Where Does Current Quorum Sensing Research Stand

2020 ◽  
Author(s):  
Spring Library
Keyword(s):  
Enzyme Activity ◽  
Quorum Sensing ◽  
Quorum Quenching ◽  
Signalling Molecules ◽  
Quorum Sensing Signals

Quorum quenching is achieved by inactivating signalling enzymes, by introducing molecules that mimic signalling molecules and block their receptors, by degrading signalling molecules themselves, or by a modification of the quorum sensing signals due to an enzyme activity.

scholarly journals Engineering quorum quenching enzymes: progress and perspectives

2019 ◽  
Vol 47 (3) ◽  
pp. 793-800 ◽  
Author(s):  
Shereen A. Murugayah ◽  
Monica L. Gerth
Keyword(s):  
Quorum Sensing ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Microbial Pathogens ◽  
Human Pathogens ◽  
New Approach ◽  
Signalling Molecules ◽  
The Stability

Abstract Quorum sensing is a key contributor to the virulence of many important plant, animal and human pathogens. The disruption of this signalling—a process referred to as ‘quorum quenching’—is a promising new approach for controlling microbial pathogens. In this mini-review, we have focused on efforts to engineer enzymes that disrupt quorum sensing by inactivating acyl-homoserine lactone signalling molecules. We review different approaches for protein engineering and provide examples of how these engineering approaches have been used to tailor the stability, specificity and activities of quorum quenching enzymes. Finally, we grapple with some of the issues around these approaches—including the disconnect between in vitro biochemistry and potential in vivo applications.

scholarly journals Characterization of a Phosphotriesterase-Like Lactonase fromSulfolobus solfataricusand Its Immobilization for Disruption of Quorum Sensing

2010 ◽  
Vol 77 (4) ◽  
pp. 1181-1186 ◽  
Author(s):  
Filomena S. W. Ng ◽  
Daniel M. Wright ◽  
Stephen Y. K. Seah
Keyword(s):  
Quorum Sensing ◽  
Virulence Factors ◽  
Acyl Chain ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Homoserine Lactones ◽  
Specificity Constant ◽  
Chain Lengths ◽  
Quorum Sensing Signals

ABSTRACTSsoPox, a bifunctional enzyme with organophosphate hydrolase andN-acyl homoserine lactonase activities from the hyperthermophilic archaeonSulfolobus solfataricus, was overexpressed and purified from recombinantPseudomonas putidaKT2440 with a yield of 9.4 mg of protein per liter of culture. The enzyme has a preference forN-acyl homoserine lactones (AHLs) with acyl chain lengths of at least 8 carbon atoms, mainly due to lowerKmvalues for these substrates. The highest specificity constant obtained was forN-3-oxo-decanoyl homoserine lactone (kcat/Km= 5.5 × 103M−1·s−1), but SsoPox can also degradeN-butyryl homoserine lactone (C4-HSL) andN-oxo-dodecanoyl homoserine lactone (oxo-C12-HSL), which are important for quorum sensing in ourPseudomonas aeruginosamodel system. WhenP. aeruginosaPAO1 cultures were grown in the presence of SsoPox-immobilized membranes, the production of C4-HSL- and oxo-C12-HSL-regulated virulence factors, elastase, protease, and pyocyanin were significantly reduced. This is the first demonstration that immobilized quorum-quenching enzymes can be used to attenuate the production of virulence factors controlled by quorum-sensing signals.

scholarly journals Cyclic Dipeptides Produced by Marine Sponge-Associated Bacteria as Quorum Sensing Signals

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Gennaro Roberto Abbamondi ◽  
Salvatore De Rosa ◽  
Carmine Iodice ◽  
Giuseppina Tommonaro
Keyword(s):  
Quorum Sensing ◽  
Quorum Quenching ◽  
Marine Sponges ◽  
Bacterial Strains ◽  
Signal Molecule ◽  
Associated Bacteria ◽  
Cyclic Dipeptides ◽  
Sponge Associated Bacteria ◽  
Dysidea Avara ◽  
Quorum Sensing Signals

Four bacterial strains belonging to the genera Vibrio, Pseudoalteromonas and Photobacterium were isolated from the marine sponges Dysidea avara and Geodia cynodium. A Bacillus strain was isolated from Ircinia variabilis. A screening of molecules involved in quorum sensing (QS) was carried out by TLC-overlay and a new “plate T-streak” test. To analyze quorum quenching (QQ), a plate T-streak was performed with Chromobacterium violaceum. Strains of Vibrio isolated from both marine sponges and a strain of Photobacterium isolated from G. cynodium, activated QS bioreporters. A strain of Pseudoalteromonas isolated from D. avara showed QQ activity. Finally, it is reported that cyclic dipeptides isolated from strains of Vibrio sp. and Bacillus sp. (isolated from D. avara and I. variabilis, respectively) were involved in the QS mechanism. The simultaneous presence of bacteria that showed contrasting responses in bioassays for QS signal molecule synthesis in marine sponges could add an interesting dimension to the signalling interactions which may be happening in sponges.

Quorum Quenching for Sustainable Environment

2021 ◽  
pp. 542-568
Author(s):  
Sumira Malik ◽  
Shilpa Prasad ◽  
Tanvi Kumari ◽  
Shreya Ghoshal ◽  
Ankita Agrawal ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Quorum Quenching ◽  
Negative Regulation ◽  
Soil Reclamation ◽  
Bacterial Communication ◽  
Considerable Contribution ◽  
Signalling Molecules ◽  
Sustainable Environment ◽  
Microbial Systems ◽  
Signalling Cascade

Quorum quenching is the process that prevents quorum sensing through the disruption of signalling cascade and bacterial communication among themselves mediated by the degradation of the signalling molecules. Therefore, quorum quenching has a considerable contribution in the negative regulation of threatening diseases and eventually increasing soil reclamation through different mechanism mediated by microorganisms in reclamation of soil. Quorum sensing has a significant contribution in enhancement of soil quality through microbial-based enzymes and mechanism in the versatile fields which are a component of the environment. The current chapter discusses the details of various direct and indirect mechanisms mediated by microbial systems that have a significant role in soil reclamation for the sustenance of the environment.

Sign in / Sign up

Export Citation Format

Share Document