Biological roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in solanaceous crops

WRKY proteins are a large superfamily of transcription factors that are involved in diverse biological processes including development, as well as biotic and abiotic stress responses in plants. WRKY family proteins have been extensively characterized and analyzed in many plant species, including Arabidopsis, rice, and poplar. However, knowledge on WRKY transcription factors in Santalum album is scarce. Based on S. album genome and transcriptome data, 64 SaWRKY genes were identified in this study. A phylogenetic analysis based on the structures of WRKY protein sequences divided these genes into three major groups (I, II, III) together with WRKY protein sequences from Arabidopsis. Tissue-specific expression patterns showed that 37 SaWRKY genes were expressed in at least one of five tissues (leaves, roots, heartwood, sapwood, or the transition zone), while the remaining four genes weakly expressed in all of these tissues. Analysis of the expression profiles of the 42 SaWRKY genes after callus was initiated by salicylic acid (SA) and methyl jasmonate (MeJA) revealed that 25 and 24 SaWRKY genes, respectively, were significantly induced. The function of SaWRKY1, which was significantly up-regulated by SA and MeJA, was analyzed. SaWRKY1 was localized in the nucleus and its overexpression improved salt tolerance in transgenic Arabidopsis. Our study provides important information to further identify the functions of SaWRKY genes and to understand the roles of SaWRKY family genes involved in the development and in SA- and MeJA-mediated stress responses.

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NAC transcription factors in plant abiotic stress responses

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms ◽

10.1016/j.bbagrm.2011.10.005 ◽

2012 ◽

Vol 1819 (2) ◽

pp. 97-103 ◽

Cited By ~ 468

Author(s):

Kazuo Nakashima ◽

Hironori Takasaki ◽

Junya Mizoi ◽

Kazuo Shinozaki ◽

Kazuko Yamaguchi-Shinozaki

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Stress Responses ◽

Nac Transcription Factors ◽

Plant Abiotic Stress

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GmNAC5, a NAC Transcription Factor, Is a Transient Response Regulator Induced by Abiotic Stress in Soybean

The Scientific World JOURNAL ◽

10.1155/2013/768972 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Hangxia Jin ◽

Guangli Xu ◽

Qingchang Meng ◽

Fang Huang ◽

Deyue Yu

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Stress Responses ◽

Apical Meristem ◽

High Salinity ◽

Response Regulator ◽

Mechanical Wounding ◽

Nac Transcription Factors ◽

Immature Seeds ◽

Hormone Signalling

GmNAC5 is a member of NAM subfamily belonging to NAC transcription factors in soybean (Glycine max(L.) Merr.). Studies on NAC transcription factors have shown that this family functioned in the regulation of shoot apical meristem (SAM), hormone signalling, and stress responses. In this study, we examined the expression levels ofGmNAC5.GmNAC5was highly expressed in the roots and immature seeds, especially strongly in immature seeds of 40 days after flowering. In addition, we found thatGmNAC5was induced by mechanical wounding, high salinity, and cold treatments but was not induced by abscisic acid (ABA). The subcellular localization assay suggested that GmNAC5 was targeted at nucleus. Together, it was suggested that GmNAC5 might be involved in seed development and abiotic stress responses in soybean.

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NAC transcription factors in plant multiple abiotic stress responses: progress and prospects

Frontiers in Plant Science ◽

10.3389/fpls.2015.00902 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 168

Author(s):

Hongbo Shao ◽

Hongyan Wang ◽

Xiaoli Tang

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Stress Responses ◽

Nac Transcription Factors

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Evolutionary Divergence and Biased Expression of NAC Transcription Factors in Hexaploid Bread Wheat (Triticum aestivum L.)

Plants ◽

10.3390/plants10020382 ◽

2021 ◽

Vol 10 (2) ◽

pp. 382

Author(s):

Jianhui Ma ◽

Meng Yuan ◽

Bo Sun ◽

Daijing Zhang ◽

Jie Zhang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Transcription Factors ◽

Abiotic Stress ◽

Hexaploid Wheat ◽

Evolutionary Divergence ◽

Snp Analysis ◽

Biotic And Abiotic Stress ◽

Nac Transcription Factors ◽

Group I ◽

Wide Range

The NAC genes, a large plant-specific family of transcription factors, regulate a wide range of pathways involved in development and response to biotic and abiotic stress. In this study, the NAC transcription factors were identified in 27 green plants, and the results showed that NAC transcription factors in plants undergo an appearance stage from water to land and a number expansion stage from gymnosperm to angiosperm. Investigating the evolutionary process of the NAC transcription factors from diploid species to hexaploid wheat revealed that tandem replications during the polyploidization process is an important event for increasing the number of NAC transcription factors in wheat. Then, the molecular characteristics, phylogenetic relationships, and expression patterns of 462 NAC transcription factors of hexaploid wheat (TaNACs) were analyzed. The protein structure results showed that TaNAC was relatively conservative at the N-terminal that contains five subdomains. All these TaNACs were divided into Group I and Group II by phylogenetic analysis, and the TaNACs in Group I should undergo strong artificial selection based on single nucleotide polymorphism (SNP) analysis. Through genome synteny and phylogenetic analysis, these TaNACs were classified into 88 groups and 9 clusters. The biased expression results of these TaNACs showed that there are 24 groups and 67 groups of neofunctionalization genes under biotic and abiotic stress, respectively, and 16 groups and 59 groups of subfunctionalization genes. This shows that neofunctionalization plays an important role in coping with different stresses. Our study provides new insights into the evolution of NAC transcription factors in hexaploid wheat.

