Comparison of Sensitivity Enterobacteriaceae of Extended Spectrum BetaLactamase (ESBL) against Antibiotics of Quinolone and Carbapenem Group in Clinical Microbiology Laboratory, Faculty of Medicine, Universitas Indonesia

2020 ◽  
Vol 4 (2) ◽  
pp. 71-76
Author(s):  
Conny Riana Tjampakasari ◽  
Alya Iranti ◽  
Tjahjani Mirawati Sudiro
Keyword(s):  
Clinical Microbiology ◽  
Antibiotic Sensitivity ◽  
Female Gender ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Beta Lactam ◽  
Medical Problems ◽  
E Coli ◽  
Beta Lactam Antibiotics ◽  
Age Range

Antibiotic resistance is a challenge in medical problems. One prevalence of resistance that tends to expand globally is against ESBL-producing Enterobacteriaceae, a group of bacteria capable of destroying beta-lactam antibiotics. The known ESBL producing bacteria are E. coli and K. pneumoniae. This study aims to compare the sensitivity of quinolone and carbapenem antibiotics to ESBL-producing bacteria based on data obtained from Clinical Microbiology Laboratory, Faculty of Medicine, Universitas Indonesia through 2018-2019. Using the Vitek 2® Compact identification method, the results showed that the prevalence of E. coli and K. pneumoniae ESBL was positive less than 5%. All of the ESBL–producing E. coli came from urine specimens, while ESBLproducing K. pneumoniae came from different types of specimens which are sputum and blood. Most prevalence comes in the age range >50 years with female gender. In general, antibiotic sensitivity to the quinolones was less than 50% against ESBL-producing E. coli. Meanwhile, the sensitivity of carbapenem antibiotics reached 100% both against ESBL-producing E.coli and K.pneumoniae.

Simplified scheme for identification of prompt lactose-fermenting members of the Enterobacteriaceae

1976 ◽  
Vol 4 (6) ◽  
pp. 511-514
Author(s):  
M J Hicks ◽  
K J Ryan
Keyword(s):  
Escherichia Coli ◽  
Ornithine Decarboxylase ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Biochemical Tests ◽  
Gram Negative ◽  
E Coli ◽  
Colonial Morphology ◽  
Level Identification

A brief, simplified scheme involving the spot indole test and colonial morphology was evaluated for genus level identification of prompt lactose-fermenting (PLF) members of the Enterobacteriaceae. One hundred and ninety-four consecutive, clinically important PLF gram-negative rods isolated in a clinical microbiology laboratory were identified by this simplified scheme, as well as by standard biochemical tests, and the API 20E (Analytab Products, Inc., Plainview, N.Y.) system. In the simplified scheme a flat, spot indole-positive colony was identified as Escherichia coli. Spot indole-negative organisms forming nucoid colonies were identified as Klebsiella sp. or Enterobacter sp. on the basis of semisolid motility and ornithine decarboxylase tests. Approximately 94% of the study isolates followed reactions typical for E. coli, Klebsiella sp., and Enterobacter sp. as defined by this simplified scheme. When compared with the standard and Analytab Products Inc. identifications, the overall accuracy was 97.4%. The accuracy of identification of E. coli, Klebsiella sp., and Enterobacter sp. was 98.1%, 95.6%, and 87.5%, respectively. This simplified scheme is recommended for identification of selected PLF isolates in the clinical microbiology laboratory.

scholarly journals DETEKSI GEN SHV PADA ISOLAT KLINIK Escherichia coli PENGHASIL EXTENDED SPECTRUM BETA−LACTAMASES (ESBLs) DENGAN METODE POLYMERASE CHAIN REACTION (PCR) DARI URIN PASIEN DI RSUD Dr. SOETOMO SURABAYA

2018 ◽  
Vol 11 (2) ◽  
pp. 91-98
Author(s):  
Yulianto Ade Prasetya
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Clinical Microbiology Laboratory ◽  
Beta Lactam ◽  
Chain Reaction ◽  
E Coli ◽  
Beta Lactam Antibiotics ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
Polymerase Chain

