scholarly journals Antioxidant and Anti-inflammatory Effects of Fermented Eggplant Fruit Extracts with Leuconostoc mesenteroides KD20

2021 ◽  
Vol 27 (5) ◽  
pp. 1278-1285
Author(s):  
Bum-Chun Lee ◽  
Hee-Sook Kim
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Inhibitory Effect ◽  
Ultraviolet B ◽  
Fermented Foods ◽  
Skin Protection ◽  
Skin Damage ◽  
Fruit Extract ◽  
Protection Effect ◽  
Ultraviolet Ray ◽  
Traditional Fermented Foods

We isolated Leuconostoc mesenteroides KD20, which has its application as a cosmetic material, while searching for useful microbes in Gangwon-do’s traditional fermented foods. Eggplant fruit extract (EG) was fermented with lactic acid bacteria L. mesenteroides. HPLC analysis results for the component analysis of EG and the fermented eggplant fruit extract (FEG) showed bio-conversion of quercetin 3-O-β-D-glucoside and rutin. To investigate skin protection effects of EG and FEG against skin damage by ultraviolet ray, we measured antioxidation and inhibitory effect of inflammation-related cytokines in keratinocytes irradiated with ultraviolet B (UVB). Biosynthesis of IL- 1α, which is increased with UVB in keratinocytes, was inhibited by 24.7% and 28.3% at 100 μg/ml of EG and FEG, respectively. Biosynthesis of PGE2 in keratinocytes was inhibited by 45.7% and 52.3% at 100 μg/ml of EG and FEG, respectively, indicating a greater effect of FEG. Therefore, EG and FEG can be used as an excellent material for cosmetics with skin protection effect against ultraviolet irradiation in keratinocytes.

Inhibitory Effects of Baicalin on Ultraviolet B-Induced Photo-Damage in Keratinocyte Cell Line

2008 ◽  
Vol 36 (04) ◽  
pp. 745-760 ◽  
Author(s):  
Wei Min ◽  
Xiang-Fei Lin ◽  
Xu Miao ◽  
Bao-Tao Wang ◽  
Zi-Liang Yang ◽  
...  
Keyword(s):  
Clinical Medicine ◽  
Protein A ◽  
Inhibitory Effect ◽  
Ultraviolet B ◽  
Nuclear Antigen ◽  
Skin Damage ◽  
Protein Levels ◽  
Initial Event ◽  
Pyrimidine Dimers ◽  
Photo Damage

Baicalin, one kind of Chinese herbal medicine with anti-inflammatory and anti-oxidant property, has been commonly used as a clinical medicine. However, little has been known about the effects of Baicalin on ultraviolet (UV) induced photo-aging and photo-carcinogenesis. The photoproduct is critical to the initial event of UV-induced photo-carcinogenesis. The purpose of the present study was to investigate whether Baicalin, in immortalized human keratinocyte HaCaT cells, could inhibit ultraviolet-B (UVB) induced skin damage and its possible underlying mechanisms, such as inhibiting UVB-induced cytotoxicity and apoptosis, cyclobutane pyrimidine dimers (CPDs), down-regulating the expression of regulatory proteins which are related to cell apoptosis and DNA damage/repair. Our study revealed that Baicalin treatment could inhibit the UVB-induced cytotoxicity, apoptosis and CPD level. It also decreased the mRNA expression of apoptosis-regulatory genes (p53-p21 and c-fos), the protein levels of p53, proliferating cell nuclear antigen (PCNA) and repair protein A (RPA), and the secretion of cytokines [interleukin(IL)-6 and tumor necrosis factor (TNF-α)]. These results suggested that Baicalin may have an inhibitory effect on the UVB-induced photo-damage by blocking the relevant cytokine secretion and expression of p53-p21, c-fos, PCNA and RPA genes.

Effect of Epigallocatechingallate on Ultraviolet B-Induced Photo-Damage in Keratinocyte Cell Line

2006 ◽  
Vol 34 (05) ◽  
pp. 911-922 ◽  
Author(s):  
Dan Luo ◽  
Wei Min ◽  
Xiang-Fei Lin ◽  
Di Wu ◽  
Yang Xu ◽  
...  
Keyword(s):  
Regulatory Gene ◽  
Inhibitory Effect ◽  
Ultraviolet B ◽  
Skin Damage ◽  
Protective Mechanisms ◽  
Egcg Treatment ◽  
Keratinocyte Cell ◽  
Cellular Apoptosis ◽  
Anti Oxidant ◽  
Photo Damage

One type of traditional Chinese medicines, epigallocatechingallate (EGCG) has been commonly used as a clinical and skin health protective ingredient. It has been known to have photo-protective, anti-inflammatory, and anti-oxidant effects. However, little is known about the mechanisms of EGCG on UV-induced photo-aging and photo-carcinogenesis. In the present study, we investigated the photo-protective mechanisms of EGCG on UVB-induced skin damage, including the potency of EGCG to inhibit the UVB-induced cytotoxicity, secretion of cytokine (IL-6 and TNF-α), cellular apoptosis, expression of apoptosis-regulatory genes (p53–p21) and c-fos gene in cultured immortalized human keratinocyte HaCaT cells. EGCG treatment decreased UVB- induced cell cytotoxicity and apoptosis. It also inhibited the mRNA expressions of apoptosis-regulatory gene (p53 and p21) and c-fos gene. These results suggest that EGCG may have an inhibitory effect on UVB-induced photo-damage and apoptosis by blocking the cytokine secretion and the mRNA expressions of p53, p21 and c-fos genes.

