Determination of Favipiravir from Pharmaceutical Dosage Form by Extractive Ion Pair Complex Colorimetric Method

Pharmaceutical Dosage Forms

Simple sensitive and accurate extractive colorimetric method was developed for the estimation of favipiravir in Pharmaceutical dosage forms. The method was based on the formation of colored ion pair complexes by the drugs with thiocynate ions. These ion pair complexes were quantitatively extracted under the experimental condition in chloroform. The absorbance values were measured at 618 respectively. The proposed method was validated statistically. A recovery of method was carried out by standard addition methods. The Beer’s law ranges were found to be 1-12μg/ml, respectively. The low values of standard deviation and percentage RSD indicate high precision of method. Hence the method is useful for routine estimation of favipiravir in tablets respectively.

Spectrophotometric analysis of Azithromycin and its pharmaceutical dosage forms: Comparison between Spectrophotometry and HPLC

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.21981 ◽

2015 ◽

Vol 12 (2) ◽

pp. 171-179 ◽

Cited By ~ 3

Author(s):

Nahid Sharmin ◽

Nazia Sultana Shanta ◽

Sitesh C Bachar

Keyword(s):

Statistical Analysis ◽

Spectrophotometric Method ◽

Dosage Form ◽

Dosage Forms ◽

Spectrophotometric Analysis ◽

Ich Guidelines ◽

Good Accordance

A simple, reliable, precise and sensitive UV-spectrophotometric method was developed and validated for the estimation of azithromycin in pharmaceutical dosage form and compared with official USP 2010 method. The proposed method utilizes the oxidation of azithromycin with potassium permanganate to liberate formaldehyde. This formaldehyde reacts with acetone-ammonium reagent and produces yellow colored chromogen 3,5-diacetyl-2,6-dihydrolutidine. The colored solution exhibited a maximum absorption at 412 nm which can be detected with UVspectrophotometer. The method was found linear over the concentration range 80% to 120% of the working concentration (R2=0.999). The intra- and inter-day RSD (n = 6) was ? 2.0%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The proposed method was successfully applied for determination of azithromycin and the results have been compared with HPLC and thus enabling the utility of this new method for routine analysis azithromycin in pharmaceutical dosage forms DOI: http://dx.doi.org/10.3329/dujps.v12i2.21981 Dhaka Univ. J. Pharm. Sci. 12(2): 171-179, 2013 (December)

Spectrophotometric Determination of Zidovudine in Pharmaceutical Dosage Forms

E-Journal of Chemistry ◽

10.1155/2012/757916 ◽

2012 ◽

Vol 9 (1) ◽

pp. 89-92

Author(s):

J. Sudhakar Reddy ◽

MD. S. Maqsood Ahmed ◽

I. E. Chakravarthy ◽

K. Prabhavathi

Keyword(s):

Statistical Analysis ◽

Dosage Form ◽

Dosage Forms ◽

Ion Pair ◽

Reagent Blank ◽

Extractable Complex ◽

Bulk Drug ◽

Tablet Dosage

A simple, sensitive and economical spectrophotometric method has been developed for the determination of zidovudine in commercial dosage forms. The method was based on the formation of chloroform extractable complex of zidovudine with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of zidovudine in bulk drug and in its tablet dosage form.

Spectrophotometric quantification of dolutegravir based on redox reaction with Fe3+/1,10-phenanthroline

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-020-00121-2 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Swathi Naraparaju ◽

Durai Ananda Kumar Thirumoorthy ◽

Sunitha Gurrala ◽

Asra Jabeen ◽

Pani Kumar D. Anumolu

Keyword(s):

Redox Reaction ◽

Quantitative Measurement ◽

Colorimetric Method ◽

Dosage Forms ◽

Control Analysis ◽

Colored Complex ◽

Quality Control Analysis

Abstract Background A simple and sensitive spectrophotometric method was developed for the quantitative measurement of dolutegravir in pure form and pharmaceutical formulation. The present method was based on redox reaction between dolutegravir and ferric chloride, which upon complexation with 1,10-phenanthroline formed an orange-colored complex that showed absorption maximum at 520.0 nm. Results The developed method obeyed linearity in the concentration range of 40.00–140.00 μg/mL. The method was also validated as per International Council for Harmonization guidelines and the results were within acceptance values. The validated method was employed for the determination of dolutegravir in pharmaceutical dosage form and the percentage assay value was found to be 102.5, which is in agreement with its label claimed. Conclusion The developed redox-based colorimetric method could be used in the routine quality control analysis of dolutegravir present in various pharmaceutical dosage forms.

