Detection of Mycobacterium tuberculosis Complex by PCR in Fresh Cheese from Local Markets in Hidalgo, Mexico

2014 ◽  
Vol 77 (5) ◽  
pp. 849-852 ◽  
Author(s):  
ANA LAURA PEREIRA-SUÁREZ ◽  
YESSICA ESTRADA-CHÁVEZ ◽  
ARMIDA ZÚÑIGA-ESTRADA ◽  
GONZÁLO LÓPEZ-RINCÓN ◽  
DAVID ULISES MIRANDA HERNÁNDEZ ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Mitochondrial Gene ◽  
Pcr Amplification ◽  
Raw Milk ◽  
Cyt B ◽  
Tuberculosis Complex ◽  
Bacterial Dna ◽  
Fresh Cheese ◽  
Exogenous Control

The objective of this study was to identify the presence of Mycobacterium tuberculosis complex bacterial DNA in samples extracted from fresh cheeses; 95 samples of fresh cheese were obtained from municipal markets in the state of Hidalgo, in central Mexico, and were analyzed in triplicate. The exogenous control for the amplification was the mitochondrial gene for cytochrome b (cyt-b). M. tuberculosis complex DNA was detected by nested-PCR amplification of a fragment of the mpb70 gene in six samples, four of which were obtained from regions with enzootic bovine tuberculosis. These results suggest that cheeses prepared with raw milk contaminated with M. bovis are being sold and consumed by humans, which may cause tuberculosis.

MILK CONTAMINATION BY Mycobacterium tuberculosis complex, IMPLICATIONS FOR PUBLIC HEALTH IN AMAZONAS, BRAZIL

2021 ◽  
Author(s):  
Paulo A.M. Carneiro ◽  
Taynara N. Pasquatti ◽  
Daiane A.R. Lima ◽  
Rudiele A. Rodrigues ◽  
Haruo Takatani ◽  
...  
Keyword(s):  
Public Health ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Genetic Material ◽  
Raw Milk ◽  
Buffalo Milk ◽  
The State ◽  
Metagenomic Sequencing ◽  
Tuberculosis Complex ◽  
Shotgun Metagenomic Sequencing

In Brazil, contamination of raw milk with Mycobacterium tuberculosis complex (MTC) has been reported in several states. In Amazonas, the rate of consumption of raw milk and its derivatives is the highest in the country. The state has the highest prevalence of tuberculosis in both humans and livestock. Therefore, we assessed the contamination of cattle and buffalo milk with MTC in the state of Amazonas, focusing on Mycobacterium bovis (M. bovis) , the species most commonly originating in cattle and buffalo. In 2019, 250 samples of raw milk (91 cattle; 159 buffalo) were collected from three milk plants, before the processing, in the state of Amazonas. The samples were placed into 21 pools then analyzed using Shotgun Metagenomic Sequencing and taxonomic classification by Kraken-2 and MegaBLAST. To confirm the identity of Mycobacterial species found, BLASTN was used to identify specific genomic positions at the TbD1 and RD1 regions and flanking RD4 region. MTC species genetic material were identified in all pools of raw milk. Genetic material consistent with M. bovis were identified in seven pools of raw milk (1 cattle and 6 buffalo). Milk from buffalo presented significantly higher MTC reads than milk from cattle. The common practice of consumption of raw milk and its derivatives in Amazonas presents an imminent risk to public health. Urgent measures to prevent transmission of foodborne tuberculosis are needed in the Amazon region. Greater efforts and resources should also be directed towards elimination of bovine tuberculosis disease in cattle and buffalo herds in the state of Amazonas and all of Brazil.

scholarly journals Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1294
Author(s):  
Cristina Blanco Blanco Vázquez ◽  
Thiago Doria Barral ◽  
Beatriz Romero ◽  
Manuel Queipo ◽  
Isabel Merediz ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Road Traffic ◽  
Mycobacterium Tuberculosis Complex ◽  
Temporal Distribution ◽  
Meles Meles ◽  
Interspecies Transmission ◽  
Tuberculosis Complex ◽  
Serum Samples ◽  
Study Objective ◽  
Infection Pressure

The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test results were extracted for cattle that were tested and culled as part of the Spanish National Program for the Eradication of Bovine TB. A total of 27/639 badgers (4.23%) were positive for MTC based on bacterial isolation, while 160/673 badgers (23.77%) were found to be positive with the P22 ELISA. The rate of seropositivity was higher among adult badgers than subadults. Badger TB status was spatially and temporally associated with cattle TB status. Our results cannot determine the direction of possible interspecies transmission, but they are consistent with the idea that the two hosts may exert infection pressure on each other. This study highlights the importance of the wildlife monitoring of infection and disease during epidemiological interventions in order to optimize outcomes.

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