scholarly journals Design and deployment of autoclave pressure vessels for the portable deep-sea drill rig MeBo (<i>Meeresboden-Bohrgerät</i>)

2017 ◽  
Vol 23 ◽  
pp. 29-37 ◽  
Author(s):  
Thomas Pape ◽  
Hans-Jürgen Hohnberg ◽  
David Wunsch ◽  
Erik Anders ◽  
Tim Freudenthal ◽  
...  

Abstract. Pressure barrels for sampling and preservation of submarine sediments under in situ pressure with the robotic sea-floor drill rig MeBo (Meeresboden-Bohrgerät) housed at the MARUM (Bremen, Germany) were developed. Deployments of the so-called MDP (MeBo pressure vessel) during two offshore expeditions off New Zealand and off Spitsbergen, Norway, resulted in the recovery of sediment cores with pressure stages equaling in situ hydrostatic pressure. While initially designed for the quantification of gas and gas-hydrate contents in submarine sediments, the MDP also allows for analysis of the sediments under in situ pressure with methods typically applied by researchers from other scientific fields (geotechnics, sedimentology, microbiology, etc.). Here we report on the design and operational procedure of the MDP and demonstrate full functionality by presenting the first results from pressure-core degassing and molecular gas analysis.

Geobiology ◽  
2004 ◽  
Vol 2 (4) ◽  
pp. 217-223 ◽  
Author(s):  
L. MAUCLAIRE ◽  
K. ZEPP ◽  
P. MEISTER ◽  
J. MCKENZIE

2021 ◽  
Author(s):  
◽  
Joseph Graham Prebble

<p>The response of the surface ocean and terrestrial climate in the New Zealand region to interglacial Marine Isotope Stage (MIS) 11 (423-380ka) is documented, using assemblages of fossilised marine algae (dinoflagellate cysts, or dinocysts) and spores/pollen from terrestrial plants, analysed from marine sediment cores. This work is underpinned by studies on the modern distribution of dinocysts, factors that influence their accumulation in marine sediment, and the use of dinocyst assemblages to quantify past sea surface temperature (SST). In the first of the modern-process studies, a dataset of modern sea-floor dinocyst assemblages from the Southern Hemisphere is collated, including new observations from the SW Pacific. Variations in the assemblages are related to environmental gradients. Cluster analysis reveals distinct biogeographic assemblage zones, individual taxa indicative of specific water masses are identified, while ordination of the databases indicates that the assemblages vary most with changes in SST. A second modern process study reports on the dinocyst assemblages from two time-incremental sediment traps (3 years of data) moored north and south of the Subtropical Front in the ocean east of New Zealand. This study provides observations of seasonal and inter-annual variability of dinocyst flux to the deep sea, which are used to identify possible biases in the sea-floor dinocyst assemblages. Observations from these first two studies are used in a systematic analysis of the strengths and weakness of using dinocyst assemblages to quantify SST in the SW Pacific. The best transfer function performance achieved was a root mean squared error of 1.47˚C, for an artificial neural network model, and the benefits in considering a range of model results are also established. Fossil records that document the oceanographic and terrestrial response to MIS11 are developed from two areas around New Zealand; (i) dinocysts assemblages are collected from the east Tasman Sea, from giant piston cores MD06-2987, -2988, and 2989, and (ii) dinocysts and pollen assemblages are analysed from Deep Sea Drilling Project (DSDP) Site 594, from the east of New Zealand. Dinocyst assemblages confirm that SST in the east Tasman Sea was ~2-3˚C warmer than the present during late MIS11 (415-400ka), while SSTs were slightly below modern levels during an early phase (428-415ka). Two assemblage – based productivity indices suggest that the elevated SSTs during MIS11 were accompanied by lower rates of primary productivity in the east Tasman Sea study area than the present. As in the east Tasman Sea, two distinct phases of MIS11 are recognised in both the dinocyst and pollen assemblages at DSDP 594. The dinocyst assemblages of late MIS11 are similar to, but qualitatively represent warmer waters than the Holocene. The succession of pollen assemblages during MIS12-11 is very similar to that observed during the previous two interglacials at this site (MIS1 and MIS5), with two notable variations: (i) the deglacial vegetation succession during MIS11 was prolonged, and (ii) the pollen assemblage representing the warmest forest type was also present for longer (ca. 15ky) than later interglacials. Changes in the pollen record during MIS11 at DSDP 594 correlate more closely to SST variations in the east Tasman Sea than to ocean variations at DSDP 594, suggesting that the eastern ocean had only limited influence on conditions on the adjacent landmass during MIS11.</p>


