scholarly journals ESSENTIAL OILS AND TOTAL PHENOLIC CONTENT CORRELATED WITH ANATOMICAL FEATURES IN LAVANDULA ANGUSTIFOLIA MILL. ORGANS

Author(s):  
Dan Răzvan POPOVICIU
Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2489
Author(s):  
Cristina Laura Popa ◽  
Andreea Lupitu ◽  
Maria Daniela Mot ◽  
Lucian Copolovici ◽  
Cristian Moisa ◽  
...  

Many plants belonging to the Lamiaceae family are rich in essential oils (EOs) which are intensively used for aromatherapy, food and beverage flavoring, alternative medicine, cosmetics, and perfumery. Aerial parts of Thymus vulgaris L., Thymus pannonicus All., Lavandula angustifolia L., Lavandula x intermedia, Origanum vulgare L., and Origanum vulgare var. aureum L. were subjected to hydrodistillation, and both resulting fractions were analyzed. The purpose of this study was to determine the chemical composition, antioxidant activity, and total phenolic content of six essential oils and their corresponding hydrolats (HDs) through GC-MS and spectrophotometric analyses. Overall, 161 compounds were identified, some found exclusively in essential oils and others in hydrolats, making them individual products with specific end purposes. The total phenolic content was the highest for the Thymus vulgaris L. EOs (3022 ± mg GAE L−1), because of its high phenolic oxygenated monoterpenes content (thymol and carvacrol) and the smallest for the Lavandula angustifolia L. EOs (258.31 ± 44.29 mg GAE L−1), while hydrolats varied from 183.85 ± 0.22 mg GAE L−1 for Thymus vulgaris L. HD and 7.73 mg GAE L−1 for Thymus pannonicus All. HD. Significant antioxidant effects determined through DPPH• and ABTS•+ assays were also observed in samples with higher hydrophilic compounds. The highest antioxidant activity was determined for Thymus vulgaris L. EO and its corresponding HD. Although EOs are the principal traded economic product, HDs represent a valuable by-product that could still present intense antiseptic activities, similar to their corresponding EOs (thyme and oregano), or have multiple aromatherapy, cosmetics, and household applications (lavender and lavandin).


Background and Aims: Herbal essential oils (EOs) have antimicrobial and antioxidant activities due to the high amount of bioactive compounds; therefore, they are considered good candidates for applications in the food and pharmaceutical industries. The present study aimed to assess the total phenolic content and in vitro comparative study of the biological activities of EOs from different plants (e.g., clove, common sage, savory, and organum). Materials and Methods: In this experimental study, total phenolic content in EOs was determined, and their antioxidant capacity was measured by the DPPH free radical scavenging method. The components of essential oil were identified using a Gas chromatography-mass spectrometry (GC-MS) device. Moreover, the antibacterial activity of EOs was evaluated by the disk diffusion method, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated by the broth macro dilution method. Data were analyzed using one-way analysis of variance (ANOVA) and Duncan's multiple range test. Results: The highest content of total phenol (157.07±2.37 mg GAL/g dry weight) was recorded for EO of organum. Moreover, the highest percentage of free radical scavenging was determined at 98.142% for organum. The results of GC/MS analysis depicted that monoterpenes were the main compounds of Eos of organum, and the highest value was obtained for the alpha-pinene (74.04%). Furthermore, the results of antibacterial activity of EOs demonstrated that the highest zone of inhibition with a diameter of 44±0.81 mm was observed for the EO of organum. The lowest values of MIC and MBC were reported as 0.275 and 0.55 mg/mL for the EOs of organum and savory against gram-positive Bacillus cereus, respectively. Conclusion: As evidenced by the results of the current study, the assessed Eos, specifically those of organum, have effective antioxidant and antibacterial activity against bacteria strains, especially gram-positive ones, and can be used as safe antimicrobial compounds in food and health products.


Author(s):  
Cristian Moisa ◽  
Lucian Copolovici ◽  
Georgeta Pop ◽  
Andreea Lupitu ◽  
Virgiliu Ciutina ◽  
...  

Abstract Origanum vulgare L. var. aureum is an aromatic medicinal plant belonging to the Lamiaceae family, rich in essential oils and antioxidants. Its specific chemical composition represents an important source of biologically active principles with diverse applications in food products and nutraceuticals. The aim of this study was to determine the differences between chemical composition of volatile oils, phenolic content and antioxidant activity of different plants parts: leaves, flowers, and stems. Essential oils from leaves and flowers had similar compositions with only few differences between γ-terpinene and trans-ß-ocimene. Total phenolic content and antioxidant activity were determined for the hydro-alcoholic extract obtained from post-distillation oregano waste material. The total phenolic content was determined using Folin-Ciocalteu reagent and varied from 3173 to 6522 mg GAE/L. The major compounds identified by HPLC were syringic acid, pyrocatechol, and catechin. Furthermore, all extracts showed high antioxidant activity, ranging from 31.3 to 44.5 mg GAE/L, with an inhibition percent varying from 63.1 to 88.6%.


