scholarly journals Study Correlation between Some Immune Markers in Patients with Lichen Planus: Review Article

2021 ◽  
Vol 8 (2) ◽  
pp. 38-44
Author(s):  
Nagham H. Ali ◽  
Amir A. Majeed
Keyword(s):  
Lichen Planus ◽  
Gene Mutations ◽  
P53 Gene ◽  
Autoimmune Disorder ◽  
Immune Markers ◽  
P53 Expression ◽  
Formalin Fixed Paraffin ◽  
A Genome ◽  
P53 Immunohistochemistry ◽  
Formalin Fixed Paraffin Embedded

Lichen planus is an autoimmune disorder that primarily affects the body's skin and multiple mucous membranes. In multicellular species, protein 53 and syndecan-1protein play an significant role since they control the cell cycle and thus play a major role in tumor suppression and cancer prevention. The p53 gene was therefore identified as a "genome protector," referring to its role in preserving the integrity of genetic information by preventing gene mutations. The purpose of this analysis was to study the association between certain immune markers in lichen planus patients. The aim of this study: Study correlation between some immune markers in patients with lichen planus. Methods: 'Thirty formalin-fixed, paraffin-embedded tissue pieces, diagnosed as Oral Lichen planus (OLP), were included in the sample of this report. Results: Evaluation of P53 Immunohistochemistry and Evaluation of syndecan-1protein Immunohistochemistry Conclusion: The marker p53 expression is high in the patient with Lichen Planus while the second studed markersyndecan-1 protein is less than marker p53.

scholarly journals Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Satoshi Tamura ◽  
Satoshi Osawa ◽  
Natsuki Ishida ◽  
Takahiro Miyazu ◽  
Shinya Tani ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Gene Mutations ◽  
Pcr Amplification ◽  
Kinase Domain ◽  
Resistance Mutations ◽  
Cmv Infection ◽  
Nested Polymerase Chain Reaction ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Ul97 Gene

AbstractCytomegalovirus (CMV) reactivation in the colon is common in patients with severe ulcerative colitis (UC). Ganciclovir (GCV) resistance conferring CMV UL97 gene mutations have been reported in recent years. However, the prevalence of UL97 gene mutations in GCV-naive CMV infection in the colon remains unknown. We investigated the prevalence of CMV UL97 gene mutations in patients with colonic CMV infection associated with or without UC. Twenty-two GCV-naive patients with colonic CMV infection, 15 with UC and 7 with other diseases, were enrolled. Frozen biopsy samples or formalin-fixed paraffin-embedded samples were used for nested polymerase chain reaction (PCR) amplification of the UL97 gene. Sanger DNA sequencing was performed. In comparison with AD169 reference strain, natural polymorphisms were frequently detected in codons N68D (100%), I244V (100%), and D605E (86.4%). Seven polymorphisms were detected infrequently (< 10%) outside the kinase domain. However, no known GCV resistance mutations were found. There seemed to be no difference between the ratio of polymorphisms in patients with and without UC. In conclusions, we did not detect UL97 gene mutations associated with GCV resistance in GCV-naive patients with or without UC. Consistent with previous reports, D605E polymorphism may be used as a genetic marker for CMV in East Asian countries.

KIT Gene Mutations and Patterns of Protein Expression in Mucosal and Acral Melanoma

2012 ◽  
Vol 16 (2) ◽  
pp. 135-142 ◽  
Author(s):  
Suzan Abu-Abed ◽  
Nancy Pennell ◽  
Teresa Petrella ◽  
Frances Wright ◽  
Arun Seth ◽  
...  
Keyword(s):  
Protein Expression ◽  
Kinase Inhibitors ◽  
Case Series ◽  
Gene Mutations ◽  
Mucosal Melanoma ◽  
Acral Melanoma ◽  
Formalin Fixed Paraffin ◽  
Kit Gene ◽  
Formalin Fixed Paraffin Embedded ◽  
Optimized Conditions

