scholarly journals Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study

2013 ◽  
pp. 1-13 ◽  
Author(s):  
Masahiro Sato ◽  
Naoko Kubota ◽  
Emi Inada ◽  
Issei Saitoh ◽  
Masato Ohtsuka ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Hela Cells ◽  
Cell Types ◽  
Cytochemical Staining ◽  
Cell Study

scholarly journals The Effects of Morinda citrifolia (Noni) on the Cellular Viability and Osteogenesis of Stem Cell Spheroids

Medicina ◽  
2020 ◽  
Vol 56 (8) ◽  
pp. 389
Author(s):  
Sae Kyung Min ◽  
Jaekwen Oh ◽  
Jun-Beom Park
Keyword(s):  
Alkaline Phosphatase ◽  
Stem Cell ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Morinda Citrifolia ◽  
Herbal Remedies ◽  
Cellular Viability ◽  
Alizarin Red ◽  
Anthraquinone Dye ◽  
Cell Spheroids

Background and objectives: Morinda citrifolia (Noni) has been widely used in herbal remedies to treat and prevent various kinds of diseases. We conducted this study to evaluate the effects of Noni extract on the maintenance of morphology, the improvement of cellular viability, and the enhancement of osteogenesis of stem cell spheroids. Materials and Methods: We cultured stem cell spheroids made with gingiva-derived stem cells in the presence of Noni extract at concentrations of 10, 100 and 200 ng/mL. We performed analysis of the cell morphology and changes in the cellular viability. We conducted alkaline phosphatase activity assays using a kit, and mineralization assays using an anthraquinone dye to evaluate the osteogenesis of stem cell spheroids with the addition of Noni extract. Results: The applied cells formed spheroids well, and the addition of Noni at 10, 100 and 200 ng/mL concentrations did not produce significant morphological changes. The quantitative values for cellular viability on Day 3 showed that the absorbance values at 450 nm were 0.314 ± 0.013, 0.318 ± 0.008, 0.304 ± 0.000 and 0.300 ± 0.011 for Noni at 0, 10, 100 and 200 ng/mL concentrations, respectively. The results of alkaline phosphatase activity with absorbance values at 405 nm were 0.189 ± 0.019, 0.174 ± 0.023, 0.192 ± 0.014 and 0.210 ± 0.062 for Noni at 0, 10, 100 and 200 ng/mL concentrations, respectively, on Day 4. There were significantly higher values of Alizarin Red S staining for Noni in the 10, 100 and 200 ng/mL groups, with the highest value at 100 ng/mL when compared with the unloaded control on Day 14. Conclusions: Based on these findings, we concluded that Noni extract might be applied for the enhanced osteogenic differentiation of stem cell spheroids.

Induction of alkaline phosphatase activity in HeLa cells by sodium butyrate

In Vitro ◽  
1979 ◽  
Vol 15 (11) ◽  
pp. 861-864 ◽  
Author(s):  
Walker Wharton ◽  
Cathryn A. Hart ◽  
Barry Goz
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Sodium Butyrate ◽  
Hela Cells

scholarly journals ALKALINE PHOSPHATASE ACTIVITY IN HELA CELLS

1967 ◽  
Vol 15 (7) ◽  
pp. 417-418 ◽  
Author(s):  
J. HUGON ◽  
M. BORGERS ◽  
M. C. LONI
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Hela Cells

scholarly journals Alkaline Phosphatase Activity of Exudative Leukocytes in Acute Leukemia

Blood ◽  
1961 ◽  
Vol 18 (5) ◽  
pp. 572-580 ◽  
Author(s):  
PASQUALE E. PERILLIE ◽  
STUART C. FINCH
Keyword(s):  
Alkaline Phosphatase ◽  
Stem Cell ◽  
Acute Leukemia ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Acute Monocytic Leukemia ◽  
Monocytic Leukemia ◽  
Leukocyte Alkaline Phosphatase

Abstract A method for studying the leukocyte alkaline phosphatase activity in patients with acute leukemia is described. The technic overcomes the problem of obtaining sufficient numbers of mature granulocytes for study in such patients. Seven of 11 patients with acute monocytic leukemia were shown to have elevated levels of alkaline phosphatase activity. It is suggested that this method may be of assistance in classifying the stem cell forms of leukemia.

299 A FEATURE OF SELF-RENEWAL PORCINE EMBRYONIC STEM CELL-LIKE CELL LINES ESTABLISHED BY INHIBITORS

2013 ◽  
Vol 25 (1) ◽  
pp. 297
Author(s):  
S. Haraguchi ◽  
T. Tokunaga ◽  
T. Furusawa ◽  
K. Ohkoshi ◽  
M. Nakai ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Phosphatase Activity ◽  
Cell Lines ◽  
Alkaline Phosphatase Activity ◽  
Embryonic Stem ◽  
Self Renewal ◽  
Established Cell Lines ◽  
Established Cell

