ANTI-INFLAMMATORY EFFECT OF SEEDS OF TAMARINDUS INDICA IN WISTAR RATS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (02) ◽  
pp. 49-53
Author(s):  
M. G Hivrale ◽  
◽  
A. A. Mali ◽  
D. D. Bandawane
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Second Phase ◽  
Tamarindus Indica ◽  
Reference Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Indian Medicine

Tamarindus indica L. is highly valued in Indian medicine for management of painful inflammation, diabetes and constipation. In the present study, the anti-inflammatory potential was evaluated using carrageenan induced rat paw edema model. The coarse seed powder was extracted with distilled water, ethyl acetate and methanol and submitted to chemical tests which revealed the presence of alkaloids, tannins, flavonoids, glycosides, phenolic compounds and steroids. All the three extracts i.e. aqueous, ethyl acetate and methanolic extract of Tamarindus indica seeds at 500 mg/kg showed significant anti-inflammatory activity in the second phase of inflammation. For comparison purpose, we used indomethacin (10 mg/kg) as a reference drug. The results showed that methanolic extract exhibited more promising anti-inflammatory activity compared to aqueous and ethyl acetate extracts.

STUDY OF ANTI-INFLAMMATORY ACTIVITY OF CASSIA AURICULATA LINN. LEAVES IN WISTAR RATS

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (11) ◽  
pp. 44-47
Author(s):  
A. A. Mali ◽  
◽  
M. G Hivrale ◽  
D. D. Bandawane ◽  
P. D Chaudhari
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Cassia Auriculata ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Pharmacological Model

The present study was undertaken to evaluate the anti-inflammatory activity of aqueous, methanolic, ethyl acetate and hydroalcoholic extracts of Cassia auriculata leaves. The study was carried out using the pharmacological model of carrageenan induced rat paw edema. Among all extracts methanolic extract showed maximum anti-inflammatory potential. Indomethacin (10mg/kg) was used as referencec ompound in the present study. The anti-inflammatory activity of methanolic extract may be due to presence alkaloids, flavonoids, tannins and steroids.

scholarly journals Anti-Inflammatory Activity of Chrozophora rottleri Extracts on Carrageenan-Induced Rat Paw Edema

2016 ◽  
Vol 3 ◽  
pp. 20-26
Author(s):  
Mallikarjuna Rao Talluri ◽  
Battu Ganga Rao ◽  
Y. Venkateswaea Rao
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Bioactive Molecules ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Isolation And Characterization ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

The present study was intended to evaluate Anti-inflammatory activity ofC. rottleriextracts (Hydroalcoholic, Methanol, Ethyl acetate and Hexane). The Anti-inflammatory activity ofC.rottleriextracts at doses of 125mg/kg, 250mg/kg and 500mg/kg using carrageenan induced rat paw edema model compared with standard drug (Indomethacin). The selected plant extracts significantly inhibited paw edema along with the standard drug Indomethacin. Of all extracts, methanol extract produced significant effect on reduction of increased paw thickness, hydro alcoholic and ethyl acetate extracts produced moderate percentage inhibition and hexane extract produced low level of percentage inhibition in reducing paw edema on carrageenan induced rats. In all extracts, methanol extract at a dose of 500mg/kg showed more percentage inhibition i.e . 53.47±2.19. From the results obtained during the study it is concluded thatC. rottlerihaving the bioactive molecule responsible for Anti-inflammatory activity by individually or by combination of different bio-active compounds present in it. Further is necessary for isolation and characterization of bioactive molecules which are responsible for the selected plant biological activities.

The Anti-inflammatory Activity of Scutellaria rivularis Extracts and Its Active Components, Baicalin, Baicalein and Wogonin

1996 ◽  
Vol 24 (01) ◽  
pp. 31-36 ◽  
Author(s):  
Chun-Ching Lin ◽  
Den-En Shieh
Keyword(s):  
Ethyl Acetate ◽  
Chloroform Extract ◽  
Inflammatory Activity ◽  
Inhibition Activity ◽  
Paw Edema ◽  
Active Components ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Five extracts (n-hexane, chloroform, ethyl acetate, n-butanol and water) of Scutellaria rivularis Benth. were evaluated for their anti-inflammatory activity against carrageenan-induced paw edema in rats and compared with indomethacin. The result indicated that chloroform extract proved to be the most effective in all of the extracts. Consequently, three major components (baicalin, baicalein and wogonin) of the chloroform extract were further tested for their anti-inflammatory activity using the same model. It was found that baicalin exhibits the greatest inhibition activity against carrageenan-induced rat paw edema.

