Comparison the cytotoxic effects of Ulva fasciata and Ulva lactuca on the MCF-7 and MDA-MB-231breast cancer cell lines

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 μg/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 μg/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 μg/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 μg/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.

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Gold nanoparticles tethered cinnamic acid: preparation, characterization, and cytotoxic effects on MCF-7 breast cancer cell lines

Applied Nanoscience ◽

10.1007/s13204-018-0764-2 ◽

2018 ◽

Vol 8 (5) ◽

pp. 1133-1138 ◽

Cited By ~ 4

Author(s):

Karthika Subramanian ◽

Kumar Ponnuchamy

Keyword(s):

Breast Cancer ◽

Gold Nanoparticles ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cinnamic Acid ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Cytotoxic Effects ◽

Mcf 7

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Cytotoxicity and Apoptosis Inducing Effects of Some Lathyrane and Tigliane Diterpenes against Breast Cancer Cell Lines

Traditional and Integrative Medicine ◽

10.18502/tim.v6i2.6783 ◽

2021 ◽

Author(s):

Melika Maleki ◽

Zeinab Yazdiniapour ◽

Mustafa Ghanadian ◽

Behzad Zolfaghari ◽

Faezeh Rabbani ◽

...

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Biological Effects ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Cytotoxic Effects ◽

Apoptotic Effects ◽

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Natural compounds and especially herbal medicine are of great interest due to their various biological effects and their potential to act as a drug for the treatment of various neoplasms especially breast cancer that we are facing with its increasing prevalence around the world. The aim of this study was to evaluate the cytotoxic and cell death mechanism of some diterpenoids (Lathyrane or Tigliane) extracted from the Euphorbia sogdiana Popov against two breast cancer cell lines, MCF-7 and 4T1. Determination the cytotoxic effects of four various diterpenoids was performed using MTT assay against MCF-7, 4T1, and HUVEC cell lines. The IC50 of each compound against cell lines was determined by drawing the dose-response graph using graph Pad prism software. Finally, the apoptotic effects of compound with the most cytotoxic effects was determined by flow cytometry assay for 24 hrs of incubation in IC50 concentration. Statistical analysis confirmed compound (3) with the most cytotoxicity against both cancer cell lines. The IC50 of compound (3) was determined as 10.1 ± 5, 28 ± 5, and 50 ± 3 µg/ml, for MCF-7, 4T1, and HUVEC cells, respectively. Furthermore, the cells treated with 5 and 10 μg/ml of compound (3) for 24 hrs, showed 49 and 57% of apoptosis. These surveyed compounds have the potential to be considered as useful anti-breast cancer agents due to the great cytotoxicity and apoptotic effects against related cancer cell lines and safety profile according to their rational selectivity index.

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Synthesis of New Zerumbone Hydrazones and Their In vitro Anticancer Activity

Current Bioactive Compounds ◽

10.2174/1573407216999200630133625 ◽

2020 ◽

Vol 16 ◽

Author(s):

