scholarly journals EVALUATION OF ALKALINE PRETREATMENT ON THE ENZYMATIC HYDROLYSIS OF CARNAUBA STRAW RESIDUE

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Alan De Oliveira Campos ◽  
Francinaldo Leite Da Silva ◽  
Emilianny Rafaely Batista Magalhães ◽  
Gorete Ribeiro De Macedo ◽  
Everaldo Silvino Dos Santos
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cellulosic Ethanol ◽  
Chemical Characterization ◽  
Crystallinity Index ◽  
Biomass Pretreatment ◽  
Cellulose Conversion ◽  
Hydrolysis Of ◽  
Potential Use ◽  
Second Generation Ethanol

Carnauba (Copernicia prunifera) straw residue generated from production of its wax is rich in cellulose, thus showing a potential use in the production of second generation ethanol. However, the high lignin and hemicellulose load associated with cellulose makes it difficult the enzymatic attack, thus having the need of an adequate pretreatment of this material. The objective of this study was to optimize the enzymatic hydrolysis of carnauba straw residue, focusing on the alkaline biomass pretreatment. Therefore, NaOH solutions at concentrations of 1.0% (w/v) (PA1), 2.0% (w/v) (PA2), 3.0% (w/v) (PA3) and 4.0% (w/v) (PA4) were used. The chemical and physical characterization of natural and pre-treated carnauba straw were according to the NREL, and DRX and FTIR performed analyzes. The materials chemical characterization showed that all the used pretreatments were able to remove a significant amount of lignin and hemicellulose, which can improve the enzymes access, favoring the increase of cellulose conversion. In relation to DRX analysis an increase in crystallinity index happens reaching up to 55.15% after the pretreatment PA4, which may be associated to the removal of hemicellulose and amorphous lignin, related to cellulose. After a period of 96 hours of enzymatic hydrolysis, the PA4 pretreated residue showed the best performance with a cellulosic conversion of 78%. Spite of a slightly lower performance of the residue that presented higher cellulose conversion, the pretreated material PA2 is an alternative to reduce costs in the cellulosic ethanol production.

Physicochemical characterization of residue from the enzymatic hydrolysis of sugarcane bagasse in a cellulosic ethanol process at pilot scale

2016 ◽  
Vol 94 ◽  
pp. 463-470 ◽  
Author(s):  
Fabrícia Farias de Menezes ◽  
Renan Henrique da Silva Fernandes ◽  
George Jackson de Moraes Rocha ◽  
Rubens Maciel Filho
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Cellulosic Ethanol ◽  
Physicochemical Characterization ◽  
Pilot Scale ◽  
Hydrolysis Of

scholarly journals Synthesis and spectral characterization of 2,2-diphenylethyl glucosinolate and HPLC-based reaction progress curve data for the enzymatic hydrolysis of glucosinolates by Sinapis alba myrosinase

Data in Brief ◽  
2017 ◽  
Vol 10 ◽  
pp. 151-181 ◽  
Author(s):  
Chase A. Klingaman ◽  
Matthew J. Wagner ◽  
Justin R. Brown ◽  
John B. Klecker ◽  
Ethan H. Pauley ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sinapis Alba ◽  
Spectral Characterization ◽  
Reaction Progress ◽  
Progress Curve ◽  
Hydrolysis Of

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

scholarly journals Effects of Biosurfactants on Enzymatic Saccharification and Fermentation of Pretreated Softwood

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3559 ◽  
Author(s):  
Alfredo Oliva-Taravilla ◽  
Cristhian Carrasco ◽  
Leif J. Jönsson ◽  
Carlos Martín
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pseudomonas Aeruginosa ◽  
Enzymatic Hydrolysis ◽  
Enzymatic Saccharification ◽  
Horse Chestnut ◽  
Yeast Growth ◽  
Cellulose Conversion ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

