scholarly journals OPTIMIZATION OF THE WASTE PROCESSING PROCEDURE OF THE BEER INDUSTRY AND OBTAINING LIPID PREPARATION FROM YEAST BIOMASS

Akademos ◽  
2021 ◽  
Vol 60 (1) ◽  
pp. 51-56
Author(s):  
Elena Tofan ◽  
◽  
Natalia Chiselita ◽  
Oleg Chiselita ◽  
Alina Besliu ◽  
...  
Keyword(s):  
Sodium Phosphate ◽  
Lipid Fraction ◽  
Waste Processing ◽  
Acidic Solutions ◽  
Sodium Phosphate Buffer ◽  
Beer Industry ◽  
Yeast Biomass ◽  
Processing Procedure ◽  
Extraction Stage ◽  
By Products

Currently, special attention is paid to the use of industrial by-products, in particular those obtained in huge quantities in the brewing and wine making process, as a source for the production of natural preparations with high biological value. In this study are presented results related to the recovery of beer yeast biomass from the sediments of the beer industry, by obtaining lipid extracts with valuable biochemical composition. Thus, applying the optimized autolysis method, with the use of sodium phosphate buffer at 45°C for the destruction of the cell wall and fractional extraction with hydric, alkaline and acidic solutions, various liquid and solid fractions were obtained from the biomass of brewer’s yeast with varied lipid content. So, in order to optimize the waste processing process of the beer industry and the complex recovery of yeast biomass, it is reasonable to include the lipid fraction extraction stage in the technological flow, after obtaining protein and mannoprotein extracts, which will be useful and as an additional step of purification of the β-glucan fraction.

scholarly journals Thermal denaturation in acidic solutions of double-helical ribonucleic acid from virus-like particles found in Penicillium chrysogenum. A spectrophotometric study

1971 ◽  
Vol 125 (2) ◽  
pp. 655-665 ◽  
Author(s):  
R. A. Cox ◽  
K. Kanagalingam ◽  
Elisabeth Sutherland
Keyword(s):  
Base Pair ◽  
Penicillium Chrysogenum ◽  
Sodium Phosphate ◽  
Thermal Denaturation ◽  
Spectrophotometric Study ◽  
Base Pairs ◽  
Native Form ◽  
Acidic Solutions ◽  
Ph Values ◽  
Sodium Phosphate Buffer

1. Two species of double-helical RNA isolated from mycelium of Penicillium chrysogenum were titrated with acid at 25°C and 95°C (solvent 0.1m-sodium phosphate buffer). At 25°C denaturation occurred at about pH3. At 95°C in the denatured form cytosine residues titrated as a simple monobasic acid of pK3.9 compared with pK≃2.5 for the native form at 25°C. 2. On thermal denaturation in neutral and acidic solutions one species of RNA (38% rG·rC) ‘melted’ in three distinct stages, equivalent to a mixture of three species, namely one of about 25% rG·rC, another of about 33% rG·rC and a third of about 46% rG·rC: the relative proportions were 0.25:0.35:0.40. 3. On thermal denaturation in acidic solutions the increase in the fraction of ionized cytosine residues concomitant with the ‘melting’ of rG·rC base pair also affects the spectrum especially at 280nm and serves to enhance the contribution of rG·rC base pairs at this wavelength. The increment in ε(P) at 280nm on ‘melting’ an rG·rC base pair approaches 53501·mol−1·cm−1 depending on pH, compared with 33501·mol−1·cm−1 at pH7. In contrast ε(P) at 280nm is scarcely affected by ‘melting’ rA·rU base pairs or by the protonization of adenine residues. 4. Changes in the spectrum of Escherichia coli rRNA on denaturation in acidic solutions were studied to yield the mole fractions of rA·rU and rG·rC base pairs ‘melting’ at particular pH values.

Fluorocitrate Neural Dystrophy of the Guinea Pig Cochlea

1975 ◽  
Vol 33 ◽  
pp. 368-369
Author(s):  
G.J. Spector ◽  
C.D. Carr ◽  
I. Kaufman Arenberg ◽  
R.H. Maisel
Keyword(s):  
Hearing Loss ◽  
Hair Cells ◽  
Sodium Phosphate ◽  
Sensory Cells ◽  
Barium Salt ◽  
Perfusion Medium ◽  
Cochlear Hair Cells ◽  
Neural Degeneration ◽  
Sodium Phosphate Buffer ◽  
Cochlear Neurons

All studies on primary neural degeneration in the cochlea have evaluated the end stages of degeneration or the indiscriminate destruction of both sensory cells and cochlear neurons. We have developed a model which selectively simulates the dystrophic changes denoting cochlear neural degeneration while sparing the cochlear hair cells. Such a model can be used to define more precisely the mechanism of presbycusis or the hearing loss in aging man.Twenty-two pigmented guinea pigs (200-250 gm) were perfused by the perilymphatic route as live preparations using fluorocitrate in various concentrations (15-250 ug/cc) and at different incubation times (5-150 minutes). The barium salt of DL fluorocitrate, (C6H4O7F)2Ba3, was reacted with 1.0N sulfuric acid to precipitate the barium as a sulfate. The perfusion medium was prepared, just prior to use, as follows: sodium phosphate buffer 0.2M, pH 7.4 = 9cc; fluorocitrate = 15-200 mg/cc; and sucrose = 0.2M.

