scholarly journals ALDH1 expression in inflammatory breast cancer tumor using Real-time RT-PCR gene expression quantifications: Moroccan prospective study

2021 ◽  
Vol 29 (2) ◽  
pp. 153-160
Author(s):  
Fouzia Mamouch ◽  
Abdelilah Laraqui ◽  
Narjiss Berrada ◽  
Hicham El Rhaffouli ◽  
Basma Elkhannousi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Prospective Study ◽  
Real Time ◽  
Inflammatory Breast Cancer ◽  
Rt Pcr ◽  
Cancer Tumor

scholarly journals BIF-1 Relative Gene Expression Study in Breast Cancer Tumor and Normal Adjacent Tissues using Real Time PCR

2017 ◽  
Vol 25 (3) ◽  
pp. 138-146
Author(s):  
Kazhaleh Mohammadi ◽  
Mahdiyeh Salimi ◽  
Abdolhamid Angaji ◽  
Foroozandeh Mahjoobi ◽  
Tayebeh Majidizadeh ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Relative Gene Expression ◽  
Gene Expression Study ◽  
Expression Study ◽  
Cancer Tumor ◽  
Relative Gene

scholarly journals BRCA1 Gene Expression in Breast Cancer: A Correlative Study between Real-time RT-PCR and Immunohistochemistry

2005 ◽  
Vol 53 (5) ◽  
pp. 621-629 ◽  
Author(s):  
Fahd Al-Mulla ◽  
Mahera Abdulrahman ◽  
Govindarajulu Varadharaj ◽  
Nadeem Akhter ◽  
Jehoram T. Anim
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Protein Expression ◽  
Real Time ◽  
Brca1 Gene ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Brca1 Protein ◽  
Brca1 Mrna ◽  
Cancer Tissues

Breast cancer is a major cause of cancer-related mortality in women. There are major discrepancies concerning the usefulness of various antibodies in detecting breast cancer susceptibility gene 1 (BRCA1) protein and its subcellular localization. The aim of the present study was to determine the specificity and sensitivity of immunohistochemistry (IHC) as a screening method for demonstrating BRCA1 expression. BRCA1 gene expression in archival paraffin-embedded breast cancer tissues was studied simultaneously at the protein and mRNA levels, and the two findings were compared. Forty-eight archival paraffin-embedded breast cancer tissues were studied for BRCA1 gene expression at protein level by IHC using four different antibodies against different BRCA1 epitopes and at mRNA level using real-time RT-PCR. BRCA1 mRNA expression was reduced or absent in 79% of the samples, and this finding correlated significantly with loss of BRCA1 protein expression in 83% of breast cancer tissues using one BRCA1 antibody studied (AB-1, against N-terminus epitope). The specificity of this antibody was 91.3%, and its sensitivity was 66.6%. There was no significant correlation between BRCA1 mRNA and protein expression as demonstrated by the remaining three antibodies. Antibody 8F7 had the highest sensitivity of 100%, but its specificity was 30.4% if mRNA levels were considered as the reference standard.

scholarly journals IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T

2006 ◽  
Vol 37 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Klaus W Frommer ◽  
Katharina Reichenmiller ◽  
Burkhardt S Schutt ◽  
Andreas Hoeflich ◽  
Michael B Ranke ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Growth Factor ◽  
Cell Line ◽  
Real Time ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Rt Pcr ◽  
Independent Effects

There is evidence that insulin-like growth factor-binding protein (IGFBP-2), a modulator of the actions of IGFs, also has IGF-independent effects in human tumor cell lines. These involve specific binding of IGFBP-2 to α5β1-integrin, followed by alterations in the phosphorylation status of downstream signaling molecules. Previously, IGFBP-2 has also been shown to be associated with cell proliferation, adhesion and migration. Here, we investigated direct effects of IGFBP-2 on apoptosis and alterations in the expression of related proteins. The breast cancer cell line Hs578T, which shows no IGFBP-2 production of its own and is independent of the IGF-I receptor, was treated with human recombinant IGFBP-2 in order to study the changes in gene expression induced by IGFBP-2. The methods employed for this purpose were oligonucleotide microarrays, real-time RT-PCR, western blotting, and immunoassays. Out of the 440 genes covered by the Oligo GEArray Human Cancer Microarray OHS-802, the expression of 77 genes was directly influenced by IGFBP-2. By the use of real-time quantitative RT-PCR, the gene expression of Nuclear Factor (NF)κB, p53, transforming growth factor β (TGF β-1), LAMB1 (Laminin, Beta 1), Bcl-2, and IIp45 was found to be significantly upregulated (by 1.2- to 3.05-fold; all P < 0.001). Accordingly, NFκB, p53, and TGF β-1 proteins, as measured by Western blotting and immunoassay, were upregulated > 1.5-fold. By using an ELISA-based and a flow cytometry-based apoptosis assay, IGFBP-2 was found to have a pro-apoptotic effect on Hs578T cells. Our results suggest that IGFBP-2-induced gene expressions are of functional significance for proliferation, cell adhesion, cell migration and apoptosis, and showed that IGFBP-2 can promote apoptosis in tumor cells independent of IGF.

