Synergistic Inhibitory Effect of Citral with Selected Phenolics against Zygosaccharomyces bailii

2005 ◽  
Vol 68 (3) ◽  
pp. 602-606 ◽  
Author(s):  
KARLA RIVERA-CARRILES ◽  
ALVARO ARGAIZ ◽  
ENRIQUE PALOU ◽  
AURELIO LÓPEZ-MALO
Keyword(s):  
Phenolic Compounds ◽  
Water Activity ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Inhibitory Effect ◽  
The Other ◽  
Zygosaccharomyces Bailii ◽  
Ph Conditions ◽  
Synergistic Inhibitory Effect ◽  
Synergistic Combinations

Antifungal susceptibilities of Zygosaccharomyces bailii to individual and binary mixtures of citral with selected phenolics were evaluated to identify synergistic combinations. Individual effects of citral, vanillin, thymol, carvacrol, and eugenol concentrations and combined effects of citral with the other phenolic compounds on the growth of Z. bailii were evaluated in potato dextrose agar, adjusted with sucrose to a water activity of 0.99 or 0.95, and hydrochloric acid to pH 4.5 or 3.5. MICs for individual and binary antimicrobial mixtures were identified and then transformed to fractional inhibitory concentrations. Inhibitory concentrations of citral and vanillin were higher than 650 ppm, whereas for thymol, eugenol, and carvacrol, concentrations were lower than 250 ppm for several of the studied water activity–pH conditions. Combining citral with the other phenolic compounds, fractional inhibitory concentration (FIC) and FICIndex varied from 0.216 to 0.582. FICIndex demonstrated synergistic effects on Z. bailii inhibition when citral was used in combination with vanillin, thymol, carvacrol, or eugenol. Therefore, the relative amount of antimicrobials could be greatly reduced.

Individual and Combined Effects of Vanillin and Potassium Sorbate on Penicillium digitatum, Penicillium glabrum, and Penicillium italicum Growth

1999 ◽  
Vol 62 (5) ◽  
pp. 541-542 ◽  
Author(s):  
B. MATAMOROS-LEÓN ◽  
A. ARGAIZ ◽  
A. LÓPEZ-MALO
Keyword(s):  
Water Activity ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Potato Dextrose Agar ◽  
Penicillium Digitatum ◽  
Potassium Sorbate ◽  
Combined Effects ◽  
Fractional Inhibitory Concentration ◽  
Penicillium Italicum ◽  
The Individual

The individual and combined effects of potassium sorbate and vanillin concentrations on the growth of Penicillium digitatum, P. glabrum, and P. italicum in potato dextrose agar adjusted to water activity 0.98 and pH 3.5 were evaluated. Inhibitory concentrations of potassium sorbate varied from 150 ppm for P. digitatum to 700 ppm for P. glabrum, and for vanillin from 1,100 ppm for P. digitatum and P. italicum and 1,300 ppm for P. glabrum. Fractional inhibitory concentration (FIC) isobolograms show curves deviated to the left of the additive line. Calculated FIC index varied from 0.60 to 0.84. FIC index as well as FIC isobolograms show synergistic effects on mold inhibition when vanillin and potassium sorbate are applied in combination.

scholarly journals Synergistic anti-biofilm effects of Brassicaceae plant extracts in combination with proteinase K against Escherichia coli O157:H7

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wen Si Hu ◽  
Da Min Nam ◽  
Joo-Sung Kim ◽  
Ok Kyung Koo
Keyword(s):  
Stainless Steel ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Combined Treatment ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Proteinase K ◽  
E Coli ◽  
Log Reduction ◽  
Food Contact Surfaces