1976 ◽  
Vol 22 (11) ◽  
pp. 1667-1671 ◽  
Author(s):  
A. F. Carlucci ◽  
Susan L. Shimp ◽  
Peter A. Jumars ◽  
Hans W. Paerl

Deep-sea and sediment bacteria at the bottom of an approximately 1200-m water column were sampled by means of pressure vessels attached to a remote underwater manipulator. Cells were immediately fixed in situ with glutaraldehyde, and after processing in the laboratory their morphologies were observed with the scanning electron microscope. Most bacteria were coccoid or rod-lide and less than 0.4 μm in diameter or width. Few filamentous bacteria were observed. Bacteria were in aggregates or free-living. It is concluded that morphologies of deep-sea bacteria collected and fixed at the hydrostatic pressure of their environment are, in general, similar to the observed morphologies of deep-sea bacteria determined at 1 atm pressure after collection and decompression during ascent through the water column.


2018 ◽  
Vol 27 (0) ◽  
pp. 87-97 ◽  
Author(s):  
Tadashi Maruyama ◽  
Tomo-o Watsuji ◽  
Tomoko Takahashi ◽  
Hiromi Kayama Watanabe ◽  
Yukiko Nagai ◽  
...  
Keyword(s):  
Deep Sea ◽  

2013 ◽  
Vol 10 (8) ◽  
pp. 5639-5649 ◽  
Author(s):  
D. de Beer ◽  
M. Haeckel ◽  
J. Neumann ◽  
G. Wegener ◽  
F. Inagaki ◽  
...  

Abstract. This study focused on biogeochemical processes and microbial activity in sediments of a natural deep-sea CO2 seepage area (Yonaguni Knoll IV hydrothermal system, Japan). The aim was to assess the influence of the geochemical conditions occurring in highly acidic and CO2 saturated sediments on sulfate reduction (SR) and anaerobic methane oxidation (AOM). Porewater chemistry was investigated from retrieved sediment cores and in situ by microsensor profiling. The sites sampled around a sediment-hosted hydrothermal CO2 vent were very heterogeneous in porewater chemistry, indicating a complex leakage pattern. Near the vents, droplets of liquid CO2 were observed emanating from the sediments, and the pH reached approximately 4.5 in a sediment depth > 6 cm, as determined in situ by microsensors. Methane and sulfate co-occurred in most sediment samples from the vicinity of the vents down to a depth of 3 m. However, SR and AOM were restricted to the upper 7–15 cm below seafloor, although neither temperature, low pH, nor the availability of methane and sulfate could be limiting microbial activity. We argue that the extremely high subsurface concentrations of dissolved CO2 (1000–1700 mM), which disrupt the cellular pH homeostasis, and lead to end-product inhibition. This limits life to the surface sediment horizons above the liquid CO2 phase, where less extreme conditions prevail. Our results may have to be taken into consideration in assessing the consequences of deep-sea CO2 sequestration on benthic element cycling and on the local ecosystem state.


2017 ◽  
Vol 34 (5) ◽  
pp. 983-1000 ◽  
Author(s):  
Keith Jackson ◽  
Ursula Witte ◽  
Stewart Chalmers ◽  
Erik Anders ◽  
John Parkes

