The effect of chitosan on phenolic compounds, rosmarinic acid and expression of key genes involved in rosmarinic acid biosynthesis in cell suspension culture of Melissa officinalis L.

2021 ◽  
Vol 11 (4) ◽  
pp. 243-261
Author(s):  
A Mahdavianfard ◽  
M Dahajipour Heidarabadi ◽  
KH Malekzadeh ◽  
SR ahhafi
Keyword(s):  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Rosmarinic Acid ◽  
Melissa Officinalis ◽  
Acid Biosynthesis ◽  
Key Genes

scholarly journals Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

2015 ◽  
Vol 68 (4) ◽  
pp. 1415-1424 ◽  
Author(s):  
Amir Sahraroo ◽  
Mohammad Hossein Mirjalili ◽  
Purificación Corchete ◽  
Mesbah Babalar ◽  
Mohammad Reza Fattahi Moghadam
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Rosmarinic Acid

scholarly journals Enhanced Rosmarinic Acid Production by Lavandula vera MM Cell Suspension Culture through Elicitation with Vanadyl Sulfate

2006 ◽  
Vol 61 (3-4) ◽  
pp. 241-244 ◽  
Author(s):  
Milen Georgiev ◽  
Sonya Kuzeva ◽  
Atanas Pavlov ◽  
Elena Kovacheva ◽  
Mladenka Ilieva
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Rosmarinic Acid ◽  
Acid Production ◽  
Culture Medium ◽  
Vanadyl Sulfate ◽  
Lavandula Vera Mm ◽  
Intracellular Content ◽  
Abiotic Elicitor

The influence of elicitation on rosmarinic acid biosynthesis by Lavandula vera MM cell suspension culture was investigated using vanadyl sulfate as an abiotic elicitor. It was established that 12 h after treatment with 25 mg/l vanadyl sulfate the rosmarinic acid production was increased up to 3.92 g/l (2.8 times higher compared to the control cultivation). No significant amounts of rosmarinic acid were detected in the culture medium in comparison with its intracellular content. However, it was observed that the extracellular content of rosmarinic acid is 3.3 times higher compared to the control variant (4 h after treatment at elicitor concentration 25 mg/l).

Elicitation of rosmarinic acid by Lavandula vera MM cell suspension culture with abiotic elicitors

2006 ◽  
Vol 23 (2) ◽  
pp. 301-304 ◽  
Author(s):  
Milen I. Georgiev ◽  
Sonya L. Kuzeva ◽  
Atanas I. Pavlov ◽  
Elena G. Kovacheva ◽  
Mladenka P. Ilieva
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Rosmarinic Acid ◽  
Lavandula Vera Mm ◽  
Abiotic Elicitors

Enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of Daucus carota

Plant Science ◽  
2016 ◽  
Vol 250 ◽  
pp. 154-164 ◽  
Author(s):  
Begoña Miras-Moreno ◽  
Lorena Almagro ◽  
M.A. Pedreño ◽  
Ana Belén Sabater-Jara
Keyword(s):  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Daucus Carota

scholarly journals Rosmarinic Acid Accumulation and Antioxidant Potential of Dracocephalum moldavica L. Cell Suspension Culture

2017 ◽  
Vol 45 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Izabela WEREMCZUK-JEŻYNA ◽  
Izabela GRZEGORCZYK-KAROLAK ◽  
Barbara FRYDRYCH ◽  
Katarzyna HNATUSZKO-KONKA ◽  
Aneta GERSZBERG ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Total Phenolic Content ◽  
Rosmarinic Acid ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic

Dracocephalum moldavica L. (Lamiaceae) is known for its medicinal properties, however greater yields can potentially be achieved by in vitro cultivation. A cell suspension culture of D. moldavica L. (Lamiaceae) derived from root-derived callus was established in liquid MS medium supplemented with 2,4-D 0.5 mg/l and BAP 0.2 mg/l. The biomass and rosmarinic acid (RA) content were analyzed during the 15-day growth cycle of the culture. The highest fresh and dry weight (14.29 g/flask and 1.14 g/flask, respectively) and RA level (27.2 mg/g DW) were reached at day 12 of culture. Methanolic extracts of the culture were assayed for total phenolic content using the Folin-Ciocalteau method, and antioxidant activities using three in vitro tests: ABTS radical scavenging, ferric ion reduction (FRAP) and lipid peroxidation (LPO). RA content and antioxidant potential were found to be higher in cell suspension culture than in root-derived callus. The cell suspension culture also exhibited higher concentrations of RA and ABTS radical scavenging activity than those of the aerial parts of six-month-old field-grown plants of D. moldavica. The overall results show a significant correlation between antioxidant activity, total phenolic content and RA content of the examined extracts. The study presents for the first time the use of cell cultures of D. moldavica for production of therapeutically-valuable metabolites. Our results suggest that the obtained culture could be considered as a potential source of rosmarinic acid, a compound known for its strong antioxidant activity.

scholarly journals Elicitor-Induced Production of Biomass and Pharmaceutical Phenolic Compounds in Cell Suspension Culture of Date Palm (Phoenix dactylifera L.)

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4669
Author(s):  
Jameel Mohammed Al-Khayri ◽  
Poornananda Madhava Naik
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Caffeic Acid ◽  
Cell Suspension Culture ◽  
Date Palm ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Total Phenolic

Plants that synthesize bioactive compounds that have high antioxidant value and elicitation offer a reliable in vitro technique to produce important nutraceutical compounds. The objective of this study is to promote the biosynthesis of these phenolic compounds on a large scale using elicitors in date palm cell suspension culture. Elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2), and silver nitrate (AgNO3) at 50, 100, and 200 mg/L concentrations are used. The effects of elicitors on cell culture were determined in terms of biomass [packed cell volume (PCV), fresh and dry weight], antioxidant activity, and phenolic compounds (catechin, caffeic acid, kaempferol, apigenin) were determined using high-performance liquid chromatography (HPLC). Results revealed that enhanced PCV (12.3%), total phenolic content [317.9 ± 28.7 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)], and radical scavenging activity (86.0 ± 4.5%) were obtained in the 50 mg/L SA treated cell culture of Murashige and Skoog (MS) medium. The accumulation of optimum catechin (26.6 ± 1.3 µg/g DW), caffeic acid (31.4 ± 3.8 µg/g DW), and kaempferol (13.6 ± 1.6 µg/g DW) was found in the 50 mg/L SA-treated culture when compared to the control. These outcomes could be of great importance in the nutraceutical and agronomic industries.

Sign in / Sign up

Export Citation Format

Share Document