Dermoscopy in the Diagnosis of Scabies

ABSTRACT Scabies is one of the most common skin infestations and the diagnosis is mostly based on clinical presentation and suggestive history. A confirmatory diagnosis is highly desirable as the treatment requires strict compliance from the patient as well as close contacts. Existing diagnostic methods are time consuming and not very sensitive. This article describes the use of dermoscopy to facilitate the in-vivo diagnosis of this infestation. How to cite this article Grover C, Jakhar D. Dermoscopy in the Diagnosis of Scabies. Int J Dermoscop 2017;1(2):67-68.

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Chronic traumatic encephalopathy: clinical presentation and in vivo diagnosis

Sports Neurology - Handbook of Clinical Neurology ◽

10.1016/b978-0-444-63954-7.00027-6 ◽

2018 ◽

pp. 281-296 ◽

Cited By ~ 1

Author(s):

Steven D’Ascanio ◽

Michael L. Alosco ◽

Robert A. Stern

Keyword(s):

Clinical Presentation ◽

Chronic Traumatic Encephalopathy ◽

In Vivo Diagnosis

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Oral allergy syndrome by fruit and vegetable PR-10 allergy: Accuracy of in vivo diagnosis

Allergologia et Immunopathologia ◽

10.15586/aei.v49i2.28 ◽

2021 ◽

Vol 49 (2) ◽

pp. 129-132

Author(s):

Cristina De Rose ◽

Maria Letitzia Patti ◽

Alessadro Gambacorta ◽

Federica Brancato ◽

Stefano Miceli Sopo

Keyword(s):

Specific Ige ◽

Fruit And Vegetables ◽

Diagnostic Methods ◽

Oral Allergy Syndrome ◽

In Vitro Tests ◽

Prick Test ◽

Fresh Food ◽

In Vivo Diagnosis

Routine diagnostic methods for allergies to plant-derived foods are based on skin prick test (SPT) with commercial extracts, prick-by-prick (PbP) with fresh food, serum-specific IgE measurement, and oral food challenge.We discuss the possibility and the advantages of performing, in patients with oral allergy syn-drome (OAS) by fruit and vegetables (excluding nuts) PR-10 allergy, component-resolved diag-nosis (CRD) by SPT and PbP with raw and cooked vegetables, rather than performing a CRD with in vitro tests by drawing blood.Based on our clinical experience and the studies published in the literature, we believe that, at least for the OAS by fruit and vegetables (excluding nuts) PR-10 allergy, the search for sensitizing allergens and related cross-reactive allergens with SPT and PbP can be performed routinely in clinical practice, even at the primary-care level.

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Faculty Opinions recommendation of In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13139956.14470054 ◽

2011 ◽

Author(s):

Rienk Offringa

Keyword(s):

Pancreatic Cancer ◽

Molecular Imaging ◽

Early Stage ◽

Intraepithelial Neoplasia ◽

Pancreatic Intraepithelial Neoplasia ◽

In Vivo Diagnosis

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Aptamer Applications in Emerging Viral Diseases

Pharmaceuticals ◽

10.3390/ph14070622 ◽

2021 ◽

Vol 14 (7) ◽

pp. 622

Author(s):

Arne Krüger ◽

Ana Paula de Jesus Santos ◽

Vanessa de Sá ◽

Henning Ulrich ◽

Carsten Wrenger

Keyword(s):

Virus Assembly ◽

Viral Diseases ◽

Tissue Penetration ◽

Rna Molecules ◽

Viral Particles ◽

Pharmacokinetic Properties ◽

Oligonucleotide Library ◽

In Vivo Diagnosis ◽

Emerging Viral Diseases

Aptamers are single-stranded DNA or RNA molecules which are submitted to a process denominated SELEX. SELEX uses reiterative screening of a random oligonucleotide library to identify high-affinity binders to a chosen target, which may be a peptide, protein, or entire cells or viral particles. Aptamers can rival antibodies in target recognition, and benefit from their non-proteic nature, ease of modification, increased stability, and pharmacokinetic properties. This turns them into ideal candidates for diagnostic as well as therapeutic applications. Here, we review the recent accomplishments in the development of aptamers targeting emerging viral diseases, with emphasis on recent findings of aptamers binding to coronaviruses. We focus on aptamer development for diagnosis, including biosensors, in addition to aptamer modifications for stabilization in body fluids and tissue penetration. Such aptamers are aimed at in vivo diagnosis and treatment, such as quantification of viral load and blocking host cell invasion, virus assembly, or replication, respectively. Although there are currently no in vivo applications of aptamers in combating viral diseases, such strategies are promising for therapy development in the future.