Vu Van Vu ◽

Tran Khac Vu

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Human Cancer Cell ◽

Cytotoxic Effects ◽

Human Cancer Cell Lines ◽

Ic50 Values ◽

Bioassay Result ◽

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Background: A series of new zerumbone hydrazones 5a-f and 9a-f have been synthesized via an in situ procedure in high yields. The structure of synthesized compounds has been confirmed using 1H, 13C NMR and HR-MS. The bioassay result showed that several compounds exhibited cytotoxic effects against three human cancer cell lines including HepG-2, SK-LU-1 and MCF-7. Compound 9a showed the best cytotoxic effect against HepG-2, SK-LU-1 and MCF-7 with IC50 values of 8.20, 6.66 and 9.35 µM, respectively. Objective: This study aims at developing new zerumbone hydrazones as anticancer agents based on zerumbone, a natural compound wildly growing in Vietnam. Method: A series of new zerumbone hydrazones was designed, synthesized and evaluated for cytotoxicity against three human cancer cell lines, including HepG-2, MCF-7 and SKLu-1 using MTT method. Results: The bioassay result showed that several compounds exhibited cytotoxic effects against three human cancer cell lines including HepG-2, SK-LU-1 and MCF-7. Especially, compound 9a displayed the best cytotoxic effect against HepG-2, SK-LU-1 and MCF-7 with IC50 values of 8.20, 6.66 and 9.35 µM, respectively. Results: The bioassay result showed that several compounds exhibited cytotoxic effects against three human cancer cell lines including HepG-2, SK-LU-1 and MCF-7. Especially, compound 9a displayed the best cytotoxic effect against HepG-2, SK-LU-1 and MCF-7 with IC50 values of 8.20, 6.66 and 9.35 µM, respectively. Conclusion: The research results suggest that some compounds could be considered as a lead for future design of zerumbone hydrazones in which bio-isosteric replacements in ortho position of the phenyl ring could be performed to improve the cytotoxic activity.

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ANTIOXIDANT AND CYTOTOXIC EFFECTS OF CURCUMA MANGGA AND BOSENBERGIA ROTUNDA ETHANOLIC EXTRACTS ON MCF-7 CANCER CELL LINES

Science Heritage Journal ◽

10.26480/gws.02.2019.10.14 ◽

2019 ◽

Vol 3 (2) ◽

pp. 10-14

Author(s):

Tamil C.M. Sundram ◽

Mohd Hazim Bin Zakaria ◽

Mohd Hamzah Bin Mohd Nasir

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Cytotoxic Effects ◽

Ethanolic Extracts ◽

Mcf 7

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Abietane Diterpenoids from Clerodendrum trichotomum and Correction of NMR Data of Villosin C and B

Natural Product Communications ◽

10.1177/1934578x1400900706 ◽

2014 ◽

Vol 9 (7) ◽

pp. 1934578X1400900

Author(s):

Linzhen Li ◽

Long Wu ◽

Menghua Wang ◽

Jianbo Sun ◽

Jingyu Liang

Keyword(s):

Circular Dichroism ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Spectroscopic Methods ◽

Cytotoxic Effects ◽

Nmr Data ◽

Abietane Diterpenoids ◽

Circular Dichroïsm ◽

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Nine abietane diterpenoids (1–9) were isolated from the stems of Clerodendrum trichotomum Thunb. and identified by spectroscopic methods. Furthermore, corrected NMR data is provided for Villosin C (1) and B (2) whose absolute configurations were elucidated from circular dichroism (CD) data. All isolates were tested for cytotoxicity against four cancer cell lines (A549, HepG-2, MCF-7 and 4T1). Compounds 1, 2, 3, 4 and 8 were found to have remarkable cytotoxic effects with IC50 values ranging from 8.79 to 35.46 μM.

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In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines

Bioinformation ◽

10.6026/97320630017583 ◽

2021 ◽

Vol 17 (5) ◽

pp. 583-592

Author(s):

Saravanan Rajendrasozhan ◽

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Diethyl Ether ◽

Cancer Cell Lines ◽

Bark Extract ◽

Edible Plant ◽

Cytotoxic Effects ◽

Ether Fraction ◽

Hct 116 ◽

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Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diethyl ether, chloroform, ethyl acetate and butanol fractions). The fractions were examined for their cytotoxicity against human colon cancer (HCT-116 and CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549), hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and prostate cancer (PC-3) cell lines using viability assay. Diethyl ether fraction of Z. spina-christi showed the highest cytotoxic effects among the four extracts of Z. spina-christi. The IC50 of diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2 and 153.8 Ĵg/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa, and HEp-2 cell lines, respectively. Data shows that the diethyl ether fraction of Z. spina-christi showed effective cytotoxic effects in colon, lung and hepatocellular cancer cell lines.

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