scholarly journals FORMULATION AND EVALUATION OF TOPICAL ANTI-INFLAMMATORY HERBAL GEL

2019 ◽  
pp. 252-255
Author(s):  
MAHENDRA A GIRI ◽  
RASIKA D BHALKE
Keyword(s):  
Methanolic Extract ◽  
Skin Irritation ◽  
Storage Conditions ◽  
Reference Drug ◽  
Edema Volume ◽  
Rat Paw Edema ◽  
Ich Guidelines ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

Objective: The present investigation aims at development and evaluation of herbal anti-inflammatory gel using of methanolic extract from Emblica officinalis fruits (MEEOF) and Aegle marmelos fruits (MEAMF). Methods: The gels were prepared using Carbopol 934, various concentrations of MEEOF, MEAMF, propylene glycol 400, methylparaben, propylparaben, and required amount of distilled water. Then, skin pH (6.8-7) was maintained by dropwise addition of triethanolamine. Prepared formulations were evaluated for physical appearance, pH, spreadability, viscosity and homogeneity, skin irritation on animal model (rabbit), and anti-inflammatory activity using carrageenan-induced rat paw edema model on albino Wistar rats of either sex (150–200 g). Change in edema volume of the rat hind paw was measured, and percent inhibition was calculated. ICH guidelines have followed for stability studies. Results: Results reveal that gel showed good appearance, homogeneity, and spreadability. Viscosity is ranging between 4200 and 4500 centipoises. All formulations have shown no skin irritation to animals. Formulations F4 and F5 significantly inhibited the inflammation to the extent of 56.66%, 61.66% at 3 h and 59.21%, 63.15% at 4 h, respectively, while the reference drug reduced the inflammation by 66.66% at 3 h and 76.31% at 4 h. The preparation was stable under normal storage conditions and did not produce any skin irritation, i.e., erythema and edema when applied over the skin on storage. Conclusion: It was concluded that anti-inflammatory effect of F4 and F5 was comparable to standard.

scholarly journals Leaves of Stereospermum suaveolens DC Exhibit Anti-inflammatory and Anti-arthritic Potential Action in Experimental Animals

2021 ◽  
pp. 164-175
Author(s):  
R. R. Chanshetti ◽  
D. D. Bandawane
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Research Work ◽  
Ethyl Acetate Fraction ◽  
Significant Inhibition ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Aim: The experimental investigation of current research work was to identify traditional rich claim of Stereospermum suaveolens DC leaves for anti-inflammatory and anti-arthritic potential action in animals. Study design: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves evaluated at 125mg/kg, 250mg/kg and 500mg/kg (p.o.) doses for anti-inflammatory and anti-arthritic activity. Methodology: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves was evaluated for phytochemical investigation for total flavonoid content using UV spectroscopy and TLC study. Carrageenan induced rat paw edema (Acute method) and Freund’s complete adjuvant (FCA) induced chronic arthritis in wistar rats were used as an animal models to claim Stereospermum suaveolens DC leaves for anti-inflammatory and anti-arthritic potential.  The rat paw volume and percentage inhibition of the paw edema were evaluated for anti-inflammatory activity. The assessments of arthritis in rats were measured by haematological values and radiological examinations. Result: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves showed presence of total flavonoids and saponins. The significant inhibition in paw volume and edema (p < .01) obtained at 250mg/kg and 500mg/kg oral dose. These obtained results were established confirmation outcome for presence of rich flavonoid contents in Stereospermum suaveolens DC leaves and provides valuable source of bioactive phytocomponents.  Conclusion: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves  showed significant inhibition of inflammatory reaction as compared to standard drug indomethacin Sterospermum Suaveolens DC leaves were showed potential therapeutic role in treatment of inflammation and arthritis cases.

scholarly journals Bioactivity-Guided Investigation of the Anti-Inflammatory Activity of Hippophae rhamnoides Fruits

Planta Medica ◽  
2017 ◽  
Vol 84 (01) ◽  
pp. 26-33 ◽  
Author(s):  
Dóra Rédei ◽  
Norbert Kúsz ◽  
Nikoletta Jedlinszki ◽  
Gábor Blazsó ◽  
István Zupkó ◽  
...  
Keyword(s):  
Hippophae Rhamnoides ◽  
Inflammatory Activity ◽  
Hippophaë Rhamnoides ◽  
Rat Paw Edema ◽  
In Vivo Animal Models ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
First Time ◽  
Peel Extract

