Anticancer and antioxidant activity of ethanolic extract of Clove  (Syzygium aromaticum) on oral squamous cell carcinoma cell lines  (KB cell lines)- An In-vitro study

Cloves (Syzygium aromaticum) as been used as traditional medicine for many years and they possess antibacterial, antifungal and antiviral properties. Clove is known for its anticancer property on various cancer cell lines and is well established, but its anticancer effect on OSCC cell lines is less known. Aim of the study was to determine the anticancer and antioxidant effect of Syzygium aromaticum extract on OSCC cell lines (KB cell lines) and compare the same with normal mouse fibroblasts cell lines (L292 cell lines). KB cell lines and L292 cell lines were commercially obtained. Clove was obtained from local market and ethanolic extract (EC) of clove was prepared. Anticancer activity was assessed by MTT, neutral red, DAPI and Double staining assay and antioxidant assay was carried out by FRAP, PM and DPPH assay. The antioxidant property of EC of clove increased with increase in the concentration in a dose dependent manner. Both MTT and Neutral Red assay showed increase in cell death with increase in concentration of EC of clove. Double staining and DAPI showed increase in cell death when treated with EC of clove. The anticancer and antioxidant activity of EC of clove was comparable with standard drug used in the assay. This in vitro study demonstrates effective anticancer and antioxidant activity on KB cell lines when compared to standard control. However, further studies are to be conducted in order to characterize other potential antitumor components of the clove, so that it can be used as therapeutic agent in treating oral carcinoma.

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Evaluation of anticancer and antioxidant activity of ethanolic extract of Ocimum sanctum on KB cell lines- an in-vitro study

Annals of Ayurvedic Medicine ◽

10.5455/aam.80349 ◽

2021 ◽

pp. 1

Author(s):

Renuka Ammanagi ◽

Vijayalakshmi Kotrashetti ◽

Vijay Kumbar ◽

Kishore Bhat ◽

Sheethal Sanikop ◽

...

Keyword(s):

Antioxidant Activity ◽

Cell Lines ◽

In Vitro Study ◽

Ethanolic Extract ◽

Vitro Study ◽

Ocimum Sanctum

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Syzygium aromaticum Extracts as a Potential Antibacterial Inhibitors against Clinical Isolates of Acinetobacter baumannii: An In-Silico-Supported In-Vitro Study

Antibiotics ◽

10.3390/antibiotics10091062 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1062

Author(s):

Abdelhamed Mahmoud ◽

Magdy M. Afifi ◽

Fareed El Shenawy ◽

Wesam Salem ◽

Basem H. Elesawy

Keyword(s):

Acinetobacter Baumannii ◽

In Silico ◽

In Vitro Study ◽

Ethanolic Extract ◽

Vitro Study ◽

Clinical Samples ◽

Syzygium Aromaticum ◽

Molecular Docking Study ◽

Spectra Analysis

Imipenem is the most efficient antibiotic against Acinetobacter baumannii infection, but new research has shown that the organism has also developed resistance to this agent. A. baumannii isolates from a total of 110 clinical samples were identified by multiplex PCR. The antibacterial activity of Syzygium aromaticum multiple extracts was assessed following the GC-Mass spectra analysis. The molecular docking study was performed to investigate the binding mode of interactions of guanosine (Ethanolic extract compound) against Penicillin- binding proteins 1 and 3 of A. baumannii. Ten isolates of A. baumannii were confirmed to carry recA and iutA genes. Isolates were multidrug-resistant containing blaTEM and BlaSHV. The concentrations (0.04 to 0.125 mg mL−1) of S. aromaticum ethanolic extract were very promising against A. baumannii isolates. Even though imipenem (0.02 mg mL−1) individually showed a great bactericidal efficacy against all isolates, the in-silico study of guanosine, apioline, eugenol, and elemicin showed acceptable fitting to the binding site of the A. baumannii PBP1 and/or PBP3 with highest binding energy for guanosine between −7.1 and −8.1 kcal/mol respectively. Moreover, it formed π-stacked interactions with the residue ARG76 at 4.14 and 5.6, Å respectively. These findings might support the in vitro study and show a substantial increase in binding affinity and enhanced physicochemical characteristics compared to imipenem.

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