 AbstrakEscherichia coli penghasil Extended Spectrum Beta Lactmases (ESBLs) bertanggungjawab terhadap terjadinya wabah infeksi nosokomial, peningkatan morbiditas dan mortalitas, serta peningkatan biaya kesehatan. Enzim yang diproduksi oleh gen SHV dari bakteri mampu menghidrolisis antibiotik sefotaksim dan seftazidim. Tujuan penelitian ini adalah untuk mengetahui prevalensi gen SHV pada isolat klinik E. coli penghasil ESBLs dari urin pasien yang merupakan koleksi Laboratorium Mikrobiologi Klinik RSUD Dr. Soetomo Surabaya pada bulan Januari-Februari 2014. Jenis penelitian yang digunakan adalah observasional deskriptif dengan pendekatan molekuler. Deteksi gen SHV menggunakan metode Polymerase Chain Reaction (PCR) yang kemudian dilakukan elektroforesis dan divisualisasikan pada gel agarose 1,5%. Isolat E. coli yang positif membawa gen SHV ditunjukkan dengan adanya amplikon sebesar 867 bp. Hasil penelitian menunjukkan bahwa dari 30 isolat, sebanyak 12 isolat (40%) positif mengandung gen SHV, dengan prevalensi tertinggi berada di Ruang Instalasi Rawat Jalan. Meropenem dan fosfomisin masih dapat digunakan untuk terapi penyakit yang disebabkan oleh E. coli penghasil ESBLs. Deteksi ESBLs secara genotipik penting dilakukan karena beberapa gen ESBLs menunjukkan resistensi yang berbeda terhadap antibiotik golongan beta laktam. Hasil tersebut memberi informasi kepada pihak rumah sakit manapun untuk mewaspadai prevalensi E. coli penghasil ESBLs melalui pengawasan yang ketat pelaksanaan pemberian antibiotika sesuai tata laksananya.Abstract Extended Spectrum Beta Lactmases (ESBLs) producing−Escherichia coli strains are responsible for the occurrence of nosocomial infection outbreaks, increase of morbidity and mortality, as well as increased healthcare costs. The enzyme produced by the SHV gene from bacteria are able to hydrolyze antibiotics of cefotaxime and cefazazim. The purpose of this study was to detect the presence of SHV gene in clinical ESBLs−producing E. coli isolates from patients’ urines which are collections of Clinical Microbiology Laboratory in Dr. Soetomo Hospital of Surabaya within period of January−February 2014. The research used descriptive observasional design with molecular approach. The SHV gene was detected by using Polymerase Chain Reaction (PCR) method, then the products was performed by electrophoresis and visualized on 1.5% agarose gel. E. coli isolates that positively carrying the SHV gene were demonstrated in the presence of amplicons of 867 bp. The results showed that of 30 isolates, 12 isolates (40%) positively contained the SHV gene, with the highest prevalence being in the Outpatient Installation Room. Meropenem and fosfomycin can still be used for disease therapy caused by ESBLs−producing E. coli. Genotypic detection of ESBLs is important because some ESBLs genes exhibit different resistance to beta−lactam antibiotics. The result provides information to any hospital to be aware of the prevalence of ESBLs−producing E.coli through strict supervision of the implementation of antibiotics according to their administration.

scholarly journals Decrease in Enteroviral Meningitis - An Unexpected Benefit of COVID-19 Mitigation?

2020 ◽  
Author(s):  
Kami D Kies ◽  
Amber S Thomas ◽  
Matthew J Binnicker ◽  
Kelli L Bashynski ◽  
Robin Patel
Keyword(s):  
Cerebrospinal Fluid ◽  
Laboratory Testing ◽  
Clinical Microbiology ◽  
Late Summer ◽  
Mitigation Strategies ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Testing Data ◽  
Early Fall ◽  
Enteroviral Meningitis

Abstract Enteroviral meningitis is seasonal, typically exhibiting a rise in prevalence in late summer/early fall. Based on clinical microbiology laboratory testing data of cerebrospinal fluid, the expected August/September/October peak in enteroviral meningitis did not occur in 2020, possibly related to COVID-19 mitigation strategies.

scholarly journals Implementation and evaluation of a novel real-time multiplex assay for SARS-CoV-2: in-field learnings from a clinical microbiology laboratory

Pathology ◽  
2020 ◽  
Vol 52 (7) ◽  
pp. 754-759 ◽  
Author(s):  
Eloise Williams ◽  
Katherine Bond ◽  
Brian Chong ◽  
Dawn Giltrap ◽  
Malcolm Eaton ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Microbiology ◽  
Multiplex Assay ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory

scholarly journals One Small Step for the Gram Stain, One Giant Leap for Clinical Microbiology

2016 ◽  
Vol 54 (6) ◽  
pp. 1416-1417 ◽  
Author(s):  
Richard B. Thomson
Keyword(s):  
Clinical Microbiology ◽  
Rapid Test ◽  
Error Rates ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Gram Stain ◽  
Small Step ◽  
Link Type ◽  
Giant Leap

The Gram stain is one of the most commonly performed tests in the clinical microbiology laboratory, yet it is poorly controlled and lacks standardization. It was once the best rapid test in microbiology, but it is no longer trusted by many clinicians. The publication by Samuel et al. (J. Clin. Microbiol. 54:1442–1447, 2016,http://dx.doi.org/10.1128/JCM.03066-15) is a start for those who want to evaluate and improve Gram stain performance. In an age of emerging rapid molecular results, is the Gram stain still relevant? How should clinical microbiologists respond to the call to reduce Gram stain error rates?

scholarly journals Demonstration of Cord Formation by Rough Mycobacterium abscessus Variants: Implications for the Clinical Microbiology Laboratory: Fig. 1.

2011 ◽  
Vol 49 (6) ◽  
pp. 2293-2295 ◽  
Author(s):  
Alejandro Sánchez-Chardi ◽  
Francesc Olivares ◽  
Thomas F. Byrd ◽  
Esther Julián ◽  
Cecilia Brambilla ◽  
...  
Keyword(s):  
Clinical Microbiology ◽  
Mycobacterium Abscessus ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory
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