scholarly journals Coenzyme Q10 Sunscreen Prevents Progression of Ultraviolet-Induced Skin Damage in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Haiyou Wu ◽  
Zhangfeng Zhong ◽  
Sien Lin ◽  
Chuqun Qiu ◽  
Peitao Xie ◽  
...  
Keyword(s):  
Coenzyme Q ◽  
Rapid Development ◽  
Mouse Skin ◽  
Skin Surface ◽  
Positive Control ◽  
Ultraviolet B ◽  
Control Group ◽  
Skin Damage ◽  
Ultraviolet Ray ◽  
Underlying Mechanisms

The level of sun ultraviolet ray reaching the surface of the earth is increasing severely due to the rapid development of the society and environmental destruction. Excessive exposure to ultraviolet radiation causes skin damage and photoaging. Therefore, it is emerged to develop effective sunscreen to prevent ultraviolet-induced skin damage. This study was aimed at investigating the effects of Coenzyme Q10 (CoQ10) sunscreen on the prevention of ultraviolet B radiation- (UVB-) induced mouse skin damage. Three-month-old female mice were used, and they were randomly divided into four groups: control, model, CoQ10, and titanium dioxide (TiO2; positive control) groups. Our results showed that body weight, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, and DNA (cytosine-5)-methyltransferase 1 (DNMT1) protein expression were significantly decreased, while malondialdehyde (MDA) activity and metalloproteinase-1 (MMP-1) level were increased in UVB-treated mice. Besides, the stratum corneum was shed from the skin surface in the model group compared with the control group. In contrast, CoQ10 sunscreen prevented from UVB-induced skin damage, as well as reversing SOD, GSH-Px, and MDA activities, and MMP-1 and DNMT1 levels. Taken together, the current study provided further evidence on the prevention of UVB-induced skin damage by CoQ10 and its underlying mechanisms.

scholarly journals Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced Damage in HaCaT Cells

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 273
Author(s):  
Young Ah Jang ◽  
Bo Ae Kim
Keyword(s):  
Antioxidant Defense System ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Control Group ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Blue Green Algae ◽  
Radiation Group ◽  
Skin Wrinkles

Background and objectives: Reactive oxygen species (ROS) overwhelm the antioxidant defense system, induce oxidative stress, and increase matrix metalloproteinase (MMP) expression, resulting in skin aging. Thus, preventing ultraviolet B (UVB)-induced skin damage can attenuate skin aging. Spirulina (a biomass of cyanobacteria, also called blue-green algae) is comprised of prokaryotes, whereas microalgae are eukaryotes and are rich in phycocyanin, a powerful antioxidant. Materials and Methods: Here, we investigated the photoprotective effects of spirulina-derived C-phycocyanin (C-PC) against UVB radiation using keratinocytes (HaCaT cells). Results: UVB radiation increased MMP-1 and MMP-9 expression but decreased involucrin, filaggrin, and loricrin expression. C-PC showed no toxicity at concentrations of 5–80 μg/mL in terms of HaCaT cell viability. UVB-irradiated HaCaT cells had a 50.8% survival rate, which increased to 80.3% with C-PC treatment. MMP expression increased with UVB treatment, whereas MMP-1 and MMP-9 concentrations decreased with C-PC treatment. UVB reduced involucrin, filaggrin, and loricrin expression in HaCaT cells, but 80 μg/mL C-PC increased their expression by >25%. In the UVB radiation group, dichlorofluorescin diacetate fluorescence intensity in HaCaT cells increased by 81.6% compared with that in the control group, whereas ROS production was reduced by 51.2% and 55.1% upon treatment with 40 and 80 μg/mL C-PC, respectively. Conclusions: C-PC might reduce or prevent skin aging by reducing UVB irradiation-induced skin wrinkles and free radicals.

scholarly journals Laminarin Attenuates Ultraviolet-Induced Skin Damage by Reducing Superoxide Anion Levels and Increasing Endogenous Antioxidants in the Dorsal Skin of Mice

Marine Drugs ◽  
2020 ◽  
Vol 18 (7) ◽  
pp. 345 ◽  
Author(s):  
Ji Hyeon Ahn ◽  
Dae Won Kim ◽  
Cheol Woo Park ◽  
Bora Kim ◽  
Hyejin Sim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Anion ◽  
Collagen Fibers ◽  
Ultraviolet B ◽  
Control Group ◽  
Dorsal Skin ◽  
Skin Damage ◽  
Superoxide Anion Production ◽  
Uvb Exposure ◽  
Immunohistochemical Studies

