DNA-Based Rapid Methods for the Detection of Foodborne Pathogens

1991 ◽  
Vol 54 (5) ◽  
pp. 387-401 ◽  
Author(s):  
MARK J. WOLCOTT
Keyword(s):  
Escherichia Coli ◽  
Yersinia Enterocolitica ◽  
Foodborne Pathogens ◽  
Food Microbiology ◽  
Detection Methods ◽  
Foodborne Illnesses ◽  
Salmonella Spp ◽  
Rapid Methods ◽  
Shigella Spp ◽  
Reliable Methods

The reports of foodborne illnesses are increasing and rapid, sensitive and reliable methods to detect and identify foodborne microorganisms are needed. This review encompasses a description of recent technologies upon which DNA detection methods are based. This review also provides current information on hybridization assays developed or under study for application in food microbiology. Literature pertaining to some of the most common foodborne microorganisms, including Salmonella spp., Shigella spp., Listeria spp., Escherichia coli, and Yersinia enterocolitica is emphasized.

scholarly journals Etiology of acute diarrhea in children in Shanghai, 2015-2018

2019 ◽  
Author(s):  
Hailing Chang ◽  
Jiayin Guo ◽  
Zhongqiu Wei ◽  
Zheng Huang ◽  
Chuning Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Acute Diarrhea ◽  
Enteric Bacteria ◽  
Enterohemorrhagic Escherichia Coli ◽  
Enteric Pathogens ◽  
Enterotoxigenic Escherichia Coli ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Fresh Stool

Abstract Background Diarrhea is still a major cause of childhood morbidity and mortality worldwide. This hospital-based study aimed to monitor the consecutive epidemiological trend of etiology in children with acute diarrhea in Shanghai.Methods Outpatient children with diarrhea were prospectively enrolled within 7 days after onset of diarrheal symptoms during 2015-2018. Fresh stool samples were collected for testing enteropathogens. Enteric bacteria were identified and typed through culture and serotyping. Enteric viruses were identified through real-time PCR assay. hadResults Enteric pathogens were identified in 1572 (58.4%) of the 2692 enrolled children with acute diarrhea. Viruses were more frequently detected than bacteria (41.3% versus 25.0%), and co-infection with 2 or more pathogens was found in 13% of outpatients. Nontyphoidal Salmonella spp . (NTS) was the most common bacteria with 10.3% of isolate rate, followed by enteropathogenic Escherichia coli (EPEC) (6.5%), enteroaggregative Escherichia coli (EAEC) (6.2%), Campylobacter spp . (3.6%), enterotoxigenic Escherichia coli (ETEC) (1.1%), Shigella spp . (0.2%), and enterohemorrhagic Escherichia coli (EHEC) (0.1%). Rotavirus was the most common virus with 16.0% of detection rate, followed by norovirus (15.5%), adenovirus (7.2%), sapovirus (3.0%) and astrovirus (2.7%).Conclusions Infectious diarrhea remains the major cause of diarrhea in children in Shanghai. Rotavirus, norovirus and NTS were the major enteric pathogens responsible for diarrhea in Shanghainese children. Improving uptake of rotavirus vaccine and strengthening prevention of foodborne pathogens will be helpful to reduce the burden of diarrheal diseases in children in Shanghai.

scholarly journals Hoàn thiện quy trình phát hiện đồng thời 14 vi khuẩn gây bệnh đường ruột bằng kĩ thuật PCR-Reverse Dot Blot (PCR-RDB)

2020 ◽  
Vol 13 (1) ◽  
pp. 66-80
Author(s):  
Hồ Thị Thanh Thủy ◽  
Nguyễn Văn Trường ◽  
Nguyễn Bảo Toàn ◽  
Lao Đức Thuận ◽  
Trương Kim Phượng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Campylobacter Jejuni ◽  
Yersinia Enterocolitica ◽  
Vibrio Parahaemolyticus ◽  
Clostridium Botulinum ◽  
Escherichia Coli O157 ◽  
Dot Blot ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Reverse Dot Blot