Background: Recently characterized KIT (CD117) gene mutations have revealed new pathways involved in melanoma pathogenesis. In particular, certain subtypes harbor mutations similar to those observed in gastrointestinal stromal tumors, which are sensitive to treatment with tyrosine kinase inhibitors. Objective: The purpose of this study was to characterize KIT gene mutations and patterns of protein expression in mucosal and acral melanoma. Methods: Formalin-fixed, paraffin-embedded tissues were retrieved from our archives. Histologic assessment included routine hematoxylin-eosin stains and immunohistochemical staining for KIT. Genomic DNA was used for polymerase chain reaction-based amplification of exons 11 and 13. Results: We identified 59 acral and mucosal melanoma cases, of which 78% showed variable levels of KIT expression. Sequencing of exons 11 and 13 was completed on all cases, and 4 (6.8%) mutant cases were isolated. Conclusion: We successfully optimized conditions for the detection of KIT mutations and showed that 8.6% of mucosal and 4.2% of acral melanoma cases at our institution harbor KIT mutations; all mutant cases showed strong, diffuse KIT protein expression. Our case series represents the first Canadian study to characterize KIT gene mutations and patterns of protein expression in acral and mucosal melanoma.

p53 gene mutations in soft-tissue sarcomas - correlations with p53 immunohistochemistry and DNA ploidy

1997 ◽  
Vol 123 (4) ◽  
pp. 211-218 ◽  
Author(s):  
Regine Schneider-Stock ◽  
Kathrin Radig ◽  
Yoshinao Oda ◽  
Walter Mellin ◽  
Janusz Rys ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Dna Ploidy ◽  
Gene Mutations ◽  
Soft Tissue Sarcomas ◽  
P53 Gene ◽  
P53 Immunohistochemistry ◽  
P53 Gene Mutations

p53 gene mutations in soft-tissue sarcomas-correlations with p53 immunohistochemistry and DNA ploidy

1997 ◽  
Vol 123 (4) ◽  
pp. 211-218 ◽  
Author(s):  
Regine Schneider-Stock ◽  
Kathrin Radig ◽  
Yoshinao Oda ◽  
Walter Mellin ◽  
Janusz Rys ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Dna Ploidy ◽  
Gene Mutations ◽  
Soft Tissue Sarcomas ◽  
P53 Gene ◽  
P53 Immunohistochemistry ◽  
P53 Gene Mutations

scholarly journals Targeted Mutational Analysis of Cortisol-Producing Adenomas

2021 ◽  
Author(s):  
Juilee Rege ◽  
Jessie Hoxie ◽  
Chia-Jen Liu ◽  
Morgan N Cash ◽  
James M Luther ◽  
...  
Keyword(s):  
Somatic Mutations ◽  
Mutational Analysis ◽  
Cushing Syndrome ◽  
Gene Mutations ◽  
Amplicon Sequencing ◽  
Formalin Fixed Paraffin ◽  
Mutation Profile ◽  
Somatic Gene ◽  
Formalin Fixed Paraffin Embedded ◽  
Aldosterone Producing Adenoma

Abstract Background Somatic gene mutations have been identified in only about half of cortisol-producing adenomas (CPA). Affected genes include PRKACA, GNAS, PRKAR1A, and CTNNB1. Objective To expand our understanding of the prevalence of somatic mutations in CPA from patients with overt Cushing syndrome (OCS) and “subclinical” mild autonomous cortisol excess (MACE), with an immunohistochemistry (IHC)‒guided targeted amplicon sequencing approach using formalin-fixed paraffin-embedded (FFPE) tissue. Method We analyzed FFPE adrenal tissue from 77 patients (n=12 men, 65 women) with either OCS (n=32) or MACE (n=45). Using IHC for 17α-hydroxylase/17,20-lyase (CYP17A1) and 3β-hydroxysteroid dehydrogenase (HSD3B2), we identified 78 CPA (32 OCS-CPA and 46 MACE-CPA). Genomic DNA was isolated from the FFPE CPA and subjected to targeted amplicon sequencing for identification of somatic mutations. Results Somatic mutations were identified in 71.8% (56/78) of the CPA. While PRKACA was the most frequently mutated gene in OCS-CPA (14/32, 43.8%), somatic genetic aberrations in CTNNB1 occurred in 56.5% (26/46) of the MACE-CPA. Most GNAS mutations were observed in MACE-CPA (5/7,71.4%). No mutations were observed in PRKAR1A. In addition to the known mutations, we identified one previously unreported mutation in PRKACA. Two patients with MACE harbored two adjacent tumors within the same adrenal gland: one patient had two CPA, and the other patient had a CPA and an aldosterone-producing adenoma (identified by IHC for aldosterone synthase). Conclusion Comprehensive FFPE IHC-guided gene-targeted sequencing approach identified somatic mutations in 71.8% of the CPA. OCS-CPA demonstrated a distinct mutation profile compared to MACE-CPA.

scholarly journals p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry.

