavusNRRL 3251The objective of this study was to evaluate biotic interaction between some mould species and active producer of aflatoxin B1Aspergillus flavusNRRL 3251, co-cultured in yeast-extract sucrose (YES) broth. Twenty-five mould strains ofAlternariaspp.,Cladosporiumspp.,Mucorspp.,A. flavusandA. niger, used as biocompetitive agents, were isolated from outdoor and indoor airborne fungi, scrapings of mouldy household walls, and from stored and post-harvest maize. Aflatoxin B1was extracted from mould biomasses with chloroform and detected using the multitoxin TLC method. The results confirm antagonistic interaction between all strains tested. WithAlternariaspp. andCladosporiumspp., aflatoxin B1production decreased 100 %, compared to detection in a single culture ofA. flavusNRRL 3251 (Cmean=18.7 μg mL-1). In mixed cultures withMucorspp., aflatoxinB1levels dropped to (5.6-9.3) μg mL-1, and the inhibition was from 50 % to 70 %. Four of five aflatoxin non-producing strains ofA. flavusinterfered with aflatoxin production in mixed culture, and reduced AFB1productivity by 100 %. One strain showed a lower efficacy in inhibiting AFB1production (80 %) with a detectable amount of AFB13.7 μg mL-1when compared to control. A decrease in toxin production was also observed in dual cultivation withA. nigerstrains. It resulted in 100 % reduction in three strains), 90 % reduction in one strain (Cmean=1.9 μg mL-1) and 80 % reduction in one strain (Cmean=3.7 μg mL-1) inhibition.

Aspergillus parasiticus NRRL 2667 Growth and Aflatoxin Synthesis as Affected by Calcium Content and Initial Spore Load in Single Peanuts

1994 ◽  
Vol 57 (5) ◽  
pp. 415-418 ◽  
Author(s):  
MA. ROCELLE S. CLAVERO ◽  
MARK A. HARRISON ◽  
YEN-CON HUNG
Keyword(s):  
Mycelial Growth ◽  
Aspergillus Parasiticus ◽  
Calcium Supplementation ◽  
Calcium Content ◽  
Maximum Growth ◽  
Aflatoxin Production ◽  
Toxin Production ◽  
Plate Counts ◽  
Layer Chromatography ◽  
Spore Load

Mycelial growth of Aspergillus parasiticus NRRL 2667 and aflatoxin production on Florunner peanuts grown under different calcium supplementation levels (CSL) (550, 1,100, 2,200 and 4,400 kg gypsum/ha) with initial spore loads of 102, 104 and 106 spores/g were investigated. Growth at 25°C for 0, 4, 7 and 14 days was determined by viable plate counts on Aspergillus flavus/parasiticus agar (AFPA) medium. Irrespective of the initial spore load, maximum growth of 108 to 109 CFU/g was attained after 14 days except for the 4,400 kg/ha Ca-supplemented nuts on which the maximum populations were one log less. Aflatoxins B1 and G1 concentrations measured by thin layer chromatography (TLC) ranged from 0–3460 (μg/g and 0–3740 (μg/g, respectively. Toxin production was highest in single peanuts with CSL of 2,200 kg/ha. A reduction of 50% or higher was observed as CSL was increased to 4,400 kg/ha.

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