Despite meticulous attempts for more than two decades, establishment of authentic porcine embryonic stem cell (ESC) from pig has never been successful. Although putative porcine ESC-like cells have been reported, such cell lines easily lose the ability of self-renewal, becoming extinct or differentiating after only a limited number of passages in culture. Porcine ESC-like cells exhibiting the property of self-renewal rather than pluripotency are considered a valuable resource in applications such as drug screening and toxicology testing in humans and livestock, and in veterinary medicine. In the present study, we evaluated the effect of glycogen synthase kinase 3β (GSK3β) inhibitor CHIR99021 and Erk signalling inhibitor PD184352 for use in establishing ESC-like cell lines derived from the inner cell mass (ICM) of porcine blastocysts produced in vitro. These ICM-derived cell lines were initially cultured and passaged in conventional human ES medium. They displayed so-called ESC-like morphology; for example, the isolated colonies began to grow as a monolayer with coarse cell–cell boundaries, in which the cells exhibited polygonal boundaries, high nuclear/cytoplasmic ratios, abundant lipid-like inclusions, alkaline phosphatase activity, and expression of markers of undifferentiated cells such as OCT4 and NANOG. After transfer to culture in ES medium containing the inhibitors, the morphology of the colony was dramatically changed, displaying a closely packed and smooth-edged colony with tight cell–cell boundaries. Remarkably, growth of the established cell lines is leukemia inhibitory factor (LIF)-dependent. The inclusion of inhibitors supports self-renewal, thus enabling continuous culture for over 100 passages while maintaining an undifferentiated state. High-passage-number cells continued to express undifferentiated marker genes and showed alkaline phosphatase activity and telomerase activity with an X chromosome status of XaXi. We further investigated the potential for differentiation of the established cell lines. The cells could easily form embryoid body-like spheres in suspension culture. When either the spheres or ESC-like cells were inoculated under the kidney or testis capsules of nude mice, classical teratoma formation was not observed after 2 to 3 months. However, histological analyses revealed apparent invasive proliferation derived from porcine cells. Although further analyses are required to characterise the property of the porcine ESC-like cells, we have recently succeeded in establishment of green fluorescent protein (GFP)-expressing stable cells lines, which will be useful for further investigation.

scholarly journals A Study of the Effects of Doxorubicin-Containing Liposomes on Osteogenesis of 3D Stem Cell Spheroids Derived from Gingiva

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2693
Author(s):  
Hyunjin Lee ◽  
Jihwan Son ◽  
Sae Kyung Min ◽  
Chae-Bin Na ◽  
Gawon Yi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Stem Cell ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Cationic Liposomes ◽  
Alizarin Red S ◽  
Cellular Viability ◽  
Alizarin Red ◽  
Cell Spheroids ◽  
Calcium Deposits

The objective of the present investigation is to determine the effects of neutral, anionic, and cationic liposomes loaded with doxorubicin with thin-lipid-film-hydration method on the cellular viability and osteogenesis of stem cell spheroids. Spheroid formation and morphology of the three-dimensional spheroid were noted with an inverted microscope. Quantitative cellular viability was assessed using a commercially available kit. Osteogenic potential was evaluated by applying alkaline phosphatase activity and anthraquinone dye of Alizarin Red S. Western blot analysis was performed using collagen I expression. Spheroids were formed in each silicon elastomer-based concave microwell on Day 1. Noticeable changes of the spheroid were seen with a higher concentration of doxorubicin, especially in the cationic liposome group at Days 5 and 7. We found that the application of doxorubicin for 5 days significantly reduced the cellular viability. A higher concentration of doxorubicin produced a significant decrease in alkaline phosphatase activity. Alizarin Red S staining showed that extracellular calcium deposits were evenly noted in each group. An increase of calcium deposits was noted on Day 14 when compared to Day 7. The morphology of the groups with higher concentrations of doxorubicin showed to be more dispersed. We noticed that doxorubicin-loaded cationic liposomes resulted in the highest uptake of the examined cell spheroids and that doxorubicin-loaded liposomes affected the osteogenic differentiation. The implication of this study is that the type of liposome should be selected based on the purpose of the application.

scholarly journals Mouse ovarian alkaline phosphatase activities that respond to gonadotropins: histochemical and biochemical studies.

1976 ◽  
Vol 24 (10) ◽  
pp. 1101-1109 ◽  
Author(s):  
T A Bramley ◽  
J Kent
Keyword(s):  
Alkaline Phosphatase ◽  
Luteinizing Hormone ◽  
Phosphatase Activity ◽  
Chorionic Gonadotropin ◽  
Alkaline Phosphatase Activity ◽  
Human Chorionic Gonadotropin ◽  
Cell Types ◽  
Phosphatase Activities ◽  
Biochemical Studies ◽  
Different Cell Types

Alkaline phosphatase activity was measured in whole ovarian homogenates from pre-pubertal mice of different ages, with and without prior injection of human chorionic gonadotropin. Alkaline phosphatase activity was also scored in the different cell types in sections of similar ovaries, using two distinct histochemical procedures. The results from those methods differed. Biochemical studies indicated the presence of three distinct alakaline phosphatase activities: I and Ib, both optimal at pH 10.4 and with similar substrate requirements and inhibitor sensitivities (phosphatase I being characteristic of unstimulated ovaries and Ib of ovaries stimulated with human luteinizing hormone or human chorionic gonadotropin), and phosphatase II, optimal at pH 9.4, with different substrate requirements and inhibitor sensitivities. The differences observed using the histochemical procedures can probably be accounted for by the effects of different incubation conditions on the activities of these three enzymes.

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