1992 ◽  
Vol 40 (7) ◽  
pp. 1047-1051 ◽  
Author(s):  
B J Kerns ◽  
P A Jordan ◽  
M B Moore ◽  
P A Humphrey ◽  
A Berchuck ◽  
...  
Keyword(s):  
Breast Cancers ◽  
Fresh Tissue ◽  
P53 Overexpression ◽  
P53 Expression ◽  
Ovarian Cancers ◽  
Formalin Fixed Paraffin ◽  
Wide Range ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Formalin Fixed

Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.

scholarly journals Clinicopathologic Significance of Histologic Grade, Pgp, and P53 Expression in Canine Lymphoma

2013 ◽  
Vol 49 (3) ◽  
pp. 175-184 ◽  
Author(s):  
Ravinder S. Dhaliwal ◽  
Barbara E. Kitchell ◽  
EJ Ehrhart ◽  
Victor E. Valli ◽  
Nikolaos G. Dervisis
Keyword(s):  
Histologic Grade ◽  
Canine Lymphoma ◽  
P53 Expression ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Histologic Evaluation ◽  
Chemotherapy Protocol ◽  
First Remission ◽  
Resistance Markers ◽  
Significant Expression

To characterize the expression of P-glycoprotein (Pgp) and p53 in different histologic grades of canine multicentric lymphosarcoma (LSA), 31 cases of LSA without prior treatment were studied. The expression levels of the Pgp and p53 proteins were evaluated for their clinicopathologic significance among standard histologic evaluation. Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded archival samples of 31 previously untreated LSA cases to detect the expression of Pgp and p53. All dogs were subsequently treated with a combination chemotherapy protocol. Remission and survival durations were evaluated for correlation with histologic grade and presence of drug resistance markers. Of the 31 cases, 24 (80%) and 7 (22%) were positive for Pgp and p53, respectively. Overall, the median survival and duration of remission in the study was 246 days and 137 days, respectively. The National Cancer Institute working formulation histologic grade was not associated with either survival or duration of first remission (DOR). The Pgp protein expression and DOR and survival was not statistically significant. Expression of p53 was statistically correlated with survival.

scholarly journals Co-stimulatory and co-inhibitory immune markers in solid tumors with MET alterations

2021 ◽  
Vol 7 (2) ◽  
pp. FSO662
Author(s):  
Karen L Reckamp ◽  
Jasmine A McQuerry ◽  
Isa Mambetsariev ◽  
Rebecca Pharaon ◽  
Susan E Yost ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Checkpoint Inhibitors ◽  
Splice Site Mutation ◽  
Immune Markers ◽  
Site Mutation ◽  
Met Amplification ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Immune Profiling ◽  
Formalin Fixed

The implication of MET alterations in solid tumors and the immune microenvironment remains elusive. Formalin-fixed, paraffin-embedded samples of 21 patients with solid tumors harboring MET alterations were used for immunohistochemical staining. Extracted RNA was analyzed with the NanoString nCounter human PanCancer immune profiling panel (NanoString Technologies, Inc., WA, USA). Patients were diagnosed with lung (n = 10), breast (n = 5), genitourinary (n = 3) or colorectal cancer (n = 3). Eleven had a MET missense mutation, four had an exon 14 splice site mutation and six had MET amplification. CD6, CCL19, CD40LG, XCR1, MAGEA1, ATM and CCL19 genes were significantly differentially expressed in MET-altered cancers. MET alterations may have a role in various solid tumors as potential therapeutic targets and combination therapy candidates with immune checkpoint inhibitors.

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