scholarly journals Detection of smut mycelia in apical meristems of sugarcane buds

1969 ◽  
Vol 75 (3) ◽  
pp. 281-286
Author(s):  
Rodrigo Echávez-Badel

Two tests were conducted to detect the fungal mycelia of Ustilago scitaminea Syd. in apical meristems of sugarcane (Saccharum officinarum L.) buds. In the first test six varieties were selected from infected sugarcane fields, and in the second test three varieties obtained from a nursery field were artificially inoculated with the fungus. Sugarcane plants artificially inoculated with U. scitaminea were used as checks in the first test. Growing points were removed from the plant cane and stained for 4 to 18 h by using Sinha's technique. Microscopic observations indicated the absence and presence of smut mycelia in the apical meristem buds of healthy and infected sugarcane varieties. The staining method of growing point nodal buds can be useful for indicating fungicide efficacy in controlling sugarcane smut, and for sugarcane seed certification programs.

2020 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Dentin Queentiara Moelyaandani ◽  
S Setiyono

Sugarcane (Sacharum officinarum L.) is a sugar-producing plant and is a food commodity that is needed by the people of Indonesia. National sugar demands are increasing every year. The low productivity of sugarcane is due to the competition of weeds which can reduce sugarcane production. To achieve high productivity, it is necessary to pay attention to the varieties used because they require certain conditions in their management. The purpose of this study was to determine the effect of varieties and types of weeds on the initial growth of sugarcane. The study was conducted from September 2018 to December 2018. The study used factorial Randomized Block Design (RBD) with two factors and four replications. The first factor consists of 4 sugarcane varieties, namely: Bululawang (V1), Red HW (V2), VMC (V3) and PS 862 (V4). The second factor type of weed consists of 2 types, namely Cyperus rotundus (N1) and Amaranthus spinosus L. (N2). The results showed that (1) There was interaction between the varieties (V) and the weed types (N) in stem diameter parameters and number of tillers, the treatment V4N1 gives the best response to stem diameter 1,31 cm and 4,25 number of tillers; (2) The treatment of varieties (V) gives affects to stem diameter and number of leaves, the treatment V4 gives the best response 5,38 to number of leaves; (3) Types of thorn spinach weeds can reduce the initial growth of sugarcane. Keywords: Varieties; weed; sugarcane


2021 ◽  
Vol 10 (1) ◽  
pp. 40-52
Author(s):  
Ade Astri Muliasari ◽  
Ranu Trilaksono

ABSTRACTPest and disease monitoring aim to determine the type, sign/symptoms, and intensity of attacks. The rat (Bandicota indica), stem borer (chilo sacchariphagus Bojer), and shoot borer (Scirpophaga excerptalis Walker) place major constraints on a sugarcane plantation in PT PG Rajawali Jatitujuh Majalengka districts. The intensity of attacks (IS) of these pests reached 8.6%, 1.26%, and 0.86%, respectively. The intensity of rats reached as high as more than 5%. Shoot borer and stem borer attack are low under 5%. The smut disease caused by the fungus Ustilago scitaminea Sydow with an attacking intensity of 1.53% and belongs to the low category. Integrated pest and disease control include technical, physical / mechanical, biological, and chemical culture. ABSTRAKMonitoring hama dan penyakit bertujuan untuk mengetahui jenis, gejala, insidensi serangan dan cara pengendaliannya. Hama utama tebu di PT PG Rajawali II Jatitujuh Majalengka yaitu hama tikus wirok (Bandicota indica Bechstein), penggerek batang tebu/stem borer (Chilo sacchariphagus Bojer), dan penggerek pucuk tebu/shoot borer (Scirpophaga excerptalis Walker). Insidensi serangan (IS) masing-masing mencapai 8,6%, 1,26% dan 0,86%. Insidensi serangan hama tikus tergolong tinggi dengan standar ambang batas ekonomi yang ditentukan yaitu 0%. Insidensi serangan hama penggerek tebu termasuk kategori ringan yaitu di bawah ambang ekonomi 5%. Penyakit utama yaitu luka api/cambuk jelaga yang disebabkan jamur Ustilago scitaminea Sydow dengan insidensi serangan 1.53% dan termasuk kategori ringan. Pengendalian hama dan penyakit dilakukan secara terpadu meliputi kultur teknis, fisik/mekanis, biologi dan kimia.


