scholarly journals Estimation of phytochemicals, antioxidant, antidiabetic and brine shrimp lethality activities of some medicinal plants growing in Nepal

2021 ◽  
Vol 20 (80) ◽  
pp. 102-116
Author(s):  
Junu Kapali ◽  
Khaga Raj Sharma ◽  
◽  
Keyword(s):  
Medicinal Plants ◽  
Brine Shrimp ◽  
Brine Shrimp Lethality

scholarly journals Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants

Proceedings ◽  
2019 ◽  
Vol 41 (1) ◽  
pp. 47
Author(s):  
Sandeep Waghulde ◽  
Mohan K. Kale ◽  
VijayR. Patil
Keyword(s):  
Medicinal Plants ◽  
Brine Shrimp ◽  
Pharmacological Effects ◽  
Bioactive Components ◽  
Pharmacological Activities ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Ethanolic Extracts ◽  
Annona Reticulata

The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of the Aqueous and ethanolic extracts of Annona reticulata with Allium fistolisum and Brassica oleraceaeand correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Ten nauplii were added into three replicates of each concentration of the plant extract. After 24 h the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the extracts of Annona reticulata with Allium fistolisumand Brassica oleraceaewere potent against the brine shrimp when compared alone with combined extracts. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.

Brine shrimp lethality bioassay of selected Indian medicinal plants

Fitoterapia ◽  
2002 ◽  
Vol 73 (6) ◽  
pp. 508-510 ◽  
Author(s):  
R Padmaja ◽  
P.C Arun ◽  
D Prashanth ◽  
M Deepak ◽  
A Amit ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Brine Shrimp ◽  
Brine Shrimp Lethality ◽  
Indian Medicinal Plants

scholarly journals Phytochemical and Cytotoxic Screening of Selected Medicinal Plants (Byrsocarpus coccineus, Terminalia avicennioides and Anogeissus leiocarpus) Using Brine Shrimp (Artemia salina) Lethality Assay

2020 ◽  
pp. 60-71
Author(s):  
C. E. Ukwade ◽  
O. A. T. Ebuehi ◽  
R. A. Adisa
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Brine Shrimp ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Medicinal Uses ◽  
Brine Shrimp Lethality ◽  
Root Extracts ◽  
Anogeissus Leiocarpus ◽  
Mineral Analyses

Three medicinal plants, were investigated based on their ethno-medicinal uses. Byrsocarpus coccineus (B.C), Terminalia avicennioides (T.A) and Anogeissus leiocarpus (A.L) are used traditionally in the treatment of various ailments in Nigeria. Proximate and mineral analyses were carried out on the leaf, stem and root of the three plants. Phytochemical composition and antioxidant activities of the aqueous, ethanol and pet ether (leaf, stem and root) extracts, of the three plants were determined and the extracts were subjected to cytotoxic screening using the in vivo brine shrimp lethality tests. The proximate and mineral analyses show appreciable dietary nutrients in the three plants. Phytochemical analyses of B.C, T.A and A.L (leaf, stem and root) extracts, showed the presence of bioactive compounds, such as alkaloids, tannins, saponins, flavonoids, steroid and phenol. Antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, lipid peroxidation and reducing power), increase in all the plant extracts in a dose dependent manner. The results of brine shrimp lethality tests indicate that plant extracts of B.C, T.A and A.L except A.L stem aqueous extract (130.72 µg/ml), T.A leaf aqueous (130.15 µg/ml) and root aqueous extracts were moderately cytotoxic, while the others were highly cytotoxic. B. coccineus leaf ethanol extract (17.31 µg/ml) was the most cytotoxic. The result shows that B. coccineus leaf ethanol extract has significant antioxidant activity and is cytotoxic to brine shrimp even at low concentration giving credence to its ethno-medicinal uses.

scholarly journals Cytotoxicity Activities of Ethanol Extract of Hooks Uncaria tomentosa West Kalimantan

2020 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Mega Sari Juane Sofiana ◽  
Warsidah Warsidah ◽  
Dodi Iskandar
Keyword(s):  
Medicinal Plants ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Solution Concentration ◽  
Plant Family ◽  
Uncaria Tomentosa ◽  
West Kalimantan ◽  
Brine Shrimp Lethality ◽  
Cytotoxicity Activities