Determination of Bismuth in Pharmaceutical Dosage Forms by Conventional DC Polarography

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/55.1.155 ◽

1972 ◽

Vol 55 (1) ◽

pp. 155-160

Author(s):

William M Plank

Keyword(s):

Standard Deviation ◽

Relative Standard Deviation ◽

Collaborative Study ◽

Colorimetric Method ◽

Dosage Forms ◽

Official Method ◽

Relative Standard ◽

Dc Polarography

Abstract A method has been investigated for the determination of bismuth in pharmaceutical dosage forms by conventional dc polarography. The method is applicable to tablets, emulsions, suspensions, injectables, and bulk powders. A collaborative study has shown the method to be superior in accuracy and precision to the present official colorimetric method, 36.300- 36.304. The results from 16 determinations performed by 8 collaborators were: 100.0±1.5% recovery on a synthetic powder mix, a relative standard deviation of 1.9% on a commercial tablet sample, and a relative standard deviation of 2.1% on a magma sample. By comparison, the collaborative data reported for the present official method gave relative standard deviations of 3.4 and 3.2% on 2 samples of powder mixtures. It is recommended that the polarographic method be adopted as official first action.

Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography

E-Journal of Chemistry ◽

10.1155/2009/795896 ◽

2009 ◽

Vol 6 (s1) ◽

pp. S59-S64 ◽

Cited By ~ 3

Author(s):

Prafulla Kumar Sahu ◽

M. Mathrusri Annapurna

Keyword(s):

Mobile Phase ◽

High Performance ◽

Dosage Form ◽

Internal Standard ◽

Dosage Forms ◽

Reverse Phase ◽

Rp Hplc

RP-HPLC analytical method for the estimation of nabumetone in pharmaceutical dosage forms was developed and validated. A Hypersil ODS C18, 4.6 mm x 250 mm, 5 μm column from Supelco (India), with mobile phase comprised of acetonitrile: triple distilled water (50:50) with a total run time of 18 min was used and the wavelength of the detector was set at 230 nm. Stavudin is used as internal standard. The retention times were 14.167 min and 1.967 min for nabumetone and stavudin (IS) respectively. The extraction recovery of nabumetone from pharmaceutical dosage form (tablets) was >101% and the calibration curve was linear (r2= 0.995) over nabumetone concentrations ranging from 1 to 200 µg/mL. The method had an accuracy of >99% and LOD and LOQ of 0.17482 µg/mL and 0.5827 µg/mL respectively. The method reported is simple, reliable, precise and accurate and has the capability of being used for determination of nabumetone in bulk and pharmaceutical dosage forms.

Development and validation of new analytical method for the estimation of fluoxetine in bulk and dosage form by UV spectrophotometry

International Journal of Research In Pharmaceutical Chemistry and Analysis ◽

10.33974/ijrpca.v1i2.54 ◽

2019 ◽

Vol 1 (2) ◽

pp. 36-39

Author(s):

Uday sankar raju K ◽

Vimalakkannan T ◽

K. Ravindra Reddy ◽

P. Naveena ◽

R. Riharika raj ◽

...

Keyword(s):

Standard Deviation ◽

Quantitative Determination ◽

Dosage Form ◽

Dosage Forms ◽

Uv Spectrophotometry ◽

Precise Method ◽

Absorbance Measurement ◽

Development And Validation

A simple, rapid and precise method is developed for the quantitative determination of Fluoxetine in combined pharmaceutical-dosage forms. The method was based on UV Spectrophotometric determination of Fluoxetine drug using Beer-Lamberts Law. It involves absorbance measurement at 224 nm (λmax of Fluoxetine) in water. For UV Spectrophotometric method, linearity was obtained in concentration range of 5-30 mcg/ml with regression 0.999 for Fluoxetine respectively. Recovery was in the range of 98 -102%; the value of standard deviation and %R.S.D was found to be < 2 shows high precision of the method..

Development and validation of new analytical method for the simultaneous estimation of omeprazole and domperidone in pharmaceutical dosage form by UV spectrophotometry

International Journal of Research In Pharmaceutical Chemistry and Analysis ◽

10.33974/ijrpca.v1i2.63 ◽

2019 ◽

Vol 1 (2) ◽

pp. 44-46

Author(s):

V. Pavan Kumar ◽

C. Bhanu Chandra ◽

N. Devendra ◽

M. Kishor Kumar ◽

S. Reddy Basha ◽

...

