A Bacterial Profile of Nosocomial Infections in a Tertiary Care Hospital

Background: Nosocomial infections, may appear either during the hospital stay of the patient or after discharge. Objective: To find out the bacterial profile of nosocomial infections in a tertiary care hospital. Methodology: This cross-sectional study was based on the records of the patients admitted in Ghurki Trust Teaching hospital during the period of January 2016 to December 2017, who developed infections after their hospital admission, and their record was available in the Microbiology section of the Department of Pathology. A total of 1000 complete records of the patients were retrieved. Bacterial culture tests from clinical samples of these patients were performed in the clinical microbiology Laboratory of Lahore Medical and Dental College. Specimens included in this study were urine samples, pus samples from wound discharge, infected implants, and dead necrotic tissue. Data were analyzed using SPSS version 26. Results: Out of 1000 samples, 150 (15%) samples showed positive growth, and among 150 83 (55%) were from females patients. The bacterial profile of these 150 positive samples showed that the most frequently isolated bacteria were Staphylococcus Aureus 45 (30%), MRSA 45 (30%) followed by Klebsiella, 21 (14%), Pseudomonas 15 (10%), E. Coli 12 (8%), Acinetobacter 9 (6%), and Proteus 3 (2%). Conclusion: Staph. Aureus, MRSA, Pseudomonas, Acinetobacter, Klebsiella, E.Coli and Proteus are frequently isolated bacteria from nosocomial infections in our study. Such studies should be done frequently to keep track of bacteria that are prevalent in hospital-acquired infections.