scholarly journals Coenzyme Q10 Sunscreen Prevents Progression of Ultraviolet-Induced Skin Damage in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Haiyou Wu ◽  
Zhangfeng Zhong ◽  
Sien Lin ◽  
Chuqun Qiu ◽  
Peitao Xie ◽  
...  
Keyword(s):  
Coenzyme Q ◽  
Rapid Development ◽  
Mouse Skin ◽  
Skin Surface ◽  
Positive Control ◽  
Ultraviolet B ◽  
Control Group ◽  
Skin Damage ◽  
Ultraviolet Ray ◽  
Underlying Mechanisms

The level of sun ultraviolet ray reaching the surface of the earth is increasing severely due to the rapid development of the society and environmental destruction. Excessive exposure to ultraviolet radiation causes skin damage and photoaging. Therefore, it is emerged to develop effective sunscreen to prevent ultraviolet-induced skin damage. This study was aimed at investigating the effects of Coenzyme Q10 (CoQ10) sunscreen on the prevention of ultraviolet B radiation- (UVB-) induced mouse skin damage. Three-month-old female mice were used, and they were randomly divided into four groups: control, model, CoQ10, and titanium dioxide (TiO2; positive control) groups. Our results showed that body weight, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, and DNA (cytosine-5)-methyltransferase 1 (DNMT1) protein expression were significantly decreased, while malondialdehyde (MDA) activity and metalloproteinase-1 (MMP-1) level were increased in UVB-treated mice. Besides, the stratum corneum was shed from the skin surface in the model group compared with the control group. In contrast, CoQ10 sunscreen prevented from UVB-induced skin damage, as well as reversing SOD, GSH-Px, and MDA activities, and MMP-1 and DNMT1 levels. Taken together, the current study provided further evidence on the prevention of UVB-induced skin damage by CoQ10 and its underlying mechanisms.

Research Trend in Traditional Fermented Foods Focused on Health Functional Evaluation

2018 ◽  
Vol 47 (4) ◽  
pp. 373-386 ◽  
Author(s):  
Eun-Gyung Mun ◽  
Bohkyung Kim ◽  
Eun-Young Kim ◽  
Hae-Jeung Lee ◽  
Young Kim ◽  
...  
Keyword(s):  
Research Trend ◽  
Fermented Foods ◽  
Functional Evaluation ◽  
Traditional Fermented Foods

scholarly journals UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eui Jeong Han ◽  
Seo-Young Kim ◽  
Hee-Jin Han ◽  
Hyun-Soo Kim ◽  
Kil-Nam Kim ◽  
...  
Keyword(s):  
Skin Barrier ◽  
Human Keratinocytes ◽  
Underlying Mechanism ◽  
Ultraviolet B ◽  
Sargassum Horneri ◽  
Cellular Damage ◽  
Protective Effects ◽  
Skin Damage ◽  
Antioxidant Mechanism ◽  
Induced Apoptosis

AbstractThe present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

scholarly journals Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced Damage in HaCaT Cells

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 273
Author(s):  
Young Ah Jang ◽  
Bo Ae Kim
Keyword(s):  
Antioxidant Defense System ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Control Group ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Blue Green Algae ◽  
Radiation Group ◽  
Skin Wrinkles

Background and objectives: Reactive oxygen species (ROS) overwhelm the antioxidant defense system, induce oxidative stress, and increase matrix metalloproteinase (MMP) expression, resulting in skin aging. Thus, preventing ultraviolet B (UVB)-induced skin damage can attenuate skin aging. Spirulina (a biomass of cyanobacteria, also called blue-green algae) is comprised of prokaryotes, whereas microalgae are eukaryotes and are rich in phycocyanin, a powerful antioxidant. Materials and Methods: Here, we investigated the photoprotective effects of spirulina-derived C-phycocyanin (C-PC) against UVB radiation using keratinocytes (HaCaT cells). Results: UVB radiation increased MMP-1 and MMP-9 expression but decreased involucrin, filaggrin, and loricrin expression. C-PC showed no toxicity at concentrations of 5–80 μg/mL in terms of HaCaT cell viability. UVB-irradiated HaCaT cells had a 50.8% survival rate, which increased to 80.3% with C-PC treatment. MMP expression increased with UVB treatment, whereas MMP-1 and MMP-9 concentrations decreased with C-PC treatment. UVB reduced involucrin, filaggrin, and loricrin expression in HaCaT cells, but 80 μg/mL C-PC increased their expression by >25%. In the UVB radiation group, dichlorofluorescin diacetate fluorescence intensity in HaCaT cells increased by 81.6% compared with that in the control group, whereas ROS production was reduced by 51.2% and 55.1% upon treatment with 40 and 80 μg/mL C-PC, respectively. Conclusions: C-PC might reduce or prevent skin aging by reducing UVB irradiation-induced skin wrinkles and free radicals.

scholarly journals Effects of Porcine Placenta Extract Ingestion on Ultraviolet B-induced Skin Damage in Hairless Mice

2015 ◽  
Vol 35 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Ki-Bae Hong ◽  
Yooheon Park ◽  
Jae Hwan Kim ◽  
Jin Man Kim ◽  
Hyung Joo Suh
Keyword(s):  
Ultraviolet B ◽  
Skin Damage ◽  
Hairless Mice

Isolation, Identification and Characterization of Beneficial Microorganisms from Traditional Fermented Foods

2022 ◽  
pp. 14-56
Author(s):  
Phu‐Ha Ho ◽  
Tuan‐Anh Pham ◽  
Quoc‐Phong Truong ◽  
Lan‐Huong Nguyen ◽  
Tien‐Thanh Nguyen ◽  
...  
Keyword(s):  
Fermented Foods ◽  
Beneficial Microorganisms ◽  
Identification And Characterization ◽  
Traditional Fermented Foods
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