2020 ◽  
Vol 30 (6) ◽  
pp. 770-772
Author(s):  
Hanah T. Rheay ◽  
Kevin Lombard ◽  
Catherine Brewer ◽  
F. Omar Holguin

Neomexicanus hops (Humulus lupulus var. neomexicanus) are receiving increased attention within the craft beer and nutraceutical industries. Characterization of bittering acids and essential oils in two neomexicanus varieties revealed wide ranges of bittering acid compositions and distinct essential oil profiles compared with ‘Cascade’ common hops (H. lupulus). Total phenolic content (TPC), expressed as gallic acid equivalent (GAE), in neomexicanus hops ranged from 50 to 100 mg·g−1 GAE, consistently higher than published literature values for hop TPC (2 to 50 mg·g−1 GAE). Results indicate that, compared with ‘Cascade’, neomexicanus hops have unique phytochemical characteristics, which may lead to new applications in brewing and nutraceutical fields.


2019 ◽  
Vol 70 (5) ◽  
pp. 1800-1804
Author(s):  
Gabriela Stanciu ◽  
Florin Aonofriesei ◽  
Simona Lupsor ◽  
Antoanela Popescu ◽  
Rodica Sirbu

Studies of total phenolic content and identification and quantification of phenolic compounds by HPLC-DAD from hydro-alcoholic and alcoholic macerates of Lavandula angustifolia L flowers from Dobrogea area are reported. The total phenolic content and the phenolic profile have been measured using Folin-Ciocalteau method, respectively adapted USP30 HPLC method in macerates of Lavandula angustifolia L flowers after 5 days of contact with either methanol (M1) or methanol: water 1 : 1 (V : V) mixture (M2). The values of TPC show that macerate M2 is more rich in phenolic compounds (3520 mg/100g d.w.), than in the case of macerate M1 (2860 mg/100 g d.w.). The obtained data of phenolic compounds determined by HPLC-DAD method were compared with the available authentic standards. Four individual phenolic compounds were found in macerate M1 and six individual phenolic compounds in macerate M2. Among gallic acid and chlorogenic acid, as major phenolic compounds, high concentrations of ellagic acid in M2 (514.249 mg/100 g d.w.) and M1 (499.487 mg/100g d.w.) were found. The antimicrobial activity of tested macerates indicates that Lavandula sp. has a moderate effect on the bacterial and Candida growth.


2019 ◽  
Vol 8 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Adel F. Ahmed ◽  
Fatma A.K. Attia ◽  
Zhenhua Liu ◽  
Changqin Li ◽  
Jinfeng Wei ◽  
...  

2010 ◽  
Vol 5 (7) ◽  
pp. 492-498 ◽  
Author(s):  
Hamid Reza Monsef-Esf ◽  
Abdolhossein Miri ◽  
Mohsen Amini ◽  
Yaghoub Amanzadeh ◽  
Abbas Hadjiakhoo ◽  
...  

Author(s):  
JEYAKANI M ◽  
RAJALAKSHMI M

Objectives: Essential oil and their compounds are getting increasing interest due to their multipurpose functional as alternatives to artificial preservatives. The aim of this study was focused to comparative analyses on hydrodistillation, total phenolic content (TPC), and antioxidant activity of essential oil derived from Melaleuca alternifolia (M. alternifolia) leaves procured from natural leaves, in-vitro leaves, and callus sources. Methods: The essential oil was extracted using hydrodistillation. The Folin-Ciocalteu method was used to determine the TPC equivalent and antioxidant activity of essential oils. Results: TPC of essential oil from in-vitro sources of leaves contained 14.79 mg gallic acid equivalent/g dry plant material and its IC50 value was found to be 70% in 1-diphenyl 2-picrylhyorazyl assay. Thus, this source of essential oil showed good free radical scavenging activity. Conclusion: The estimated biological potential was obtained in the essential oil from in-vitro sources of leaves M. alternifolia.


Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6853
Author(s):  
Ahmed Mohamed Hamed ◽  
Awad A. Awad ◽  
Ahmed E. Abdel-Mobdy ◽  
Abdulhakeem Alzahrani ◽  
Ahmad Mohammad Salamatullah

Eucalyptus (Eucalyptus camaldulensis) and Myrrh (Commiphora Myrrha) essential oils (EOs) stand out for their benefits in terms of health and functionality. Buffalo set yogurt enriched with different concentrations of EOs (0.3, 0.6, and 0.9%) were investigated. The effects of addition on sensory, syneresis, antibacterial activity, and bioactive properties (total phenol content and antioxidant activity) of yogurt were studied. The most acceptable organoleptic properties of treated yogurt were those samples treated with Eucalyptus oil. The levels of syneresis were decreased by increasing the concentration of EOs. Moreover, the antioxidant activity, antibacterial activity, and total phenolic content were enhanced by increasing the concentration of EOs. Yogurt with 0.9% Eucalyptus oil showed the highest antioxidant activity and total phenolic content. The same concentration of Eucalyptus oil showed the highest antibacterial activity against S. typhimurium (the inhibition zone was 20.63 mm) then E. coli (the inhibition zone was 19.43 mm). On the other hand, the highest antibacterial effect against L. monocytogene was for Myrrh oil-enriched yogurt by 0.9% and the inhibition zone was 19.21 mm. The obtained results showed that Eucalyptus and Myrrh oils can be applied to yogurt to improve its beneficial properties in terms of physical characteristics and for human health due to their antioxidant activity and phenolic materials.


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