Evaluation of Combination Treatment Processes for the Microbial Decontamination of Pork Trim†

2001 ◽  
Vol 64 (3) ◽  
pp. 335-342 ◽  
Author(s):  
MAURICIO M. CASTELO ◽  
DONG-HYUN KANG ◽  
GREGORY R. SIRAGUSA ◽  
MOHAMMAD KOOHMARAIE ◽  
ELAINE D. BERRY
Keyword(s):  
Lactic Acid ◽  
Storage Period ◽  
Hot Water ◽  
Microbial Populations ◽  
Aerobic Bacteria ◽  
Psychrotrophic Bacteria ◽  
C Storage ◽  
Combination Treatments ◽  
Treatment Processes ◽  
Hot Air

Combination treatment processes for the microbial decontamination of pork trim were developed and evaluated. Lean pork trim tissue (LPT) and fat-covered pork trim tissue (FPT) inoculated with swine feces were treated with intervention processes as follows: (i) control (untreated), (ii) water (15°C, 120 s), (iii) water followed by lactic acid wash (15°C, 75 s), (iv) combination 1 (water plus hot water [65.5°C, 15 s] plus hot air [510°C, 60 s] plus lactic acid), (v) combination 2 (water plus hot water [82.2°C, 15 s] plus hot air [510°C, 75 s] plus lactic acid), and (vi) combination 3 (water plus hot water [82.2°C, 45 s] plus hot air [510°C, 90 s] plus lactic acid). Populations of aerobic bacteria, psychrotrophic bacteria, coliforms, Escherichia coli, and lactic acid bacteria were determined before and after treatment and at days 2 and 7 of 4°C storage. Regardless of the intervention treatment, lower microbial populations were observed on FPT than on LPT immediately after treatment and during the 7-day storage period. Both LPT and FPT treated with water plus lactic acid, combination 1, combination 2, and combination 3 had lower remaining populations of all microbial groups immediately after treatment than did water-treated samples. Populations of aerobic bacteria, coliforms, E. coli, and lactic acid bacteria on either LPT or FPT did not statistically increase during the 7-day storage period. On LPT, populations of psychrotrophicbacteria grew during 4°C storage but remained lower at day 7 on LPT treated by combinations 2 and 3 (2.29 and 1.89 log10 CFU/cm2, respectively) than on LPT treated with water (4.07 log10 CFU/cm2) or water plus lactic acid (3.52 log10 CFU/cm2). Populations of psychrotrophic bacteria remained below detectable levels throughout the 7-day storage on FPT treated with water plus lactic acid or any of the three combination treatments. Treatment of pork trim with any of the combination treatments significantly (P < 0.05) affected the color and emulsion stability of the ground pork. Water and water plus lactic acid were the most favorable treatments in reducing microbial populations on pork trim without affecting the quality attributes of the ground pork.

Microbial and Quality Attributes of Ground Pork Prepared from Commercial Pork Trim Treated with Combination Intervention Processes†

2001 ◽  
Vol 64 (12) ◽  
pp. 1981-1987 ◽  
Author(s):  
MAURICIO M. CASTELO ◽  
MOHAMMAD KOOHMARAIE ◽  
ELAINE D. BERRY
Keyword(s):  
Lactic Acid ◽  
Emulsion Stability ◽  
Quality Attributes ◽  
Microbial Reduction ◽  
Aerobic Bacteria ◽  
Negative Effects ◽  
Treatment Processes ◽  
Hot Air ◽  
Ground Pork ◽  
Acid Wash

