Quantification of Riboflavin and Pyridoxine in a Mixed Solution at a High Concentration by Fluorescence Spectrophotometry

2019 ◽  
Vol 41 (6) ◽  
pp. 939-939
Author(s):  
Xiaofei Li Xiaofei Li ◽  
Zhihua Wu Zhihua Wu ◽  
Shenguang Tong Shenguang Tong ◽  
Ping Tong Ping Tong ◽  
Xin Li Xin Li ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Fluorescence Quenching ◽  
Sample Pretreatment ◽  
Mixed Solution ◽  
High Concentration ◽  
Actual Concentration ◽  
Fluorescence Spectrophotometry ◽  
Rp Hplc ◽  
Quenching Efficiency ◽  
Apparent Concentration

A new method of quantifying the contents of riboflavin (RF) and pyridoxine (PY) in their mixed solution was introduced in this study. A mathematical model was established to calculate the actual concentration of PY (Z) based on the apparent concentrations of PY (Y) and RF (X), which were quantified directly when RF and PY were mixed together. First, a linear relationship was found between Y and Z with a high coefficient, which defines fluorescence quenching efficiency. Second, a curvilinear equation was established between the apparent concentration of X and the fluorescence quenching efficiency (k) of PY. The actual concentration of PY could be obtained by using the two equations. The established mathematical model was verified, and the relative error of the calculated PY value was below 2.5%. The upper limit of fluorescence spectrophotometry quantification was up to 20 μg/mL for both RF and PY. Compared with RP-HPLC, this method is convenient in terms of sample pretreatment, as well as saves organic solvents and time.

scholarly journals Detection of nitroaromatic vapours with diketopyrrolopyrrole thin films: exploring the role of structural order and morphology on thin film properties and fluorescence quenching efficiency

2015 ◽  
Vol 51 (6) ◽  
pp. 1143-1146 ◽  
Author(s):  
Monika Warzecha ◽  
Jesus Calvo-Castro ◽  
Alan R. Kennedy ◽  
Alisdair N. Macpherson ◽  
Kenneth Shankland ◽  
...  
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Fluorescence Quenching ◽  
Optical Detection ◽  
Film Properties ◽  
Structural Order ◽  
Quenching Efficiency ◽  
First Time

Sensitive optical detection of nitroaromatic vapours with diketopyrrolopyrrole thin films is reported for the first time.

Phase-Dependent Fluorescence Quenching Efficiency of MoS2 Nanosheets and Their Applications in Multiplex Target Biosensing

2018 ◽  
Vol 10 (49) ◽  
pp. 42009-42017 ◽  
Author(s):  
Lingyi Lan ◽  
Danke Chen ◽  
Yao Yao ◽  
Xinsheng Peng ◽  
Jian Wu ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Mos2 Nanosheets ◽  
Quenching Efficiency

scholarly journals Spreading of chemical substance after its accidental spillage onto the soil surface under unsaturated conditions and variable porosity

2019 ◽  
pp. 41-44
Author(s):  
Olena Kozhushko ◽  
Petro Martyniuk
Keyword(s):  
Mathematical Model ◽  
Soil Moisture ◽  
Soil Profile ◽  
Moisture Transport ◽  
Soil Surface ◽  
Chemical Substance ◽  
High Concentration ◽  
Solute Transfer ◽  
Variable Porosity ◽  
The One

In this paper we study a mathematical model of soil moisture transport with variable porosity. The problem is set for the case of highly concentrated solute spilled onto soil surface. We investigate the way solute transfer, adsorption of contaminant by soil particles and variable porosity influence infiltration of solute into the soil profile. For that purpose, two models are used: a classical one and the one with consideration of mentioned factors. By comparing the results of both models, we established that high concentration of solute causes moisture transport to transpire more slowly, and the pollutant to remain on the soil surface for longer time. Numerical results indicate that porosity can vary considerably under the conditions of intensive contamination with salts.

Cross reactions in radioimmunoassay: a mathematical model for correcting assay results, as exemplified by eliminating the interference of intact thyrotropin in an assay of its beta subunit.

