In vitro antioxidant and anticancer activity of methanolic extract of Trachyspermum ammi seed on human oral cancer (KB) cell line

The present study was to determine the phytochemical constituents, antioxidant, and anticancer potential of methanolic extract from the seeds of Trachyspermumammi on KB, human oral cancer cell line using in the vitro assay system. The seeds of the T. Ammi were extracted with methanol. Phytochemical screening was done using standard qualitative biochemical tests. The antioxidant activity of the T. Ammi extract was determined by using a phosphomolybdenum assay.MTT assay was used to estimate the antiproliferative activity of the extract against the human oral cancer (KB) cell line. The apoptotic inducing ability of the extract was investigated by intracellular ROS analysis, antioxidant enzyme levels (SOD, CAT, GPx, and GSH), EtBr/AO staining, and TBARS analysis. The qualitative phytochemical analysis revealed the presence of different secondary metabolites. The phytochemical content present in the methanolic extract possessed high antioxidant capacity with transition metal reduction in phosphomolybdate assay. The methanol seed extract was found to be cytotoxic in vitro to (KB) oral cancer cell lines with IC50 value125µg/ml. Significantly, the T. Ammi seed extract showed a broad-spectrum anti-cancer activity through promoting ROS generation via modulating apoptosis induction in KBoral cancer cells. From the results, it can be concluded that T. ammiseed extract was a promising antioxidant and anticancer agent for KB human oral cancer cell line. The results figure the sufficient scientific backgrounds to isolate and purify the bioactive compounds in future for further applications.

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Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1745 ◽

2015 ◽

Vol 16 (9) ◽

pp. 709-714 ◽

Cited By ~ 6

Author(s):

Prachi Shivpuje ◽

Renuka Ammanangi ◽

Kishore Bhat ◽

Sandeep Katti

Keyword(s):

Oral Cancer ◽

Cytotoxic Activity ◽

Cell Line ◽

Cancer Cell ◽

Aqueous Extract ◽

Cancer Cell Line ◽

Ocimum Sanctum ◽

Oral Cancer Cell ◽

Oral Cancer Cell Line

ABSTRACT Background Cancer till today remains the leading cause of death in both developed and developing countries. Plants have been beacon of therapeutic sources for curing diseases from times immemorial. Hence, the present study aimed at evaluating the antiproliferative activity of extract of Ocimum sanctum leaves on oral cancer cell line. Objectives: • To evaluate the antiproliferative effect and to analyze dose dependent cytotoxic activity of aqueous extract of O. sanctum leaves on KB mouth cell line. • To compare the effectiveness among different variety of O. sanctum. Materials and methods KB cells (Mouth Epidermal Carcinoma Cells) were used for the present study. Aqueous and dry extract of O. sanctum with both dark (Krishna Tulsi) and light (Rama Tulsi) leaves were prepared in the institution. The antiproliferative and cytotoxic activity on KB cell line was evaluated by MTT assay. Statistical analysis with Mann-Whitney U-test and Wilcoxon matched pairs test was carried out. Results The aqueous extract of O. sanctum of both the leaves exhibited significant cytotoxic effect against oral cancer cell line. Conclusion Aqueous extract of O. sanctum leaves was effective as an antiproliferative agent which caused apoptosis in oral cancer cell line. Clinical significance Ocimum sanctum herb which is abundantly grown in India can be used for its anticancer properties for treating oral cancer. This will not only be cost-effective but will also have less or no side effects. How to cite this article Shivpuje P, Ammanangi R, Bhat K, Katti S. Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study. J Contemp Dent Pract 2015;16(9):709-714.

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