Automated high resolution rain water sampler for stable water isotope monitoring

Author(s):  
Christoff Andermann ◽  
Torsten Queißer ◽  
Markus Reich ◽  
Bijaya Puri ◽  
Niels Hovius ◽  
...  

<p>With global climate change, one of the largest short-term threats to our societies comes from changes in the hydro-meteorological cycle: droughts, flooding and potentially increasing extreme rain events may have far greater direct impact on humans than rising temperatures alone. These changes often have sever consequences and widespread impact on society and ecosystems, yet they are difficult to track, trace and measure in order to fully understand the underlying process of delivering moisture and recharging water reservoirs. Only through the comprehensive monitoring of precipitation waters in space and time can we improve our process understanding and better predict the direction and magnitude of future hydro-meteorological changes, in particular on regional spatial scales. However, no commercial automated sampling solution exists, which fulfills the quality criteria for sophisticated hydrochemical water analysis. Here, we present an advanced prototype automatic precipitation water sampler for stable water isotope analysis of precipitation. The device is designed to be highly autonomous and robust for campaign deployment in harsh remote areas and fulfills the high demands on sampling and storage for isotope analysis (i.e. sealing of samples from atmospheric influences, no contamination and preservation of the sample material). The sampling device is portable, has low power consumption and a real-time adaptable sampling protocol strategy, and can be maintained at distance without any need to visit the location. Furthermore, the obtained water samples are not restricted to isotope analysis but can be used for any type of environmental water analysis. The current configuration can obtain 165 discrete rainwater samples with a minimum timely resolution of 5min or volume wise 2mm of rainfall. Our lab tests with dyed waters and waters with strongly differing isotopic signature demonstrate that the device can obtain, store and conserve samples without cross contamination over long periods of time. The device has been tested so far under several conditions, e.g. heavy summer thunderstorms with more than 50mm/24h of rainfall, sustained winter rainfall and in cold conditions involving melting of snow. This automated rainwater sampler provides an economic and sophisticated technological solution for monitoring moisture pathways and water transfer processes with the analytical quality of laboratory standard measurements on a new level of temporal and spatial resolution.</p>

2021 ◽  
Author(s):  
Christoff Andermann ◽  
Markus Reich ◽  
Torsten Queißer ◽  
Bijay Puri ◽  
Oliver Rach ◽  
...  

<p>With global change, one of the largest short-term threats to our societies comes from changes in the hydro-meteorological cycle: droughts, flooding and potentially increasing extreme rain events may have far greater direct impact on humans than rising temperatures alone. These changes often have sever consequences and widespread impact on society and ecosystems, yet they are difficult to track, trace and measure in order to fully understand the underlying process of delivering moisture and recharging water reservoirs. Only through the comprehensive monitoring of precipitation waters in space and time can we improve our process understanding and better predict the direction and magnitude of future hydro-meteorological changes, in particular on regional spatial scales. However, no commercial automated sampling solution exists, which fulfills the quality criteria for sophisticated hydrochemical water analysis.</p><p>Here, we present an new developed automatic precipitation water sampler for stable water isotope analysis of precipitation. The device is designed to be highly autonomous and robust for campaign deployment in harsh remote areas and fulfills the high demands on sampling and storage for isotope analysis (i.e. sealing of samples from atmospheric influences, no contamination and preservation of the sample material). The sampling device is portable, has low power consumption and a real-time adaptable sampling protocol strategy, and can be maintained at distance without any need to visit the location. Furthermore, the obtained water samples are not restricted to isotope analysis but can be used for any type of environmental water analysis. The current configuration can obtain 165 discrete rainwater samples with a minimum timely resolution of 5min or volume wise 2mm of rainfall.</p><p>The device was tested in several evaluation and benchmarking cycles. First lab tests with dyed waters and waters with strongly differing isotopic signature demonstrate that the device can obtain, store and conserve samples without cross contamination over long periods of time. The device has been tested so far under several conditions, e.g. heavy summer thunderstorms with more than 50mm/24h of rainfall, sustained winter rainfall and in cold conditions involving melting of snow. Furthermore, we run a benchmark test with several devices in parallel. Finally, in October 2020, we had installed six devices, in collaboration with Germany's National Meteorological Service (Deutscher Wetterdienst DWD), in a South-West to North-East transect across the Harz mountains in Germany. The transect covers ~ 100km distance along the main orographic gradient.</p><p>This automated rainwater sampler provides an economic and sophisticated technological solution for monitoring moisture pathways and water transfer processes with the analytical quality of laboratory standard measurements on a new level of temporal and spatial resolution.</p>


2019 ◽  
Vol 67 (1) ◽  
pp. 20-31 ◽  
Author(s):  
Andrea Rücker ◽  
Massimiliano Zappa ◽  
Stefan Boss ◽  
Jana von Freyberg