2020 ◽  
Vol 21 (12) ◽  
pp. 4295 ◽  
Author(s):  
Rafael Cruz ◽  
Gladys F. A. Melo-de-Pinna ◽  
Alejandra Vasco ◽  
Jefferson Prado ◽  
Barbara A. Ambrose

Unlike seed plants, ferns leaves are considered to be structures with delayed determinacy, with a leaf apical meristem similar to the shoot apical meristems. To better understand the meristematic organization during leaf development and determinacy control, we analyzed the cell divisions and expression of Class I KNOX genes in Mickelia scandens, a fern that produces larger leaves with more pinnae in its climbing form than in its terrestrial form. We performed anatomical, in situ hybridization, and qRT-PCR experiments with histone H4 (cell division marker) and Class I KNOX genes. We found that Class I KNOX genes are expressed in shoot apical meristems, leaf apical meristems, and pinnae primordia. During early development, cell divisions occur in the most distal regions of the analyzed structures, including pinnae, and are not restricted to apical cells. Fern leaves and pinnae bear apical meristems that may partially act as indeterminate shoots, supporting the hypothesis of homology between shoots and leaves. Class I KNOX expression is correlated with indeterminacy in the apex and leaf of ferns, suggesting a conserved function for these genes in euphyllophytes with compound leaves.


2014 ◽  
Vol 5 (2) ◽  
pp. 85 ◽  
Author(s):  
Ejiroghene Felix Lawyer ◽  
Z. O. Jamaleddine ◽  
P. T. Lyam ◽  
I. T. Borokini ◽  
A. A. Adedeji ◽  
...  

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.


2020 ◽  
Vol 42 ◽  
Author(s):  
Mirella Figueiró de Almeida ◽  
Sarah da Silva Costa ◽  
Iara Eleutéria Dias ◽  
Carolina da Silva Siqueira ◽  
José da Cruz Machado

Abstract: Cotton Ramulosis (Gossypium hirsutum) is an important disease affecting cotton plantations in Brazil, and its causal agent, Colletotrichum gossypiivar.cephalosporioides(Cgc), according to the Brazilian phytosanitary authority, was considered a regulated non quarantine pest. It makes this microorganism subject to standardization in seed certification programs. The current seed health testing for detecting that pathogen in seed samples does not provide reliable results for routine analysis. On this paper, attempts were made to design specific primers for detection of Cgc associated with cotton seed. Two primer sets were selected based on the analysis of a multiple alignment of gene’s sequence encoding the glyceraldehyde 3-phosphate dehydrogenase from Cgc, C. gossypii and reference strains of the C. gloeosporioides species complex. The conserved sites unique to Cgc strains were used to design specific fragment of 140 bp. The primer specificity was confirmed by using other fungi. The primers produced a detectable band of target DNA of Cgc in all inoculum potentials of the pathogen artificially inoculated by the water restriction technique. The developed primer pair represents, therefore, a reliable and rapid mean to diagnose the Ramulosis agent in cotton seed.


Irriga ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 449-464
Author(s):  
Eduardo Magno Pereira da Silva ◽  
Aderson Soares de Andrade Júnior ◽  
Edson Alves Bastos ◽  
Valdenir Queiroz Ribeiro