AbstractAccording to modern ethnobotanical records, the fruit of Hippophae rhamnoides is effective in the treatment of different allergic symptoms. In order to obtain pharmacological evidence for this observation, the fruit was investigated for anti-inflammatory activity using in vivo animal models. Aqueous and 70% MeOH extracts were tested in 48/80-induced rat paw edema assay after oral administration, and it was found that the 70% MeOH extract (500 mg/kg) reduced significantly edema volume (0.660 ± 0.082 mL vs. control 0.935 ± 0.041 mL). Extracts of different parts of the fruit (pulp, peel, seed) were investigated in the same assay, and the peel extract was shown to exhibit maximum edema-reducing effect (0.470 ± 0.124 mL vs. control 0.920 ± 0.111 mL). This extract was used to elucidate the mode of action. Different inflammation inducers (serotonin, histamine, dextran, bradykinin, and carrageenan) were applied in the rat paw model, but the extract inhibited only the compound 48/80 elicited inflammation. The active extract was then fractionated by solvent-solvent partitioning and chromatographic methods with the guidance of the 48/80-induced anti-inflammatory assay, and the main compounds responsible for the activity were identified as ursolic acid and oleanolic acid. Our data suggest that the activity is most probably based on a membrane stabilizing effect caused by the inhibition of degranulation of mast cells. Moreover, previously unknown 2,5-bis-aryl-3,4-dimethyltetrahydrofuran lignans, nectandrin B, fragransin A2, and saucernetindiol were isolated and identified from H. rhamnoides for the first time.

scholarly journals SYNTHESIS, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ETHYL 2-(2-CYANO-3- (SUBSTITUTED PHENYL)ACRYLAMIDO)-4,5-DIMETHYLTHIOPHENE-3-CARBOXYLATES

2017 ◽  
Vol 10 (7) ◽  
pp. 95 ◽  
Author(s):  
Madhavi K ◽  
Sree Ramya G
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Properties ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Objective: Objective of the study was to synthesize and evaluate a series of novel compounds, ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)- 4,5-dimethylthiophene-3-carboxylates, for in vitro antioxidant and in vivo anti-inflammatory activities.Methods: Ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5-dimethylthiophene-3-carboxylates were synthesized by knoevenagel condensation of active methylene group of ethyl 2-(2-cyanoacetamido)-4,5-dimethylthiophene-3-carboxylate with substituted benzaldehydes. The synthesized compounds were evaluated for their in vitro antioxidant properties in three different models, viz., reduction of 1,1-diphenyl-2-pycrylhydrazyl free radical, scavenging of nitric oxide free radical, and ferric ion-induced lipid peroxidation using rat brain homogenate. Few selected compounds with good antioxidant properties were pharmacologically evaluated for anti-inflammatory activity by carrageenan-induced rat paw edema model.Results: Clean and efficient synthetic procedure was used for the preparation of series of compounds. The structures of synthesized compounds were confirmed by infrared, 1H nuclear magnetic resonance and mass spectra. The antioxidant activity data revealed that the compounds of ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5-dimethylthiophene-3-carboxylate containing phenolic substitution showed greater antioxidant activity. Hence, the active compounds were evaluated for anti-inflammatory activity and found to possess good activity. The percentage inhibition of rat paw edema obtained for the evaluated compounds was in the range of 70.2-83.1, comparable to the standard drug diclofenac (85.0%).Conclusion: The use of inexpensive, eco-friendly and readily available reagents, easy work-up and high purity of products makes the procedure a convenient and robust method for the synthesis of title compounds. The compounds of ethyl 2-(2-cyano-3-(substituted phenyl)acrylamido)-4,5- dimethylthiophene-3-carboxylate containing phenolic substitution showed greater antioxidant and anti-inflammatory activities.

scholarly journals Ufasomes Mediated Cutaneous Delivery of Dexamethasone: Formulation and Evaluation of Anti-Inflammatory Activity by Carrageenin-Induced Rat Paw Edema Model

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Rajkamal Mittal ◽  
Arvind Sharma ◽  
Sandeep Arora
Keyword(s):  
Oleic Acid ◽  
Entrapment Efficiency ◽  
Inflammatory Activity ◽  
Molar Ratio ◽  
Paw Edema ◽  
Rat Paw Edema ◽  
Transmission Electron ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Rat Paw

The purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher () as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema () was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

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