IMMUNE PROPERTIES IN THE PRODUCTED RECOMBINANT SIP YOUNG 6 HIS PROTEIN FROM THE SEA STAR IGKAPPA GENE THROUGH HEK 293 EBNA CELLS REVEALING AGAINST SPIKE-RBD PROTEIN AND HRP ONE CHANGES IN SEA STAR GENE EXPRESSION?

2021 ◽  
pp. 28-32
Keyword(s):  
Gene Expression ◽  
Sea Star ◽  
Hek 293 ◽  
Star Gene

scholarly journals DAX-1 Expression in Human Adrenocortical Neoplasms: Implications for Steroidogenesis

1998 ◽  
Vol 83 (7) ◽  
pp. 2597-2600 ◽  
Author(s):  
M. Reincke ◽  
F. Beuschlein ◽  
E. Lalli ◽  
W. Arlt ◽  
S. Vay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Protein Gene ◽  
Regulatory Protein ◽  
Nuclear Hormone Receptor ◽  
Adrenocortical Tumors ◽  
Steroidogenic Acute Regulatory ◽  
Adrenocortical Carcinomas ◽  
Star Gene ◽  
Functional Phenotype

The DAX-1 gene encodes an orphan nuclear hormone receptor essential for normal fetal development of the adrenal cortex. Recently, DAX-1 has been shown to act as a transcriptional repressor of steroidogenic acute regulatory protein gene expression (StAR), suppressing steroidogenesis. We, therefore, investigated the expression of DAX-1 in a variety of adrenocortical tumors and compared the results with StAR mRNA expression. We found low or absent DAX-1 expression in aldosterone-producing adenomas (n=11: 35±11%; normal adrenals: 100±17%) and in aldosterone-producing adrenocortical carcinomas (n=2: 24 and 36%). Cortisol-producing adenomas showed intermediate DAX-1 expression (n=8; 92±16), as did 3 non-aldosterone-producing carcinomas (72, 132 and 132%). High DAX-1 expression was present in nonfunctional adenomas (n=3; 160±17%). In contrast to DAX-1, StAR mRNA expression did not show significant variations between groups. We did not detect the expected negative correlation between DAX-1 and StAR mRNA in adrenocortical tumors. These data suggest that high DAX-1 expression in adrenocortical tumors is associated with a non-functional phenotype whereas low DAX-1 expression favors mineralo-corticoid secretion. These effects on steroidogenesis are mediated by mechanisms other than repression of StAR gene expression. Our results indicate that DAX-1 may be one of the factors influencing the steroid biosynthesis of adrenocortical neoplasms.

scholarly journals Inhibition of Cyclooxygenase-2 Activity Enhances Steroidogenesis and Steroidogenic Acute Regulatory Gene Expression in MA-10 Mouse Leydig Cells

Endocrinology ◽  
2003 ◽  
Vol 144 (8) ◽  
pp. 3368-3375 ◽  
Author(s):  
XingJia Wang ◽  
Matthew T. Dyson ◽  
Youngah Jo ◽  
Douglas M. Stocco
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Leydig Cells ◽  
Promoter Activity ◽  
Regulatory Gene ◽  
Steroid Production ◽  
Star Protein ◽  
Cox Inhibitor ◽  
Steroidogenic Acute Regulatory ◽  
Star Gene

Abstract To study the mechanism for the regulatory effect of arachidonic acid (AA) on steroidogenesis, the role of cyclooxygenase (COX) in steroid production and steroidogenic acute regulatory (StAR) gene expression was investigated. Although stimulation with 0.05 mm dibutyryl cAMP (Bt2cAMP) did not increase StAR protein or progesterone in MA-10 mouse Leydig cells, the addition of 1 μm of the COX inhibitor indomethacin increased StAR protein expression and progesterone production by 5.7-fold and 34.3-fold, respectively. In the presence of indomethacin, the level of Bt2cAMP required for maximal steroidogenesis was reduced from 1.0 mm to 0.25 mm. Similar results were obtained in studies on StAR promoter activity and in Northern blot analyses of StAR mRNA expression, suggesting that inhibition of COX activity enhanced StAR gene transcription. COX2 (an inducible isoform of COX) was constitutively detected in MA-10 cells. Although SC560, a selective COX1 inhibitor, did not affect steroidogenesis, the COX2 inhibitor NS398 significantly enhanced Bt2cAMP-stimulated StAR protein expression and steroid production. Overexpression of the COX2 gene in COS-1 cells significantly inhibited StAR promoter activity. The results of the present study suggest that inhibition of COX2 activity increases the sensitivity of steroidogenesis to cAMP stimulation in MA-10 Leydig cells.