The enzymatic hydrolysis of cellulose is inhibited by non-productive adsorption of cellulases to lignin, and that is particularly problematic with lignin-rich materials such as softwood. Although conventional surfactants alleviate non-productive adsorption, using biosurfactants in softwood hydrolysis has not been reported. In this study, the effects of four biosurfactants, namely horse-chestnut escin, Pseudomonas aeruginosa rhamnolipid, and saponins from red and white quinoa varieties, on the enzymatic saccharification of steam-pretreated spruce were investigated. The used biosurfactants improved hydrolysis, and the best-performing one was escin, which led to cellulose conversions above 90%, decreased by around two-thirds lignin inhibition of Avicel hydrolysis, and improved hydrolysis of pretreated spruce by 24%. Red quinoa saponins (RQS) addition resulted in cellulose conversions above 80%, which was around 16% higher than without biosurfactants, and it was more effective than adding rhamnolipid or white quinoa saponins. Cellulose conversion improved with the increase in RQS addition up to 6 g/100 g biomass, but no significant changes were observed above that dosage. Although saponins are known to inhibit yeast growth, no inhibition of Saccharomyces cerevisiae fermentation of hydrolysates produced with RQS addition was detected. This study shows the potential of biosurfactants for enhancing the enzymatic hydrolysis of steam-pretreated softwood.

Effects of Rhamnolipids on Enzymatic Hydrolysis of Bamboo Biomass and Mechanism

2020 ◽  
Vol 14 (4) ◽  
pp. 453-460
Author(s):  
Ruyi Sha ◽  
Zhan Yu ◽  
Zhenzhen Wang ◽  
Edwin Menledy Gbor ◽  
Ligang Jiang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fluorescence Probe ◽  
Economic Losses ◽  
Dilute Acid ◽  
Site Of Action ◽  
The Stability ◽  
Surface Modified ◽  
Hydrolysis Of ◽  
Positive Effect

The lignin present in lignocellulose seriously affects the efficiency of cellulose enzymatic hydrolysis. In addition, lignin adsorbs high-cost cellulase, causing greater economic losses. Lignin can also disturb the site of action of cellulase and reduce the efficiency of hydrolysis. Therefore, if lignin is removed or surface modified before cellulose enzymatic hydrolysis, the enzymatic hydrolysis efficiency of lignocellulosic biomass will be greatly improved. In this paper, the cellulose enzymatic properties of bamboo biomass being treated with dilute acid and alkaline under the intervention of biosurfactant rhamnolipid were evaluated. The effects of rhamnolipids on the adsorption characterization of cellulose on pretreated bamboo were studied. Besides, the inter-communication between rhamnolipids and cellulose was investigated by fluorescence probe. The results showed that rhamnolipids could have a positive effect on the enzymatic hydrolysis of bamboo biomass by reducing the non-productive adsorption of cellulase on the surface of lignocellulose. The outcome illustrated that cellulase could be combined with rhamnolipids micelles, participating in the formation of rhamnolipids micelles, thereby increasing the internal hydrophobicity of the micelles, but could not change the properties of rhamnolipids micelles higher than one CMC (Critical Micelle Concentration). It can be seen that the interaction between rhamnolipids and cellulase is beneficial to enhance the stability and enzymatic activity of cellulase, thereby improving the enzymatic hydrolysis efficiency of cellulose in biomass. Based on these results, a theoretical knowledge about the mechanism of enhancing the enzymatic hydrolysis efficiency of lignocellulose by biosurfactants rhamnolipids is provided.

scholarly journals Reduction of surface area of lignin improves enzymatic hydrolysis of cellulose from hydrothermally pretreated wheat straw

RSC Advances ◽  
2014 ◽  
Vol 4 (69) ◽  
pp. 36591-36596 ◽  
Author(s):  
M. H. Sipponen ◽  
V. Pihlajaniemi ◽  
O. Pastinen ◽  
S. Laakso
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Surface Area ◽  
Lignin Content ◽  
Cellulose Conversion ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Pretreated Wheat Straw ◽  
Hydrolysis Of

24 h enzymatic hydrolysis (15 FPU g−1) of solid residues from wheat straw autohydrolysis. Cellulose conversion as a function of lignin content (left) or lignin surface area (right) in solid residues.

scholarly journals Characterization of Residual Lignin Obtained by the Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch Pulps

BioResources ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Yin Ying H'ng ◽  
Akiko Nakagawa-Izumi ◽  
Cheu Peng Leh ◽  
Atanu Kumar Das ◽  
Hiroshi Ohi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Empty Fruit Bunch ◽  
Residual Lignin ◽  
Hydrolysis Of
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