PERFORMANCE AGROINDUSTRIAL DE CANA-DE-AÇÚCAR EM DIFERENTES REGIMES HÍDRICOS     EDUARDO MAGNO PEREIRA DA SILVA1; ADERSON SOARES DE ANDRADE JÚNIOR2; EDSON ALVES BASTOS2 E VALDENIR QUEIROZ RIBEIRO2   1Instituto Federal de Educação, Ciência e Tecnologia do Piauí - Campus Uruçuí Rodovia PI 247, S/N - Portal dos Cerrados, CEP: 64860-000, Uruçuí, Piauí, Brasil, e-mail: [email protected] 2Embrapa Meio‑Norte, Avenida Duque de Caxias, n° 5.650, Bairro Buenos Aires, CEP64006‑220, Teresina, Piauí, Brasil, e-mail: [email protected], [email protected], [email protected]     1 RESUMO   O objetivo desta pesquisa foi avaliar a performance agroindustrial de cana-de-açúcar (planta) em resposta a diferentes regimes hídricos aplicados por gotejamento subsuperficial. O experimento foi realizado em um argissolo vermelho amarelo distrófico, na Embrapa Meio-Norte, em Teresina, Piauí, durante o período de outubro de 2015 a setembro de 2016. Modelou-se o experimento em delineamento experimental de blocos casualizados em parcelas subdivididas, com quatro repetições. As parcelas foram constituídas por quatro regimes hídricos (irrigação mais precipitação pluviométrica) baseados em frações da evapotranspiração de cultura (50% ETc = 1.374,3; 80% ETc = 1.534,2; 110% ETc = 1.712,0 e 150% ETc = 1.905,8 mm) e as subparcelas por dez variedades de cana-de-açúcar (RBUFRPE01, RBUFRPE02, RBUFRPE03, RBUFV01, RB036066, RB987935, RB935744, RB943538, RB92579 e RB867515). As características tecnológicas de qualidade do caldo da cana e rendimentos de açúcar e álcool foram alterados pelos regimes hídricos e variedades. Os maiores teores de Brix (22,5%), Fibras (13,3%), Polcaldo (17,5%) e Polcana (13,9%) foram obtidos com as variedades RB867515, RB935744, RB943538 e RB935744, respectivamente. Os rendimentos de açúcar (32,2 Mg ha-1) e álcool (23 m3 ha-1) foram alcançados com a variedade RB935744 e reposição hídrica próxima a 110% da ETc.   Palavras-chave: Saccharum officinarum, eficiência da água, manejo da irrigação.     SILVA, E. M. P.; ANDRADE JÚNIOR, A. S.; BASTOS, E. A.; RIBEIRO, V. Q. AGRO-INDUSTRIAL PERFORMANCE OF SUGARCANE IN DIFFERENT WATER REGIMES   2 ABSTRACT   The objective of this research was to evaluate the agro-industrial performance of sugarcane varieties (cane plant) in response to different water regimes applied by subsurface drip irrigation. The experiment was carried out in a dystrophic yellow red acrisol at Embrapa Middle North, Teresina, Piauí State, Brazil,  from October 2015 to September 2016. The experiment was designed in a randomized block design in subdivided plots, with four replications. The plots consisted of four water regimes (irrigation plus rainfall) based on crop evapotranspiration fractions (50% ETc = 1,374.3 mm, 80% ETc = 1,534.2 mm, 110% ETc = 1,712.0 mm and 150% ETc = 1,905.8 mm) and the subplots per ten varieties of sugarcane (RBUFRPE01, RBUFRPE02, RBUFRPE03, RBUFV01, RB036066, RB987935, RB935744, RB943538, RB92579 and RB867515). The technological quality characteristics of sugarcane juice and yields of sugar and alcohol were affected by water regimes and varieties. The highest contents of Brix (22.5%), Fibers (13.3%), Pol-juice (17.5%) and Pol-stalk (13.9%) were obtained with varieties RB867515, RB935744, RB943538 and RB935744, respectively. The maxim yields of sugar (32.2 Mg ha-1) and alcohol (23 m3 ha-1) were obtained with  variety RB935744 and water replenishment close to 110% of the ETc.  Keywords: Saccharum officinarum, water efficiency, irrigation management.


Irriga ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 162-176
Author(s):  
Eduardo Magno Pereira da Silva ◽  
Aderson Soares de Andrade Júnior ◽  
Edson Alves Bastos ◽  
Valdenir Queiroz Ribeiro

