scholarly journals A Comparison of a Modified Formalin-Ether Concentration Technique with a Commercial (Feconomics®) Method and Its Two Modifications in the Investigation of Blastocystis sp.

2018 ◽  
Author(s):  
Merve Aydın ◽  
İlkiz Oğuz ◽  
Katren Al-bakkour ◽  
Meryem Çolak ◽  
Funda Doğruman Al ◽  
...  
Keyword(s):  
Concentration Technique ◽  
Blastocystis Sp

Enterovirus Concentration Using Automated Hollow Fiber Ultrafiltration

1982 ◽  
Vol 14 (4-5) ◽  
pp. 257-272 ◽  
Author(s):  
G Belfort ◽  
A Paluszek ◽  
L S Sturman
Keyword(s):  
Drinking Water ◽  
Hollow Fiber ◽  
Enhanced Recovery ◽  
Tap Water ◽  
Membrane Surface ◽  
Creeping Flow ◽  
Hollow Fibers ◽  
Virus Concentration ◽  
Concentration Technique ◽  
Solution Interface

The Automated Hollow Fiber Ultrafiltration (AHFU) method is proposed here as a simple, efficient and rapid virus concentration technique from tap and drinking water sources. The results reported here extend the testing of the AHFU method to include two Picornaviruses [Poliovirus 2 (vaccine) and Echovirus 1] and Reovirus 3. Their respective mean virus recoveries from between 3 and 100 l of tap water is 88 ± 26, 79 ± 60, and 104 ± 48%. Various approaches including membrane surface modification, changes in backwash hydrodynamics, modification of the feed and backwash composition, and the use of S35-methionine labelled Poliovirus 2, are used to study the recovery of sorbed Poliovirus 2 from the hollow fiber/solution interface. An increase in the backwash pH to between 9.5 and 10.5 significantly improved Poliovirus 2 recovery. This, together with the labelled experiments, indicates that the virus-membrane interactions are probably electrostatic in nature. Convective polarization during filtration probably brings the virus close enough to the surface for these interactions to occur since virus losses were not detected for a non-permeation recycle experiment. Because very low Reynold's numbers are used, the flow is in the creeping-flow-regime for both filtration and backwashing (axial and radial). Unless significantly higher Reynolds could be used, enhanced recovery due to purely hydrodynamic forces is unlikely. High Reynold's numbers, of course, are limited by the pressure constraints of the hollow fibers.

scholarly journals Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established?

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 340
Author(s):  
Fernando Salvador ◽  
Beatriz Lobo ◽  
Lidia Goterris ◽  
Carmen Alonso-Cotoner ◽  
Javier Santos ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Microscopic Examination ◽  
Parasitic Infection ◽  
Diagnostic Technique ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference ◽  
Blastocystis Sp ◽  
Irritable Bowel ◽  
Asymptomatic Subjects

Background: The aim of the present study is to describe the occurrence of Blastocystis sp. detection among asymptomatic subjects and patients with irritable bowel syndrome in order to evaluate the potential association between irritable bowel syndrome and the parasitic infection. Methods: Cross-sectional study where adult patients with irritable bowel syndrome diagnosed according to Rome IV criteria were included. A control group was formed by asymptomatic subjects older than 18 years. Exclusion criteria were: immunosuppressive condition or having received any drug with demonstrated activity against Blastocystis sp. within the last 6 months before study inclusion. Epidemiological and clinical information was collected from all included participants. Two stool samples were obtained from all participants: one sample for microscopic examination and one sample for Blastocystis sp. PCR detection. Blastocystis sp. infection was defined by the positivity of any of the diagnostic techniques. Results: Seventy-two participants were included (36 asymptomatic subjects and 36 patients with irritable bowel syndrome). Thirty-five (48.6%) were men, and median age of participants was 34 (IQR 29–49) years. The overall rate of Blastocystis sp. carriage was 27.8% (20/72). The prevalence assessed through microscopic examination was 22.2% (16/72), while the prevalence measured by PCR was 15.3% (11/72). When comparing the presence of Blastocystis sp. between asymptomatic subjects and IBS patients, we did not find any statistically significant difference (36.1% vs. 19.4% respectively, p = 0.114). Conclusions: regarding the occurrence of Blastocystis sp., no differences were found between asymptomatic participants and patients with irritable bowel disease irrespective of the diagnostic technique performed.