A number of studies have demonstrated that marine carbohydrates display anti-oxidant, anti-melanogenic, and anti-aging activities in the skin. Laminarin (LA), a low-molecular-weight polysaccharide, is found in brown algae. The benefits of LA in ultraviolet B (UVB) induced photodamage of the skin have not been reported. The aim of this study was to investigate the effects of pre-treated LA on histopathological changes and oxidative damage in mouse dorsal skin on day 5, following repeated UVB exposure. Histopathology, Western blot analysis and immunohistochemical studies showed that epidermal thickness in the UVB group was significantly increased; however, the thickness in the UVB group treated with LA (LA/UVB group) was less compared with that of the UVB group. Collagen fibers in the dermis of the UVB group were significantly decreased and destroyed, whereas, in the LA/UVB group, the density of collagen fibers was significantly increased compared with that of the UVB group. Oxidative stress due to superoxide anion production measured via dihydroethidium fluorescence staining was dramatically increased in the UVB group, whereas in the LA/UVB group, the oxidative stress was significantly decreased. Expressions of SOD1, glutathione peroxidase and catalase were markedly reduced in the UVB group, whereas in the LA/UVB group, they were significantly higher along with SOD2 than in the control group. Taken together, our results indicate that LA pretreatment prevents or attenuates skin damage, by decreasing oxidative stress and increasing antioxidant enzymes in mouse dorsal skin.

scholarly journals Protective effect of total flavonoids from boxthorn leaf against UVB irradiation-induced skin injury

2021 ◽  
Vol 18 (9) ◽  
pp. 1943-1947
Author(s):  
Qing Yu ◽  
Ying Shen ◽  
Yadan Gan ◽  
Liang Zheng
Keyword(s):  
Protective Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Ultraviolet B ◽  
Control Group ◽  
Total Flavonoids ◽  
Skin Damage ◽  
Skin Injury ◽  
Uvb Irradiation ◽  
Base Group

Purpose: To investigate the protective effect of total flavonoids from boxthorn leaf against skin injury induced by UVB irradiation, and to elucidate the underlying mechanism of action. Method: Healthy female mice (n = 100) were randomly divided into four groups: normal control group, UV negative control group, cream base group, and boxthorn leaf total flavonoid (BLTF) group, with 25 mice in each group. The mice in each group were irradiated with ultraviolet B (UVB) irradiation instrument for 1.5 h daily for 3 weeks. Mice in the cream base group were smeared with cream base on their backs, while mice in BLTF group were smeared with 15 mg/g boxthorn BLTF cream. The control and negative control group mice were not treated. Changes in superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels were determined using standard methods. Results: Compared to the negative control group, the levels of SOD and GSH-Px in the control group and BLTF were significantly elevated, while MDA levels declined significantly (p < 0.05). Although higher GSH-Px and SOD levels, and lower MDA were seen in the cream base group than in negative control group, these indices were comparable for the two groups (p > 0.05). Conclusion: The total flavonoids of boxthorn leaves improve resistance to UVB-induced skin damage by regulating SOD, MDA and GSH-Px levels in the skin of mice. Thus, they exert protective effects on the skin.

scholarly journals Antioxidant and Anti-inflammatory Effects of Fermented Eggplant Fruit Extracts with Leuconostoc mesenteroides KD20

2021 ◽  
Vol 27 (5) ◽  
pp. 1278-1285
Author(s):  
Bum-Chun Lee ◽  
Hee-Sook Kim
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Inhibitory Effect ◽  
Ultraviolet B ◽  
Fermented Foods ◽  
Skin Protection ◽  
Skin Damage ◽  
Fruit Extract ◽  
Protection Effect ◽  
Ultraviolet Ray ◽  
Traditional Fermented Foods

We isolated Leuconostoc mesenteroides KD20, which has its application as a cosmetic material, while searching for useful microbes in Gangwon-do’s traditional fermented foods. Eggplant fruit extract (EG) was fermented with lactic acid bacteria L. mesenteroides. HPLC analysis results for the component analysis of EG and the fermented eggplant fruit extract (FEG) showed bio-conversion of quercetin 3-O-β-D-glucoside and rutin. To investigate skin protection effects of EG and FEG against skin damage by ultraviolet ray, we measured antioxidation and inhibitory effect of inflammation-related cytokines in keratinocytes irradiated with ultraviolet B (UVB). Biosynthesis of IL- 1α, which is increased with UVB in keratinocytes, was inhibited by 24.7% and 28.3% at 100 μg/ml of EG and FEG, respectively. Biosynthesis of PGE2 in keratinocytes was inhibited by 45.7% and 52.3% at 100 μg/ml of EG and FEG, respectively, indicating a greater effect of FEG. Therefore, EG and FEG can be used as an excellent material for cosmetics with skin protection effect against ultraviolet irradiation in keratinocytes.