The effects of combination intervention treatments of commercial pork trim on microbial and quality attributes of the subsequent ground pork were examined. Fresh commercial pork trim was inoculated with swine feces and subjected to five different intervention treatments: (i) control (untreated), (ii) water (15°C, 120 s), (iii) water followed by 2% lactic acid wash (15°C, 75 s), (iv) Combination 1 (water plus lactic acid plus hot air [510°C, 90 s]), and (v) Combination 2 (hot air plus water plus hot air). Following treatment, the pork trim was stored at 4°C for 24 h, then ground, stuffed, vacuum packaged, and stored at 4°C for 21 days. Populations of aerobic bacteria, coliforms, Escherichia coli, and lactic acid bacteria in the ground pork were monitored before treatment, after treatment (day 0), and at 2, 7, 14 and 21 days. In addition, uninoculated pork trim was treated as described above, and the color and emulsion stability of the ground product was evaluated. Ground pork prepared from trim treated with any of the treatment processes had lower initial microbial populations compared to the untreated samples. The applications of water plus lactic acid or Combination 1, which included a lactic acid wash, were more effective than water or Combination 2 at both reducing initial populations and suppressing the growth of aerobic bacteria, coliforms, and E. coli in ground pork during refrigerated storage. By day 21, populations of aerobic bacteria in ground pork prepared from control, water-treated, and Combination 2–treated trim were 8.22 to 8.32 log CFU/g, but in water plus lactic acid and Combination 1 ground pork, populations were 6.32 and 4.90 log CFU/g, respectively. Among the trim interventions examined, Combination 1 was most detrimental to the color and emulsion stability of the ground pork. The water plus lactic acid treatment provided the greatest microbial reduction and inhibition without large negative effects on quality attributes of the ground pork.

Development of a Multiple-Step Process for the Microbial Decontamination of Beef Trim†

2001 ◽  
Vol 64 (1) ◽  
pp. 63-71 ◽  
Author(s):  
DONG-HYUN KANG ◽  
MOHAMMAD KOOHMARAIE ◽  
WARREN J. DORSA ◽  
GREGORY R. SIRAGUSA
Keyword(s):  
Lactic Acid ◽  
Combination Treatment ◽  
Conveyor Belt ◽  
Microbial Reduction ◽  
Hot Water ◽  
Psychrotrophic Bacteria ◽  
Hot Air ◽  
Quality Aspects ◽  
Lean Beef ◽  
Antimicrobial Treatments

A multiple-hurdle antimicrobial process for beef trim was developed. The microbial profiles of inoculated lean beef trim tissue (BTL) and fat-covered lean beef trim (BTF) were monitored during prolonged refrigerated storage following the application of successive multiple antimicrobial treatments applied to inoculated beef trim on a processing conveyor belt set at a belt speed of 1 cm/s. Beef trim (meat size approximately 15 by 15 cm) was preinoculated with bovine feces before all treatments that included the following: control, no treatment; water wash at 65 psi for five passes; water plus lactic acid (2% [vol/vol] room temperature lactic acid wash at 30 psi for three passes); combination treatment 1 (water plus 65°C hot water at 30 psi for one pass plus hot air at 510°C for four passes plus lactic acid), combination treatment 2 (water plus hot water at 82°C for one pass plus hot air at 510°C for five passes plus lactic acid), and combination treatment 3 (water plus hot water at 82°C for three passes plus hot air at 510°C for six passes plus lactic acid). The effects of treatments on bacterial populations were monitored by enumerating mesophilic aerobic bacteria (APC), presumptive lactic acid bacteria (PLAB), psychrotrophic bacteria (PCT), coliforms, and Escherichia coli biotype 1 on product stored for up to 7 days at 4°C. In the case of BTL, the numbers of APC, PCT, and PLAB increased during storage at 5°C, whereas the numbers of coliform and E. coli decreased on average by 1.8 log CFU/cm2, then remained constant following the initial reduction. Negligible effects on color quality were observed from multihurdle treatment combination 1. In the case of the BTF, the microbial reductions by treatments were much greater than the reduction on BTL. The pH of treated BTF increased more slowly than the pH of treated BTL, resulting in further reduction of the microflora on BTF. Except for control and water treatments, all sample treatments involving lactic acid resulted in continuously decreasing microbial populations. Based on microbial reduction and quality aspects, it was concluded that successively applied combination antimicrobial treatments for meat trim could offer potential food safety benefits.

scholarly journals Hot Water Treatment in Combination with Rachis Removal and Modified Atmosphere Packaging Maintains Quality of Table Grapes