Ngộ độc thực phẩm, với một trong những nguyên nhân chính do nhiễm khuẩn vẫn luôn là mối lo ngại, mang tính toàn cầu, được Tổ chức Sức khỏe Thế giới rất quan tâm. Việc xác định chính xác đối tượng vi khuẩn nhiễm vẫn luôn là một nhu cầu cấp thiết của các labo lâm sàng. Nghiên cứu trước đây của chúng tôi đã được công bố với việc thành công bước đầu trong việc xây dựng một quy trình dựa trên kĩ thuật PCR-Reverse Dot Blot (PCR-RDB) nhằm xác định đồng thời 12 vi khuẩn gây bệnh đường ruột ch yếu, bao gồm Bacillus cereus, Clostridium botulinum, Clostridium perfringen, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, Salmonella spp., Shigella spp.., Vibrio cholerae, Vibrio parahaemolyticus, Yersinia enterocolitica và Brucella spp. Chúng tôi tiếp tục phát triển nghiên cứu này nhằm hoàn thiện quy trình PCR-RDB bằng việc thiết kế bổ sung trên màng các loại mẫu dò nhằm làm chứng dương, chứng âm, chứng màu và chứng kiểm tra tín hiệu nền. Bên cạnh đó, xét nhu cầu lâm sàng, việc bổ sung hai loại mẫu dò đề dò hai vi khuẩn Campylobacter jejuni và Yersinia enterocolitica O:8 gây bệnh đường ruột chủ yếu ở trẻ em, là cần thiết. Quy trình PCR-RDB được hoàn thiện trong nghiên cứu này vì vậy có khả năng phát hiện đồng thời 14 vi khuẩn gây bệnh đường ruột, một cách đặc hiệu, với độ nhạy đạt 10^2 bản sao/ml, đã được thử nghiệm trên 30 mẫu phân, ghi nhận kết quả hoàn toàn khớp với kít thương mại PowerCheckTM 20 Pathogen Multiplex Real-time PCR Kit (Korea).

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

scholarly journals Occurrence and Levels of Salmonella, Enterohemorrhagic Escherichia coli, and Listeria in Raw Wheat

2019 ◽  
Vol 82 (6) ◽  
pp. 1022-1027 ◽  
Author(s):  
SAMUEL P. MYODA ◽  
STEFANIE GILBRETH ◽  
DEANN AKINS-LEWENTHAL ◽  
SEANA K. DAVIDSON ◽  
MANSOUR SAMADPOUR
Keyword(s):  
Escherichia Coli ◽  
Wheat Flour ◽  
Foodborne Illness ◽  
Enterohemorrhagic Escherichia Coli ◽  
Microbial Pathogens ◽  
Foodborne Illnesses ◽  
Salmonella Spp ◽  
Naturally Occurring ◽  
Specific Pathogen ◽  
Wheat Products

ABSTRACT Wheat flour has been implicated in several outbreaks of foodborne illness in recent years, yet little information is available regarding microbial pathogens in wheat and wheat flour. Information about microbial pathogens in wheat is needed to develop effective methods to prevent foodborne illnesses caused by wheat products. From 2012 to 2014, we conducted a baseline study to determine the prevalence and levels of pathogens in wheat samples taken before milling. A total of 5,176 wheat samples were tested for enterohemorrhagic Escherichia coli (EHEC), Salmonella spp., Listeria spp., and L. monocytogenes. Positive samples were assayed for most probable numbers (MPNs), and isolates were fingerprinted by pulsed-field gel electrophoresis (PFGE). The rate of detection of each pathogen tested was as follows: Salmonella was in 1.23% of the samples (average level of 0.110 MPN/g), EHECs occurred in 0.44% of the samples (0.039 MPN/g), and Listeria spp. occurred in 0.08% of samples (0.020 MPN/g), but L. monocytogenes was not detected. The PFGE assessment found a high diversity for all organisms. All EHEC PFGE patterns (22 of 22) were unique, and 39 of 47 Salmonella patterns (83%) were unique. These results indicate a diverse background of naturally occurring organisms. These findings suggest that the microbial contamination is coming from diverse sources and provide no evidence in support of a specific pathogen load. Altogether, our surveillance study shows that contamination of wheat with pathogens is clearly evident and poses a foodborne illness risk. HIGHLIGHTS

scholarly journals Avaliação de Procedimentos Operacionais Padronizados Implementados na Produção e Manipulação de Fórmulas Infantis em Hospital Federal