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Genome-wide identification, phylogeny and expression analysis of AP2/ERF transcription factors family in sweet potato

BMC Genomics ◽

10.1186/s12864-021-08043-w ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Shutao He ◽

Xiaomeng Hao ◽

Shuli He ◽

Xiaoge Hao ◽

Xiaonan Chen

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Gene Family ◽

Sweet Potato ◽

Stress Responses ◽

Expression Patterns ◽

Gene Promoters ◽

Biotic And Abiotic Stress ◽

Protein Motifs ◽

Genome Wide

Abstract Background In recent years, much attention has been given to AP2/ERF transcription factors because they play indispensable roles in many biological processes, such as plant development and biotic and abiotic stress responses. Although AP2/ERFs have been thoroughly characterised in many plant species, the knowledge about this family in the sweet potato, which is a vital edible and medicinal crop, is still limited. In this study, a comprehensive genome-wide investigation was conducted to characterise the AP2/ERF gene family in the sweet potato. Results Here, 198 IbAP2/ERF transcription factors were obtained. Phylogenetic analysis classified the members of the IbAP2/ERF family into three groups, namely, ERF (172 members), AP2 (21 members) and RAV (5 members), which was consistent with the analysis of gene structure and conserved protein domains. The evolutionary characteristics of these IbAP2/ERF genes were systematically investigated by analysing chromosome location, conserved protein motifs and gene duplication events, indicating that the expansion of the IbAP2/ERF gene family may have been caused by tandem duplication. Furthermore, the analysis of cis-acting elements in IbAP2/ERF gene promoters implied that these genes may play crucial roles in plant growth, development and stress responses. Additionally, the available RNA-seq data and quantitative real-time PCR (qRT-PCR) were used to investigate the expression patterns of IbAP2/ERF genes during sweet potato root development as well as under multiple forms of abiotic stress, and we identified several developmental stage-specific and stress-responsive IbAP2/ERF genes. Furthermore, g59127 was differentially expressed under various stress conditions and was identified as a nuclear protein, which was in line with predicted subcellular localization results. Conclusions This study originally revealed the characteristics of the IbAP2/ERF superfamily and provides valuable resources for further evolutionary and functional investigations of IbAP2/ERF genes in the sweet potato.

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Genome-wide identification and expression analysis of NAC transcription factors in Ziziphus jujuba Mill. reveal their putative regulatory effects on tissue senescence and abiotic stress responses

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.114093 ◽

2021 ◽

Vol 173 ◽

pp. 114093

Author(s):

Meng Li ◽

Lu Hou ◽

Songshan Liu ◽

Chenxing Zhang ◽

Weicong Yang ◽

...

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Expression Analysis ◽

Stress Responses ◽

Nac Transcription Factors ◽

Genome Wide ◽

Ziziphus Jujuba ◽

Regulatory Effects

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Genome- wide characterization, expression profile analysis of WRKY family genes in Santalum album and functional identification of their role in abiotic stress

10.21203/rs.2.14060/v1 ◽

2019 ◽

Author(s):

Haifeng Yan ◽

Mingzhi Li ◽

Yuping Xiong ◽

Yueya Zhang ◽

Hanzhi Liang ◽

...

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Stress Responses ◽

Expression Profiles ◽

Expression Patterns ◽

Protein Sequences ◽

Santalum Album ◽

Biotic And Abiotic Stress ◽

Functional Identification ◽

Wrky Protein

Abstract Background: WRKY proteins are a large superfamily of transcription factors that are involved in diverse biological processes including development, as well as biotic and abiotic stress responses in plants. WRKY family proteins have been extensively characterized and analyzed in many plant species, including Arabidopsis , rice and poplar. However, knowledge on WRKY transcription factors in S antalum album is scarce. Results: Based on S . albu m genome and transcriptome data, 64 SaWRKY genes were identified in this study. A phylogenetic analysis based on the structures of WRKY protein sequences divided these genes into three major groups (I, II, III) together with WRKY protein sequences from Arabidopsis . Tissue-specific expression patterns showed that 37 SaWRKY genes were expressed in at least one of five tissues (leaves, roots, heartwood, sapwood, or the transition zone) while the remaining four genes were weakly expressed in all of these tissues. Analysis of the expression profiles of the 42 SaWRKY genes after callus was stimulated by salicylic acid (SA) and methyl jasmonate (MeJA) revealed that 34 and 19 SaWRKY genes, respectively were significantly induced. The function of SaWRKY1 , which was significantly up-regulated by SA and MeJA, was analyzed. SaWRKY1 was localized in the nucleus and its overexpression improved salt tolerance in transgenic Arabidopsis . Conclusions: Our study provides important information to further identify the functions of SaWRKY genes and to understand the roles of SaWRKY family genes involved in development and in SA- and MeJA-mediated stress responses.

Download Full-text