Abstract The contribution of snow meltwater to catchment streamflow can be quantified through hydrograph separation analyses for which stable water isotopes (18O, 2H) are used as environmental tracers. For this, the spatial and temporal variability of the isotopic composition of meltwater needs to be captured by the sampling method. This study compares an optimized snowmelt lysimeter system and an unheated precipitation collector with focus on their ability to capture snowmelt rates and the isotopic composition of snowmelt. The snowmelt lysimeter system consists of three individual unenclosed lysimeters at ground level with a surface of 0.14 m2 each. The unheated precipitation collector consists of a 30 cm-long, extended funnel with its orifice at 2.3 m above ground. Daily snowmelt samples were collected with both systems during two snowfall-snowmelt periods in 2016. The snowmelt lysimeter system provided more accurate measurements of natural melt rates and allowed for capturing the small-scale variability of snowmelt process at the plot scale, such as lateral meltwater flow from the surrounding snowpack. Because of the restricted volume of the extended funnel, daily melt rates from the unheated precipitation collector were up to 43% smaller compared to the snowmelt lysimeter system. Overall, both snowmelt collection methods captured the general temporal evolution of the isotopic signature in snowmelt.


2019 ◽  
Author(s):  
Tania Nasreen ◽  
Nora A. S. Hussain ◽  
Mohammad Tarequl Islam ◽  
Fabini D. Orata ◽  
Paul C. Kirchberger ◽  
...  

ABSTRACTVibrio metoecus is a recently described and little studied causative agent of opportunistic infections in humans, often coexisting with V. cholerae in aquatic environments. However, the relative abundance of V. metoecus with V. cholerae and their population dynamics in aquatic reservoirs is still unknown. We developed a multiplex qPCR assay with a limit of detection of three copies per reaction to simultaneously quantify total V. metoecus and V. cholerae abundance, as well as the toxigenic and O1 serogroup subpopulations of V. cholerae from environmental samples. Four different genes were targeted as specific markers for individual Vibrio species or subpopulations; viuB, a gene encoding a vibriobactin utilization protein, was used to quantify the total V. cholerae population. The cholera toxin gene ctxA provided an estimation of toxigenic V. cholerae abundance, while the rfbO1 gene specifically detected and quantified V. cholerae belonging to the O1 serogroup, which includes almost all lineages of the species responsible for the majority of past and ongoing cholera pandemics. To measure V. metoecus abundance, the gene mcp, encoding methyl accepting chemotaxis protein, was used. Marker specificity was confirmed by testing several isolates of V. cholerae and V. metoecus alongside negative controls of isolates within and outside of the Vibrio genus. Analysis of environmental water samples collected from four different geographic locations including cholera-endemic (Dhaka, Kuakata and Mathbaria in Bangladesh) and non-endemic (Oyster Pond in Falmouth, Massachusetts, USA) regions showed that V. metoecus was only present in the USA site, recurring seasonally. Within the coastal USA site, the non-toxigenic O1 serogroup represented up to ∼18% of the total V. cholerae population. V. cholerae toxigenic O1 serogroup was absent or present in low abundance in coastal Bangladesh (Kuakata and Mathbaria) but constituted a relatively high proportion of the total V. cholerae population sustained throughout the year in inland Bangladesh (Dhaka). A preference for host/particle attachment was observed, as the majority of cells from both Vibrio species (>90%) were identified in the largest water size fraction sampled, composed of particles or organisms >63 μm and their attached bacteria. This is the first study to apply a culture-independent method to quantify V. cholerae or V. metoecus directly in environmental reservoirs of areas endemic and non-endemic for cholera on significant temporal and spatial scales.SIGNIFICANCECholera is a life-threatening disease that requires immediate intervention; it is of prime importance to have fast, accurate and sensitive means to detect V. cholerae. Consistent environmental monitoring of the abundance of V. cholerae along with its toxigenic and O1 serogroup subpopulations could facilitate the determination of the actual distribution of this organism in aquatic reservoirs and thus help to predict an outbreak before it strikes. The lack of substantial temporal and spatial environmental sampling, along with specific quantitative measures, has made this goal elusive so far. The same is true for V. metoecus, a close relative of V. cholerae which has been associated with several clinical infections and could likely pose an emerging threat, readily exchanging genetic material with its more famous relative.


2016 ◽  
Vol 20 (8) ◽  
pp. 3167-3182 ◽  
Author(s):  
Jian Peng ◽  
Alexander Loew ◽  
Xuelong Chen ◽  
Yaoming Ma ◽  
Zhongbo Su

Abstract. The Tibetan Plateau (TP) plays a major role in regional and global climate. The understanding of latent heat (LE) flux can help to better describe the complex mechanisms and interactions between land and atmosphere. Despite its importance, accurate estimation of evapotranspiration (ET) over the TP remains challenging. Satellite observations allow for ET estimation at high temporal and spatial scales. The purpose of this paper is to provide a detailed cross-comparison of existing ET products over the TP. Six available ET products based on different approaches are included for comparison. Results show that all products capture the seasonal variability well with minimum ET in the winter and maximum ET in the summer. Regarding the spatial pattern, the High resOlution Land Atmosphere surface Parameters from Space (HOLAPS) ET demonstrator dataset is very similar to the LandFlux-EVAL dataset (a benchmark ET product from the Global Energy and Water Cycle Experiment), with decreasing ET from the south-east to north-west over the TP. Further comparison against the LandFlux-EVAL over different sub-regions that are decided by different intervals of normalised difference vegetation index (NDVI), precipitation, and elevation reveals that HOLAPS agrees best with LandFlux-EVAL having the highest correlation coefficient (R) and the lowest root mean square difference (RMSD). These results indicate the potential for the application of the HOLAPS demonstrator dataset in understanding the land–atmosphere–biosphere interactions over the TP. In order to provide more accurate ET over the TP, model calibration, high accuracy forcing dataset, appropriate in situ measurements as well as other hydrological data such as runoff measurements are still needed.


