Isolation of Plant Growth Promoting Bacteria from the Rhizosphere of Different Plants and Assessment of Their Plant Growth Promotion Potential

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Anam Yousaf ◽  
Hassan Ahmed Khan ◽  
Tayyaba Younas
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Plant Growth Promoting Bacteria ◽  
Growth Promoting

Plant are benefitted in different aspects by symbiotic bacteria. Environmental conditions, Plantconditions and type of pathogens determine these important services for plants Objective: Theresearch was conducted to assess the plant growth enhancing effects of wheat and cabbagerhizobacteria on the growth of wheat plant Methods: For this purpose, total 49 bacteria were isolatedand characterized from the rhizosphere of wheat and cabbage plants. The isolates were assessed forplant growth promoting properties such as: indole acetic acid production, phosphate solubilization,antibacterial activity and heavy metal resistance. Indole acetic acid was found to be produced by 7isolates and phosphate solubilization was shown by 20 isolates. Antibacterial activity was determinedagainst four clinical isolates like Staphylococcus aureus, Klebsiella sp., Escherichia coli and Pseudomonasaeruginosa Results: Antibacterial activity against Staphylococcus aureus was shown by 38 isolates, 12isolates showed antibacterial activity against Escherichia coli and Klebsiella sp., whereas no isolate wasfound to be positive against Pseudomonas aeruginosa. Another plant growth enhancing trait (heavymetal resistance) was shown by 28 rhizobacteria. In order to evaluate the capability of isolates toenhance the plant growth, bio-inoculation assay was performed using wheat seeds Conclusions:Rhizobacterial inoculation increased the number of roots, shoots, leaves and roots and shoot length ofwheat plantlets as compared to un-inoculated control.

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

scholarly journals Use of termitarium soil as a viable source for biofertilizer and biocontrol

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Seun Owolabi Adebajo ◽  
Pius Olugbenga Akintokun ◽  
Emmanuel Ezaka ◽  
Abidemi Esther Ojo ◽  
Donald Uzowulu Olannye ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phosphate Solubilization ◽  
Soil Samples ◽  
Production Test ◽  
Bacterial Isolates ◽  
Acetic Acid Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background Environmental deterioration arising from the misuse of pesticides and chemical fertilizers in agriculture has resulted in the pursuit of eco-friendly means of growing crop. Evidence has shown that biofertilizers and biocontrol can boost soil fertility and suppress soil pathogens without compromising the safety of the environment. Hence, the study investigated the use of termitarium soil as a viable source for biofertilizer and biocontrol. Results Twenty-seven soil samples were collected from nine different mound soil (household, farm and water bodies in a sterile sample bag). Aliquots of serially diluted samples were plated on nutrient agar, plate count agar, eosin methylene blue agar and MacConkey agar plates. Isolates were identified using standard microbiological techniques. Identified isolates were screened for plant growth-promoting properties using phosphate solubilization test, potassium solubilization test and indole acetic acid production test. Activities of the plant growth-promoting bacteria were carried out using antagonism by diffusible substance method and antagonistic activity of cell-free culture filtrate of bacterial isolates against Ralstonia solanacearum and Fusarium oxysporum. Two hundred bacterial isolates were recovered from the 27 soil samples. The most predominant isolate was Bacillus spp. Out of the 200 bacterial isolates, 57 were positive for phosphate solubilization test, potassium solubilization test and indole acetic acid production test. Out of the 57 isolates, six bacterial isolates had antagonistic activities against Fusarium oxysporum, while seven bacterial isolates antagonized Ralstonia solanacearum. Conclusion The result showed that termite mound soil contains some useful bacteria that are capable of solubilizing phosphate and potassium and producing indole acetic acid which are the plant growth-promoting potentials and as well suppressing plant soil pathogen.

scholarly journals THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Toga Pangihotan Napitupulu ◽  
Atit Kanti ◽  
I Made Sudiana
Keyword(s):  
Plant Growth ◽  
Indole Acetic Acid ◽  
Growth Promotion ◽  
Marginal Land ◽  
Plant Growth Promoting Bacteria ◽  
Atmospheric Nitrogen ◽  
Insoluble Phosphate ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 

scholarly journals Characterization of streptomycetes with potential to promote plant growth and biocontrol

