scholarly journals Compositional and functional properties of high-density lipoproteins in relation to coronary in-stent restenosis

2021 ◽  
Author(s):  
Shiva Shiva Ganjali ◽  
Elahe Mahdipour ◽  
Seyed Hamid Aghaee-Bakhtiari ◽  
Majid Ghayour-Mobarhan ◽  
Sara Saffar Soflaei ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Free Cholesterol ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
High Density ◽  
High Density Lipoproteins ◽  
Phospholipid Content ◽  
Cardiovascular Disease Risk Factor ◽  
Stent Restenosis ◽  
In Stent Restenosis

IntroductionIn-stent restenosis (ISR) is an unfavorable outcome that occurs in patients after coronary stenting. Using of drugs like statins as well as drug-eluting stents has only been partially effective in reducing the rate of ISR. Since low high-density lipoprotein cholesterol (HDL-C) concentration is a pivotal cardiovascular disease risk factor, this study aimed the evaluation of the compositional and functional alterations of HDL in individuals with ISR.Material and methodsThis case-control study comprised 21 ISR, 26 non-ISR, 16 angiography-negative, and 18 healthy subjects. Serum HDL2 (d: 1.063-1.125 g/mL) and HDL3 (d: 1.125-1.210 g/mL) subfractions were extracted from each subject using sequential ultracentrifugation. The capacity of HDL to efflux cellular cholesterol from lipid-loaded macrophages as well as to uptake free cholesterol (FC) from triglyceride-rich lipoproteins (TGRL) during lipolysis were assessed.ResultsNo difference was found in the HDL2 and HDL3 content of free cholesterol and total protein among the groups. NISR group showed reduced triglyceride content in HDL2 and increased phospholipid content in HDL3 relative to healthy subjects. Strong positive correlations were found between the cholesterol efflux capacity (CEC) of HDL2 and its phospholipid content in the healthy (r=0.50), angiography-negative (r=0.55) and ISR (r=0.52) groups. The capacity of apolipoprotein B (apoB)-depleted serum to uptake free cholesterol was not different among the groupsConclusionsDespite some compositional alterations, the capacity of HDL to efflux cholesterol from lipid-loaded macrophages as well as to uptake free cholesterol from TGRLs during lipolysis were not associated with ISR in this study

Abstract 631: Proteomic Characterization of Apolipoprotein A-II Defined Subfractions of Human High Density Lipoproteins

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Scott E Street ◽  
John T Melchior ◽  
Amy S Shah ◽  
W. S Davidson
Keyword(s):  
Physiological Function ◽  
Critical Role ◽  
Size Composition ◽  
High Density ◽  
Lipid Homeostasis ◽  
High Density Lipoproteins ◽  
Phospholipid Content ◽  
Hdl Function ◽  
Apolipoprotein A

High-density lipoproteins (HDL) play a critical role in lipid transport and vascular lipid homeostasis. HDL is heterogeneous, with particles varying in size, composition, and functionality. Proteomic studies have shown that HDL can host as many as 94 different proteins which can segregate into distinct subclasses. The two major apolipoproteins (apo)A-I and apoA-II account for 70% and 15-20%, respectively of HDL total protein. We hypothesized that the presence or absence of apoA-II can affect the binding of other proteins thus influencing HDL function. Immunoaffinity chromatography was used to isolate particles containing both apoA-I and apoA-II (LpA-I/LpA-II) and those with apoA-I with no apoA-II (LpA-I). These were isolated from both i ) total HDL isolated by ultracentrifugation (UC) and ii ) directly from plasma. The proteomic fingerprint of each population was determined by mass spectrometry. Within both populations, 54 total proteins were identified in UC isolated HDL compared to 98 proteins in particles isolated directly from plasma. In the UC isolated HDL, LpA-I/A-II particles contained 39 proteins compared to LpA-I particles which contained about 30. Interestingly, the opposite was true in particles isolated from plasma where upwards of 77 proteins on LpA-I/A-II particles were identified compared to LpA-I particles where 98 proteins were identified. Limited tryptic digestion experiments showed that LpA-I/A-II were more prone to proteolysis compared to LpA-I. Additionally, we found that LpA-I/A-II exhibited an increased capacity to efflux cholesterol from cultured macrophages (normalized by phospholipid content) suggesting a protein mediated difference in HDL functionality. Taken together, these results suggest the proteomic fingerprint of HDL subpopulations is heavily influenced by the method of isolation and that the proteomic pattern on HDL influences the physiological function of the particle.

Parental history and cardiovascular disease risk factor variables in children

1981 ◽  
Vol 10 (1) ◽  
pp. 25-37 ◽  
Author(s):  
Caroline V. Blonde ◽  
Larry S. Webber ◽  
Theda A. Foster ◽  
Gerald S. Berenson
Keyword(s):  
Cardiovascular Disease ◽  
Risk Factor ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Cardiovascular Disease Risk Factor ◽  
Parental History ◽  
Disease Risk Factor

scholarly journals Identification of Endothelial Proteins in Plasma Associated With Cardiovascular Risk Factors

2021 ◽  
Author(s):  
Maria J. Iglesias ◽  
Larissa D. Kruse ◽  
Laura Sanchez-Rivera ◽  
Linnea Enge ◽  
Philip Dusart ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cardiovascular Disease ◽  
Body Mass Index ◽  
Risk Factor ◽  
Total Cholesterol ◽  
Body Mass ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Cardiovascular Disease Risk Factor ◽  
Venous Disease

Objective: Endothelial cell (EC) dysfunction is a well-established response to cardiovascular disease risk factors, such as smoking and obesity. Risk factor exposure can modify EC signaling and behavior, leading to arterial and venous disease development. Here, we aimed to identify biomarker panels for the assessment of EC dysfunction, which could be useful for risk stratification or to monitor treatment response. Approach and Results: We used affinity proteomics to identify EC proteins circulating in plasma that were associated with cardiovascular disease risk factor exposure. Two hundred sixteen proteins, which we previously predicted to be EC-enriched across vascular beds, were measured in plasma samples (n=1005) from the population-based SCAPIS (Swedish Cardiopulmonary Bioimage Study) pilot. Thirty-eight of these proteins were associated with body mass index, total cholesterol, low-density lipoprotein, smoking, hypertension, or diabetes. Sex-specific analysis revealed that associations predominantly observed in female- or male-only samples were most frequently with the risk factors body mass index, or total cholesterol and smoking, respectively. We show a relationship between individual cardiovascular disease risk, calculated with the Framingham risk score, and the corresponding biomarker profiles. Conclusions: EC proteins in plasma could reflect vascular health status.

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