Phylogenetic analysis of nucleoprotein gene of Rabies virus in Malaysia from 2015 to 2018

SUMMARYIn Chile, dog rabies has been controlled and insectivorous bats have been identified as the main rabies reservoir. This study aimed to determine the rabies virus (RABV) variants circulating in the country between 2002 and 2008. A total of 612 RABV isolates were tested using a panel with eight monoclonal antibodies against the viral nucleoprotein (N-mAbs) for antigenic typing, and a product of 320-bp of the nucleoprotein gene was sequenced from 99 isolates. Typing of the isolates revealed six different antigenic variants but phylogenetic analysis identified four clusters associated with four different bat species. Tadarida brasiliensis bats were confirmed as the main reservoir. This methodology identified several independent rabies enzootics maintained by different species of insectivorous bats in Chile.

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Genetic Mapping of Rabies Virus in Dogs as a Basis for Disease Control

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v10i1.3369 ◽

2016 ◽

Vol 10 (1) ◽

Author(s):

Muharam Saepulloh ◽

R. M. Abdul Adji

Keyword(s):

Phylogenetic Analysis ◽

Amino Acid ◽

Amino Acid Sequence ◽

Phylogenetic Relationship ◽

Rabies Virus ◽

Virus Isolate ◽

Protein Gene ◽

Genetic Characteristics ◽

Nucleoprotein Gene ◽

N Protein

The purpose of this study was to determine the genetic characteristics of rabies virus based on phylogenetic relationship among rabies virus in various regions in Indonesia. The amino acid sequence of the nucleoprotein gene of rabies virus isolate from Banten (RV/Banten01/dog/2007),Makasar (RV/MKS-26/dog/2010), Bukit Tinggi (RV/BKT-52/dog/2009 and RV/BKT-58/dog/2009), Medan (RV/Medan27/dog/2007)andBali(RV/Bali-1/dog/2009;RV/Bali-2/dog/2009;RV/Bali-3/dog/2009),Indonesiawasdetermined.TheseisolatesshowedahighdegreeofhomologyamongIndonesianisolateswhichreached100%.Meanwhile,thelevelofhomologybetweenrabiesvirusisolatesfromcatsrabiesvirusisolatesfromdogsreached97%.ResultsofphylogeneticanalysisusingtheaminoacidsequencesoftheNgenesshowedthatallofIndonesianrabiesvirusisolateswerecloselyrelatedtorabiesvirusesfromChinathanthosefromThailand,Laos,Burma,andVietnamwhichgeograficallycloser to Indonesia. Data obtained from the phylogenetic analysis is expected to trace the source of rabies spread and thepossibility to create a vaccines which more suitable with rabies virus that spreads in Indonesia. Based on the phylogenetic relationship analysisusing the amino acid sequence of the rabies virus N protein gene showed that all of rabies virus isolated from Indonesian regions share a highhomology with others ranging from 97-100%..Key words: sequencing, rabies, nucleoprotein gene (N), homology

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Phylogenetic analysis of nucleoprotein gene of dog rabies virus isolates from Southern India

Infection Genetics and Evolution ◽

10.1016/j.meegid.2009.04.004 ◽

2009 ◽

Vol 9 (5) ◽

pp. 976-982 ◽

Cited By ~ 19

Author(s):

T. Nagarajan ◽

S.B. Nagendrakumar ◽

B. Mohanasubramanian ◽

S. Rajalakshmi ◽

N.R. Hanumantha ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Rabies Virus ◽

Southern India ◽

Nucleoprotein Gene ◽

Virus Isolates

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Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line

Molecular Genetics Microbiology and Virology ◽

10.3103/s0891416820040060 ◽

2020 ◽

Vol 35 (4) ◽

pp. 237-242

Author(s):

Ya. M. Krasnov ◽

Zh. V. Alkhova ◽

S. V. Generalov ◽

I. V. Tuchkov ◽

E. A. Naryshkina ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Vero Cell ◽

Whole Genome Sequencing ◽

Cell Line ◽

Rabies Virus ◽

Genome Sequencing ◽

Virus Strain ◽

Whole Genome ◽

Vero Cell Line ◽

Rabies Virus Strain

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Phylogenetic analysis of glycoprotein (G) gene of rabies virus isolated from spotted deer, Delhi, 2016

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2018.04.4286 ◽

2018 ◽

Vol 73 ◽

pp. 385

Author(s):

G. Singh ◽

R. Jaiswal ◽

M. Chhabra ◽

N.K. Gupta ◽

M. Singhai ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Rabies Virus ◽

Glycoprotein G ◽

Spotted Deer

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Nucleotide Sequence and Phylogenetic Analysis of Glycoprotein-G of the Russian Fixed Rabies Virus Strain “Moscow 3253”

Problems of Particularly Dangerous Infections ◽

10.21055/0370-1069-2013-4-73-75 ◽

2013 ◽

pp. 73-75 ◽

Cited By ~ 1

Author(s):

I. V. Tuchkov ◽

Ya. M. Krasnov ◽

A. A. Goryaev ◽

Zh. V. Matveeva ◽

A. V. Stepanov ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Nucleotide Sequence ◽

Rabies Virus ◽

Virus Strain ◽

Glycoprotein G ◽

Rabies Virus Strain

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Molecular epidemiological analysis of wild animal rabies isolates from India

Veterinary World ◽

10.14202/vetworld.2019.352-357 ◽

2019 ◽

Vol 12 (3) ◽

pp. 352-357 ◽

Cited By ~ 2

Author(s):

Gundallhalli Bayyappa Manjunatha Reddy ◽

Rajendra Singh ◽

Karam Pal Singh ◽

Anil Kumar Sharma ◽

Sobharani Vineetha ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Fluorescent Antibody ◽

Antibody Test ◽

Reservoir Host ◽

Wild Animals ◽

Rt Pcr ◽

Epidemiological Analysis ◽

Nucleoprotein Gene ◽

Single Cluster

Aim: This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India. Materials and Methods: A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcription polymerase chain reaction (RT-PCR), and quantitative reverse transcriptase real-time PCR (RT-qPCR). The phylogenetic analysis of partial nucleoprotein gene sequences was performed. Results: Of 20 samples, 11, 10, and 12 cases were found positive by dFAT, RT-PCR, and RT-qPCR, respectively. Phylogenetic analysis showed that all Indian wild RVs isolates belonged to classical genotype 1 of Lyssavirus and were closely related to Arctic/Arctic-like single cluster indicating the possibility of a spillover of rabies among different species. Conclusion: The results indicated the circulation of similar RVs in sylvatic and urban cycles in India. However, understanding the role of wild animals as reservoir host needs to be studied in India.

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MOLECULAR-GENETIC CHARACTERIZATION OF FIELD ISOLATES OF RABIES VIRUS IDENTIFIED IN THE TERRITORY OF VLADIMIR, MOSCOW, TVER, NIZHNY NOVGOROD AND RYAZAN REGIONS

Voprosy Virusologii ◽

10.18821/0507-4088-2017-62-3-101-108 ◽

2017 ◽

Vol 62 (3) ◽

pp. 101-108

Author(s):

O. N. Zaykova ◽

T. V. Grebennikova ◽

A. M. Gulyukin ◽

A. A. Shabeykin ◽

I. V. Polyakova ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Rabies Virus ◽

Molecular Genetic ◽

Phylogenetic Group ◽

Molecular Genetic Study ◽

Field Isolates ◽

Vladimir Region ◽

Pcr Products ◽

Molecular Genetic Characterization

The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.

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