2008 ◽  
Vol 65 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Carla da Silva Sousa ◽  
Ana Cristina Fermino Soares ◽  
Marlon da Silva Garrido
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Indole Acetic Acid ◽  
Extracellular Enzymes ◽  
Root Colonization ◽  
Phosphate Solubilization ◽  
Culture Media ◽  
Growth Promotion ◽  
Salinity Levels

Studies with streptomycetes in biocontrol programs and plant growth promotion are presented as technological alternatives for environmental sustainable production. This work has the objective of characterizing six isolates of streptomycetes aiming the production of extracellular enzymes, indole acetic acid, capacity for phosphate solubilization, root colonization and growth under different pH and salinity levels. For detection of enzyme activity the isolates were grown in culture media with the enzyme substrates as sole carbon source. The root colonization assay was performed on tomato seedlings grown on 0.6% water-agar medium. Growth under different pH and salinity levels was evaluated in AGS medium with 1%, 1.5%, 2%, 2.5%, and 3% NaCl, and pH levels adjusted to 5.0, 5.5, 6.0, 6.5, and 7.0. All isolates produced the enzymes amylase, catalase, and lipase, as well as indole acetic acid. With one exception (AC-92), all isolates presented cellulolytic and chitinolytic activity, and only AC-26 did not show xylanolytic activity. The isolates AC-147, AC-95, and AC-29 were the highest producers of siderophores. The isolates AC-26 and AC-29 did not show capacity for phosphate solubilization. All isolates colonized tomato roots in vitro, and AC-92 grew under all pH and salinity levels tested. The streptomycetes tested were considered as potential biocontrol and plant growth promotion agents.

scholarly journals Screening of plant growth-promoting rhizobacteria from maize (Zea mays) and wheat (Triticum aestivum)

2012 ◽  
Vol 12 (51) ◽  
pp. 6170-6185
Author(s):  
A Karnwal ◽  
Keyword(s):  
Acetic Acid ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Root Elongation ◽  
Indole Acetic Acid ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pot Experiments ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria (PGPR) are free-living soil-borne bacteria that colonize the rhizosphere and have great importance in governing the functional property of terrestrial ecosystems. In this study, rhizospheric bacteria were isolated from maize and wheat and screened for their plant growth promoting activities. These isolates were identified as Pseudomonas , Bacillus , Azospirillum and Azotobacter species. All isolates were tested for their indole acetic acid (IAA) production ability. All isolates produce the varying amount s o f IAA ranging from 0.6- 2.7 μg/ml. The highest concentration of IAA was produced by bacterial strain Bacillus subtilis AK31. A series of growth pouch and pot experiments were conducted to study the effect of bacterial inoculants on the growth of maize and wheat. It was concluded that IAA plays a key role i n the growth promotion of roots in maize and wheat in growth pouch study. In maize, isolate AK1, AK21, AK31 and AK8 showed high indole acetic acid (6.86, 7.11, 7.11 and 7.36 pmol/ml, respectively) and root elongation activity ( 4.10, 5.00, 5.00 and 3.80 cm, respectively) after 96h of growth. In wheat, bacterial strains AK31, AK2, AK14, AK32 and AK15 showed high IAA ( 6.59, 5.66, 5.35, 7.53 and 5.66 pmol/ml, respectively) and root elongation ( 6.07, 4.00, 5.20, 6.90 and 5.20 cm, respectively) activity after 96h of growth. In pot experiments, Bacillus sp. AK21, Bacillus subtilis AK31, Azotobacter diazotrophicus AK14, Microbacterium sp. AK19 and Pseudomonas fluorescens AK32 showed effective results in term s of increase in root and shoot dry weight in maize (123, 130, 121, 120, 124g and 116, 126, 116, 114, 120g/pot, respectively) and wheat (130, 135, 125, 118, 140g and 105, 106, 110, 102, 110g/pot, respectively), in comparison to controls of maize and wheat crops. Thus, it might be concluded that PGPR strains AK21, AK31, AK14, AK19 and AK32 could be used as crop- enhancer and bio- fertilizer for production of maize and wheat .