Keyword(s):

Dosage Form ◽

Simultaneous Equation ◽

Dosage Forms ◽

Simultaneous Estimation ◽

Uv Spectrophotometry ◽

Ich Guidelines ◽

Development And Validation

A simple, rapid and precise method was developed for the quantitative simultaneous determination of Omeprazole and Domperidone in combined pharmaceutical-dosage forms. The method was based on UV-Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 291 nm (λmax of Omeprazole) and 289 nm (λmax of Domperidone) in Methanol: Acetonitrile (30:70 v/v). For UV Spectrophotometric method, linearity was obtained in concentration range of 1-15 µg/ml for Domperidone and 1-50 µg/ml for Omeprazole respectively, with regression 0.999 and 0.999 for Domperidone and Omeprazole respectively. Recovery was in the range of 99 -103%; the value of standard deviation and %R.S.D were found to be < 2 %; shows the high precision of the method., in accordance with ICH guidelines. The method has been successively applied to pharmaceutical formulation and was validated according to ICH guidelines.

Spectrophotometric Determination of Alendronate Sodium by using Sodium-1,2-Naphthoquinone-4-Sulphonate

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.4.7 ◽

2012 ◽

Vol 4 (4) ◽

pp. 1563-1568

Author(s):

Sagar Suman Panda ◽

Ravi Kumar B V V ◽

D Patanaik

Keyword(s):

Spectrophotometric Method ◽

Absorption Maximum ◽

Dosage Form ◽

Dosage Forms ◽

Alendronate Sodium ◽

Colored Complex ◽

Recovery Study ◽

Assay Conditions

A simple, precise and accurate spectrophotometric method was developed for analysis of the osteoporesis drug alendronate sodium (ALS). The method is based on reaction of the drug with sodium-1,2-naphthoquinone-4-sulphonate (NQS) in presence of alkali to form a brown colored complex giving absorption maximum at 525 nm. The drug obeyed Beer’s law in the range of 5-70 µg/ml with a correlation coefficient of 0.999. The LOD and LOQ values are 1.7 µg/ml and 5.0 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 99.37%-100.46%. The R.S.D. values for intraday and inter-day precision were found to be 0.48 and 0.62, respectively. The optimized assay conditions were applied successfully for determination of ALS in pharmaceutical dosage forms. No interference was observed from the excipients present in the dosage form. The method is statistically validated as per the ICH requirements.

An Overview of Excipients Classification and Their Use in Pharmaceuticals

Current Pharmaceutical Analysis ◽

10.2174/1573412916999200605163125 ◽

2020 ◽

Vol 16 ◽

Author(s):

Cansel Kose Ozkan ◽

Ozgur Esim ◽

Ayhan Savaser ◽

Yalcin Ozkan

Keyword(s):

Drug Delivery ◽

Drug Delivery Systems ◽

Dosage Form ◽

Dosage Forms ◽

Design Development ◽

Product Identification ◽

Direct Administration ◽

Active Substances

: The content and the application of pharmaceutical dosage forms must meet several basic requirements to ensure and maintain efficiency, safety and quality. A large number of active substances have limited ability to direct administration. Excipients are generally used to overcome the limitation of direct administration of these active substances. However, the function, behavior and composition of the excipients need to be well known in the design, development and production of pharmaceutical dosage forms. In this review, excipients used to assist in any pharmaceutical dosage form production processes of drugs, to preserve, promote or increase stability, bioavailability and patient compliance, to assist in product identification / separation, or to enhance overall safety and effectiveness of the drug delivery system during storage or use are explained. Moreover, the use of these excipients in drug delivery systems are identified. Excipient toxicity, which is an issue discussed in the light of current studies, also discussed in this review.

Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

Open Chemistry ◽

10.1515/chem-2016-0024 ◽

2016 ◽

Vol 14 (1) ◽

pp. 258-266 ◽

Cited By ~ 16

Author(s):

Sayed M Derayea ◽

Mahmoud A Omar ◽

Mohamed Aboel-Kasem Abdel-Lateef ◽

Ahmed I. Hassan

Keyword(s):

Fluorescence Quenching ◽

Beta Blockers ◽

Dosage Forms ◽

Ion Pair ◽

Content Uniformity ◽

Eosin Y ◽

Quenching Effect ◽

Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.