scholarly journals Two new PCR-RFLPs in the domestic pigeon (<i>Columba livia</i> var. <i>domestica</i>) lactate dehydrogenase A (<i>LDH-A</i>) gene (Brief report)

2007 ◽  
Vol 50 (1) ◽  
pp. 112-113
Author(s):  
A. Dybus
Keyword(s):  
Lactate Dehydrogenase ◽  
Muscle Tissue ◽  
Spatial Orientation ◽  
Anaerobic Metabolism ◽  
Columba Livia ◽  
Domestic Pigeon ◽  
Homing Performance ◽  
Aerobic And Anaerobic ◽  
Ldha Gene ◽  
Selection Of

Abstract. Traditional selection of racing pigeons has been focusing on spatial orientation, velocity, and endurance of flight. LDHA gene is involved in aerobic and anaerobic metabolism of the muscle tissue (VAN HALL et al., 1999). Mutations in the LDHA gene can potentially diversify the homing performance of racing pigeons. Previously, two polymorphic sites of LDHA gene have been identified (DYBUS and KMIEĆ, 2002, DYBUS et al., 2006).

Lactate Dehydrogenase Inhibition: Biochemical Relevance and Therapeutical Potential

2019 ◽  
Vol 26 (18) ◽  
pp. 3242-3252 ◽  
Author(s):  
Giuseppina Laganá ◽  
Davide Barreca ◽  
Antonella Calderaro ◽  
Ersilia Bellocco
Keyword(s):  
Plasmodium Falciparum ◽  
Lactate Dehydrogenase ◽  
Anaerobic Metabolism ◽  
Catalytic Potential ◽  
Key Enzyme ◽  
Living Organisms ◽  
Almost All ◽  
Lactate Dehydrogenases ◽  
Ldh Isozymes ◽  
Selection Of

Lactate dehydrogenase (LHD) is a key enzyme of anaerobic metabolism in almost all living organisms and it is also a functional checkpoint for glucose restoration during gluconeogenesis and single-stranded DNA metabolism. This enzyme has a well preserved structure during evolution and among the species, with little, but sometimes very useful, changes in the amino acid sequence, which makes it an attractive target for the design and construction of functional molecules able to modulate its catalytic potential and expression. Research has focused mainly on the selection of modulator especially as far as LDH isozymes (especially LDH-5) and lactate dehydrogenases of Plasmodium falciparum (pfLDH) are concerned. This review summarizes the recent advances in the design and development of inhibitors, pointing out their specificity and therapeutic potentials.

scholarly journals Search for Human Lactate Dehydrogenase A Inhibitors Using Structure-Based Modeling

Acta Naturae ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 57-63 ◽  
Author(s):  
D. K. Nilov ◽  
E. A. Prokhorova ◽  
V. K. Švedas1
Keyword(s):  
Molecular Weight ◽  
Lactate Dehydrogenase ◽  
Virtual Screening ◽  
Anaerobic Metabolism ◽  
Low Molecular Weight ◽  
Closed Conformation ◽  
Potential Inhibitor ◽  
Low Molecular Weight Compounds ◽  
Potential Inhibitors ◽  
Selection Of

The human lactate dehydrogenase isoform A plays an important role in the anaerobic metabolism of tumour cells and therefore constitutes an attractive target in the oncology field. Full-atom models of lactate dehydrogenase A (in complex with NADH and in the apo form) have been generated to enable structure-based design of novel inhibitors competing with pyruvate and NADH. The structural criteria for the selection of potential inhibitors were established, and virtual screening of a library of low-molecular-weight compounds was performed. A potential inhibitor, STK381370, was identified whose docking pose was stabilized through additional interactions with the loop 96-111 providing for the transition from the open to the closed conformation.

Platelet Metabolism during the Interiorization of Two Different Types of Particulate Matter

1972 ◽  
Vol 27 (02) ◽  
pp. 263-271 ◽  
Author(s):  
I. A. Cooper ◽  
P Cochrane ◽  
B. G. Firkin ◽  
K. J. Pinkard
Keyword(s):  
Particulate Matter ◽  
Polymorphonuclear Leukocyte ◽  
Anaerobic Metabolism ◽  
Human Platelets ◽  
Metabolic Changes ◽  
Polystyrene Latex ◽  
Considerable Time ◽  
Difference Of Opinion ◽  
Different Types ◽  
Aerobic And Anaerobic

SummaryIt has been suggested that human platelets possess the ability to phagocytose particulate matter similar to the polymorphonuclear leukocyte. However some difference of opinion has arisen regarding this contention, particularly as differences have been demonstrated with regard to the observed metabolic changes occurring in platelets related to such a process.The experiments reported in this paper were designed to observe the aerobic and anaerobic metabolism in human platelets during and following interiorization of two different particles, viz. polystyrene latex and thorotrast. The results of these experiments show a marked difference between both types of particles with regard to observable metabolic changes despite the rapid interiorization of both types of material. Some alteration occurs in both aerobic and anaerobic metabolism a considerable time after interiorization of latex, whereas no alteration could be demonstrated after interiorization of thorotrast. It is suggested that the interiorization of particulate matter is by some process other than phagocytosis and that observed metabolic changes related to latex may be due to a release reaction.