1991 ◽  
Vol 7 (Supple) ◽  
pp. 733-736
Author(s):  
TAKASHI KORENAGA ◽  
YOSHIYUKI YONO

2009 ◽  
Vol 99 (4) ◽  
pp. 347-358 ◽  
Author(s):  
J.A. Bennett ◽  
D.R. Gillespie ◽  
S.L. VanLaerhoven

AbstractOmnivory involves numerous feeding relationships and a complex web of interactions. When using omnivores in biocontrol, these interactions need to be understood to maximize feeding on the target species and minimize non-target interactions. Dicyphus hesperus is used along with Encarsia formosa for biocontrol of whiteflies in greenhouse tomato crops. Dicyphus hesperus is a generalist omnivore which feeds on all components of the system. To quantify these interactions, stable isotope analysis was used to identify trophic position with nitrogen isotopes (δ15N) and plant sources with carbon isotopes (δ13C). Feeding trials were used to establish baseline isotopic data for D. hesperus and their diet, including Verbascum thapsus, an alternative plant food. Cage trials were used to monitor population abundances and the isotopic signature of D. hesperus. In feeding trials, D. hesperus were enriched relative to their food, suggesting an elevated trophic position. However, large amounts of isotopic variation were found within all diet components, with only V. thapsus exhibiting a distinct signature. In cage trials, the average δ15N and δ13C of the omnivore declined over time, coinciding with declines in total available prey, though it may be confounded by changes in temperature. The range of δ13C, but not the range of δ15N, also declined over time. This suggests a change in the plant source within the diet, but also some unquantified variability within the population. We suggest that diet variability exists within D. hesperus populations, declining as prey become less abundant.


2019 ◽  
Vol 70 (9) ◽  
pp. 1295 ◽  
Author(s):  
Ivor Stuart ◽  
Clayton Sharpe ◽  
Kathryn Stanislawski ◽  
Anna Parker ◽  
Martin Mallen-Cooper

Worldwide, riverine fish are the target of environmental water because populations have declined in lotic river habitats following river regulation. Murray cod is an endangered Australian riverine fish with remaining populations associated with lotic river reaches with instream habitat, including some creeks operated as part of irrigation systems. Our objectives were to develop a life history model, apply the building block method of environmental flows to enhance the abundance of juvenile Murray cod and promote population recovery. From 2008 to 2018 we evaluated changes to Murray cod juvenile abundance before and after implementation of a perennial environmental flow regime that began in 2013. During the first year of the environmental flow, larvae were collected as evidence of spawning. Murray cod abundance can be enhanced with environmental flows that target: (1) an annual spring spawning or recruitment flow with no rapid water level drops; (2) maximising hydrodynamic complexity (i.e. flowing habitats that are longitudinally continuous and hydrodynamically complex); and (3) an annual base winter connection flow. Recognition that incorporating hydraulics (water level and velocity) at fine and coarse time scales, over spatial scales that reflect life histories, provides broader opportunities to expand the scope of environmental flows to help restore imperilled fish species in regulated ecosystems.


2000 ◽  
Vol 57 (11) ◽  
pp. 2280-2292 ◽  
Author(s):  
Brian P Kennedy ◽  
Joel D Blum ◽  
Carol L Folt ◽  
Keith H Nislow

To distinguish Atlantic salmon (Salmo salar) populations in tributaries of the Connecticut River, we studied the incorporation and stability of Sr isotopes in juvenile salmon. We established the geologic basis for unique isotopic signatures in 29 salmon sites. Stream-specific Sr isotopic ratios (87Sr/86Sr) were found in calcified tissues of salmon parr within 3 months of stocking. We found little seasonal variation in the Sr signatures of stream water or fish tissue. There were no significant differences among the Sr signatures of otoliths, scales, and vertebrae. For mature salmon raised under constant conditions, 70% of the Sr isotopic signature in calcified tissues was derived from food sources. We developed a criterion for identifying moving fish based upon the isotopic variability of genetically marked fish. Applying this criterion to our streams, 7% of the fish in our study had incorporated Sr from multiple streams. Strontium isotopes distinguished all 8 regions in the White River basin and 7 of the 10 regions in the West River basin. When watersheds are considered together, Sr isotopes differentiated 11 unique signatures from 18 regions. We conclude that Sr isotopes are an effective marking tool and discuss ways in which they can be combined with other marking techniques over larger spatial scales.


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