scholarly journals Coix Seed Diet Ameliorates Immune Function Disorders in Experimental Colitis Mice

Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 123
Author(s):  
Qilyu Zhou ◽  
Ruyang Yu ◽  
Tianlong Liu ◽  
Yeye Li ◽  
Jia Zhong ◽  
...  
Keyword(s):  
Immune Cell ◽  
Complete Blood Count ◽  
Experimental Colitis ◽  
Positive Control ◽  
Network Pharmacology ◽  
Control Group ◽  
Group Model ◽  
Colon Tissue ◽  
Coix Seed ◽  
Underlying Mechanisms

Coix seed is a functional food in the Chinese diet that possesses the ability to alleviate ulcerative colitis clinically. However, the underlying mechanisms remain ambiguous. In this study, we investigated the protective effect of the Coix seed diet on experimental colitis mice. The mice were randomly divided into four groups: control group, model group, Coix seed feed group, and positive control group. The maintenance feed of the mice was replaced with Coix seed feed 10 days before orally administering the mice 5% (w/v) dextran sulfate sodium drink. As a result, the Coix seed feed alleviated colitis symptoms, maintained the complete blood count at a normal level, reduced the pathological score, relieved inflammatory cytokine secretion, and alleviated oxidative stress. Network pharmacology analysis was used for further exploration of the targets of Coix seed feed. The results showed that T-cell regulation is one of the targets of Coix seed feed, and the analysis of the T-lymphocyte subset and innate immune cell distribution of the colon tissue supported the network pharmacology results. In conclusion, Coix seed, as a staple food, can alleviate experimental colitis, and the mechanism may be related to the immune regulation effect of Coix seeds.

scholarly journals POTENSI EKSTRAK ETANOL BAWANG MERAH (Allium ascolonicum L.) DAN GARAM NaCl MENURUNKAN LUAS AREA SERTA MENINGKATKAN KONTRAKSI JARINGAN LUKA BAKAR RINGAN

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 53
Author(s):  
I M. Sukadana ◽  
S. Rahayu ◽  
Melli Melli
Keyword(s):  
Treatment Group ◽  
Subcutaneous Tissue ◽  
Healing Process ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Skin Damage ◽  
Control Group Design ◽  
Onion Extract ◽  
Skin Response

Burns is a skin response and subcutaneous tissue to temperature or thermal trauma that affects skin damage. The weight or severity of tissue damage from burns is influenced by several factors including deep burns, burns area, burn location, general body health, injury mechanism and age. The area of ??burns and the percentage of wound contractions is an indicator of the healing process of minor burns. This study used 25 wistar rats divided into 5 treatment groups with randomized posttest only control group design as follows; K: given vaseline treatment (negative control), Kp: given bioplacenton treatment (positive control), P1: given salt treatment at 7.5% concentration in vaseline, P2: given onion extract treatment at 40% concentration in vaseline, and P3: given a mixture of onion extract 40% and kitchen salt at a concentration of 7,5% in vaseline. The result of One-Way ANOVA and Post Hoc Test LSD using SPSS for Windows version 19 shows P2 treatment group is onion extract of 40% give the best result concentration of onion extract so it is very potential to be developed further as light burning agent compared to P1 and P3 treatment group because of its ability to decrease the area of ??340,79 mm2 on the first day to 11,75 mm2 and increased wound contraction by 81,59% at the end of treatment

scholarly journals ACHATINA FULICA MUCUS ATTENUATES ULTRAVIOLET B-INDUCED FIBROBLAST PHOTOAGING THROUGH REDUCING INFLAMMATION, ANGIOGENESIS, AND MATRIX METALLOPROTEINASE

2020 ◽  
pp. 199-203
Author(s):  
CH. TRI NURYANA ◽  
SOFIA MUBARIKA ◽  
YOHANES WIDODO WIROHADIDJOJO ◽  
NUR ARFIAN ◽  
PUTU MEGA ADITYA DEVI AYU MARA ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Platelet Rich Plasma ◽  
Quantitative Polymerase Chain Reaction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Dermal Fibroblasts ◽  
Ultraviolet B ◽  
Control Group ◽  
Achatina Fulica