AbstractThe investigation of benthic biodiversity and biogeochemical processes in the deep sea is complicated by the need to conduct experiments at in situ pressures. Recovery of sediment samples to the surface without maintaining full-depth ambient pressure may damage the organisms that are of interest or cause physiological changes that could influence the processes being studied. It is possible to carry out in situ experiments using remotely operated vehicles (ROVs) or lander systems. However, the costs and complexity of ROV operations are significant and, for both ROVs and landers, the complexity and repeatability of the experiments are subject to the limitations imposed by these platforms. A system is described—the Multi-Autoclave Corer Experiment (MAC-EXP)—that has been developed with the aim of offering a new experimental approach to investigators. The MAC-EXP system is designed to retrieve sediment cores from depths down to 3500 m and to seal them into pressure chambers before being recovered so that they are maintained at their normal ambient pressure. After recovery the core chambers can be connected to a laboratory incubation system that allows for experimentation on the sediment without loss of pressure and under controlled conditions of temperature and oxygen concentration. The system is relatively low cost when compared to ROV systems and can be deployed using methods and equipment similar to those used for routine deployments of small unpressurized multicorers. The results of sea trials are detailed.


2013 ◽  
Vol 10 (2) ◽  
pp. 1899-1927 ◽  
Author(s):  
D. de Beer ◽  
M. Haeckel ◽  
J. Neumann ◽  
G. Wegener ◽  
F. Inagaki ◽  
...  

Abstract. This study focused on biogeochemical processes and microbial activity in sediments of a natural deep-sea CO2 seepage area (Yonaguni Knoll IV hydrothermal system, Japan). The aim was to assess the influence of the geochemical conditions occurring in highly acidic and CO2 saturated sediments on sulphate reduction (SR) and anaerobic methane oxidation (AOM). Porewater chemistry was investigated from retrieved sediment cores and in situ by microsensor profiling. The sites sampled around a sediment-hosted hydrothermal CO2 vent were very heterogeneous in porewater chemistry, indicating a complex leakage pattern. Near the vents, droplets of liquid CO2 were observed to emanate from the sediments, and the pH reached approximately 4.5 in a sediment depth >6 cm, as determined in situ by microsensors. Methane and sulphate co-occurred in most sediment samples from the vicinity of the vents down to a depth of at least 3 m. However, SR and AOM were restricted to the upper 7–15 cm below seafloor, although neither temperature, low pH, nor the availability of methane and sulphate could be limiting microbial activity. We argue that the extremely high subsurface concentrations of dissolved CO2 (1000–1700 mM), through the ensuing high H2CO3 levels (approx. 1–2 mM) uncouples the proton-motive-force (PMF) and thus inhibits biological energy conservation by ATPase-driven phosphorylation. This limits life to the surface sediment horizons above the liquid CO2 phase, where less extreme conditions prevail. Our results may have to be taken into consideration in assessing the consequences of deep-sea CO2 sequestration on benthic element cycling and on the local ecosystem state.


2017 ◽  
Author(s):  
Eriko Shimada ◽  
Yusuke Tsuruwaka

We recently found Cribrinopsis japonica Tsutsui & Tsuruwaka, 2014 (Shinkai-hakutou-ginchaku in Japanese name) at the depth between 384 and 800 m in Toyama Bay, Sea of Japan. Since then, C. japonica has been reared under atmospheric pressure in the laboratory for seven years. C. japonica may use a fluorescent protein carried in its tentacles to lure shrimp (Tsutsui et al., 2016*1). However, the ecology of C. japonica in the deep-sea is hardly known. To elucidate the unknown ecology, we coupled one of the first long-term in situ studies of deep-sea organisms with complementary laboratory experiments. Our exploration of deep-sea benthos revealed that C. japonica inhabits the deepest areas of the sea floor at 1,960 m. Moreover, 80% of C. japonica in the deep-sea stayed together with the deep-sea shrimp. In the laboratory environment, when we added the same shrimp species which was observed in situ to the rearing tank with C. japonica, C. japonica stayed closer with the shrimp without attacking using the tentacles. It is rare to observe different animals together at one place or space since there are very few animals in the ocean floor at > 1,000 m depth in the Sea of Japan (Motokawa & Kajihara, 2017*2). In such depopulated environment, it is conceivable that C. japonica and the shrimp may receive benefit mutually or one side by establishing a ‘symbiotic relationship.’ We will elucidate their relationship in more details by studying the possible ‘symbiosis’ in the laboratory.


Nature ◽  
1986 ◽  
Vol 323 (6085) ◽  
pp. 251-253 ◽  
Author(s):  
Craig R. Smith ◽  
Peter A. Jumars ◽  
David J. DeMaster

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