Depletion of Sorbate from Different Media During Growth of Penicillium Species

1982 ◽  
Vol 45 (5) ◽  
pp. 398-404 ◽  
Author(s):  
MARIA L. FINOL ◽  
ELMER H. MARTH ◽  
ROBERT C. LINDSAY
Keyword(s):  
Paper Chromatography ◽  
Inhibitory Action ◽  
The Other ◽  
Penicillium Roqueforti ◽  
Species Concentration ◽  
Penicillium Species ◽  
Initial Concentration ◽  
Penicillium Crustosum ◽  
Penicillium Cyclopium ◽  
Penicillium Viridicatum

Penicillium cyclopium, Penicillium roqueforti, Penicillium viridicatum, Penicillium puberulum, Penicillium cyclopium (atypical strain), Penicillium crustosum and Penicillium lanoso-viride were isolated from spoiled cheese. These molds grew and depleted sorbate from media when the chemical was present initially at a concentration of up to 3,000, 10,000, 6,000, 12,000, 12,000, 7,000 and 3,000 ppm, respectively. A combination of paper chromatography and spectrophotometry was used to determine amounts of residual sorbate. Seventy-one to 100% of sorbate present initially was depleted from media by the various molds during 4–20 days of incubation at 21°C and 22–48 days at 4°C. The substrate influenced growth of mold and depletion of sorbate, but uniform behavior was not observed for all the Penicillium species studied. For example, presence of 3,000 ppm of sorbate plus 1% casein in the medium inhibited P. cyclopium and P. lanoso-viride but not the other five species. Concentration of sorbate (3,000 – 9,000 ppm) plus temperature (4, 12, 21 °C) were important for inhibitory action of the preservative on P. cyclopium, P. viridicatum, P. crustosum and P. lanoso-viride but not P. puberulum, P. cyclopium (atypical strain) which grew at 4 °C and depleted sorbate when the initial concentration was up to 9,000 ppm and P. roqueforti which grew at up to 6,000 ppm at the same temperature.

Proteolytic and Lipolytic Activities of some Toxigenic and Nontoxigenic Aspergilli and Penicillia

1980 ◽  
Vol 43 (5) ◽  
pp. 354-355 ◽  
Author(s):  
S. M. EL-GENDY ◽  
E. H. MARTH
Keyword(s):  
Proteolytic Activity ◽  
Aspergillus Flavus ◽  
Aspergillus Parasiticus ◽  
Lipolytic Activity ◽  
The Other ◽  
Aspergillus Ochraceus ◽  
Penicillium Roqueforti ◽  
Two Cultures ◽  
Penicillium Cyclopium ◽  
Penicillium Patulum

Eighteen strains of Aspergillus flavus or Aspergillus parasiticus, one of Aspergillus ochraceus and 12 strains or species of Penicillium, many of them isolated from cheese, were evaluated for their proteolytic and lipolytic activities. Strains of A. flavus exhibited considerable proteolytic and little lipolytic activity, whereas the reverse was true for strains of A. parasiticus. Of the Penicillium cultures tested, 10 exhibited considerable lipolytic activity, but only five had marked proteolytic activity. Two cultures, Penicillium patulum M59, and Penicillium cyclopium No. 8, were markedly lipolytic and proteolytic. Of the other cultures, greatest lipolytic activity was associated with Penicillium roqueforti 849, Penicillium puberulum No. 33, A. parasiticus NRRL 3145 and NRRL 465 and A. ochraceus NRRL 3174, whereas greatest proteolytic activity of all the cultures was associated with P. patulum M59, P. cyclopium No. 25 and A. flavus WB500, 4018, 4098 and NRRL 5565.

