Immunohistochemical expression of MUC4 in different grades of Head and Neck Squamous cell carcinoma

2020 ◽  
pp. 1-14
Author(s):  
Naila Umer ◽  
Nadia Naseem ◽  
Saima Chaudhry
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Oral Pathology ◽  
Neck Squamous Cell Carcinoma ◽  
Urban Setting ◽  
Immunohistochemical Expression ◽  
Normal Epithelium

Abstract Objective: To determine immunohistochemical expression of Mucin 4 in head and neck squamous cell carcinoma and its different histological grades among patients reporting to various tertiary care hospitals in an urban setting. Method: The descriptive study was conducted at the Department of Oral Pathology / Morbid Anatomy and Histopathology, University of Health Sciences, Lahore, Pakistan, from January to July 2017, and comprised cases of head and neck squamous cell carcinoma. Histological diagnosis and grading was done for each case. Haematoxylin and eosin stain followed by immunohistochemistry was done. Relation of Mucin 4 expression with tumour types was explored. SPSS 20 was used for statistical analysis. Result: Of the 63 samples, 40(63.5%) were from male patients. The overall mean age of the patients was 53±3.77 years. Mucin 4 expression was positive in 47(74.6%) cases. Of them, 16(34%) had grade 1 tumour, 28(59.6%) had grade 2 and 3(6.4%) had grade 3 tumour. tumourThere was a significant relation (p=0.03) between tumour grades and intensity of Mucin 4 expression. tumour Conclusion: Upregulation of Mucin 4in tumour tissue with no expression in normal epithelium was found, and loss of Mucin 4 expression with increase in tumour grade was noted. Key Words: Mucin 4, MUC4, Squamous cell carcinoma of head and neck, HNSCC, Immunohistochemistry Continuous...

Immunohistochemical expression of p53 and MDM2 within head and neck squamous cell carcinoma

2006 ◽  
Vol 31 (3) ◽  
pp. 247-247 ◽  
Author(s):  
S. Tandon ◽  
H. Beer ◽  
T.M. Jones ◽  
M. Birchall ◽  
M. Boyd ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Immunohistochemical Expression

Distant Metastases and Survival Prediction in Head and Neck Squamous Cell Carcinoma

2012 ◽  
Vol 147 (5) ◽  
pp. 870-875 ◽  
Author(s):  
Dong Hwan Lee ◽  
Min Joo Kim ◽  
Jong-Lyel Roh ◽  
Sung-Bae Kim ◽  
Seung-Ho Choi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Multivariate Analyses ◽  
Tertiary Care ◽  
Distant Metastases ◽  
Neck Squamous Cell Carcinoma ◽  
Survival Prediction

Objective This study was designed to identify the risk factors associated with distant metastases (DM) and survival outcomes in patients with head and neck squamous cell carcinoma (HNSCC). Study Design Database analysis plus chart review. Setting Tertiary care academic medical center. Subjects and Methods Four hundred four eligible patients were involved who were treated with a minimum 2-year follow-up from January 2005 through August 2009. Confirmation of DM was performed by histopathology or follow-up imaging. Univariate and multivariate analyses were used for factors predictive of DM. The survival of patients was calculated by the Kaplan-Meier method. Results Thirty-six of the 404 eligible patients (8.9%) developed DM, most frequently in the lungs (80.6%). Univariate analyses revealed that T and N classifications, lymphovascular invasion and perineural invasion of the primary tumor, and locoregional recurrences were each significantly associated with the development of DM ( P < .05). Multivariate analyses showed that T and N classifications and locoregional recurrences were independent predictors of DM ( P < .05). The overall 1- and 2-year survival rates of the 36 patients with DMs were 40.6% and 13.5%, respectively. Metastases to more than 1 organ negatively affected patient survival ( P < .001). Conclusion Patients with advanced tumors, nodal involvement, or locoregional recurrences may require more frequent surveillance for the development of DMs.

scholarly journals CORRELATION OF P21 EXPRESSION IN HEAD AND NECK SQUAMOUS CELL CARCINOMA WITH CLINICOPATHOLOGIC AND PROGNOSTIC PARAMETERS

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Safana Sadaf ◽  
Asif Loya ◽  
Sajid Mushtaq ◽  
Noreen Akhter ◽  
Raza Hussain ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Nodal Metastasis ◽  
Neck Squamous Cell Carcinoma ◽  
Depth Of Invasion ◽  
Increased Risk