Molecular identification and subtyping of Blastocystis sp. in hospital patients in Central China

2021 ◽  
pp. 125796
Author(s):  
Junqiang Li ◽  
Haiju Dong ◽  
Md Robiul Karim ◽  
Xianli Yang ◽  
Liqin Chao ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Central China ◽  
Hospital Patients ◽  
Blastocystis Sp

scholarly journals Molecular Subtyping of Blastocystis sp. Isolated from Farmed Animals in Southern Italy

2021 ◽  
Vol 9 (8) ◽  
pp. 1656
Author(s):  
Simona Gabrielli ◽  
Marialetizia Palomba ◽  
Federica Furzi ◽  
Emanuele Brianti ◽  
Gabriella Gaglio ◽  
...  
Keyword(s):  
Current Knowledge ◽  
Pcr Amplification ◽  
Epidemiological Studies ◽  
Fallow Deer ◽  
Small Subunit ◽  
Zoonotic Transmission ◽  
Ssu Rrna ◽  
Molecular Approaches ◽  
Wide Range ◽  
Blastocystis Sp

Blastocystis is a common intestinal protist distributed worldwide, infecting humans and a wide range of domestic and wild animals. It exhibits an extensive genetic diversity and, so far, 25 distinct small subunit ribosomal RNA (SSU rRNA) lineages termed subtypes (STs)) have been characterized; among them, 12 have thus far been reported in humans. The aims of the present study were to detect and genetically characterize Blastocystis sp. in synantropic animals to improve our current knowledge on the distribution and zoonotic transmission of Blastocystis STs in Italy. Samples were collected from N = 193 farmed animals and submitted to DNA extraction and PCR amplification of the SSU rRNA. Blastocystis was detected in 60 samples (31.08%) and successfully subtyped. Phylogenetic analysis evidenced that the isolates from fallow deer, goats, and pigs (N = 9) clustered within the ST5; those from pheasants (N = 2) in the ST6; those from chickens (N = 8) in the ST7; those from sheep (N = 6) in the ST10; and those from water buffaloes (N = 9) in the ST14 clade. The comparison between the present isolates from animals and those previously detected in humans in Italy suggested the animal-to-human spillover for ST6 and ST7. The present study represents the widest Blastocystis survey performed thus far in farmed animals in Italy. Further epidemiological studies using molecular approaches are required to determine the occurrence and distribution of Blastocystis STs in other potential animal reservoirs in Italy and to define the pathways of zoonotic transmission.

Genetic variability of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Iran

2012 ◽  
Vol 111 (6) ◽  
pp. 2311-2315 ◽  
Author(s):  
A. Moosavi ◽  
A. Haghighi ◽  
E. Nazemalhosseini Mojarad ◽  
F. Zayeri ◽  
M. Alebouyeh ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Blastocystis Sp ◽  
Asymptomatic Individuals

scholarly journals Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169659 ◽  
Author(s):  
Amandine Cian ◽  
Dima El Safadi ◽  
Marwan Osman ◽  
Romain Moriniere ◽  
Nausicaa Gantois ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Animal Groups ◽  
Zoonotic Risk ◽  
Blastocystis Sp

Two-phase sample preparation and concentration technique for sugar derivatives

1978 ◽  
Vol 50 (8) ◽  
pp. 1226-1227 ◽  
Author(s):  
Maria. Martinez ◽  
David. Nurok ◽  
Albert. Zlatkis
Keyword(s):  
Sample Preparation ◽  
Two Phase ◽  
Concentration Technique ◽  
Phase Sample ◽  
Sugar Derivatives

Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study

2018 ◽  
Author(s):  
Krutanjali Swain ◽  
Abhilash Routray ◽  
Saraswat Sahoo and Subha Ganguly
Keyword(s):  
Cryptosporidium Oocyst ◽  
Buffalo Calves ◽  
Concentration Technique ◽  
Cryptosporidium Oocysts ◽  
Oil Immersion ◽  
Neonatal Diarrhoea ◽  
Conventional Microscopy ◽  
Animal Sciences ◽  
Acid Fast Staining

Bovine cryptosporidiosisis primarily associated with neonatal diarrhoea with higher morbidity than mortality in young calves till they attain immunological maturity. The present investigation relates to a report on the shedding of Cryptosporidium oocyst in two buffalo calves of buffalo farm, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar from 1st days up to 3 month of age at 15 days interval using simple conventional microscopy. By using formol-ether concentration technique followed by modified Ziehl-Neelsen (ZN) acid fast staining, Cryptosporidium oocysts were concentrated and identified. The Cryptosporidium oocysts appeared as reddish pink coloured bodies against a bluish/greenish coloured background at oil immersion using ZN staining kit. The maximum oocyst shedding was observed (2.3 oocyst / field) during 16 to 30 days of age. The oocyst shedding gradually decreased with increase in age afterwards clearly indicating the disease of young buffalo calves.

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