Differentiation of Penicillium roqueforti from Closely Related Species Contaminating Cheeses and Dairy Environment

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 222
Author(s):  
Miloslava Kavková ◽  
Jaromír Cihlář ◽  
Vladimír Dráb ◽  
Ladislav Bár
Keyword(s):  
Related Species ◽  
Morphological Variability ◽  
The Other ◽  
Morphological Properties ◽  
Penicillium Roqueforti ◽  
Molecular Tools ◽  
Penicillium Species ◽  
Culture Collections ◽  
Closely Related Species

Currently, Penicillium roqueforti and the closely related P. carneum and P. paneum are identified based on their macromorphology, micromorphology, and molecular properties, the determination of which involves time-consuming procedures. Culture collections focused on dairy isolates of P. roqueforti require quick and efficient tools for routine applications to identify the (a) taxonomy affiliation and (b) morphological properties of strains that influence the sensory properties of blue-veined cheeses. Here, we assessed the morphological variability of P. roqueforti, P. carneum, P. paneum, and P.crustosum on artificial, Edam-like, and Roquefort-like media. Molecular tools were used to test P. roqueforti strains and clones effectively. A novel primer, PrsF, was tested for specificity within strains and isolates of P. roqueforti compared to P. carneum, P. paneum, and P. crustosum. The results reveal that PrsF was specific to the P. roqueforti samples and did not amplify the other tested Penicillium species. Identification based simultaneously on the specificity of the PrsF primer pair and cultivation of P. roqueforti strains on Roquefort-like medium represents an effective method for expanding the collections and practical use of P. roqueforti in the dairy industry.

Total lactate dehydrogenase and its isoenzymes in serum in the presence of penicillamine and other sulfhydryl compounds.

1977 ◽  
Vol 23 (2) ◽  
pp. 229-233 ◽  
Author(s):  
L L Gershbein ◽  
K G Raikoff
Keyword(s):  
Lactate Dehydrogenase ◽  
Disc Electrophoresis ◽  
The Other ◽  
Rat Serum ◽  
Initial Concentration ◽  
Ldh Isoenzymes ◽  
Allosteric Effectors ◽  
Sulfhydryl Compounds

Abstract Toward delineation of changes in total lactate dehydrogenase (LDH) and in the distribution of LDH isoenzymes as assessed by polyacrylamide disc electrophoresis, we inbucated human and rat sera with various agents, notably sulfhydryl compounds. Although artefacts were apparent when these agents were used without preliminary adjustment of pH, we saw little alteration in total unitage when one or two volumes of serum was mixed with one volume of any of several thiols, especially penicillamine, at an initial concentration of 0.4 mol/liter and pH 7.0-7.5. Under these conditions, penicillamine caused a loss in LDH-5 after incubation for 1 h at 25 degrees C together with small decreases in mobility of the other four isoenzymes toward the anode. A zymosan region appeared below the albumin and tracking dye area. With longer periods of incubation of rat serum with penicillamine or alpha-mercaptosuccinate, a novel band in the zymogram was noted just above the LDH-4 peak. The observations are discussed in terms of allosteric effectors.

Roquefortine C Occurrence in Blue Cheese

2001 ◽  
Vol 64 (2) ◽  
pp. 246-251 ◽  
Author(s):  
CARLO FINOLI ◽  
ANGELA VECCHIO ◽  
ANTONIETTA GALLI ◽  
IVAN DRAGONI
Keyword(s):  
Yeast Extract ◽  
Culture Media ◽  
Penicillium Roqueforti ◽  
Blue Cheese ◽  
Penicillium Crustosum ◽  
Sucrose Medium ◽  
Low Toxicity ◽  
Yeast Extract Sucrose ◽  
Roquefortine C

Several strains of Penicillium are used for the production of mold-ripened cheeses, and some of them are able to produce mycotoxins. The aims of the research were the determination of roquefortine C and PR toxin in domestic and imported blue cheeses, the identification of the penicillia used as starter, and the investigation of their capacity for producing toxins in culture media. Roquefortine C was always found in the cheeses at levels ranging from 0.05 to 1.47 mg/kg, whereas the PR toxin was never found. The identification of the fungal strains present in the domestic cheeses included Penicillium glabrum, Penicillium roqueforti, and Penicillium cyclopium in the Gorgonzola “dolce” and Penicillium roqueforti in the Gorgonzola “naturale”; in one case, the presence of Penicillium crustosum was observed. The strains isolated from the foreign cheeses belonged to P. roqueforti. The strains were able to produce between 0.18 and 8.44 mg/liter of roquefortine in yeast extract sucrose medium and between 0.06 and 3.08 mg/liter and less than 0.05 mg/liter when inoculated in milk at 20°C for 14 days and 4°C for 24 days, respectively. Linear relations between production of roquefortine in culture media and cheeses did not emerge. PR toxin ranged from less than 0.05 to 60.30 mg/liter in yeast extract sucrose medium and was produced in milk at 20°C from only one strain. The low levels and the relatively low toxicity of roquefortine make the consumption of blue cheese safe for the consumer.