AbstractBacteria can form biofilms, complex microbial communities protected from environmental stress, on food contact surfaces. Brassicaceae plant has been shown to contain bioactive compounds with antimicrobial activities. The objective of this study was to evaluate the synergistic effects of Brassicaceae species and proteinase K against E. coli O157:H7 biofilm. We determined the minimum biofilm inhibitory concentration, the fractional inhibitory concentration indexes, and the synergistic inhibitory effect of Raphanus sativus var. longipinnatus, R. sativus, and Brassica oleracea var. acephala extracts with proteinase K on E. coli O157:H7. The biofilm showed a 49% reduction with 2 mg/mL R. sativus. The combination of proteinase K 25 µg/mL significantly increased the effect of 2 mg/mL R. sativus var. longipinnatus and the combined treatment yielded up to 2.68 log reduction on stainless steel coupons. The results showed that the combination of R. sativus var. longipinnatus extract and proteinase K could serve as an anti-biofilm agent with synergistic effects for inhibiting E. coli O157:H7 biofilm on stainless steel surfaces.

Anti-adenovirus activity, antioxidant potential, and phenolic content of black tea (Camellia sinensis Kuntze) extract

2016 ◽  
Vol 13 (4) ◽  
Author(s):  
Ali Karimi ◽  
Mohammad-Taghi Moradi ◽  
Somayeh Alidadi ◽  
Leila Hashemi
Keyword(s):  
Phenolic Compounds ◽  
Antiviral Activity ◽  
Inhibitory Concentration ◽  
Phenolic Content ◽  
Black Tea ◽  
Inhibitory Effect ◽  
Hep2 Cell ◽  
Black Tea Extract ◽  
Tea Extract ◽  
Specific Antiviral Therapy

AbstractBackgroundAdenovirus (ADV) causes a number of diseases in human, and to date, no specific antiviral therapy is approved against this virus. Thus, searching for effective anti-ADV agents seems to be an urgent requirement. Many studies have shown that components derived from medicinal plants have antiviral activity. Therefore, the present study was aimed to evaluateMethodsIn this study, the hydroalchoholic extract of black tea was prepared and its anti-ADV activity was evaluated on HEp2 cell line using MTT [3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] assay. The 50 % inhibitory concentration (ICResultsThe CCConclusionsHaving SI value of 25.06 with inhibitory effect on ADV replication, particularly during the post-adsorption period, black tea extract could be considered as a potential anti-ADV agent. The antiviral activity of this extract could be attributed to its phenolic compounds.

scholarly journals Inhibitory Effect of Natural Phenolic Compounds onAspergillus parasiticusGrowth

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Romina P. Pizzolitto ◽  
Carla L. Barberis ◽  
José S. Dambolena ◽  
Jimena M. Herrera ◽  
María P. Zunino ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Water Partition Coefficient ◽  
Phenolic Components ◽  
Natural Phenolic Compounds ◽  
The Individual ◽  
New Compounds ◽  
The Impact

Considering the impact ofAspergillusspecies on crops, it appears to be highly desirable to apply strategies to prevent their growth, as well as to eliminate or reduce their presence in food products. For this reason, the aims of this investigation were to evaluate the effects of ten natural phenolic compounds on theAspergillus parasiticusgrowth and to determine which physicochemical properties are involved in the antifungal activity. According to the results of minimum inhibitory concentration (MIC) values of the individual compounds, isoeugenol, carvacrol, and thymol were the most active phenolic components (1.26 mM, 1.47 mM, and 1.50 mM, resp.), followed by eugenol (2.23 mM). On the other hand, creosol, p-cresol, o-cresol, m-cresol, vanillin, and phenol had no effects on fungal development. Logarithm of the octanol/water partition coefficient (log P), refractivity index (RI), and molar volume (MV) were demonstrated to be the descriptors that best explained the antifungal activity correlated to lipophilicity, reactivity of the components, and steric aspect. These findings make an important contribution to the search for new compounds with antifungal activity.

scholarly journals Antimigratory Activity of Brazilin-Containing Fraction from Caesalpinia sappan L. on MDAMB-231 Cells