1987 ◽  
Vol 33 (5) ◽  
pp. 658-660 ◽  
Author(s):  
C Dotti ◽  
G P Ceda ◽  
L Denti ◽  
S Cavalieri ◽  
G Borasi ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Conservation Laws ◽  
Regression Line ◽  
Beta Subunit ◽  
Mass Action ◽  
Constant Concentration ◽  
Actual Concentration ◽  
Cross Reactions

Abstract To correct the results of a radioimmunoassay for beta-thyrotropin (TSH) subunit by eliminating the proportion ascribable to intact TSH, we have devised a method that experimentally reproduces the conditions under which the interference develops. Beta-TSH subunit was assayed in several preparations containing known concentrations of both beta-TSH and TSH. The TSH-induced overestimation of beta-TSH was linearly related to the concentration of antigen in the sample. At a constant concentration of TSH, therefore, the following equation is applicable: F = aE + b, where F is the measured (but overestimated) concentration of beta-TSH, E is the actual concentration of beta-TSH, and a and b are the slope and the intercept of the regression line, respectively. a and b, once expressed as a function of TSH, allow the correction of the overestimation. The analysis of the results according to the mass action and conservation laws shows that the antiserum is more avid for the interferent (intact TSH) than for the antigen (beta-TSH).

Improve the fluorescence quenching efficiency of gold nanorod by silver coating

2013 ◽  
Vol 103 (19) ◽  
pp. 193703 ◽  
Author(s):  
Fan Zhang ◽  
Jian Zhu ◽  
Jian-Jun Li ◽  
Jun-Wu Zhao
Keyword(s):  
Fluorescence Quenching ◽  
Gold Nanorod ◽  
Silver Coating ◽  
Quenching Efficiency

Synthesis and Photoluminescence of Eu3+ Doped Sr2CeO4 via Carbonate Precursor

2005 ◽  
Vol 475-479 ◽  
pp. 1181-1184 ◽  
Author(s):  
Shi Kao Shi ◽  
Jun Min Li ◽  
Ji Zhou
Keyword(s):  
Ammonium Carbonate ◽  
Europium Nitrate ◽  
Mixed Solution ◽  
High Concentration ◽  
Efficient Energy Transfer ◽  
Phase Purity ◽  
Efficient Energy ◽  
Different Temperatures ◽  
Carbonate Precursor ◽  
Emission Light

Carbonate precursor of Eu3+ doped Sr2CeO4 is obtained from a mixed solution of strontium, cerium and europium nitrate using ammonium carbonate as precipitant. Calcining the carbonate precursor at different temperatures, the Eu3+ doped Sr2CeO4 phosphor is synthesized. The phase purity of the material is closely related with the calcination temperature, and the phosphor with high purity can be achieved after the precursor annealed at 1200°C. The photoluminescence performance for Eu3+ doped Sr2CeO4 depends on the concentration of Eu3+. The excitation and emission reveal efficient energy transfer from Ce4+ to Eu3+, making the emission light from blue-white to red-white, further to red as the Eu3+ concentration gradually changes from low concentration (1 mol%) to high concentration (10 mol%).

A Novel Synthesis of CaWO4:Eu3+ Flower Like Microcrystals in Ethanol/Water Mixed System and Characterization

2012 ◽  
Vol 531-532 ◽  
pp. 512-518 ◽  
Author(s):  
Ye Qing Chen ◽  
Joo Hyun Lee ◽  
Sung Wook Park ◽  
Byung Kee Moon ◽  
Byung Chun Choi ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Hydrothermal Process ◽  
Volume Ratio ◽  
Mixed System ◽  
Mixed Solution ◽  
Photoluminescence Properties ◽  
X Ray Diffraction ◽  
High Concentration ◽  
X Ray ◽  
Xrd Patterns

In this paper, we report a successful synthesis of CaWO4:Eu3+ phosphor via an ethanol assisted hydrothermal process. X-ray diffraction (XRD) patterns, X-ray photoelectron spectroscopy (XPS) and field emission scanning electron microscope (FE-SEM) were used to investigate the growth of the products. The water and ethanol volume ratio is found to have extraordinary effect on the particle size and morphological appearance. Flower like ~ 1µm superstructures can be obtained with mixed solution of w/e of 50/50 at 120 °C hydrothermal sysnthesis for 12 h. High concentration of ethanol in aqueous solution was discovered to have a tendency in limiting the interaction between the small particles for crystallization. Temperature and time experiments were also performed to further investigate the growth mechanism of the ethanol assisted hydrothermal process. The photoluminescence properties of flower like CaWO4:Eu3+ has also been investigated.

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