scholarly journals Isolation and Identification of Candida from the Oral Cavity

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Smitha Byadarahally Raju ◽  
Shashanka Rajappa
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Hiv Infection ◽  
Antifungal Agents ◽  
Isolation And Identification ◽  
Diagnosis And Management ◽  
Oral Candidosis ◽  
Reliable Methods

Various techniques are available for the isolation of Candida within the oral cavity. Such methods play an important role in the diagnosis and management of oral candidosis. The growing importance of Candida is in part related to the emergence of HIV infection and the more widespread use of immunosuppressive chemotherapy. Along with the Candida albicans there has been a greater recognition of the importance of the nonalbicans Candida species in oral candidosis. Identification of infecting strains of Candida is important because isolates of Candida species differ widely, both in their ability to cause infection and also in their susceptibility to antifungal agents. Thus this review provides an overview of the reliable methods of candidal isolation and identification of isolates from the oral cavity.

scholarly journals Study the Inhibitory effect of Rose and blossom bitter orange flowers extracts against different types of bacteria isolated from the oral cavity

2014 ◽  
Vol 11 (2) ◽  
pp. 795-802
Author(s):  
Baghdad Science Journal
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Cold Water ◽  
Inhibitory Effect ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Bitter Orange ◽  
Wide Range ◽  
Different Types ◽  
Amoxicillin Clavulanic Acid

The study in duded isolation and identification of microbial isolates from oral cavity to 10 volunteers, diagnosed within the three groups: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp. and Candida albicans . The sensitivity test of all isolates bacteria Streptococcus spp. , S. aureus and S. epidermidis showed high resistance to Ampicillin(100)%,followed Methicillin (88.88)% and Amoxicillin / clavulanic acid(77.77)%, while the resistance for each of Vancomycin and Amoxicillin were (66.66)%, and the resistance to Erythromycin and Pencillin (55.55)% to each of them. The results showed less resistance to Trimethoprim (22.22)% and Cefalotine (11.11)% of all bacteria isolate. Investigation of the presence of active compounds in each of the hot and cold (water and alcoholic) extracts flowers Rose and flowers blossom bitter orange ( new preparatory 2012) (Alkaloids, Quartet alkaloids, sugars, Saponine, Flavones and comarins ) was carried out includes. While it was all kinds of extracts does not contain resins. The results showed the presence of active compounds (Sugars, Flavones and comarins) in old extracts that preparation after a year (2011). PH values of the plant extracts hot and cold (water and alcoholic) for each of orange flowers blossom and Rose flowers (Old and new preparatory) with a wide range ranging between (3.6 -6.4). All extracts hot and cold (alcoholic) (new preparatory 2012) showed most effective towards Streptococcus spp., S. epidermidis and Candida albicans, while the extracts show weak effective against S. aureus. Hot alcohol extracts of Rose (old preparatory 2011) showed most effective towards S. aureus and S. epidermidis only, while the other extracts of old perpetration show weak effective against Streptococcus spp. and C. albicans.

Surface microhardness of different restorative materials exposed to candida albicans biofilm isolated from the oral cavity of hiv-infected children

2019 ◽  
Vol 4 (1) ◽  
pp. 87-91
Author(s):  
Leandro COSTA ◽  
Larissa SOARES-SILVA ◽  
Paulini Malfei De C. COSTA ◽  
Adrielle MANGABEIRA ◽  
Maristela PORTELA ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Surface Microhardness ◽  
Restorative Materials

Candida spp. In linear gingival erythema lesions in HIV- infeceted children: reports of six cases

2013 ◽  
Author(s):  
Maristela Barbosa Portela,
Keyword(s):  
Case Report ◽  
Hiv Infection ◽  
Pediatric Patients ◽  
Predictive Marker ◽  
Etiologic Agent ◽  
Candida Spp ◽  
Oral Manifestation ◽  
Oral Candidosis ◽  
Positive Growth ◽  
Microbiological Analyses

Linear gingival erythema (LGE), formally referred as HIV-gingivitis, is the most common form of HIV-associated periodontal disease in HIV-infection. These lesions were recently evaluated as a possible form of erythematous oral candidosis, mainly caused by Candida albicans. Other species are also being associated such as C. tropicalis, C. stellatoidea, C. krusei, C. parapsilosis, C. glabrata and C. dubliniensis, that was identified in some HIV-infected subjects. This case report demonstrates the presence of typical LGE lesions in six HIV-infected children, also investigates the etiologic agent by microbiological exams and correlates this oral manifestation with patients’ systemic conditions. Microbiological analyses showed positive growth for Candida spp in all patients, all of whom had severe imunessupression. After antifungal medication, the regression of lesions could be note. The presence of LGE in pediatric patients with AIDS may indicate its feature as a predictive marker in progression of HIV-infection in children.

scholarly journals Sublethal Injury and Resuscitation of Candida albicans after Amphotericin B Treatment

2003 ◽  
Vol 47 (4) ◽  
pp. 1200-1206 ◽  
Author(s):  
Robert S. Liao ◽  
Robert P. Rennie ◽  
James A. Talbot
Keyword(s):  
Cell Death ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Antifungal Agents ◽  
Sybr Green ◽  
Sequential Treatment ◽  
Tetrazolium Salt ◽  
Fungal Cell ◽  
Sublethal Injury