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Where and Why Modeling Amyotrophic Lateral Sclerosis

International Journal of Molecular Sciences ◽

10.3390/ijms22083977 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3977

Author(s):

Francesco Liguori ◽

Susanna Amadio ◽

Cinzia Volonté

Keyword(s):

Amyotrophic Lateral Sclerosis ◽

Comparative Analysis ◽

Clinical Presentation ◽

In Vivo Models ◽

Comprehensive Review ◽

Common Features ◽

Neuroinflammatory Disease ◽

Multicellular Organisms ◽

Lateral Sclerosis

Over the years, researchers have leveraged a host of different in vivo models in order to dissect amyotrophic lateral sclerosis (ALS), a neurodegenerative/neuroinflammatory disease that is heterogeneous in its clinical presentation and is multigenic, multifactorial and non-cell autonomous. These models include both vertebrates and invertebrates such as yeast, worms, flies, zebrafish, mice, rats, guinea pigs, dogs and, more recently, non-human primates. Despite their obvious differences and peculiarities, only the concurrent and comparative analysis of these various systems will allow the untangling of the causes and mechanisms of ALS for finally obtaining new efficacious therapeutics. However, harnessing these powerful organisms poses numerous challenges. In this context, we present here an updated and comprehensive review of how eukaryotic unicellular and multicellular organisms that reproduce a few of the main clinical features of the disease have helped in ALS research to dissect the pathological pathways of the disease insurgence and progression. We describe common features as well as discrepancies among these models, highlighting new insights and emerging roles for experimental organisms in ALS.

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Lankesterella (Apicomplexa, Lankesterellidae) Blood Parasites of Passeriform Birds: Prevalence, Molecular and Morphological Characterization, with Notes on Sporozoite Persistence In Vivo and Development In Vitro

Animals ◽

10.3390/ani11051451 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1451

Author(s):

Carolina Romeiro Fernandes Chagas ◽

Josef Harl ◽

Vytautas Preikša ◽

Dovilė Bukauskaitė ◽

Mikas Ilgūnas ◽

...

Keyword(s):

Morphological Characterization ◽

Life Cycles ◽

Diagnostic Methods ◽

Host Cells ◽

Blood Parasites ◽

Serinus Canaria ◽

Long Time ◽

Development In Vitro

Recent studies confirmed that some Hepatozoon-like blood parasites (Apicomplexa) of birds are closely related to the amphibian parasite Lankesterella minima. Little is known about the biology of these pathogens in birds, including their distribution, life cycles, specificity, vectors, and molecular characterization. Using blood samples of 641 birds from 16 species, we (i) determined the prevalence and molecular diversity of Lankesterella parasites in naturally infected birds; (ii) investigated the development of Lankesterella kabeeni in laboratory-reared mosquitoes, Culex pipiens forma molestus and Aedes aegypti; and (iii) tested experimentally the susceptibility of domestic canaries, Serinus canaria, to this parasite. This study combined molecular and morphological diagnostic methods and determined 11% prevalence of Lankesterella parasites in Acrocephalidae birds; 16 Lankesterella lineages with a certain degree of host specificity and two new species (Lankesterella vacuolata n. sp. and Lankesterella macrovacuolata n. sp.) were found and characterized. Lankesterella kabeeni (formerly Hepatozoon kabeeni) was re-described. Serinus canaria were resistant after various experimental exposures. Lankesterella sporozoites rapidly escaped from host cells in vitro. Sporozoites persisted for a long time in infected mosquitoes (up to 42 days post exposure). Our study demonstrated a high diversity of Lankesterella parasites in birds, and showed that several avian Hepatozoon-like parasites, in fact, belong to Lankesterella genus.

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