scholarly journals The Root Microbiome of Salicornia ramosissima as a Seedbank for Plant-Growth Promoting Halotolerant Bacteria

2021 ◽  
Vol 11 (5) ◽  
pp. 2233
Author(s):  
Maria J. Ferreira ◽  
Angela Cunha ◽  
Sandro Figueiredo ◽  
Pedro Faustino ◽  
Carla Patinha ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Cultivated Plants ◽  
Plant Growth Promoting Bacteria ◽  
Crop Cultivation ◽  
Halotolerant Bacteria ◽  
Salicornia Ramosissima ◽  
Plant Growth Promoting ◽  
Growth Promoting

Root−associated microbial communities play important roles in the process of adaptation of plant hosts to environment stressors, and in this perspective, the microbiome of halophytes represents a valuable model for understanding the contribution of microorganisms to plant tolerance to salt. Although considered as the most promising halophyte candidate to crop cultivation, Salicornia ramosissima is one of the least-studied species in terms of microbiome composition and the effect of sediment properties on the diversity of plant-growth promoting bacteria associated with the roots. In this work, we aimed at isolating and characterizing halotolerant bacteria associated with the rhizosphere and root tissues of S. ramosissima, envisaging their application in saline agriculture. Endophytic and rhizosphere bacteria were isolated from wild and crop cultivated plants, growing in different estuarine conditions. Isolates were identified based on 16S rRNA sequences and screened for plant-growth promotion traits. The subsets of isolates from different sampling sites were very different in terms of composition but consistent in terms of the plant-growth promoting traits represented. Bacillus was the most represented genus and expressed the wider range of extracellular enzymatic activities. Halotolerant strains of Salinicola, Pseudomonas, Oceanobacillus, Halomonas, Providencia, Bacillus, Psychrobacter and Brevibacterium also exhibited several plant-growth promotion traits (e.g., 3-indole acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophores, phosphate solubilization). Considering the taxonomic diversity and the plant-growth promotion potential of the isolates, the collection represents a valuable resource that can be used to optimize the crop cultivation of Salicornia under different environmental conditions and for the attenuation of salt stress in non-halophytes, considering the global threat of arable soil salinization.

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

Isolation, identification and characterization of native plant growth promoting bacteria and their plant growth promotion in Zea mays

2021 ◽  
Vol 16 (8) ◽  
pp. 75-80
Author(s):  
Pitchaiah Pelapudi ◽  
Sasikala Ch ◽  
Swarnabala Ganti
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Length ◽  
Growth Promotion ◽  
Shoot Length ◽  
Root Weight ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Shoot Weight

In the present rapid growing world, need for a sustainable agricultural practice which helps in meeting the adequate food demand is much needed. In this context, plant growth promoting bacteria were brought into the spot light by the researchers. Though the plant growth promoting bacteria have several beneficial applications, due to some of the disadvantages in the field conditions, they lagged behind. In the current research work, native PGPR were isolated from the rhizosphere soil samples of maize with an aim to isolate the nitrogen fixing, phosphate solubilising and potash solubilising bacteria. Out of the several isolates, potent PGPR isolates viz., Paenibacillus durus PCPB067, Bacillus megaterium PCBMG041 and Paenibacillus glucanolyticus PCPG051 were isolated and identified by using the 16 S rRNA gene sequencing studies. Genomic DNA sequences obtained were deposited in the NCBI Genbank and accession numbers were assigned as MW793452, MW793456 and MW843633. In order to check the efficacy of the PGPR isolates, pot trials were conducted by taking maize as the host plant. Several parameters viz. shoot length, shoot weight, root length, root weight and weight of the seeds were tested in which PGP treatment showed good results (shoot length - 187±3.5 cm, shoot weight - 31±4 g, root length - 32±3.6 cm, root weight - 17±2 g, yield- 103.3±6.1 g) when compared to the chemical fertilizer treatment (shoot length - 177±3.5 cm, shoot weight - 25±3.6 g, root length - 24±3.5 cm, root weight - 14.6±1.52 g, yield- 85.6±7.6 g). Based on the results, it can be stated that these native PGPR isolates can be effectively used in the plant growth promotion of maize.

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