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1947-1952 ◽  
Author(s):  
Liping Kou ◽  
Yaguang Luo ◽  
Wu Ding ◽  
Xinghua Liu ◽  
William Conway
Keyword(s):  
Water Treatment ◽  
Modified Atmosphere Packaging ◽  
Storage Period ◽  
Hot Water ◽  
Microbial Populations ◽  
Modified Atmosphere ◽  
Hot Water Treatment ◽  
Table Grapes ◽  
Entire Storage Period

Alternatives to sulfur dioxide to maintain quality of table grapes, including various combinations of rachis removal, chlorinated wash, hot water treatment, and modified atmosphere packaging, were explored in this study. Grapes were prepared by cutting off the rachis 1 to 2 mm from the fruit or by keeping the clusters intact. After initial preparation, short-stem and cluster grapes were subjected to chlorinated wash and/or hot water (45 °C, 8 min) treatment and packaged in plastic trays sealed with a gas-permeable film. The treated grapes as well as the commercially packed grapes (COM) in their original packages were stored at 5 °C for up to 4 weeks. Hot water treatment resulted in significantly (P < 0.05) higher oxygen retention and lower carbon dioxide accumulation in package headspaces, maintained a firmer texture, higher overall visual quality, lower decay rate, and lower microbial populations than other treatments or COM during the entire storage period. Grapes that were cut from the rachis and treated with hot water and chlorine maintained the highest quality for 4 weeks with the least decay among all treatments. A chlorine prewash treatment significantly (P < 0.05) reduced microbial populations on cluster grapes and maintained better overall quality. Conventional COM grapes developed dark decay and lost turgidity and were of unacceptable quality at 28 days of storage.

Characterization for Staling of Chinese Rolls

2013 ◽  
Vol 798-799 ◽  
pp. 1049-1052
Author(s):  
Dong Jing Zhang ◽  
Yan Xing Zhao ◽  
Wei Yang ◽  
Yao Xu ◽  
Zheng Zhao
Keyword(s):  
Moisture Content ◽  
Storage Time ◽  
Sensory Quality ◽  
Storage Period ◽  
Enthalpy Change ◽  
Hot Water ◽  
Eating Quality ◽  
C Storage

Chinese rolls are prepared from hot water dough and wrapped with meat and vegetable when consumption. Staling of Chinese rolls results in loss in texture and eating quality. Moisture content, enthalpy change (H), texture and sensory quality of Chinese rolls which are significantly affected during staling at 4°C storage, were monitored over a storage period of four days. Moisture content was founded to decrease steadily during staling of Chinese rolls. Enthalpy change, H, as measured by DSC increased with storage time. The texture of Chinese rolls became progressively harder with storage at 4°C. A decrease in sensory quality and acceptability of the Chinese rolls was observed with storage. Most of the staling parameters show good correlation. Texture showed the best overall correlation with all other staling parameters.

scholarly journals Use of mesophilic Lactic Acid Bacteria in the manufacture of Feta cheese

2017 ◽  
Vol 56 (3) ◽  
pp. 197
Author(s):  
S. B. KARAGEORGIS (Σ.Β. ΚΑΡΑΓΕΩΡΓΗΣ) ◽  
D. K. PAPAGEORGIOU (Δ.Κ. ΠΑΠΑΓΕΩΡΓΙΟΥ) ◽  
A. I. MANTIS (Α.Ι. ΜΑΝΤΗΣ) ◽  
S. A. GEORGAKIS (Σ.Α ΓΕΩΡΓΑΚΗΣ)
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Storage Period ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Ripening Period ◽  
Psychrotrophic Bacteria ◽  
Feta Cheese ◽  
Bacterial Groups

