scholarly journals Clinicopathologic Significance of Histologic Grade, Pgp, and P53 Expression in Canine Lymphoma

2013 ◽  
Vol 49 (3) ◽  
pp. 175-184 ◽  
Author(s):  
Ravinder S. Dhaliwal ◽  
Barbara E. Kitchell ◽  
EJ Ehrhart ◽  
Victor E. Valli ◽  
Nikolaos G. Dervisis
Keyword(s):  
Histologic Grade ◽  
Canine Lymphoma ◽  
P53 Expression ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Histologic Evaluation ◽  
Chemotherapy Protocol ◽  
First Remission ◽  
Resistance Markers ◽  
Significant Expression

To characterize the expression of P-glycoprotein (Pgp) and p53 in different histologic grades of canine multicentric lymphosarcoma (LSA), 31 cases of LSA without prior treatment were studied. The expression levels of the Pgp and p53 proteins were evaluated for their clinicopathologic significance among standard histologic evaluation. Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded archival samples of 31 previously untreated LSA cases to detect the expression of Pgp and p53. All dogs were subsequently treated with a combination chemotherapy protocol. Remission and survival durations were evaluated for correlation with histologic grade and presence of drug resistance markers. Of the 31 cases, 24 (80%) and 7 (22%) were positive for Pgp and p53, respectively. Overall, the median survival and duration of remission in the study was 246 days and 137 days, respectively. The National Cancer Institute working formulation histologic grade was not associated with either survival or duration of first remission (DOR). The Pgp protein expression and DOR and survival was not statistically significant. Expression of p53 was statistically correlated with survival.

scholarly journals p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry.

1992 ◽  
Vol 40 (7) ◽  
pp. 1047-1051 ◽  
Author(s):  
B J Kerns ◽  
P A Jordan ◽  
M B Moore ◽  
P A Humphrey ◽  
A Berchuck ◽  
...  
Keyword(s):  
Breast Cancers ◽  
Fresh Tissue ◽  
P53 Overexpression ◽  
P53 Expression ◽  
Ovarian Cancers ◽  
Formalin Fixed Paraffin ◽  
Wide Range ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Formalin Fixed

Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.

scholarly journals Parvovirus-induced encephalitis in a juvenile raccoon

2020 ◽  
Vol 33 (1) ◽  
pp. 140-143
Author(s):  
Arno Wünschmann ◽  
Robert Lopez-Astacio ◽  
Aníbal G. Armién ◽  
Leslie Reed ◽  
Colin R. Parrish
Keyword(s):  
Cerebral Cortex ◽  
Cell Loss ◽  
Canine Parvovirus ◽  
Vp2 Gene ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Histologic Evaluation ◽  
Ns1 Gene ◽  
Gross Lesions ◽  
Pcr Targeting

A juvenile raccoon was euthanized because of severe neurologic signs. At postmortem examination, no significant gross lesions were present. Histologic evaluation demonstrated nonsuppurative encephalitis in thalamus, brainstem, and hippocampus, cerebellar Purkinje cell loss, as well as poliomyelitis and demyelination of the spinal cord. Parvovirus antigen–specific immunohistochemistry revealed immunopositive neurons in the brainstem, cerebral cortex, and hippocampus. A few Purkinje cells were also immunopositive. DNA extracted from formalin-fixed, paraffin-embedded brain tissue (thalamus, hippocampus, cerebral cortex) yielded a positive signal using PCR targeting both feline and canine parvovirus. Sequencing analyses from a fragment of the NS1 gene and a portion of the VP2 gene confirmed the presence of DNA of a recent canine parvovirus variant (CPV-2a–like virus) in the cerebellum. Our case provides evidence that a recent canine parvovirus (CPV) strain ( Carnivore protoparvovirus 1) can infect cerebral and diencephalic neurons and cause encephalitis in an otherwise healthy raccoon. Parvovirus-induced encephalitis is a differential diagnosis of rabies and canine distemper in raccoons with neurologic signs.

scholarly journals Study Correlation between Some Immune Markers in Patients with Lichen Planus: Review Article

2021 ◽  
Vol 8 (2) ◽  
pp. 38-44
Author(s):  
Nagham H. Ali ◽  
Amir A. Majeed
Keyword(s):  
Lichen Planus ◽  
Gene Mutations ◽  
P53 Gene ◽  
Autoimmune Disorder ◽  
Immune Markers ◽  
P53 Expression ◽  
Formalin Fixed Paraffin ◽  
A Genome ◽  
P53 Immunohistochemistry ◽  
Formalin Fixed Paraffin Embedded