scholarly journals TRIGONELLA FOENUM-GRAECUM L. EXHIBITS ESTROGENIC EFFECT THROUGH PRESENILIN 2 GENE EXPRESSION IN THE BREAST CANCER CELL LINE MCF-7

2019 ◽  
Vol 12 (1) ◽  
pp. 438
Author(s):  
Kurnia Agustini ◽  
Michael Wink ◽  
Wahono Sumaryono ◽  
Frans Suyatna ◽  
Nurjati Chairani Siregar
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cell Line ◽  
Active Fraction ◽  
Rt Pcr ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum ◽  
Presenilin 2 ◽  
Trigonella Foenum Graecum L ◽  
Mcf 7

Objective: The objective of this study is to investigate the estrogenic and antiestrogenic activity of Fenugreek seeds, Trigonella foenum-graecum L. in the estrogen-dependent breast cancer cell line, MCF-7, including its effect on the expression of estrogen-dependent presenilin 2 (pS2) gene.Methods: An activity guided fractionation was carried out with extracts from fenugreek seeds in MCF-7 cells. Cytotoxic activity assays were conducted with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Most fractions were also tested also tested in media with estradiol 10 nM We also analysed the expression of pS2 gene. For the analysis of pS2 gene expression we employed PCR primers for pS2 and for β-actin as a housekeeping gene using real-time polymerase chain reaction (RT-PCR).Results: Based on cytotoxic activity assay in MCF-7, the active fractions are ethyl acetic fraction and its phases ethyl acetic (EA) 2 and EA 2.2. The most active fraction was EA 2.2 (IC50=27.129 ppm), which exhibited a biphasic effect; at low concentrations, it stimulated the growth, and at high concentrations it showed strong cytotoxic effects. EA2.2 fraction in concentration 20 ppm, also could induce pS2 gene expression in media with and without estrogen.Conclusion: The most active fraction was the ethyl acetate phase and further subfractions. The most active fraction also induced the expression of pS2 gene which was studied by RT-PCR.

scholarly journals Quantitative hormone receptor (HR) expression and gene expression analysis in HR+ inflammatory breast cancer (IBC) vs non-IBC

2020 ◽  
Author(s):  
Toshiaki Iwase ◽  
Kenichi Harano ◽  
Hiroko Masuda ◽  
Kumiko Kida ◽  
Kenneth R. Hess ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Inflammatory Breast Cancer ◽  
Hormone Receptor ◽  
Survival Outcome ◽  
Stage Iii ◽  
Prognostic Role ◽  
Poor Survival ◽  
Poor Survival Outcome

Abstract Purpose: The purpose of this study was to determine the prognostic role of hormone receptor (HR) on inflammatory breast cancer (IBC) to elucidate its aggressive biological behavior.Methods: We evaluated the expression of estrogen receptor (ER) and progesterone receptor (PR) by immunohistochemical staining and determined the predictive and prognostic role of HR expression on 189 patients with HR+/HER2– IBC and 677 patients with HR+/HER2– stage III non-IBC. Furthermore, we performed gene expression (GE) analyses for 137 patients with HR+/HER2– IBC and 252 patients with corresponding non-IBC to detect genes that are specifically overexpressed in IBC.Results: The expression of ER% was significantly associated with longer distant disease-free survival and overall survival. However, there was no significant relationship between ER% and NAC outcome. In the GE study, 84 genes were identified as significantly distinguishing HR+ IBC from non-IBC. Among the top 15 canonical pathways expressed in IBC, the ERK/MAPK, PDGF, insulin receptor, and IL-7 signaling pathways were associated with the ER signaling pathway. Upregulation of the MYC gene was observed in three of these four pathways. Furthermore, HR+/HER2– IBC had significantly higher MYC amplification, and the genetic alteration was associated with poor survival outcome.Conclusions: Increased HR positivity was significantly associated with improved survival in both HR+/HER2– IBC and HR+/HER2– stage III non-IBC patients. HR+/HER2– IBC had several activated pathways with MYC upregulation, and the genetic alteration was associated with poor survival outcome. The results indicate that MYC may be a key gene for understanding the biology of HR+/HER2– IBC.

scholarly journals Detection of HER2 Status in Breast Cancer: Comparison of Current Methods with MLPA and Real-time RT-PCR

2013 ◽  
Vol 14 (12) ◽  
pp. 7621-7628 ◽  
Author(s):  
Reza Pazhoomand ◽  
Elahe Keyhan ◽  
Mehdi Banan ◽  
Hossein Najmabad ◽  
Masoud Karimlou ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Her2 Status ◽  
Rt Pcr
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