Objective: This study aimed to observe the effects of Achatina fulica mucus (AFM) on ultraviolet B (UVB)-induced fibroblast photoaging by assessing monocyte chemotactic protein (MCP)-1, vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-3, and MMP-12 mRNA expressions. Methods: Cell cultures of normal human dermal fibroblasts (NHDFs) were divided into six groups: Group 1 was normal fibroblasts without UVB irradiation as normal control and Groups 2–5 consisted of 100 mJ/cm2 UVB-induced aged fibroblasts. Group 2 had no treatment as negative control, Group 3 was treated by platelet-rich plasma 10% as positive control group, and Groups 4–6 were treated by various concentrations of AFM (3.9 μl, 15.625 μl, and 62.5 μl). The MCP-1, VEGF, MMP-3, and MMP-12 mRNA expressions in the different NHDF groups were assessed by quantitative polymerase chain reaction. Results: The mRNA expressions of MCP-1, VEGF, MMP-3, and MMP-12 in the AFM group compared to the UVB group decreased 8, 5, 5, and 4 folds, respectively. AF62 exhibited the highest improvement among the other AFM-treated groups. Conclusion: AFM treatment attenuates UVB-induced fibroblasts photoaging by reducing inflammation, angiogenesis, and matrix metalloproteinases.

scholarly journals Assessment of Skin Irritation and Sensitisation Effects by Topical Pterostilbene

2021 ◽  
Vol 14 (4) ◽  
pp. 1917-1927
Author(s):  
Ahmad Rohi Ghazali ◽  
Nor Fadilah Rajab ◽  
Muhammad Firas Zainuddin ◽  
Nazeha Ahmat
Keyword(s):  
Skin Irritation ◽  
Skin Surface ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Uvb Radiation ◽  
Dermal Toxicity ◽  
Dermal Irritation ◽  
Skin Irritation Test

Pterostilbene has dermal medicinal benefits such as anti-inflammatory, antioxidative effects and photoprotective properties against UVB radiation. The purpose of this study was to evaluate the dermal toxicity of pterostilbene via skin irritation and sensitisation. A skin irritation test was done according to the Organization Economic Co-operation and Development 404 guideline with the scoring of irritation based on erythema and oedema in 5 albino rabbits were observed up to 14 days. The sensitisation test using the Buehler Test in accordance with the ISO 10993-10 guideline was used to study the sensitisation effect of pterostilbene on the skin surface of albino guinea pigs. According to the primary dermal irritation index (PDII), the positive control group was classified with severe irritation (scorings of 7.71). No irritation was observed for the negative control and the 5% pterostilbene treated groups. But, a slight irritation reaction with PDII scorings of 0.86 was observed in the 10% pterostilbene treated group. The sensitisation study indicated that pterostilbene did not produce any sensitisation signs, thus classified as a non-sensitiser agent according to the Magnusson & Kligman classification. Pterostilbene-treated skin also did not indicate any signs of irritation and sensitisation. In conclusion, pterostilbene did not cause dermal toxicity upon application on the skin.

Utilization of Tofu Dregs Flour in Making Snack Bars for People with Diabetes Mellitus

2020 ◽  
Vol 7 (1) ◽  
pp. 27
Author(s):  
Ni Putu Diah Cahyani Subamia ◽  
Komang Ayu Nocianitri ◽  
I Dewa Gede Mayun Permana
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Wheat Flour ◽  
Sugar Content ◽  
Type Ii Diabetes Mellitus ◽  
Positive Control ◽  
Water Soluble ◽  
Control Group ◽  
Unhealthy Lifestyle ◽  
Control Group Design

Type II diabetes mellitus due to an unhealthy lifestyle, one of which is the lack of fiber in daily food consumption. One food that has a high fiber content is tofu dregs. The purpose of this study was to determine substitution of wheat flour with tofu dregs flour to produce a snack bar with the best characteristics, and determine the effect of consumption of snack bar from tofu dregs on blood sugar content in rats. The research was conducted two steps. Step I: Formulation of snack bar using a completely randomized design with tofu dregs flour concentration of 0 %, 10 %, 20 %, 30 %, 40 %, and 50 %. The variables of this study were the content of water, ash, protein, fat, carbohydrates, sensory tests, and effectiveness tests. Step II: the best characteristic snack bar in the step I was used experimental rats. This step used true experimental design with pre-post test control group design. The variables of study were blood glucose levels before treatment and after treatment. The treatment group consisted of normal, negative, positive control, and snack bar. The results of the first step of the research showed that substitution of wheat flour with 40 % tofu dregs produced the best characteristic snack bar with 17.19 % water content, 1.33 % ash content, 11.03 % protein, 20.53 % fat, 49.92 % carbohydrate, light brown color, unpleasant aroma, distinctive soy taste, crumb texture, 0.63 % water soluble food fiber, 1.57 % water insoluble fiber, and total food fiber 2.36 %. The results of the second step of the study showed that the provision of substitution of wheat flour with tofu dregs flour 40 % could reduce blood sugar levels in diabetic mellitus rats until normal, start 290 mg/dl to 108.5 mg/dl. Tofu dregs flour can be used for snack bar formulations for people with diabetes mellitus.

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