The effect of deproteinization methods on the properties of glucosamine hydrochloride from shells of white leg shrimp (Litopenaeus vannamei) and black tiger shrimp (Penaeus monodon)

ABSTRACT: The effect of methods to remove protein content on the properties of glucosamine hydrochloride from the shells of white leg shrimp (Litopenaeus vannamei) and black tiger shrimp (Penaeus monodon) was investigated. Chitin from shrimp shells was obtained by demineralization in 6% HCl for 12h, deproteinization by two different methods (first group soaked in 8% NaOH for 36h and second group treated in Alcalase enzyme at the concentration of 0.2% for 36h). Two group samples were converted to glucosamine hydrochloride by soaking in 36.76% HCl solution for 5h at 85 °C. The results of fourier transform infrared spectroscopy (FTIR), solubility and recovery yield analysis showed that deproteinization methods did not significantly affect the properties of glucosamine hydrochloride. However, glucosamine hydrochloride from white leg shrimp shells contained higher recovery yield and solubility than black tiger shrimp shells.

Pemanfaatan Kitosan dari Cangkang Kepiting Bakau (Scylla serrata) dengan Metode Microwave sebagai Bahan Dasar Kapsul Obat

<p><span id="docs-internal-guid-d3b7bd3f-7fff-cc00-182f-e3199a97eb45"><span>Cangkang kepiting bakau (</span><span>Scylla serrata</span><span>) mengandung senyawa kitin yang dapat ditransformasi menjadi kitosan sebagai bahan pembuatan kapsul obat. Proses transfomasi ini masih perlu untuk dikembangkan lanjut. Penelitian ini bertujuan untuk menghasilkan kapsul obat berbahan dasar cangkang kepiting bakau dengan metode </span><span>microwave</span><span>. Metode isolasi kitin dari cangkang kepiting bakau meliputi proses deproteinasi, demineralisasi, dan dekolorisasi. Sintesis kitosan menggunakan metode </span><span>microwave </span><span>(daya 450 watt selama 15 menit) dalam pelarut NaOH 50% (b/v) dengan perbandingan 1:20 (b/v), selanjutnya kitosan dihidrolisis menggunakan larutan HCl 20% (v/v) untuk menghasilkan glukosamin hidroklorida (GlcN HCl). Pembuatan kapsul obat dengan perbandingan GlcN HCl dan larutan sukrosa yaitu masing-masing 3:1, 3:3, dan 3:5. Rendemen kitosan yang diperoleh sebanyak 37,5% dengan derajat deasetilasi 83,8%. Kapsul obat diperoleh perlakuan terbaik pada perbandingan GlcN HCl-larutan sukrosa 3:1. Berdasarkan analisis terhadap spektra kapsul obat, diidentifikasi adanya gugus O−H, −CH</span><span><span>3</span></span><span>, N−H, C−N, C−O, dan β-1,4-glikosidik. Karakteristik sifat fisik menunjukkan bahwa kapsul obat memiliki kadar air 12,7%, uji waktu hancur 13 menit 34 detik dan kelarutan dalam asam 3 menit 17 detik. Hasil penelitian menunjukkan bahwa kitosan cangkang kepiting bakau telah memenuhi kriteria bahan dasar kapsul obat sesuai kriteria farmakope Indonesia.</span></span></p><p><span><span><span id="docs-internal-guid-2e94a9c8-7fff-0821-25f9-df9a4ae907c6"><strong>Utilization of Chitosan from Mangrove Crab Shell (Scylla serrata</strong><span><strong>) using the Microwave Method as a Base Material for Medicinal Capsules</strong>. </span><span>The mangrove crab shell (</span><span>Scylla</span><span>serrata</span><span>) contains a chitin compound potentially transformed into chitosan as an ingredient for medicinal capsules. The research on this transformation process needs further developments. This research aims to produce chitin-based medicinal capsules of mangrove crab shells by microwave methods. The chitin isolation method of mangrove crab shells covers the process of deproteinization, demineralization, and decoloration. The synthesis of chitosan used microwave methods (450 watts of power for 15 minutes) in the solvent of 50% NaOH (w/v) with a ratio of 1:20 (b/v). Chitosan was then hydrolyzed using 20% HCl (v/v) solution to produce glucosamine hydrochloride (GlcN HCl). Preparation of drug capsules with a ratio of GlcN HCl and sucrose solution, namely 3:1, 3:3, and 3:5, respectively. The chitosan yield was obtained as much as 37.5% with a deacetylation degree of 83.8%. The best treatment of the medicinal capsules was obtained on the ratio of GlcN HCl and sucrose solution 3:1. The FTIR analysis of medicinal capsules are identified by the presence of the O−H, −CH</span><span><span>3</span></span><span>, N−H, C−N, C−O, and β-1.4-glycosidic. The physical characterization showed that the medicinal capsules have a water content of 12.7%, the test of destroyed time of 13 minutes 34 seconds, and soluble in acid that is 3 minutes 17 seconds. The results show that chitosan prepared from mangrove crab shell is potentially used as a basic ingredient for medicinal capsules because it met the criteria for Indonesian pharmacopoeial capsules.</span></span></span></span></p><p><span><span><br /></span></span></p>