The use of mesophilic lactic acid bacteria (LAB) in the manufacture of Feta cheese was studied. Five selected mesophilic strains, confirmed as Lactobacillus plantarum (2 strains), Lb. paracasei subsp. paracasein Lb. brevis and Lactococcus lactis subsp. lactis, isolated from ripened Feta and Teleme cheeses, were used in 7 different combinations, alone or in combination with Lb. delbrueckii subsp. bulgaricus and Streptococcus thermophilus (the control combination) for the manufacture of Feta cheese. Each combination of strains was used to prepare four different batches of Feta cheese, keeping all the other production parameters according to the traditional technology. The cheese batches were analyzed for bacteriological, chemical and sensory characteristics. The results showed that the populations of lactobacilli and lactococci increased from the beginning of the cheese manufacture reaching a population of more than 7.0 log10cfu/g. This level was maintained during the whole ripening period (60 days) and during the subsequent 60-day storage period. Only in batches prepared with the control combination F8 {Str. thermophilus I Lb. delbrueckii subsp. bulgaricus), did the population of LAB decline to 6.0 log10cfu/g at the end of the ripening period. Also, except for the yeast population which increased, in all other bacterial groups tested (coliforms, staphylococci, total contaminating bacteria, psychrotrophic bacteria, proteolytic and lipolytic bacteria) populations gradually decreased during the ripening period. Results of the chemical analysis showed a sharp increase in acidity (the cheese pH dropped to ca. 4.5 within 3-4 d) and, whereas the values of other chemical indices (moisture content, fat content and NaCl) were stabilized between the 15* and 30* day of ripening, proteolysis (nitrogen soluble in 12% TCA) and lipolysis (ADV) progressed throughout ripening. The assessment of the overall acceptance by the sensory panel was between "very good" and "excellent" for all cheeses. This suggests that the selected mesophilic starter cultures can be used alone or in combination with the traditional culture {Lb.delbrueckii subsp. bulgaricus I Str. thermophilus) in the production of Feta cheese, as the results of this work indicate that the wild (autochthonous) strains of Lb. plantarum and Lc. lactis subsp. Lactis are well adapted to the environmental conditions that prevail in Feta cheese. Batches prepared using these mesophilic starters received the highest score in the assessment of organoleptic quality of Feta cheese. Very good results were also obtained using the combination of the mesophilic starters Lb. brevis and Lc. lactis subsp. lactis or Lb. paracasei subsp. paracasei and Lc. lactis subsp. lactis alone or in combination with Lb. delbrueckii subsp. bulgaricus and Str. thermophilus.

Application of Multiple Antimicrobial Interventions for Microbial Decontamination of Commercial Beef Trim†

2001 ◽  
Vol 64 (2) ◽  
pp. 168-171 ◽  
Author(s):  
DONG-HYUN KANG ◽  
MOHAMMAD KOOHMARAIE ◽  
GREGORY R. SIRAGUSA
Keyword(s):  
Lactic Acid ◽  
Ground Beef ◽  
Microbial Reduction ◽  
Psychrotrophic Bacteria ◽  
Spray System ◽  
Hot Air ◽  
Air Gun ◽  
Quality Aspects ◽  
Set Up ◽  
Antimicrobial Interventions

Commercially produced, irregularly sized (range, 100 to 400 cm2), uninoculated beef trim was treated by a previously optimized multihurdle antimicrobial process under spray system or hot air gun with set-up speed (1 cm/s): W (water wash at 65 psi for five passes) + HW (82°C water at 30 psi for three passes) + HA (510°C air for five passes) + L (2% [vol/vol] room temperature lactic acid wash at 30 psi for three passes). After treatment, the trim was finely ground, vacuum packaged, and stored at 4°C for up to 20 days. At regular intervals (0, 5, 10, 15, and 20 days of storage at 4°C), the ground beef was analyzed to measure mesophilic aerobic bacteria (APC), coliforms, psychrotrophic bacteria (PCT), and presumptive lactic acid bacteria (PLAB) and compared with the untreated control. The numbers of APC, coliforms, PCT, and PLAB were reduced to nearly nondetectable levels immediately after treatment, with significant differences compared with the control (P &lt; 0.05), then started to increase after 5 to 10 days of storage at 4°C. After 20 days, microbial populations of treated ground beef were significantly lower than those of nontreated ground beef for the numbers of APC, coliforms, PCT, and PLAB (P &lt; 0.05), with differences of 1.2, 2.4, 1.6, and 1.6 log CFU/g, respectively. Based on microbial reduction and quality aspects, the multihurdle antimicrobial process was identified as an effective intervention to reduce coliforms on beef trim.

