Age and AgNor- A Morphometric study

To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA ) values in buccal epithelial cells of healthy individuals in different age groups. Material and Methodology:- In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups.Group1-10-20year, Group2-20- 30year, Group3-30-40year, Group4-40-50year, Group5-50-60year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Results: Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. Conclusion: There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.

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Biomonitoring of oral epithelial cells in smokers and non-smokers submitted to panoramic X-ray: comparison between buccal mucosa and lateral border of the tongue

Clinical Oral Investigations ◽

10.1007/s00784-009-0345-6 ◽

2009 ◽

Vol 14 (6) ◽

pp. 669-674 ◽

Cited By ~ 19

Author(s):

Fernanda Angelieri ◽

Tatiana de Cássia Gonçalves Moleirinho ◽

Viviane Carlin ◽

Celina Tizuko Fujiyama Oshima ◽

Daniel Araki Ribeiro

Keyword(s):

Epithelial Cells ◽

Buccal Mucosa ◽

Lateral Border ◽

X Ray ◽

Oral Epithelial Cells ◽

Oral Epithelial

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Quantitative detection of Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa in human oral epithelial cells from subjects with periodontitis and periodontal health

Journal of Medical Microbiology ◽

10.1099/jmm.0.055830-0 ◽

2013 ◽

Vol 62 (10) ◽

pp. 1592-1600 ◽

Cited By ~ 29

Author(s):

Andrea V. Colombo ◽

Graziela M. Barbosa ◽

Daniela Higashi ◽

Giorgio di Micheli ◽

Paulo H. Rodrigues ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Epithelial Cells ◽

Periodontal Disease ◽

Quantitative Detection ◽

Healthy Individuals ◽

Quantitative Real Time Pcr ◽

Periodontal Health ◽

Oral Epithelial Cells ◽

Oral Epithelial

Epithelial cells in oral cavities can be considered reservoirs for a variety of bacterial species. A polymicrobial intracellular flora associated with periodontal disease has been demonstrated in buccal cells. Important aetiological agents of systemic and nosocomial infections have been detected in the microbiota of subgingival biofilm, especially in individuals with periodontal disease. However, non-oral pathogens internalized in oral epithelial cells and their relationship with periodontal status are poorly understood. The purpose of this study was to detect opportunistic species within buccal and gingival crevice epithelial cells collected from subjects with periodontitis or individuals with good periodontal health, and to associate their prevalence with periodontal clinical status. Quantitative detection of total bacteria and Staphylococcus aureus, Pseudomonas aeruginosa and Enterococcus faecalis in oral epithelial cells was determined by quantitative real-time PCR using universal and species-specific primer sets. Intracellular bacteria were visualized by confocal microscopy and fluorescence in situ hybridization. Overall, 33 % of cell samples from patients with periodontitis contained at least one opportunistic species, compared with 15 % of samples from healthy individuals. E. faecalis was the most prevalent species found in oral epithelial cells (detected in 20.6 % of patients with periodontitis, P = 0.03 versus healthy individuals) and was detected only in cells from patients with periodontitis. Quantitative real-time PCR showed that high levels of P. aeruginosa and S. aureus were present in both the periodontitis and healthy groups. However, the proportion of these species was significantly higher in epithelial cells of subjects with periodontitis compared with healthy individuals (P = 0.016 for P. aeruginosa and P = 0.047 for S. aureus). Although E. faecalis and P. aeruginosa were detected in 57 % and 50 % of patients, respectively, with probing depth and clinical attachment level ≥6 mm, no correlation was found with age, sex, bleeding on probing or the presence of supragingival biofilm. The prevalence of these pathogens in epithelial cells is correlated with the state of periodontal disease.

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Biomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue

Environment International ◽

10.1016/j.envint.2009.06.001 ◽

2009 ◽

Vol 35 (7) ◽

pp. 1062-1065 ◽

Cited By ~ 19

Author(s):

Renato A. Martins ◽

Guilherme A. da Silva Gomes ◽

Odair Aguiar ◽

Daniel A. Ribeiro

Keyword(s):

Epithelial Cells ◽

Buccal Mucosa ◽

Lateral Border ◽

Petrol Station ◽

Oral Epithelial Cells ◽

Oral Epithelial

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Evaluation of Occupational Exposure to Xylene in Petroleum Workers by Assessing Urinary Methyl Hippuric Acid and Cellular changes of Exfoliated Epithelial Cells in Buccal Mucosa Smears

International Journal of Recent Technology and Engineering - 2 ◽

10.35940/ijrte.b1946.078219 ◽

2019 ◽

Vol 8 (2) ◽

pp. 1323-1328

Keyword(s):