scholarly journals Involvement of the Thromboxane A2 Receptor in the Regulation of Steroidogenic Acute Regulatory Gene Expression in Murine Leydig Cells

Endocrinology ◽  
2009 ◽  
Vol 150 (7) ◽  
pp. 3267-3273 ◽  
Author(s):  
Akhilesh K. Pandey ◽  
Xiangling Yin ◽  
Randolph B. Schiffer ◽  
James C. Hutson ◽  
Douglas M. Stocco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Leydig Cells ◽  
Regulatory Gene ◽  
Thromboxane A2 ◽  
Cyclooxygenase 2 ◽  
Thromboxane A2 Receptor ◽  
Star Protein ◽  
Dependent Inhibition ◽  
Steroidogenic Acute Regulatory ◽  
Star Gene

Recent studies suggested an involvement of thromboxane A2 in cyclooxygenase-2-dependent inhibition of steroidogenic acute regulatory (StAR) gene expression. The present study further investigated the role of thromboxane A2 receptor in StAR gene expression and steroidogenesis in testicular Leydig cells. The thromboxane A2 receptor was detected in several Leydig cell lines. Blocking thromboxane A2 binding to the receptor using specific antagonist SQ29548 or BM567 resulted in dose-dependent increases in StAR protein and steroid production in MA-10 mouse Leydig cells. The results were confirmed with Leydig cells isolated from rats. StAR promoter activity and StAR mRNA level in the cells were also increased after the treatments, suggesting an involvement of the thromboxane A2 receptor in StAR gene transcription. Furthermore study indicated that blocking the thromboxane A2 receptor reduced dosage sensitive sex reversal-adrenal hypoplasia congenita critical region on the X chromosome, gene 1 protein, a transcriptional repressor of StAR gene expression. Specific binding of the antagonists to the receptors on cellular membrane was demonstrated by binding assays using 3H-SQ29548 and binding competition between 3H-SQ29548 and BM567. Whereas SQ29548 enhanced cAMP-induced StAR gene expression, in the absence of cAMP, it was unable to increase StAR protein and steroidogenesis. However, when the receptor was blocked by the antagonist, subthreshold levels of cAMP were able to induce maximal levels of StAR protein expression, suggesting that blocking the thromboxane A2 receptor increase sensitivity of MA-10 cells to cAMP stimulation. Taken together, the results from the present and previous studies suggest an autocrine loop, involving cyclooxygenase-2, thromboxane A synthase, and thromboxane A2 and its receptor, in cyclooxygenase-2-dependent inhibition of StAR gene expression.

scholarly journals Porcine and Bovine Steroidogenic Acute Regulatory Protein (StAR) Gene Expression during Gestation1

Endocrinology ◽  
1997 ◽  
Vol 138 (3) ◽  
pp. 1085-1091 ◽  
Author(s):  
Nicolas Pilon ◽  
Isabelle Daneau ◽  
Chantal Brisson ◽  
Jean-François Ethier ◽  
Jacques G. Lussier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Protein ◽  
Steroidogenic Acute Regulatory Protein ◽  
Steroidogenic Acute Regulatory ◽  
Star Gene

Effects of Scutellariae Radix on gene expression in HEK 293 cells using cDNA microarray

2006 ◽  
Vol 105 (3) ◽  
pp. 346-351 ◽  
Author(s):  
Chia-Sheng Chen ◽  
Nae-Jing Chen ◽  
Li-Wei Lin ◽  
Chia-Chang Hsieh ◽  
Guang-Wei Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Microarray ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
Scutellariae Radix ◽  
293 Cells

scholarly journals Interaction between arachidonic acid and cAMP signaling pathways enhances steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells

2002 ◽  
Vol 188 (1-2) ◽  
pp. 55-63 ◽  
Author(s):  
Xing Jia Wang ◽  
Matthew T. Dyson ◽  
Carolina Mondillo ◽  
Zoraida Patrignani ◽  
Omar Pignataro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arachidonic Acid ◽  
Signaling Pathways ◽  
Tumor Cells ◽  
Camp Signaling ◽  
Leydig Tumor Cells ◽  
Star Gene

scholarly journals Recombinant Sip Young 6 His Protein Production from the Sea Star Igkappa Gene, after Cloning

2021 ◽  
Vol 2 (2) ◽  
pp. 85-86
Author(s):  
Michel Leclerc
Keyword(s):  
Protein Production ◽  
Sea Star ◽  
Hek 293 ◽  
First Time

In 2014 we have isolated and cloned the sea star Igkappa gene which induced an anti-HRP primitive invertebrate antibody. We attempt, in the present work, for the first time in Invertebrates, to produce a SIP Young 6 His protein through HeK 293 EBNA cells.

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