Variations in External and Internal Microbial Populations in Shell Eggs during Extended Storage

2004 ◽  
Vol 67 (12) ◽  
pp. 2657-2660 ◽  
Author(s):  
D. R. JONES ◽  
M. T. MUSGROVE ◽  
J. K. NORTHCUTT
Keyword(s):  
Storage Period ◽  
Microbial Populations ◽  
Aerobic Bacteria ◽  
Microbial Quality ◽  
Shell Eggs ◽  
External Shell ◽  
Processing Line ◽  
Yeasts And Molds

The current project was conducted to determine the microbial quality of commercially processed shell eggs during extended storage. Unwashed eggs were collected at the accumulator before entering the processing line. Washed eggs were retrieved after placement in flats. All eggs were stored on pulp flats at 4°C for 10 weeks. Twelve eggs from each treatment were rinsed on the day of collection and during each week of storage. After rinsing, eggs were sanitized in ethanol, and contents were aseptically collected. Total aerobes, yeasts and molds, Enterobacteriaceae, and pseudomonads were enumerated from shell rinses and pooled egg contents. During storage, no differences were found between unwashed and washed eggs for Enterobacteriaceae and pseudomonads in either shell rinses or contents. No differences were found between treatments for population levels of total aerobes or yeasts and molds in the egg contents throughout the storage period. Significant differences between treatments were found at each week of storage for external shell contamination by total aerobes. The highest unwashed egg contamination occurred at week 8 of storage and the lowest was at weeks 0 and 1 of storage. The highest shell contamination with aerobic bacteria on the washed eggs was found at week 0 of storage and the lowest was at week 7. Yeast and mold contamination determined by shell rinses was also significantly different between treatments at each week of storage. Commercially washed eggs were significantly less contaminated than were unwashed eggs for the populations monitored.

scholarly journals (471) Application of Mustard and Hop Extract Agents Mixed with Sucrose Fatty Acid Esters as a Food Preservative for Fresh-cut Vegetables

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1092A-1092
Author(s):  
Daisuke Hamanaka ◽  
Takashi Watakabe ◽  
Kazuyuki Kitano ◽  
Hidemi Izumi
Keyword(s):  
Lactic Acid ◽  
Fatty Acid ◽  
Lactic Acid Bacteria ◽  
Ethylene Production ◽  
Storage Period ◽  
Fatty Acid Esters ◽  
Aerobic Bacteria ◽  
Ma Packaging ◽  
Sucrose Fatty Acid Esters ◽  
Acid Esters

Cabbage shreds and cucumber slices were dipped in water or solutions of mustard extract agent (WASAOURO®, Mitsubishi-Kagaku Foods Corp.) and hop extract agent (HOPREX®, Mitsubishi-Kagaku Foods Corp.) with or without sucrose fatty acid esters (SE; Mitsubishi-Kagaku Foods Corp.) and stored in MA packaging at 10 °C. With cabbage shreds, treatment with mustard extract agent reduced the depletion of O2 and accumulation of CO2 and ethylene in MA packaging and the reduction was greater when mixed with SE. Treatment with the combination of mustard and hop extract agents with SE also had suppressing effects on respiration and ethylene production of cabbage shreds throughout the MA storage period. Counts of mesophilic aerobic bacteria and coliforms were 0.3 to 0.7 logs lower in cabbage shreds treated with mustard extract agent with or without SE than with the water-dipped control for the first 2 days of storage at 10 °C. However, mustard extract agent stimulated the growth of lactic acid bacteria. When hop extract agent was combined with mustard extract agent with SE, counts of mesophilic aerobic bacteria, coliforms, and lactic acid bacteria on treated cabbage shreds were reduced by 0.8, 1.6, and 2.6 logs, respectively, relative to control samples after 5 days of storage. Cucumber slices treated with mustard extract agent with or without SE accelerated respiration and ethylene production and did not retard any bacterial growth during MA storage at 10 °C.

scholarly journals Formation of Biogenic Amines in Chicken Meat Stored under Modified Atmosphere