Lichen planus is an autoimmune disorder that primarily affects the body's skin and multiple mucous membranes. In multicellular species, protein 53 and syndecan-1protein play an significant role since they control the cell cycle and thus play a major role in tumor suppression and cancer prevention. The p53 gene was therefore identified as a "genome protector," referring to its role in preserving the integrity of genetic information by preventing gene mutations. The purpose of this analysis was to study the association between certain immune markers in lichen planus patients. The aim of this study: Study correlation between some immune markers in patients with lichen planus. Methods: 'Thirty formalin-fixed, paraffin-embedded tissue pieces, diagnosed as Oral Lichen planus (OLP), were included in the sample of this report. Results: Evaluation of P53 Immunohistochemistry and Evaluation of syndecan-1protein Immunohistochemistry Conclusion: The marker p53 expression is high in the patient with Lichen Planus while the second studed markersyndecan-1 protein is less than marker p53.

scholarly journals Expression of “Connexin 43” in Colorectal Carcinomas: Histopathological and Immunohistochemical Study

2020 ◽  
Vol 8 (A) ◽  
pp. 354-359
Author(s):  
Essam Ayad ◽  
Ahmed Naeem Eesa ◽  
Rabab Radi ◽  
Lubna Omer El Farouk Abdel-Salam
Keyword(s):  
Connexin 43 ◽  
Cancer Chemoprevention ◽  
Colorectal Carcinogenesis ◽  
Formalin Fixed Paraffin ◽  
Gap Junction Intercellular Communication ◽  
Formalin Fixed Paraffin Embedded ◽  
Histologic Evaluation ◽  
Cell Growth And Differentiation ◽  
Hematoxylin And Eosin ◽  
Cx 43

BACKGROUND: Colorectal cancer is one of the most common cancers worldwide and leading cause of cancer related deaths. Connexins are integral membrane proteins that form channels between adjacent cells. Gap junction intercellular communication plays essential roles in tissue homoeostasis and regulation of cell growth and differentiation. Connexins can act as either tumor suppressors or tumor promoters. The human connexin protein family contains 21 members, of which the most widely studied is connexin 43 (Cx 43). OBJECTIVES: Investigation of immunohistochemical expression of Cx 43 in cases of colorectal adenoma and carcinoma and correlation of this expression with the clinico-pathological aspects of the tumors. MATERIALS AND METHODS: Seventy formalin fixed paraffin embedded BC tissue sections were randomly collected. All the available data were collected from the patients’ reports. The paraffin blocks were sectioned and stained with hematoxylin and eosin stains for histologic evaluation. Additional sections were immunostained with Cx 43. RESULTS: Cx 43 expression was negative in all studied cases. CONCLUSION: Cx 43 is a tumor suppressor that is lost early in colorectal carcinogenesis and can be considered as potential target for cancer chemoprevention and chemotherapy aiming at restoration of normal connexin expression and functional gap junctions.

Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq

2020 ◽  
pp. 37-40
Keyword(s):  
Nucleotide Sequence ◽  
Phylogenetic Tree ◽  
Evolutionary Relationship ◽  
Nucleotide Sequencing ◽  
Sequencing Analysis ◽  
Breast Cancer Patients ◽  
The North ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
History Of

Genetic variety examination has demonstrated fundamental to the understanding of the epidemiological and developmental history of Papillomavirus (HPV), for the development of accurate diagnostic tests and for efficient vaccine design. The HPV nucleotide diversity has been investigated widely among high-risk HPV types. To make the nucleotide sequence of HPV and do the virus database in Thi-Qar province, and compare sequences of our isolates with previously described isolates from around the world and then draw its phylogenetic tree, this study done. A total of 6 breast formalin-fixed paraffin-embedded (FFPE) of the female patients were included in the study, divided as 4 FFPE malignant tumor and 2 FFPE of benign tumor. The PCR technique was implemented to detect the presence of HPV in breast tissue, and the real-time PCR used to determinant HPV genotypes, then determined a complete nucleotide sequence of HPV of L1 capsid gene, and draw its phylogenetic tree. The nucleotide sequencing finding detects a number of substitution mutation (SNPs) in (L1) gene, which have not been designated before, were identified once in this study population, and revealed that the HPV16 strains have the evolutionary relationship with the South African race, while, the HPV33 and HPV6 showing the evolutionary association with the North American and East Asian race, respectively.

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