USE OF SPECIAL PURPOSE PRODUCT FOR INCREASING PROTECTIVE FUNCTIONS OF THE ORGANISM OF SERVICEMEN WHEN PERFORMING TASKS IN EXTREME CONDITIONS

Extreme conditions are exceptional, special, extraordinary circumstances in which a civilian or military person often has to act, which requires them to show high skills, independence, activity, creativity, perseverance and determination in action, and require maximum stress, which goes sharply beyond limits of physiological norm. According to the Ministry of Health of Ukraine, cold injuries in the overall structure of injuries range from 3 to 10% and are accompanied by disability and sometimes fatal. Annually, more than 12,000 cases of cold injuries in our countryare registered. The problem is especially relevant given that fighting continues in the eastern Ukraine, in which the risk of cold injuries increases. Researchers at the National University of Pharmacy found that amino sugar glucosamine hydrochloride (G g / x) has frigoprotective properties, in particular: reduces the degree of body temperature, improves motor activity, muscle tone and physical activity, cardiohemodynamics and rheological properties of blood in the conditions of cold injuries. In addition, its frigoprotective properties in combination with gelatin are increased. Therefore, to create a special purpose product for increasing the protective functions of the body of servicemen when performing tasks in extreme conditions, the technology of marmalade was chosen, the recipe of which introduced amino sugar glucosamine hydrochloride in a fixed mass proportion. It is proved that the obtained product improves the body's adaptation to long-term effects of low temperatures and can be recommended as a frigoprotector when performing military service tasks in the field.

Comparison of Methods for Glucosamine Production from Achatina fulica Shells Waste

Osteoarthritis is a degenerative joint disease. This disease occurs when the joint feels painful due to mild inflammation that arises due to activity with the bone of end friction joints. Glucosamine has been proven to stimulate the production of cartilage and inhibit the enzyme that destroys cartilage. Glucosamine which was obtained from the hydrolysis of chitin occurs in a variety of animals such as the shell of crustacea, insects, arthropods, and the shell of molluscs (snail shells). There is a large snail population in Indonesia. Some restaurants and people make use by product snails as food sources. However, this process leaves snail shell as a by product. The high yield of glucosamine produced from snail shells use methods such as reflux+water bath and reflux+magnetic stirrer was 1.47%. Glucosamine hydrochloride was obtained through autoclave at 121 °C and pressure 1 atm for 70 minutes. The presence of chitin, chitosan, glucosamine was confirmed using FTIR spectroscopy. A joint pain ointment was prepared using glucosamine hydrochloride from snail shells concentration at 1 % w/w. Results of this research will be very useful for the reduction of agricultural pests while simultaneously generating a new product that can lift the economic value of snail shells. Keywords: Chitin, Chitosan, Glucosamine, Osteoarthritis, Snail Shells

Crystal structure of a salt with a protonated sugar cation and a cobalt(II) complex anion: (GlcN–H, K)[Co(NCS)4]·2H2O

The title compound, D-(+)-glucosammonium potassium tetrathiocyanatocobaltate(II) dihydrate, K(C6H14NO5)[Co(NCS)4]·2H2O or (GlcNH)(K)[Co(NCS)4]·2H2O, has been obtained as a side product of an incomplete salt metathesis reaction of D-(+)-glucosamine hydrochloride (GlcN·HCl) and K2[Co(NCS)4]. The asymmetric unit contains a D-(+)-glucosammonium cation, a potassium cation, a tetraisothiocyanatocobalt(II) complex anion and two water molecules. The water molecules coordinate to the potassium cation, which is further coordinated via three short K+...SCN− contacts involving three [Co(NCS)4]2− complex anions and via three O atoms of two D-(+)-glucosammonium cations, leading to an overall eightfold coordination around the potassium cation. Hydrogen-bonding interactions between the building blocks consolidate the three-dimensional arrangement.