PRODUTIVIDADE DE COLMOS E EFICIÊNCIA DO USO DA ÁGUA EM CANA-DE-AÇÚCAR IRRIGADA POR GOTEJAMENTO SUBSUPERFICIAL     EDUARDO MAGNO PEREIRA DA SILVA1; ADERSON SOARES DE ANDRADE JÚNIOR2; EDSON ALVES BASTOS2 E VALDENIR QUEIROZ RIBEIRO2 1Universidade Federal do Piauí (UFPI), Centro de Ciências Agrárias, Programa de Pós‑Graduação em Agronomia, Campus Socopo, Rua Dirce Oliveira, S/N, Ininga, CEP 64048‑550 Teresina, PI, Brasil. e-mail: [email protected]  2Embrapa Meio‑Norte, Avenida Duque de Caxias, n° 5.650, Bairro Buenos Aires, CEP 64006‑220 Teresina, PI. Brasil, e-mail: [email protected], [email protected], [email protected]     1 RESUMO   O objetivo desta pesquisa foi avaliar a produtividade de colmos e a eficiência de uso da água de variedades de cana-de-açúcar (cana planta) em resposta a diferentes regimes hídricos aplicados por gotejamento subsuperficial. O experimento foi realizado em um Argissolo Vermelho Amarelo distrófico, na Embrapa Meio-Norte, em Teresina, Piauí, Brasil, durante o período de outubro de 2015 a setembro de 2016. Utilizou-se o delineamento experimental de blocos casualizados em parcelas subdivididas, com quatro repetições. As parcelas foram constituídas por quatro regimes hídricos (irrigação mais precipitação pluviométrica) baseados em frações da evapotranspiração de cultura (50% ETc = 1.374,3; 80% ETc = 1.534,2; 110% ETc = 1.712,0 e 150% ETc = 1.905,8 mm) e as subparcelas por dez variedades de cana-de-açúcar (RBUFRPE01, RBUFRPE02, RBUFRPE03, RBUFV01, RB036066, RB987935, RB935744, RB943538, RB92579 e RB867515). A variação do regime hídrico promoveu alteração na performance produtiva e na eficiência do uso da água. A máxima produtividade de colmos (222,9 Mg ha-1) foi obtida com a variedade RB935744 e a lâmina total aplicada de 1.688,8 mm. A maior eficiência do uso da água (13,5 kg m-3) foi obtida com a variedade RB935744, aplicando-se lâmina total de 1.606,2 mm.   Palavras-chave: Saccharum officinarum, performance produtiva, planejamento de irrigação.     SILVA, E. M. P.; ANDRADE JÚNIOR, A. S.; BASTOS, E. A.; RIBEIRO, V. Q.STALKS YIELD AND WATER USE EFFICIENCY OF SUGARCANE IRRIGATED BY SUBSURFACE DRIPPING   2 ABSTRACT   This study aimed to evaluate stalks yield and water use efficiency of sugarcane varieties (cane plant) in response to different water regimes applied by subsurface drip irrigation. The experiment was carried out in a dystrophic Yellow Red Argisol at Embrapa Middle North, Teresina, Piauí State, Brazil, during the period from October 2015 to September 2016. The experiment was designed in a randomized block design in subdivided plots, with four replications. The plots consisted of four water regimes (irrigation plus rainfall) based on culture evapotranspiration fractions (50% ETc = 1,374.3 mm, 80% ETc = 1,534.2 mm, 110% ETc = 1,712.0 mm and 150% ETc = 1,905.8 mm) and subplots per ten varieties of sugarcane (RBUFRPE01, RBUFRPE02, RBUFRPE03, RBUFV01, RB036066, RB987935, RB935744, RB943538, RB92579 and RB867515). The variation of the water regime promoted a change in productive performance and water use efficiency. The maximum stalks yield (222.9 Mg ha-1) was obtained with variety RB935744 and the total applied depth of 1,688.8 mm. The greater efficiency of water use (13.5 kg m-3) was obtained with  variety RB935744, applying total depth of 1,606.2 mm.   Keywords: Saccharum officinarum, productive performance, irrigation planning.


2006 ◽  
Vol 84 (11) ◽  
pp. 1629-1633 ◽  
Author(s):  
Taylor A. Steeves

Although much of the current investigation of shoot apical meristems is in the realm of molecular genetic analysis, it is important that previous structural and functional studies not be overlooked as essential background to these studies. Since Caspar Friedrich Wolff described the shoot apical meristem in 1759, many and varied interpretations have arisen. In the early 19th century, the apical cell was recognized in seedless vascular plants and this interpretation was extended to seed plants. However, by the 1860s, this view was replaced in seed plants by the histogen concept, which recognized meristem layers in the apical meristem giving rise to specific tissues. In 1924, the tunica–corpus interpretation of angiosperm shoot apices became widespread, the two regions being distinguished by different planes of cell division. In the 1950s, the “méristème d’attente” concept appeared in France, which argued that the central region of the apex remained essentially inactive until the onset of flowering. Meanwhile, the recognition of zonation patterns in angiosperm and gymnosperm shoot apices assumed growing functional importance. Clonal analysis based on chimeras in the meristem indicated the presence of initial cells but also their replacement. Surgical experimentation and culture of excised apices in vitro stressed the autonomy of the shoot apex and its role in shoot development. Present molecular genetic analysis may help to resolve some of the persistent questions concerning the organization and functioning of shoot apical meristems.


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