scholarly journals Influence of method of venous blood collection on the blood cell count determination result

2013 ◽  
Vol 3 ◽  
pp. 251-254
Author(s):  
Kinga Lis
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Venous Blood ◽  
Blood Collection ◽  
Blood Cell Count

scholarly journals Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Gas ◽  
Blood Cell Count ◽  
Animal Blood ◽  
Equus Asinus ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

scholarly journals Stability of Hematological Parameters in Woodland Caribou (Rangifer tarandus caribou) Blood Stored at 4°C

Rangifer ◽  
1996 ◽  
Vol 16 (4) ◽  
pp. 297
Author(s):  
G. Piat ◽  
S. Semalulu ◽  
Z. Florence ◽  
J. Nolan
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Rangifer Tarandus ◽  
Hematological Parameters ◽  
Blood Collection ◽  
Woodland Caribou ◽  
Blood Cell Count ◽  
Mean Cell Volume ◽  
Distribution Width ◽  
Complete Blood Counts

Eighteen free-ranging female woodland caribou were captured in northern Alberta in January and February 1993. Blood was collected into ethylenediaminetetraacetate (EDTA) tubes which were packaged in coolers containing ice packs, and transported to the laboratory where they arrived within 48 hrs of collection. Complete blood counts (CBC) were performed on five consecutive days to assess the stability of hematological parameters. Average values of hematocrit (HCT), mean cell hemoglobin (MCH), mean cell volume (MCV), red cell distribution width (P-J3W), white blood cell count (WBC), and red blood cell count (RJ3C) remained stable with no statistically significant changes occurring during 5 days of post-collection storage at 4°C. Mean PvBC values exhibited significant differences (p<0.05) between geographic locations. Mean platelet volume (MPV) increased significantly (p<0.001) with storage time, while platelet (PLT) values decreased (p<0.001) over time and were significantly different (p<0.01) between locations. For optimal hematological results, it is recommended that sample analysis be performed within 24 hours of blood collection; however, if caribou blood samples are properly stored at 4°C, useful information may be obtained from stable parameters up to 5 days following collection.

scholarly journals Erythrocyte alterations of the laying hens subjected to thermal stress

2021 ◽  
pp. 47911-47913
Keyword(s):  
Heat Stress ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Laying Hens ◽  
Control Group ◽  
Blood Collection ◽  
Blood Cell Count ◽  
Significant Difference ◽  
Red Blood Cell Count

The aim of this study was to identify erythrocyte changes resulting from heat stress in relation to different times of blood collection throughout the day. The experiment was carried out with 39 Rhode Island Red hens, in the initial laying phase, receiving standardized ad libitum feeding. The birds were placed in an environment of 20m2, with controlled temperature, divided into 2 distinct groups: Control Group with 12 animals at 16°C and Experimental Group with 27 animals at 30°C. Venipuncture took place at 7:00 am, 11:00 am and 3:00 pm. Blood was stored in an EDTA tube, aiming at cell preservation for further analysis. Total red blood cell count, hematimetry and erythroid morphometry were performed. After evaluating the erythroid and hematimetric parameters of the 39 birds, a negative correlation of heat stress was observed when comparing the different times of blood collection throughout the day. For morphometric correlation, there was a significant difference (P>0.01) at 3:00 pm. When evaluating the results between the times of blood collection from the same animal, it was observed that 55.5% reduced the hematocrit, while the total red blood cell count was reduced by 51.8%. It is essential that there is a systematization of time in the collection of blood from laying hens.

Cigarette Smoking Increases Thromboxane A2 Formation without Affecting Platelet Survival in Young Healthy Females

1992 ◽  
Vol 68 (05) ◽  
pp. 583-588 ◽  
Author(s):  
Annika Dotevall ◽  
Christina Rångemark ◽  
Elsa Eriksson ◽  
Jack Kutti ◽  
Hans Wadenvik ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cigarette Smoking ◽  
Blood Cell ◽  
Urinary Excretion ◽  
Life Span ◽  
Cell Count ◽  
Thromboxane A2 ◽  
Blood Cell Count ◽  
Platelet Activity ◽  
Pai 1

SummarySmoking is a risk factor for the development of atherosclerotic cardiovascular disease, in men as well as in women. An increased urinary excretion of the thromboxane metabolite 2,3-dinor-thromboxane B2 (Tx-M) has been observed in smokers of both genders, suggesting that cigarette smoking may facilitate cardiovascular disease via an action on the platelets. The present study addressed the hypothesis that the increased Tx-M excretion in female smokers reflects a true facilitation of platelet reactivity in vivo, rather than an increased destruction of the platelets. In healthy female volunteers (aged 20–46 years, 18 smokers and 17 non-smokers) platelet life-span and indices of platelet activity were determined, together with plasma levels of plasminogen activator inhibitor-1 (PAI-1), fibrinogen, peripheral blood cell counts and hematocrit. The urinary excretion of Tx-M was higher in smokers than in non-smokers (361 vs. 204 pg/mg creatinine, respectively, p <0.05), while plasma and urinary β-thromboglobulin, plasma platelet factor 4, platelet mean life-span and platelet production rate did not differ between the groups. PAI-1 activity, white blood cell count and hematocrit were higher in smokers than in non-smokers (p <0.05). These data indicate that smoking facilitates platelet formation of thromboxane A2 without affecting platelet survival; i.e. it increases the activity of platelets without affecting their viability to a measurable extent. Such an increase in platelet activity, operating in parallel to a reduced fibrinolytic activity and a higher hematocrit and white blood cell count, may play an etiological role in smoking-induced cardiovascular disease in women.

Influence of increased doses of fenbendazole supramolecular complex on sheep’s organism

2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Negative Influence ◽  
Supramolecular Complex ◽  
Control Group ◽  
Blood Cell Count ◽  
Russian Scientific ◽  
Russian Scientific Research Institute ◽  
Hematological And Biochemical Parameters

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.

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