Uncaria tomentosa is a member of the plant family Rubiaceae. It has been used as medicinal plants in West Kalimantan. The cytotoxic of ethanol extract from the hooks of U. Tomentosa was determined. This study used Brine Shrimp Lethality Test (BSLT) method with solution concentration 1,000; 5,000 and 10,000 ppm. The extract has LC50 values of 21,754 ppm. It is indicated the extract not toxic. This extract is potent to be used as drugs.

scholarly journals Brine Shrimp Lethality Bioassay: A Tool for Drug Discovery from Natural Products

2020 ◽  
Vol 1 (2) ◽  
pp. 40-48
Author(s):  
George Owusu-Dapaah ◽  
Nicolas I.Y. Fiagbe
Keyword(s):  
Natural Products ◽  
Drug Discovery ◽  
Medicinal Plants ◽  
Bioactive Compounds ◽  
Brine Shrimp ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Brine Shrimp Lethality ◽  
Structure Activity

This study demonstrates the importance of the Brine Shrimp Bioassay in drug discovery. It uses two medicinal plants, Zanthoxylum xanthoxyloides and Tiliacora funifera. Extracts of Zanthoxylum xanthoxyloides were prepared using water, ethanol, chloroform and diethylether, and each subjected to Brine Shrimp Bioassay. The bioactivities of the extracts were found to be in the order diethylether (LD50=0.958 mg/ml), followed by ethanol (LD50=1.874 mg/ml), water(LD50=2.322 mg/ml), and chloroform (3.518 mg/ml). From Tiliacora funifera is isolated the compound funiferine, which is derivatized to obtain o-methylfuniferine and o-isopropylfuniferine. These were subjected to the brine shrimp bioassay, and the structure –activity relationship (SAR) was determined. The results showed that o-isopropylfuniferine is the most bioactive (LD50 = 9.07 mg/ml), followed by o-methylfuniferine (LD50 =38 mg/ml) with funiferine showing the least activity (LD50 =50.25 mg/ml). The SAR of the compounds indicates that the activity of the compounds increases as the size of the group on the phenolic OH is increased, (i.e. isopropyl (C 3 H7 )> methyl (CH3 ) > hydrogen (H)), which suggests that bulkier groups may give more potent compounds. These two results show that the brine shrimp bioassay can be used as a tool in the search for more bioactive compounds from natural products. Keywords: Brine Shrimp Bioassay, Zanthoxylum xanthoxyloides, Funiferine; o-methylfuniferine,o-isopropylfuniferine.

scholarly journals Antibacterial, Antidiabetic and Brine Shrimp Lethality Activities of Some Selected Medicinal Plants from Kavrepalanchok District of Nepal

2019 ◽  
Vol 24 (1) ◽  
pp. 57-62
Author(s):  
Khaga Raj Sharma ◽  
Rupak Kharel
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Brine Shrimp ◽  
Curcuma Longa ◽  
Antidiabetic Activity ◽  
Diffusion Method ◽  
Toxicity Tests ◽  
Melia Azedarach ◽  
Dependent Manner ◽  
Brine Shrimp Lethality

The methanol extracts of nine medicinal plants collected from Kavrepalanchok district of Nepal were subjected to their potential bioactivity viz. antibacterial, antidiabetic and toxicity tests. Antibacterial property of the extracts was evaluated against Gram-positive and Gram-negative bacteria by agar well diffusion method and antidiabetic activity was investigated by α-amylase inhibition assay. The toxicity of plant extracts was assessed by brine shrimp lethality test. All the nine different plant extracts showed antibacterial activity with the zone of inhibition (ZOI) ranging from 5 to 35 mm. Among the studied plant extracts, Psidium guajava showed the highest ZOI against Salmonella typhi (35 mm) while Melia azedarach was most effective against Staphylococcus aureus (22 mm). The percentage of α- amylase enzyme inhibition was found up to 88.56 ± 3.50 in dose dependent manner. The enzyme inhibitory concentration IC50 value of standard, acarbose was 166.01 μg/mL while the most effective anti-diabetic plant extract of Utrica dioica has 186.67 μg/mL. Moreover, various plant extracts depicted various levels of toxic activities; Curcuma longa demonstrated significant toxicity to Artemia salina with LC50 value 62.10 μg/mL, while Agerantina adenophora, P. guajava and M. azedarach showed moderate toxicity with 103.77, 109.37 and 383.58 μg/mL, respectively.

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