Prolactin inhibits corticoid-induced differentiation of active Na+ transport across cultured frog tadpole skin

1995 ◽  
Vol 269 (5) ◽  
pp. C1326-C1331 ◽  
Author(s):  
M. Takada ◽  
H. Yai ◽  
K. Takayama-Arita
Keyword(s):  
Acetylcholine Receptor ◽  
Inhibitory Action ◽  
Blood Group Antigen ◽  
The Other ◽  
Basal Cells ◽  
Na Transport ◽  
Adult Type ◽  
Frog Tadpole ◽  
Antigen A ◽  
Tadpole Skin

Active Na+ transport differentiates in larval bullfrog skin cultured with corticoids. After 2 wk in culture, the epidermis became positive against human blood group antigen A, the marker for the adult-type cells of the epidermis, but was negative to the antibody against the acetylcholine receptor, the marker for the larval-type epidermis. Amiloride (10(-5) M) did not inhibit the differentiation of active Na+ transport. On the other hand, in skin cultured with prolactin (2 micrograms/ml), the epidermis remained negative against antigen A and positive against acetylcholine receptor, and the differentiation of active Na+ transport was inhibited. Thyroid hormone did not antagonize the inhibitory action of prolactin on this transport differentiation. Prolactin affected the basal cells of the larval epidermis and inhibited development of corticoid-induced adult features in the epidermis.

COMPARISON OF THE ANTI-FERTILITY AND SEX HORMONAL ACTIVITIES OF SEX HORMONES AND THEIR DERIVATIVES

1964 ◽  
Vol 47 (3) ◽  
pp. 444-456 ◽  
Author(s):  
P. A. Desaulles ◽  
C. Krähenbühl
Keyword(s):  
Sex Hormones ◽  
Relative Intensity ◽  
Sex Steroids ◽  
Inhibitory Action ◽  
Inhibitory Effect ◽  
The Other ◽  
Potent Effect ◽  
Other Hand ◽  
Activity Ratios ◽  
Blastocyst Implantation

ABSTRACT The following effects exerted by sex steroids were measured: androgenic, oestrogenic, and progestational effects; the inhibition action on gonadotrophic function in castrated animals; and the inhibitory action on ovulation and blastocyst implantation in intact animals. The steroids investigated consisted of oestradiol and its ethinyl and methoxyethinyl derivatives, oestrone, oestriol, testosterone, progesterone, and certain synthetic gestagens derived from nortestosterone, i. e. norethisterone, norethynodrel, and ethinyl-oestrenol. Oestradiol and its derivatives show potent anti-gonadotrophic activity, a relatively less potent anti-ovulatory effect, and a marked inhibitory action on implantation. Progesterone, on the other hand, which has only a very weak inhibitory effect on gonadotrophic function, shows an anti-ovulatory activity which, though inferior in absolute terms to that of oestradiol, is still appreciable, whereas it exerts almost no effect at all on implantation. With regard to its activity ratios, testosterone occupies a position mid-way between the two female hormones. Norethisterone, norethynodrel, and ethinyl-oestrenol have a more potent effect on gonadotrophic function than progesterone. The activity ratios for the effects of these three compounds on ovulation and implantation are comparable to those of oestradiol or progesterone, depending on the relative intensity of their oestrogenic and progestational action. The nature of the endocrine changes produced by these compounds are discussed with reference to the inhibition of ovulation and implantation.

scholarly journals ANTIGENIC COMPETITION BETWEEN POLYPEPTIDYL DETERMINANTS IN NORMAL AND TOLERANT RABBITS

1968 ◽  
Vol 127 (2) ◽  
pp. 237-250 ◽  
Author(s):  
Israel Schechter
Keyword(s):  
Immune Response ◽  
Antibody Formation ◽  
Inhibitory Action ◽  
The Other ◽  
Protein Carrier ◽  
Protein Conjugate ◽  
Considerable Degradation ◽  
Antigenic Competition