2020 ◽  
Vol 27 (4) ◽  
pp. 266
Author(s):  
Sri Handayani ◽  
Ratna Asmah Susidarti ◽  
Puspa Dewi Narrij Lotulung ◽  
Akhmad Darmawan ◽  
Edy Meiyanto ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Combination Index ◽  
Synergistic Effects ◽  
Biological Activities ◽  
Gelatin Zymography ◽  
Inhibitory Effect ◽  
Cytotoxic Effects ◽  
Caesalpinia Sappan ◽  
Liquid Liquid Extraction ◽  
Synergistic Inhibitory Effect

Caesalpinia sappan is studied for several biological activities. The aim of this research is to determine the cytotoxic and antimigratory activities of Caesalpinia sappan active fraction in combination with cisplatin on human TNBC cells (MDA-MB-231). Caesalpinia sappan heartwood was extracted with methanol. Then, several fractions of the methanol extract were obtained by using a liquid-liquid extraction method followed by column chromatography. The cytotoxicity was determined using MTT assay. Synergistic effects were analyzed by calculating the combination index (CI). Migration was examined using wound-healing assay. Levels of MMP2 activity were determined with gelatin zymography assay. The results showed that most of the fractions included in this study exhibited cytotoxic effects against MDA-MB-231 cells, and C fraction demonstrated the highest cytotoxic activity of all fractions. The combination of C-cisplatin revealed a synergistic inhibitory effect on MDA-MB-231 cell growth (CI<1). Furthermore, C fraction, alone and in combination with cisplatin, inhibited migration of MDA-MB-231 and suppressed MMP2 activity. The C fraction isolated from Caesalpinia sappan increased the cytotoxic and antimigratory activities of cisplatin on MDA-MB-231 cells. Based on these findings, the potential of Caesalpinia sappan to act as a supportive agent in metastatic TNBC treatment with cisplatin warrants further exploration.

scholarly journals Human recombinant lactoferrin acts synergistically with antimicrobials commonly used in neonatal practice against coagulase-negative staphylococci and Candida albicans causing neonatal sepsis

2008 ◽  
Vol 57 (9) ◽  
pp. 1113-1121 ◽  
Author(s):  
Mohan Pammi Venkatesh ◽  
Liang Rong
Keyword(s):  
Neonatal Sepsis ◽  
Synergistic Effects ◽  
Inhibitory Effect ◽  
Drug Dose ◽  
Coagulase Negative Staphylococci ◽  
Mortality And Morbidity ◽  
Dose Effects ◽  
Synergistic Combinations ◽  
Antimicrobial Combinations ◽  
Significant Mortality

Neonatal sepsis causes significant mortality and morbidity. Coagulase-negative staphylococci (CoNS) and Candida frequently cause neonatal sepsis at >72 h of age. Lactoferrin, which is present in human milk, is a component of innate immunity and has broad-spectrum antimicrobial activity. The synergistic effects of lactoferrin with antibiotics against neonatal isolates have not been systematically evaluated. Here, eight clinical strains (seven neonatal) of CoNS and three strains (two neonatal) of Candida albicans were studied. MIC50 and MIC90 values of human recombinant lactoferrin (talactoferrin; TLF), vancomycin (VAN) and nafcillin (NAF) against CoNS, and of TLF, amphotericin B (AMB) and fluconazole (FLC) against C. albicans, were evaluated according to established guidelines. Antimicrobial combinations of TLF with NAF or VAN against CoNS, and TLF with AMB or FLC against C. albicans, were evaluated by a chequerboard method with serial twofold dilutions. Synergy was evaluated by the median effects principle, and combination indices and dose reduction indices were reported at 50, 75 and 90 % inhibitory effect at several drug-dose ratios. It was found that TLF acted synergistically with NAF and VAN against CoNS, and with AMB and FLC against C. albicans, at multiple dose effects and drug-dose ratios with few exceptions. In synergistic combinations, drug reduction indices indicated a significant reduction in doses of antibiotics, which may be clinically relevant. Thus TLF acts synergistically with anti-staphylococcal and anti-Candida agents commonly used in neonatal practice and is a promising agent that needs to be evaluated in clinical studies.