Occupational Exposure ◽

Epithelial Cells ◽

Buccal Mucosa ◽

Acid Level ◽

Hippuric Acid ◽

Work Shift ◽

Oral Epithelial Cells ◽

Cellular Changes ◽

Oral Epithelial

Xylene is an important component of petrol and a widely distributed environmental contaminant. About 98% of Xylene is derived from the petrochemical and petroleum refining industries. When human get expose to xylene, which is one of the major Geno toxicants, may be associated with a range of acute/chronic diseases and cancer still literature is not available. Taking into our mind that occupational exposure to such derivatives may possess genotoxic risk or not. Hence our study aims to investigate and correlate the cellular changes in exfoliated oral epithelial cells oral smears with urinary methyl hippuric acid level estimation in petrol pump workers and to identify the significant role of xylene on oral mucosa. Materials and Methods: Urine samples and oral buccal mucosa smears were collected from 30 healthy individual (control) and 30 petroleum pump workers (case) working in petroleum station who are above 18 years of age. The urine was collected before exposure/work shift and after completion of work shift. The urinary methyl hippuric acid (MHA) level was analyzed by using Shimadzu UV-Visible Spectrophotometer procedure. The smeared slides were stained with PAP stain and analyses the cytomorphometric changes of exfoliated epithelial cells by using Axio Vision SE64 Rel 4.9.1. Ink Software. Results: The urinary Methyl hippuric acid level was substantially higher in cases than in controls (p<0.001). The Micronuclei (MN) frequency was drastically increased in cases than in controls and was statistically highly significant (P<0.0001). The frequency of MN gradually increased along with increased urinary MHA level in petroleum pump workers (case).

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Faculty Opinions recommendation of From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.11552956.12602054 ◽

2011 ◽

Author(s):

Judith Berman ◽

Anja Forche

Keyword(s):

Candida Albicans ◽

Epithelial Cells ◽

Oral Epithelial Cells ◽

Oral Epithelial

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Assessment of the Effect of Complete Denture Adhesives on Oral Epithelial Cells’ DNA in Elderly Patients with Controlled Diabetes

Egyptian Dental Journal ◽

10.21608/edj.2018.77272 ◽

2018 ◽

Vol 64 (3) ◽

pp. 2747-2756

Author(s):

Noha El-Shaheed ◽

Amira Gomaa ◽

Ashraf Zakaria

Keyword(s):

Epithelial Cells ◽

Elderly Patients ◽

Complete Denture ◽

Oral Epithelial Cells ◽

Denture Adhesives ◽

Oral Epithelial

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Novel Aggregation Properties of Candida albicans Secreted Aspartyl Proteinase Sap6 Mediate Virulence in Oral Candidiasis

Infection and Immunity ◽

10.1128/iai.00282-15 ◽

2015 ◽

Vol 83 (7) ◽

pp. 2614-2626 ◽

Cited By ~ 35

Author(s):

Rohitashw Kumar ◽

Darpan Saraswat ◽

Swetha Tati ◽

Mira Edgerton

Keyword(s):

Candida Albicans ◽

Epithelial Cells ◽

Oral Candidiasis ◽

Oral Microbiome ◽

Proteinase Activity ◽

Adhesion Properties ◽

Content Type ◽

Oral Epithelial Cells ◽

Oral Epithelial ◽

Cell Cell

Candida albicans, a commensal fungus of the oral microbiome, causes oral candidiasis in humans with localized or systemic immune deficiencies. Secreted aspartic proteinases (Saps) are a family of 10 related proteases and are virulence factors due to their proteolytic activity, as well as their roles in adherence and colonization of host tissues. We found that mice infected sublingually withC. albicanscells overexpressing Sap6 (SAP6OE and a Δsap8strain) had thicker fungal plaques and more severe oral infection, while infection with the Δsap6strain was attenuated. These hypervirulent strains had highly aggregative colony structurein vitroand higher secreted proteinase activity; however, the levels of proteinase activity ofC. albicansSaps did not uniformly match their abilities to damage cultured oral epithelial cells (SCC-15 cells). Hyphal induction in cells overexpressing Sap6 (SAP6OE and Δsap8cells) resulted in formation of large cell-cell aggregates. These aggregates could be produced in germinated wild-type cells by addition of native or heat-inactivated Sap6. Sap6 bound only to germinated cells and increasedC. albicansadhesion to oral epithelial cells. The adhesion properties of Sap6 were lost upon deletion of its integrin-binding motif (RGD) and could be inhibited by addition of RGD peptide or anti-integrin antibodies. Thus, Sap6 (but not Sap5) has an alternative novel function in cell-cell aggregation, independent of its proteinase activity, to promote infection and virulence in oral candidiasis.

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