Competition between two polypeptidyl determinants was studied in normal rabbits and rabbits made tolerant to the competing antigen. The capacity of poly-DL-phenylalanyl protein conjugate to inhibit the formation of antibodies specific to the poly-DL-alanyl determinant was dependent on the nature of the protein carrier of the singly substituted antigens. Competition occurred only when the peptidyl determinants were attached to identical or similar (RSA and HSA) carriers. Thus, the immune response toward the poly-DL-alanyl determinant was impaired by injecting the pairs p-DL-PheRSA and p-DL-AlaHSA, or p-DL-PheRNase and p-DL-AlaRNase. Suppression of the formation of antibodies with poly-DL-alanyl specificity was not observed, however, upon administration of p-DL-PheRSA together with p-DL-AlaRNase or of p-DL-PheRNase with p-DL-AlaHSA. Tolerance to p-DL-PheRSA was induced by injecting this material into newborn rabbits. The tolerant animals retained their capacity to produce anti-poly-DL-alanyl antibodies upon injection of p-DL-AlaRSA or p-DL-AlaHSA. However, when these poly-DL-alanyl proteins were administered together with p-DL-PheRSA, antibodies against the poly-DL-alanyl determinant were not formed even though no antibodies with poly-DL-phenylalanyl specificity were produced. These results indicate that in competition experiments the preference in the immune response against a given determinant is dependent not only on the nature of the competing determinants, but it is also governed to a large extent by the over-all properties of the antigenic molecules. This suggests that at the stage at which the competition occurs the competing molecules had not undergone considerable degradation. On the basis of experiments with tolerant animals, it is suggested that in normal animals antibody formation to the competing antigen is not the cause of its inhibitory action on the response against the other antigen. The competition experiments described suggest that an antibody-forming cell is multipotent.

Antifungal Activity of the Essential Oil of Origanum syriacum L.

1995 ◽  
Vol 58 (10) ◽  
pp. 1147-1149 ◽  
Author(s):  
RASHA K. DAOUK ◽  
SHAWKY M. DAGHER ◽  
ELSA J. SATTOUT
Keyword(s):  
Liquid Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Inhibitory Action ◽  
Inhibitory Concentration ◽  
Volatile Oil ◽  
Gas Liquid Chromatography ◽  
Penicillium Species ◽  
Origanum Oil ◽  
Yeast Extract Sucrose

The volatile oil of the Lebanese Za'atar (Origanum syriacum L.) was characterized for its thymol and carvacrol content using gas-liquid chromatography. These two compounds constituted the major components of the oil and were present in equal proportions of 30% in the volatile oil extracted from the leaves and shoot tips of the Origanum plant during the preflowering stage. The percentage of carvacrol in the essential oil increased to 62% after flowering and maturation, while the concentration of thymol decreased to 14%. Origanum oil extracted from plants collected during midseason was evaluated for its antifungal activity against Aspergillus niger, Fusarium oxysporum, and Penicillium species. The oil exhibited strong inhibitory action against the three fungi tested. The minimum inhibitory concentration (MIC) of the oil was found to be 0.1 μl/ml of yeast extract sucrose broth for the fungi tested.

Nervous Control of Gut Movements in Lophius

1980 ◽  
Vol 60 (1) ◽  
pp. 19-30 ◽  
Author(s):  
J. Z. Young
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Inhibitory Action ◽  
Sympathetic Nerves ◽  
The Other ◽  
Sufficient Data ◽  
Nervous Control ◽  
Autonomic Nervous ◽  
Hand Control ◽  
Excitatory Action

There are not sufficient data available to allow any general statements about the earlier stages of evolution of the autonomic nervous system and of its various transmitter mechanisms. In the previous paper (Young, 1980) it was shown that control of the stomach of elasmobranchs is largely by the inhibitory action of the sympathetic nerves, probably mediated by 5-HT. In teleostean fishes on the other hand control seems to be mainly by the cholinergic excitatory action of the vagus, especially in the more advanced (acanthopterygian) groups (Grove & Campbell, 1979a, b; Fänge & Grove, 1979).

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