Rapid and direct DNA extraction from saliva for personalized medicine

2010 ◽  
Author(s):  
Hoi-chu Yung
Keyword(s):  
Personalized Medicine ◽  
Dna Extraction ◽  
Direct Dna Extraction

Direct DNA extraction for PCR-mediated assays of soil organisms.

1995 ◽  
Vol 61 (11) ◽  
pp. 3972-3976 ◽  
Author(s):  
T Volossiouk ◽  
E J Robb ◽  
R N Nazar
Keyword(s):  
Dna Extraction ◽  
Soil Organisms ◽  
Direct Dna Extraction

scholarly journals Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing

2015 ◽  
Vol 53 (8) ◽  
pp. 2716-2719 ◽  
Author(s):  
K. Bjorn-Mortensen ◽  
J. Zallet ◽  
T. Lillebaek ◽  
A. B. Andersen ◽  
S. Niemann ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Whole Genome Sequencing ◽  
Dna Extraction ◽  
Genome Sequencing ◽  
Sequence Data ◽  
Whole Genome ◽  
Content Type ◽  
Direct Dna Extraction ◽  
Slow Growing

Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such asMycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

scholarly journals Culturing and direct DNA extraction find different fungi from the same ericoid mycorrhizal roots

2003 ◽  
Vol 160 (1) ◽  
pp. 255-272 ◽  
Author(s):  
Tamara R. Allen ◽  
Tony Millar ◽  
Shannon M. Berch ◽  
Mary L. Berbee
Keyword(s):  
Dna Extraction ◽  
Mycorrhizal Roots ◽  
Direct Dna Extraction

scholarly journals Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction

2010 ◽  
Vol 79 (2) ◽  
pp. 277-280 ◽  
Author(s):  
Ayman Al-Mariri ◽  
Nermeen Haj-Mahmoud
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Brucella Abortus ◽  
Bovine Milk ◽  
Ring Test ◽  
Chain Reaction ◽  
Purification System ◽  
Polymerase Chain ◽  
Direct Dna Extraction ◽  
Milk Ring Test

This study compares milk ring test and three different polymerase chain reaction techniques (direct DNA extraction by column purification system, alkaline DNA extraction, and filtrated milk), in order to identifyBrucella abortusinfection in bovine milk. Milk ring test sensitivity and specificity were 72% and 80%, respectively. While specificity of the three polymerase chain reaction techniques was 100%; sensitivity was 92%, 88% and 100%, respectively, for the three polymerase chain reaction procedures. We conclude that the filtered animal’s milk polymerase chain reaction is the best procedure to make the diagnosis ofB. abortusinfections.

Sign in / Sign up

Export Citation Format

Share Document