2010 ◽  
Vol 79 (9) ◽  
pp. S107-S116 ◽  
Author(s):  
Leo Gallas ◽  
Eva Standarová ◽  
Iva Steinhauserová ◽  
Ladislav Steinhauser ◽  
Lenka Vorlová
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biogenic Amines ◽  
Storage Period ◽  
Coliform Bacteria ◽  
Modified Atmosphere ◽  
Psychrotrophic Bacteria ◽  
Modified Atmospheres ◽  
Brochothrix Thermosphacta ◽  
Microbiological Indicators

The aim of the study was to investigate the effects of two modified atmospheres with a different combination of gases on selected groups of microorganisms and on concentrations of biogenic amines (BAs) in samples of poultry breast muscle. The samples were packaged under modified atmosphere A (75% O2 a 25% CO2) or B (75% N2 and 25% CO2) and stored at temperatures from +2 to +4 °C for 14 days. During the storage period, O2 concentrations in modified atmosphere A (MA A) decreased from the initial 74.8 ± 0.3% to 55.9 ± 6.6% at the end of the storage period. In all samples, counts of psychrotrophic bacteria counts, Brochothrix thermosphacta, lactic acid bacteria and coliform microorganism were determined. The tests were made on the packaging day, and then after three, nine and fourteen days of storage. At the end of the storage period, higher numbers of psychrotrophic bacteria (6.5 ± 0.7 log10 cfu g-1), Brochothrix thermosphacta (4.8 ± 0.3 log10 cfu g-1) and lactic acid bacteria (1.7 ± 0.4 log10 cfu g-1) were found on samples packaged under MA A. Samples packaged under modified atmosphere B on the other hand contained higher numbers of coliform bacteria (4.1 ± 0.6 log10 cfu g-1) at the end of the storage period. In addition to microbiological indicators, concentrations of biogenic amines (putrescine, cadaverine, histamine, tyramine, spermine, spermidine and β-phenylethylamine) were also determined. In fresh samples and after three days of storage, only spermine and spermidine were found. After 9 and 14 days, also other BAs were detected. The biogenic amine totals at the end of the storage period was 60.0 ± 13.2 mg kg-1 in samples packaged under MA A and 129.0 ± 41.3 mg kg-1 in samples packaged under MA B. The most abundantly represented biogenic amines in samples packaged under MA A were putrescine and spermine (49.7 and 24.8%, respectively, at the end of the storage period), and putrescine and cadaverine in samples packaged under MA B (47.0 and 32.9 %, respectively, at the end of the storage period).

scholarly journals Prestorage Heat Treatment to Inhibit Chilling Injury and Delay Ripening in Tomato Fruits

1993 ◽  
Author(s):  
Susan Lurie ◽  
David R. Dilley ◽  
Joshua D. Klein ◽  
Ian D. Wilson
Keyword(s):  
Heat Treatment ◽  
Chilling Injury ◽  
Storage Period ◽  
Membrane Lipid ◽  
Hot Water ◽  
Low Temperature Storage ◽  
Hot Air ◽  
Subsequent Storage ◽  
Shock Proteins ◽  
Temperature Storage

The research had two specific goals; (1) to develop and optimize a postharvest heat treatment and characterize the response of tomato to the heat and subsequent cold storage, and (2) to investigate the involvement of heat shock proteins (HSP) in resistance to chilling injury. For the first goal we have investigated many time-temperature treatments using dry heat and found that 48 h at 38oC is optimum for Israeli cultivars, while 48 h at 42oC worked better for American cultivars in preventing chilling injury. We have also compared hot water to hot air and found hot water to be effective, but less so than hot air. Membrane lipid composition in relation to chilling injury was investigated after hot water and hot air treatments. Investigation of fruit ripening found that mRNAs of ripening-related genes were inhibited by high temperature, but recovered during the subsequent storage period and allowed normal ripening to proceed. Sensory studies showed no difference in the taste of heated or nonheated fruit. Following the production of HSP in heated and stored fruit allowed us to determine that during low temperature storage the HSP remained present in the fruit tissue, and their presence was correlated with resistance to chilling injury. HSP clones have been isolated by both differential screening of a cDNA library of heated and chilled tomatoes (Israel) and by mRNA differential display (United States). These clones are being characterized.

Sign in / Sign up

Export Citation Format

Share Document