Purpose: Head and neck tumours include tumours of nose, paranasal sinuses, oropharynx, larynx and mouth. Squamous cell carcinoma (SCC) is the most common head and neck malignant tumour which accounts for 90% of head and neck malignant tumours. p21 is an important immunohistochemical marker which has signi cant role in predicting prognosis of head and neck SCC (HNSCC). Increased p21 expression in HNSCC is associated with bad prognosis in terms of increased risk of nodal metastasis, increased rate of recurrence and decreased survival rate. The purpose of this study was to evaluate the expression of p21 in HNSCC with various clinicopathologic and prognostic parametres. Materials and Methods: A total of 110 patients (n = 110) of HNSCC (oral cavity n = 98 and laryngeal tumours n = 12) were included in the study which were diagnosed and treated between January 2008 and January 2011 at a tertiary care centre in Pakistan. Mean age was 51 years (age range 15–78). 65 (59%) were male and 45 (41%) were female. Tumours were classi ed as p21 positive when ≥10% tumour cells were immunoreactive for p21. p21 expression was noted and correlated with T-stage, nodal metastasis, perineural and depth of invasion, recurrence and 2-year survival rate. Results: Of 110 cases, p21-positive cases were 88 (80%) compared to 22 (20%) negative. T1–T2 tumours with p21 expression were 59 (67.04%) while 17 cases (77.27%) had negative for p21 (P = 0.44). Amongst T3–T4, 29 cases showed p21 expression (32.96%) while 5 cases (22.73%) were negative (P = 0.44). Nodal metastasis was seen in 51 cases (57.95%) with p21 positivity as compared to 4 cases (18.8%) with no p21 expression (P = 0.0015). Perineural invasion was seen in 15 carcinomas (17.04%) having p21 positivity and 1 case (4.54%) with p21 negativity (P = 0.18). 48 cases (54.54%) had depth of invasion >1 cm with positive p21 as compared to 4 cases (18.18%) without (P value = 0.0035). 39 cases (44.31%) showed recurrence along with p21 expression while 3 cases (13.63%) showed recurrence without (P = 0.0076). 2-year survival rate was 56.81% (n = 88) in p21 positive cases, whereas it was 90.90% (n = 20) those with negative p21 (P = 0.0026). Conclusion: Positive p21 expression in HNSCC correlates with intermediate grade, late stage, increased nodal metastasis, tumour recurrence and decreased survival. p21 should be considered as an important prognostic and predictive marker in HNSCC to detect tumours at early stage and to improve therapy and prognosis. Key words: Head and neck squamous cell carcinoma, lymph node metastasis, p21, recurrence, survival rate 

Loss of CD169+ Subcapsular Macrophages during Metastatic Spread of Head and Neck Squamous Cell Carcinoma

2019 ◽  
Vol 161 (1) ◽  
pp. 67-73
Author(s):  
Michael C. Topf ◽  
Larry Harshyne ◽  
Madalina Tuluc ◽  
Stacey Mardekian ◽  
Swar Vimawala ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Nodes ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Care Center ◽  
Tertiary Care ◽  
Neck Squamous Cell Carcinoma ◽  
Metastatic Spread ◽  
Regional Lymph Nodes

Objective The purpose of this study is to assess CD169 expression in metastatic and nearby tumor-free lymph nodes of patients with head and neck squamous cell carcinoma (SCC). Study Design Retrospective analysis based on immunohistochemistry. Setting Tertiary care center. Subjects and Methods The abundance of CD169+ cells in the subcapsular sinuses (SCSs) of lymph nodes was assessed immunohistochemically in paraffin-embedded tissue samples derived from 22 patients with oral cavity and oropharyngeal SCC. Results SCSs of lymph nodes harboring metastatic SCC contained significantly fewer CD169+ macrophages (106.5 ± 113.6 cells/mm2) compared to nearby tumor-free lymph nodes (321.3 ± 173.4 cells/mm2, P < .001). This observation extended to 21 of the 22 cases investigated. In addition, 6 patients who later developed recurrent disease contained lower numbers of CD169+ cells (268.6 ± 169.5 cells/mm2) in nearby tumor-free lymph nodes compared to 341.0 ± 176.1 cells/mm2 in those who remained disease free ( P = .399). Human papillomavirus (HPV)–positive patients (n = 4) had a 6-fold lower number of CD169+ cells in metastatic nodes (61.2 ± 85.5 cells/mm2) compared to nearby tumor-free lymph nodes (369.5 ± 175.5 cells/mm2, P = .028). In comparison, HPV-negative patients had only a 3-fold reduction (116.6 ± 118.5 cells/mm2 vs 310.6 ± 176.2 cells/mm2, P < .001). Conclusion Metastatic spread of SCC to regional lymph nodes is associated with lower abundance of CD169+ macrophages in the SCSs of draining lymph nodes. These results set the stage for an in-depth investigation into the mechanism(s) by which metastatic SCC controls CD169+ macrophage abundance and its significance as it relates to prognosis and treatment response.

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