scholarly journals THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF PLANT CELLS

2020 ◽  
Vol 5 ◽  
pp. 28
Author(s):  
Sharifa Zaman ◽  
B. Fatima
Keyword(s):  
Mechanical Properties ◽  
Image Processing ◽  
Mechanical Response ◽  
Three Dimensional ◽  
Plant Cells ◽  
Quantitative Characterization ◽  
And Topology ◽  
Short Period ◽  
Dimensional Reconstruction

The mechanical properties (like sensory texture etc.) of plants/fruits directly depend on their microstructures. Therefore, it is very important to well understand the geometry and topology of cells in order to control the microstructure for better mechanical response. In this research, techniques of digital image processing and segmentation in conjunction with mathematical morphology models are used to visualize and analyze the 3D cells of potato. ImageJ and MATLAB are used throughout in this study. The labeled image stacks are essential for studying quantitative characterization of 3D cells, MATLAB is used to label each image stacks. By using MATLAB 12420 cells were segmented within a short period of time and labeled each cell uniquely.

A Direct and Quantitative Three-Dimensional Reconstruction of the Internal Structure of Disordered Mesoporous Carbon with Tailored Pore Size

2013 ◽  
Vol 19 (3) ◽  
pp. 745-750 ◽  
Author(s):  
Juan Balach ◽  
Flavio Soldera ◽  
Diego F. Acevedo ◽  
Frank Mücklich ◽  
César A. Barbero
Keyword(s):  
Pore Size ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Quantitative Characterization ◽  
Resorcinol Formaldehyde ◽  
Dual Beam ◽  
Dimensional Reconstruction ◽  
A New Technique

AbstractA new technique that allows direct three-dimensional (3D) investigations of mesopores in carbon materials and quantitative characterization of their physical properties is reported. Focused ion beam nanotomography (FIB-nt) is performed by a serial sectioning procedure with a dual beam FIB-scanning electron microscopy instrument. Mesoporous carbons (MPCs) with tailored mesopore size are produced by carbonization of resorcinol-formaldehyde gels in the presence of a cationic surfactant as a pore stabilizer. A visual 3D morphology representation of disordered porous carbon is shown. Pore size distribution of MPCs is determined by the FIB-nt technique and nitrogen sorption isotherm methods to compare both results. The obtained MPCs exhibit pore sizes of 4.7, 7.2, and 18.3 nm, and a specific surface area of ca. 560 m2/g.

Construction of plastic contact deformation maps on ceramics: a case study on aluminum nitride

1996 ◽  
Vol 54 ◽  
pp. 636-637
Author(s):  
D. L. Callahan
Keyword(s):  
Plastic Deformation ◽  
Aluminum Nitride ◽  
Mechanical Response ◽  
Three Dimensional ◽  
Bright Field Image ◽  
Perfectly Plastic ◽  
Contrast Analysis ◽  
Deformation Patterns

Modern polishing, precision machining and microindentation techniques allow the processing and mechanical characterization of ceramics at nanometric scales and within entirely plastic deformation regimes. The mechanical response of most ceramics to such highly constrained contact is not predictable from macroscopic properties and the microstructural deformation patterns have proven difficult to characterize by the application of any individual technique. In this study, TEM techniques of contrast analysis and CBED are combined with stereographic analysis to construct a three-dimensional microstructure deformation map of the surface of a perfectly plastic microindentation on macroscopically brittle aluminum nitride.The bright field image in Figure 1 shows a lg Vickers microindentation contained within a single AlN grain far from any boundaries. High densities of dislocations are evident, particularly near facet edges but are not individually resolvable. The prominent bend contours also indicate the severity of plastic deformation. Figure 2 is a selected area diffraction pattern covering the entire indentation area.

Lengths and topology of alveolar septal borders

1989 ◽  
Vol 67 (5) ◽  
pp. 1930-1940 ◽  
Author(s):  
E. H. Oldmixon ◽  
J. P. Butler ◽  
F. G. Hoppin
Keyword(s):  
Connective Tissue ◽  
Three Dimensional ◽  
Free Standing ◽  
And Topology ◽  
Section Area ◽  
Dimensional Reconstruction ◽  
Alveolar Duct ◽  
Mechanical Linkage ◽  
Alveolar Septa ◽  
Alveolar Parenchyma