scholarly journals The ldhA Gene Encoding Fermentative L-Lactate Dehydrogenase in Corynebacterium glutamicum Is Positively Regulated by the Global Regulator GlxR

2021 ◽  
Vol 9 (3) ◽  
pp. 550
Author(s):  
Koichi Toyoda ◽  
Masayuki Inui
Keyword(s):  
Lactate Dehydrogenase ◽  
Binding Site ◽  
Corynebacterium Glutamicum ◽  
Lactate Production ◽  
Exponential Phase ◽  
Aerobic Bacterium ◽  
Global Regulator ◽  
Gene Encoding ◽  
Fermentation Products ◽  
Ldha Gene

Bacterial metabolism shifts from aerobic respiration to fermentation at the transition from exponential to stationary growth phases in response to limited oxygen availability. Corynebacterium glutamicum, a Gram-positive, facultative aerobic bacterium used for industrial amino acid production, excretes L-lactate, acetate, and succinate as fermentation products. The ldhA gene encoding L-lactate dehydrogenase is solely responsible for L-lactate production. Its expression is repressed at the exponential phase and prominently induced at the transition phase. ldhA is transcriptionally repressed by the sugar-phosphate-responsive regulator SugR and L-lactate-responsive regulator LldR. Although ldhA expression is derepressed even at the exponential phase in the sugR and lldR double deletion mutant, a further increase in its expression is still observed at the stationary phase, implicating the action of additional transcription regulators. In this study, involvement of the cAMP receptor protein-type global regulator GlxR in the regulation of ldhA expression was investigated. The GlxR-binding site found in the ldhA promoter was modified to inhibit or enhance binding of GlxR. The ldhA promoter activity and expression of ldhA were altered in proportion to the binding affinity of GlxR. Similarly, L-lactate production was also affected by the binding site modification. Thus, GlxR was demonstrated to act as a transcriptional activator of ldhA.

A model-driven quantitative metabolomics analysis of aerobic and anaerobic metabolism inE. coliK-12 MG1655 that is biochemically and thermodynamically consistent

2013 ◽  
Vol 111 (4) ◽  
pp. 803-815 ◽  
Author(s):  
Douglas McCloskey ◽  
Jon A. Gangoiti ◽  
Zachary A. King ◽  
Robert K. Naviaux ◽  
Bruce A. Barshop ◽  
...  
Keyword(s):  
Anaerobic Metabolism ◽  
Model Driven ◽  
Quantitative Metabolomics ◽  
Aerobic And Anaerobic ◽  
Metabolomics Analysis

scholarly journals Aerobic and anaerobic metabolism for the zebrafish, Danio rerio, reared under normoxic and hypoxic conditions and exposed to acute hypoxia during development

2010 ◽  
Vol 70 (2) ◽  
pp. 425-434 ◽  
Author(s):  
WR Barrionuevo ◽  
MN Fernandes ◽  
O Rocha
Keyword(s):  
Danio Rerio ◽  
Anaerobic Metabolism ◽  
Developmental Stages ◽  
Acute Hypoxia ◽  
Lactate Concentration ◽  
Growth Delay ◽  
Hypoxic Conditions ◽  
Aerobic And Anaerobic ◽  
Mild Hypoxia

In order to verify the influence of chronic and acute ambient oxygen levels from egg to adult stage of the zebrafish, in vivo oxygen consumption (MO2), critical tensions of oxygen (Pcrit), heart rate (fH) and total body lactate concentration (Lc) were determined for Danio rerio (Hamilton, 1822) raised at 28 °C under normoxic (7.5 mgO2.L-1 or 80 mm.Hg-1) and hypoxic conditions (4.3 mgO2.L-1) and exposed to acute hypoxia during different developmental stages. Our findings confirmed that very early stages do not respond effectively to ambient acute hypoxia. However, after the stage corresponding to the age of 30 days, D. rerio was able to respond to acute hypoxia through effective physiological mechanisms involving aerobic and anaerobic metabolism. Such responses were more efficient for the fishes reared under hypoxia which showed that D. rerio survival capability increased during acclimation to mild hypoxia. Measurements of body mass and length showed that moderate hypoxia did not affect growth significantly until the fish reached the stage of 60 days. Moreover, a growth delay was verified for the hypoxic-reared animals. Also, the D. rerio eggs-to-larvae survival varied from 87.7 to 62.4% in animals reared under normoxia and mild hypoxia, respectively. However, the surviving animals raised under moderated hypoxia showed a better aptitude to regulate aerobic and anaerobic capacities when exposed to acute hypoxia.

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