scholarly journals Addition of microencapsulated soybean molasses to pasta formulations

2021 ◽  
Vol 51 (9) ◽  
Author(s):  
Vanessa Sabrina Fagundes Batista ◽  
Graciele Lorenzoni Nunes ◽  
Andressa Fraton Moreira da Silva ◽  
Gabriela Ilha Viegas ◽  
Bruna Nichelle Lucas ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Shelf Life ◽  
Sensory Evaluation ◽  
Water Activity ◽  
The Other ◽  
Sensory Acceptability ◽  
Microbiological Profile ◽  
Color Measurements ◽  
Microbiological Characteristics

ABSTRACT: This study investigated the production of pasta with the addition of microencapsulated soybean molasses and its effect on the physicochemical, sensory, and microbiological characteristics and shelf life of the product. Three formulations were prepared, as follows: F1, control pasta; F2, pasta made with free extract; and F3, pasta made with soybean molasses microparticles, which were characterized in terms of their pH, water activity, color measurements, sensory evaluation, microbiological characterization, and shelf life via determination of the phenolic compounds remaining during a 28 day storage. The cooked pasta containing the microparticles retained 25% of the original phenolics up to 14th day of storage, meanwhile, the raw pasta presented 15% of the original phenolics up to the 21st day of storage. Good sensory acceptability was observed for all pasta containing the microparticles, with no differences from the control pasta; however, pasta made with free extract was less accepted when compared to the other types. The addition of microparticles did not change the microbiological profile, pH, water activity, or color of the pasta.

Synergistic Antitumor Effect of 5-Fluorouracil Combined with Constituents from Pleurospermum lindleyanum in Hepatocellular carcinoma SMMC-7721 Cells

2020 ◽  
Vol 20 ◽  
Author(s):  
Xiao-Feng Zhu ◽  
Xiao-Jin Li ◽  
Zhong-Lian Cao ◽  
Xiu-Jie Liu ◽  
Ping Yang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Treatment Effectiveness ◽  
Synergistic Effects ◽  
Folk Medicine ◽  
Inhibitory Effect ◽  
Caspase 9 ◽  
Whole Plant ◽  
Anti Cancer ◽  
Induced Apoptosis

Background: A Chinese folk medicine plant Pleurospermum lindleyanum possesses pharmacological activities of heat-clearing, detoxifying and preventing from hepatopathy, coronary heart disease, hypertension, and high altitude sickness. We isolated and characterized its constituents to investigate its synergistic effects against human hepatoma SMMC-7721 cells. Objective: The aim of this study was to explore the synergistic anti-cancer activities of isolates from P. lindleyanum with 5-FU on hepatoma SMMC-7721 cells in vitro and their primary mechanisms. Methods: Sequential chromatographic techniques were conducted for the isolation studies. The isolates structures were established by spectroscopic analysis as well as X-ray crystallographic diffraction. Growth inhibition was detected by MTT assay. The isobologram method was used to assess the effect of drug combinations. Flow cytometry and western blot were used to examine apoptosis and protein expression. Results: A new coumarin (16), along with sixteen known compounds, were isolated from the whole plant of P. lindleyanum and their structures were elucidated by spectroscopic methods. Four coumarins (2, 3, 5, and 16), two flavonoids (8 and 9) and three phytosterols and triterpenes (12-14) were found to synergistically enhance the inhibitory effect of 5-FU against SMMC-7721 cells. Among them, compounds 3 and 16 exhibited the best synergistic effects with IC50 of 5-FU reduced by 16-fold and 22-fold possessing the minimum Combination Index (CI) 0.34 and 0.27. The mechanism of action of combinations might be through synergistic arresting for the cell cycle at G1 phases and the induction of apoptosis. Moreover, western blotting and molecular docking revealed that compounds 3 or 5 might promote 5-FU-induced apoptosis by regulating the expression of Caspase 9 and PARP. Conclusion: Constituents from P. lindleyanum may improve the treatment effectiveness of 5-FU against hepatocellular carcinoma cells.

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