To clarify the mechanics of alveolar parenchyma, we undertook a stereological and topological study in perfusion-fixed canine lungs of the borders of alveolar septa. We defined the principal borders as those along which one septum 1) joins two others (J), 2) joins one other at a distinct angle (B), or 3) joins no other structure (E). E and B borders are invariably reinforced with heavy connective tissue cables; J borders are not. Relative net lengths, determined from the number of traces per section area, were J, 45%; E, 19%; and B, 25%. These were remarkably constant over 10 canine lobes (5 animals, 4 volumes). Parenchyma, then, departs from the simple models that comprise only Js and Es. Bs are important; their net length exceeds that of Es. With lobe deflation, E shortened somewhat more than required to maintain geometric similarity, suggesting that the alveolar duct contracted disproportionately. A three-dimensional reconstruction was made from serial sections, and individual border segments were followed through the reconstruction. Typical lengths of individual J, B, and E borders were nearly equal. To characterize how the network of borders were interconnected, we counted the nodes at which they meet by class, e.g., EBE for the meeting of one B, two Es. The most common are JJJJ, 26%; EEEJ, 10%; EBJ, 24%; EBE, 8%; BBJJ, 12%. If parenchyma were constructed only from free-standing entrance rings and septal junctions, only JJJJ and EEEJ would be anticipated. The presence of EBJ, EBE, and BBJJ underscores parenchymal complexity. Only 7% of septa examined were bordered entirely by Js. Connective tissue cables were not confined to the alveolar duct's lumen but often extended to the primary septa at the periphery of the ductal unit. They rarely linked adjacent alveolar ducts; only 1 in 200 cable segments crossed from one duct to another. These observations support the concept that the parenchyma is an elastic network, characterized in part by a serial mechanical linkage from connective tissue cable to septal membrane to cable again.

Quantitative Characterization of Pore Arrangement in Pore Bands in Pressure Die Cast AZ91 Magnesium Alloy by Image Processing

2006 ◽  
Vol 514-516 ◽  
pp. 1477-1482 ◽  
Author(s):  
D. G. Leo Prakash ◽  
Doris Regener
Keyword(s):  
Image Processing ◽  
Magnesium Alloy ◽  
Programming Language ◽  
Az91 Magnesium Alloy ◽  
Quantitative Characterization ◽  
Processing Defect ◽  
Die Cast ◽  
Az91 Magnesium ◽  
Pore Arrangement

Microporosity is the major processing defect in pressure die cast AZ91 magnesium alloy. There is a big difference in the arrangement of pores in different regions of the castings. The present work explains the pore arrangement in pore bands and other regions. Quantification and characterization of pores in pore bands is expected to be useful to understand the process-propertymicrostructure correlation. A computational microstructural (image) analyzing technique has been developed by a programming language to quantify and analyze the micropores in pore bands. The pore band regions and the rest were separated and quantified. In addition, image analyzing technique was used to measure the clustering tendency of porosity in pore bands and it was compared with other regions.

X-ray topographic determination of the granular structure in a graphite mosaic crystal: a three-dimensional reconstruction

2000 ◽  
Vol 33 (4) ◽  
pp. 1023-1030 ◽  
Author(s):  
M. Ohler ◽  
M. Sanchez del Rio ◽  
A. Tuffanelli ◽  
M. Gambaccini ◽  
A. Taibi ◽  
...  
Keyword(s):  
Structural Parameters ◽  
Pyrolytic Graphite ◽  
Three Dimensional ◽  
X Ray ◽  
Dimensional Reconstruction ◽  
Mosaic Crystals ◽  
Spatial Locations ◽  
Crystalline Domains

Section topographs recorded at different spatial locations and at different rocking angles of a highly oriented pyrolytic graphite (HOPG) crystal allow three-dimensional maps of the local angular-dependent scattering power to be obtained. This is performed with a direct reconstruction from the intensity distribution on such topographs. The maps allow the extraction of information on local structural parameters such as size, form and internal mosaic spread of crystalline domains. This data analysis leads to a new method for the characterization of mosaic crystals. Perspectives and limits of applicability of this method are discussed.

Structural Characterization of the Cell Division Cycle in Strigomonas culicis, an Endosymbiont-Bearing Trypanosomatid

2014 ◽  
Vol 20 (1) ◽  
pp. 228-237 ◽  
Author(s):  
Felipe Lopes Brum ◽  
Carolina Moura Costa Catta-Preta ◽  
Wanderley de Souza ◽  
Sergio Schenkman ◽  
Maria Carolina Elias ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Host Cell ◽  
Light And Electron Microscopy ◽  
Three Dimensional ◽  
Symbiotic Bacterium ◽  
Single Copy ◽  
Cell Structures ◽  
Dimensional Reconstruction ◽  
Protozoan Cell

AbstractStrigomonas culicis (previously referred to as Blastocrithidia culicis) is a monoxenic trypanosomatid harboring a symbiotic bacterium, which maintains an obligatory relationship with the host protozoan. Investigations of the cell cycle in symbiont harboring trypanosomatids suggest that the bacterium divides in coordination with other host cell structures, particularly the nucleus. In this study we used light and electron microscopy followed by three-dimensional reconstruction to characterize the symbiont division during the cell cycle of S. culicis. We observed that during this process, the symbiotic bacterium presents different forms and is found at different positions in relationship to the host cell structures. At the G1/S phase of the protozoan cell cycle, the endosymbiont exhibits a constricted form that appears to elongate, resulting in the bacterium division, which occurs before kinetoplast and nucleus segregation. During cytokinesis, the symbionts are positioned close to each nucleus to ensure that each daughter cell will inherit a single copy of the bacterium. These observations indicated that the association of the bacterium with the protozoan nucleus coordinates the cell cycle in both organisms.

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