ABSTRACT Amphotericin B treatment was previously shown to inhibit Candida albicans reproduction and reduce the fluorescence of vitality-specific dyes without causing a corresponding increase in the fluorescence of the mortality-specific dyes bis-(1,3-dibutylbarbituric acid)trimethine oxonol and SYBR Green Ι. In the present study, we have confirmed these results and have shown that the numbers of CFU are reduced by 99.9% by treatment with 0.5 μg of amphotericin B per ml for 10 h at 35°C. This reduction was not due to fungal cell death. First, the level of reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide increased in the presence of concentrations of amphotericin B that caused greater than 90% reductions in the numbers of CFU. Second, fungal cells treated with amphotericin B at a concentration of 0.5 μg/ml were resuscitated by further incubation at 22°C for 15 h in the continued presence of amphotericin B. Third, recovery of the ability to replicate was prevented by sequential treatment with 20 μg of miconazole per ml, which also increased the fluorescence of mortality-specific dyes to near the maximal levels achieved with 0.9 μg of amphotericin B per ml. Sequential treatment with fluconazole and flucytosine did not increase the levels of staining with the mortality-specific dyes. Itraconazole was less effective than ketoconazole, which was less effective than miconazole. The practice of equating the loss of the capacity of C. albicans to form colonies with fungal cell death may give incorrect results in assays with amphotericin B, and the results of assays with caution with other antifungal agents that are lipophilic or that possess significant postantifungal effects may need to be interpreted.

scholarly journals Kinetic characterisation and inhibitor sensitivity of Candida albicans and Candida auris recombinant AOX expressed in a self-assembled proteoliposome system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alice C. Copsey ◽  
Mario R. O. Barsottini ◽  
Benjamin May ◽  
Fei Xu ◽  
Mary S. Albury ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Future Development ◽  
Therapeutic Target ◽  
Fungicide Resistance ◽  
Alternative Oxidase ◽  
Antifungal Agents ◽  
Candida Auris ◽  
Novel Treatments ◽  
Membrane Bound ◽  
Self Assembled

AbstractCandidemia caused by Candida spp. is a serious threat in hospital settings being a major cause of acquired infection and death and a possible contributor to Covid-19 mortality. Candidemia incidence has been rising worldwide following increases in fungicide-resistant pathogens highlighting the need for more effective antifungal agents with novel modes of action. The membrane-bound enzyme alternative oxidase (AOX) promotes fungicide resistance and is absent in humans making it a desirable therapeutic target. However, the lipophilic nature of the AOX substrate (ubiquinol-10) has hindered its kinetic characterisation in physiologically-relevant conditions. Here, we present the purification and expression of recombinant AOXs from C. albicans and C. auris in a self-assembled proteoliposome (PL) system. Kinetic parameters (Km and Vmax) with respect to ubiquinol-10 have been determined. The PL system has also been employed in dose–response assays with novel AOX inhibitors. Such information is critical for the future development of novel treatments for Candidemia.

Stridor in patients with HIV infection

1995 ◽  
Vol 109 (12) ◽  
pp. 1197-1199 ◽  
Author(s):  
R. B. S. Laing ◽  
P. J. C. Wardrop ◽  
P. D. Welsby ◽  
R. P. Brettle
Keyword(s):  
Hiv Infection ◽  
Broad Spectrum ◽  
Antifungal Agents ◽  
Opportunistic Infections ◽  
Upper Airways ◽  
Airway Intervention ◽  
Advanced Immunodeficiency ◽  
Laryngeal Tumours ◽  
Antibacterial And Antifungal

AbstractThe immunodeficiency which results from HIV infection is associated with a range of opportunistic infections and tumours which may present with the symptoms of upper airways disease. This paper presents three cases of stridor from different causes in patients with HIV infection, all of whom recovered following treatment. The management of this problem requires consideration of the likely aetiology which, in those with advanced immunodeficiency, includes bacterial and fungal laryngitis and epiglottitis as well as rapidly growing laryngeal tumours. Recommendations for the treatment of those with HIV infection who present with severe or rapidonset stridor should include a combination of aggressive airway intervention and broad-spectrum antibacterial and antifungal agents. Laryngeal biopsy for histology and culture is particularly important for those patients who fail to respond to the aforementioned treatment.

scholarly journals CaNdt80 Is Involved in Drug Resistance in Candida albicans by Regulating CDR1

2004 ◽  
Vol 48 (12) ◽  
pp. 4505-4512 ◽  
Author(s):  
Chia-Geun Chen ◽  
Yun-Liang Yang ◽  
Hsin-I Shih ◽  
Chia-Li Su ◽  
Hsiu-Jung Lo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Candida Albicans ◽  
Type Strain ◽  
Antifungal Agents ◽  
Wild Type Strain ◽  
Efflux Pump ◽  
Antifungal Drugs ◽  
Galactosidase Activity ◽  
Wild Type

ABSTRACT Overexpression of CDR1, an efflux pump, is one of the major mechanisms contributing to drug resistance in Candida albicans. CDR1 p-lacZ was constructed and transformed into a Saccharomyces cerevisiae strain so that the lacZ gene could be used as the reporter to monitor the activity of the CDR1 promoter. Overexpression of CaNDT80, the C. albicans homolog of S. cerevisiae NDT80, increases the β-galactosidase activity of the CDR1 p-lacZ construct in S. cerevisiae. Furthermore, mutations in CaNDT80 abolish the induction of CDR1 expression by antifungal agents in C. albicans. Consistently, the Candt80/Candt80 mutant is also more susceptible to antifungal drugs than the wild-type strain. Thus, the gene for CaNdt80 may be the first gene among the regulatory factors involved in drug resistance in C. albicans whose function has been identified.

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