2021 ◽  
Vol 25 (2) ◽  
pp. 128-135
Author(s):  
Mariana Fernandes Garcia da Silva ◽  
Maria das Graças Gomes de Medeiros ◽  
Robson Maia Franco ◽  
Maria Carmela Kasnowski Holanda Duarte ◽  
Sergio Girão Barroso
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Yersinia Enterocolitica ◽  
Infant Formulas ◽  
Salmonella Spp ◽  
Total Coliforms ◽  
Mesophilic Bacteria ◽  
Enterococcus Spp ◽  
Standard Operating

A contaminação de fórmulas infantis pode ocorrer quando os manipuladores cometem falhas operacionais ou falhas na adequação do ambiente de preparo. O objetivo da pesquisa foi avaliar a eficácia dos procedimentos operacionais padronizados executados na área de produção de fórmulas lácteas de um Hospital. Realizou-se uma pesquisa aplicada, descritiva e explicativa com avaliação do fluxo operacional e das condições físico funcionais, análise do comportamento dos manipuladores e pesquisas microbiológicas de Coliformes totais, Escherichia coli, Bactérias aeróbias mesófilas, Bacillus cereus, Yersinia enterocolitica, Listeria monicytogenes, Clostridium perfrigens, Clostridium Sulfito Redutor, Staphylococcus coagulase positiva, Salmonella spp., Enterococcous spp, Enterobacter sakazakii, Pseudomonas aeruginosa e fungos em superfícies de fórmulas infantis e água. Os procedimentos operacionais padronizados são executados em todas as etapas do processo, entretanto, foram observadas falhas humanas. Na avaliação físico-uncional, 92% estavam em conformidade; na pesquisa microbiológica de superfícies, 12% de resultados deram presenças para Enterococcus spp., Coliformes totais, Escherichia coli e Bactérias heterotróficas aeróbias mesófilas. Nas fórmulas infantis, 8,33% dos resultados mostraram presença para Coliformes totais, Bactérias heterotróficas aeróbias mesófilas e Fungos. Os procedimentos implantados asseguram a qualidade sanitária das fórmulas, mas se verificou a necessidade de treinamento dos manipuladores. Palavras-chave: Doenças Transmitidas por Alimentos. Fórmulas Infantis. Segurança Alimentar e Nutricional. Risco Sanitário. Abstract Infant formulas contamination may occur when handlers commit operational failures or failures in the environment adequacy. The objective of this research was evaluate the effectiveness of standard operating procedures performed in the production area of milk formulations of a Hospital. An applied, descriptive and explanatory research was conducted with evaluation of operational flow and physical functional conditions, analysis of the manipulators’s behavior and microbiological investigations of MPN of total Coliforms, Escherichia col and Enterococcus spp., Count of Bacillus cereus/g, Clostridium sulfite reducer, molds and yeasts, Staphylococcus coagulase positive, aerobic mesophilic bacteria and Cronobacter sakazakii, research of Yersinia enterocolitica, Listeria monocytogenes, Salmonella spp and Pseudomonas aeruginosa on surfaces, infant formulas and water. Standard operating procedures were performed at all the process stages, however, human failures have been observed. In the physical functional evaluation, 92% was in compliance; on the surface microbiological analysis, 12% of the results present Enterococcus spp., Total Coliforms, Escherichia coli and aerobic mesophilic bacteria. In infant formulas, 8.33% results showed presence for Total Coliforms, aerobic mesophilic bacteria and Fungi. The implanted procedures assured the formulas sanitary quality, but the necessity of the manipulators’s training was verified. Keywords: Foodborne Diseases. Infant Formulas. Food and Nutrition Security. Health Risk.

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