Development of Carbon Emission Label for Local Ceramic Product

2014 ◽  
Vol 608 ◽  
pp. 62-67
Author(s):  
Karin Kandananond
Keyword(s):  
Carbon Footprint ◽  
Carbon Emission ◽  
Product Information ◽  
Ceramic Production ◽  
Resource Extraction ◽  
Ceramic Product ◽  
Extraction Stage ◽  
Whole Life Cycle ◽  
By Products ◽  
The Eu

Although the manufacturing businesses have played an important role in generating the highest GDP for Thailand, they also emit more greenhouse gas (GHG) than other sectors. Due to the cap and trade scheme by European Union (EU), the carbon footprint is the GHG emitted by products, organization or persons and it has to be tracked and recorded. Since the ceramic production process also has a major contribution on the emission, its carbon footprint is a piece of product information which cannot be ignored. In this research, the carbon footprint for the whole life cycle of a local ceramic product was recorded and calculated. It is interesting to note that the resource extraction stage has contributed to the highest emission followed by the product use, manufacturing, disposal and distribution. The results from this research are useful for local ceramic manufacturers who want to export their products to the EU countries and it is also important for the customers who are concerned about the environment.

Effects of sodium phosphate buffer on horseradish peroxidase thermal stability

2008 ◽  
Vol 93 (2) ◽  
pp. 569-574 ◽  
Author(s):  
L. Haifeng ◽  
L. Yuwen ◽  
C. Xiaomin ◽  
W. Zhiyong ◽  
W. Cunxin
Keyword(s):  
Thermal Stability ◽  
Horseradish Peroxidase ◽  
Phosphate Buffer ◽  
Sodium Phosphate ◽  
Sodium Phosphate Buffer

Recovery of milk fat globule membrane (MFGM) from buttermilk: effect of Ca-binding salts

2019 ◽  
Vol 86 (3) ◽  
pp. 374-376 ◽  
Author(s):  
Vitaly L. Spitsberg ◽  
Liza Ivanov ◽  
Vladimir Shritz
Keyword(s):  
Phosphate Buffer ◽  
Milk Fat ◽  
Sodium Phosphate ◽  
Milk Fat Globule Membrane ◽  
Peripheral Protein ◽  
Sodium Phosphate Buffer ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
First Time ◽  
Phosphate Groups

AbstractIn this Research Communication we present a study of the effect of Ca-binding salts on the recovery of milk fat globule membrane (MFGM) from buttermilk. Sodium phosphate buffer was used for the purpose of MFGM recovery from buttermilk for the first time and we showed that 0.1 M buffer at pH 7.2 was the most effective for the recovery of MFGM. The fact of high efficacy of sodium phosphate buffer in recovery of MFGM from buttermilk allowed us to suggest that MFGM in buttermilk is present in association with casein through Ca- bridges formed between phospholipids of MFGM and phosphate groups of casein, primarily with k-casein as the peripheral protein of casein micelles.

scholarly journals HPLC micromethod for amrinone and metabolites in patients receiving concurrent cephalosporin therapy

1996 ◽  
Vol 42 (5) ◽  
pp. 761-765
Author(s):  
J B Pappas ◽  
E M Allen ◽  
M Ross ◽  
W Banner
Keyword(s):  
Detection Limit ◽  
Mobile Phase ◽  
Sodium Phosphate ◽  
Hplc Method ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Diode Array ◽  
Supernatant Fluid ◽  
Diode Array Detector ◽  
Sodium Phosphate Buffer

Abstract Amrinone (AMR), a bipyridine derivative, is receiving increasing use in postoperative cardiac patients as an inotrope and vasodilator. The hemodynamic response to amrinone in adults is linearly related to AMR concentrations, warranting therapeutic drug monitoring. We report a rapid microsample HPLC method for monitoring AMR and its principal metabolites, N-acetyl (N-ac) and N-glycolyl (N-gly) AMR. Serum was precipitated with acetonitrile, and the supernatant fluid was then injected into a C18 narrow-bore column. The mobile phase consisted of a 0.1 mol/L sodium phosphate buffer (pH 6) with a gradient of acetonitrile going from 50 to 100 mL/L of eluent. Detection with a diode-array detector (DAD) concurrently monitored the absorbances at 320 and 345 nm. Monitoring 320 nm allows optimal quantification of AMR, N-gly, and N-ac. Patients often receive concurrent cephalosporin therapy, which is detectable at 320 nm but not 345 nm. Because cephalosporins coelute with AMR or metabolites, monitoring at 345 nm allows separation of these antibiotics from AMR and metabolites while retaining a detection limit of 0.5 mg/L.

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