scholarly journals Determination of icariin content in dietary supplements by high performance liquid chromatography

2020 ◽  
Vol 3 (3) ◽  
pp. 145-153
Author(s):  
Thanh An Vu Thi ◽  
Dung Luong The ◽  
Thanh Hoa Mac Thi ◽  
Khanh Cao Cong ◽  
◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
Dietary Supplement ◽  
High Performance ◽  
Analytical Procedure ◽  
Ultrasonic Technique ◽  
Linear Calibration ◽  
Analytical Requirements

High performance liquid chromatography with PDA detector technique has been applied to analyze the Icariin content in dietary supplements that contains the ingredient of Epimedium. Sample preparation used methanol solvent and ultrasonic technique. Analytical procedure performed on HPLC Alliance-Waters e2695 system with chromatographic column C18 Reliant 2 (250 mm × 4.6 mm, 5 µm) and gradient between acetonitril and phosphoric acid 1% solution in 20 minutes. The validation results showed that the method had good specificity, linear calibration curve in the range of 0.42 - 42 µg/mL, the repeatability and recovery of the method meet the analytical requirements according to AOAC. The method has been deployed to analyze some dietary supplement products on the market and give Icariin content 6.15 - 54.3 mg/g (in dietary supplement) and 6.47 - 24.5 g/100g (in powder extracts).

scholarly journals HPLC-determination of active components in dietary supplement «L-CARNITINE smart»

2019 ◽  
pp. 86-96 ◽  
Author(s):  
O. D. Voitiuk ◽  
A. V. Yegorova ◽  
YU. V. Scrypynets ◽  
S. N. Kashutskуy ◽  
O. G. Kluchnik ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
Dietary Supplement ◽  
High Performance ◽  
Selective Separation ◽  
Active Components ◽  
Limit Of Quantitation

Dietary supplements are compositions of biologically active substances intended for consumption with food or addition to food products for the purpose of optimization of metabolic processes and functions of the human body. The dietary supplements include: vitamins, trace elements, amino acids, enzymes, proteins, probiotics, oils that can provide antioxidant, detoxifying, immunomodulatory, adaptogenic effects, etc. Detection of physiologically active components in dietary supplements is a difficult task and requires the use of modern highly informative research methods. One of the most powerful and versatile methods of determination is the method of high performance liquid chromatography (HPLC), combining the selective separation of the studied mixtures and high sensitivity. The purpose of this study was to develop a simple, rapid and selective method for determining ascorbic acid and L-carnitine L-tartrate in a multicomponent dietary supplement, produced in the form of sachets, using HPLC with spectrophotometric detection. The object of the study is the dietary supplement «L-CARNITINE smart», powder for oral solution of 16 g each in a sachet (INTERСHЕM). For the quantitative determination of the applied method of high-performance liquid chromatography. Chromatography was performed on an Agilent 1260 Infinity 2D LC System (USA) liquid chromatograph with a UV detector. The optimal conditions for the analysis have been experimentally determined: the type of sorbent, the composition of the eluent and its gradient, the wavelength and the detection time for ensuring the release of all components (including auxiliary substances) from the column, selective separation of ascorbic acid, potassium acesulfame and L-carnitine L-tartrate, minimization analysis time. The method has been validated according to the following parameters: specificity, linearity, accuracy, limit of quantitation.

Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

1994 ◽  
Vol 59 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Josef Královský ◽  
Marta Kalhousová ◽  
Petr Šlosar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Optimum Conditions ◽  
Linear Calibration ◽  
Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

scholarly journals Single Laboratory Validation of a Method for Determination of Glucosamine in RawMaterials and Dietary Supplements Containing Glucosamine Sulfate and/or Glucosamine Hydrochloride by High-Performance Liquid Chromatography with FMOC-Su Derivatization

2004 ◽  
Vol 87 (5) ◽  
pp. 1083-1092 ◽  
Author(s):  
Joseph ZiQi Zhou ◽  
Ted Waszkuc ◽  
Felicia Mohammed
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
High Performance ◽  
Raw Materials ◽  
Glucosamine Sulfate ◽  
Glucosamine Hydrochloride ◽  
Relative Standard ◽  
Single Laboratory

Abstract Single laboratory validation of a method for determination of glucosamine in raw materials and dietary supplements containing glucosamine sulfate and/or glucosamine hydrochloride by with high-performance liquid chromatography FMOC-Su derivatization. Tests with 2 blank matrixes containing SAMe, vitamin C, citric acid, chondroitin sulfates, methylsulfonylmethane, lemon juice concentrate, and other potential interferents showed the method to be selective and specific. Eight calibration curves prepared over 7 working days indicated excellent reproducibility with the linear range at least over 2.0–150 μg/mL, and determination coefficients >0.9999. Average spike recovery from the blank matrix (n = 8 over 2 days) was 93.5, 99.4, and 100.4% at respective spike levels of 15, 100, and 150%, and from the sample matrix containing glucosamine (n = 3) was 99.9 and 102.8% at respective levels of 10 and 40%, with relative standard deviations <0.9%. The method was also applied to 12 various glucosamine finished products and raw materials. The stability tests confirmed that glucosamine–FMOC-Su derivative once formed is stable at room temperature for at least 5 days. Limit of quantitation was 1 μg/mL and limit of detection was 0.3 μg/mL. The method is ready to proceed for the collaborative study.

scholarly journals Determination of Coenzyme Q10 in Over-the-Counter Dietary Supplements by High-Performance Liquid Chromatography with Coulometric Detection

2006 ◽  
Vol 89 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Peter H Tang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
Coenzyme Q10 ◽  
High Performance ◽  
Limit Of Detection ◽  
Detector Response ◽  
Over The Counter ◽  
Coulometric Detection

Abstract A rapid and sensitive method is described for the determination of coenzyme Q10 (Q10) in over-the-counter dietary supplements by automated high-performance liquid chromatography (HPLC) with coulometric detection. Sample solutions of powder-filled capsules, oil-based softgels, and tablets were prepared by serial dilution with 1-propanol. After dilution, a known volume of sample solution containing Q10 and the internal standard, coenzyme Q9 (Q9), was directly injected into the HPLC system. Most of electrochemically active compounds in the injection were oxidized at the precolumn conditioning cell and postcolumn guard cell. Q9 and Q10 were monitored at an analytical cell that contained 2 coulometric electrodes, where Q9 and Q10 were reduced to the corresponding ubiquinol-9 and -10 and then oxidized to produce currents. This method produced a linear detector response for peak height measurements over the concentration range of 0.058 g/mL (r > 0.999). The lower limit of detection was 5 ng/mL (signal-to-noise ratio, 3). The mean recovery was 98.9 0.6%; coefficients of variation for intra- and interday precisions were 1.84.0%. The proposed method was successfully applied to the determination of Q10 in marketed products.

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